SSGCID: Target Page

CrneC.19338.a

Uncharacterized protein

CENTER ID:	CrneC.19338.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19338.a.B1.GE40929	full length		1	345

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_01162
UniProt:	J9VL68

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAMSKQRKVL TLEHFIIDTF TRLGNDGIER PVEQPDQIGG GGTYAIIGAR AFLPPTQLGM IVDYTPSTFP QALKDALESY GNEMWAFRER TDGLPTARAV NRYDGETRGF EYLYKPVLLS PNSLKGTHFE DIVPSVIHFI SYPAPRAGII LDEINDFRIS KGWSPLIVWE PEDESLEVVT SIASHVDILG PNQNEALRLY SLLTDPTFTD DDFKAVLTKI CRSLAYLRPR IGAVIRAGHL GCCYASADPR GYRPEVKWVP AYWNKERDGY EGKVVDPTGA GNAFMGGIAA ALSEGKSLDE AVRWGSVAAS FAIEQNGLPQ LTKTSDGPEL WNGQNPQERL TALSM

NT Sequence

atggctatgt ccaagcaacg gaaagtgttg accctggaac acttcatcat tgacactttc actcgccttg gtaatgatgg cattgaaaga cccgtagagc agcccgatca gataggggga ggaggcacct acgcgataat tggtgcgcgt gcattccttc cacccactca actgggcatg attgtagatt acacaccttc aacatttcct caagctctca aagatgcact ggagtcctac ggaaatgaga tgtgggcttt tagagagaga actgacgggc tcccaactgc aagagcagtc aaccggtatg acggcgagac aagagggttt gaatatctat acaagcctgt tcttctttca ccaaactctc tcaaagggac gcattttgaa gacattgtgc cttctgtaat tcatttcatc tcttaccctg ctccccgggc tgggataata ctcgatgaaa tcaacgattt cagaatttcc aaaggatggt ctcctctcat cgtttgggag ccagaggatg agtcattgga ggtagtcact tccatagcgt cccatgtcga tatcttggga ccaaaccaga acgaagcgct ccgcctgtat tctctcttaa cagatcccac cttcacggat gatgatttta aagctgttct caccaaaatt tgccgctccc tcgcatatct gcgacctcgc ataggtgctg tgatccgcgc aggtcatttg ggatgctgtt atgcttccgc cgacccaaga ggatatcgac cagaagtaaa gtgggtccca gcctattgga acaaagagag ggatggctat gagggaaagg tagtagaccc caccggagca ggcaatgcat tcatgggagg aatagctgcc gcactgtcgg aaggaaagag tttagacgag gctgtgaggt ggggtagtgt tgcggcgtct ttcgccattg aacagaatgg tttgcctcag cttaccaaga cttccgatgg cccggaactc tggaatgggc aaaatccaca ggagaggctg acagccctgt ccatgtga

Details for CrneC.19338.a.B1.GE40929

HARVESTED ON:	12/20/2016
SEQUENCED ON:	12/27/2016
EXPECTED MW:	39kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA MSKQRKVLTL EHFIIDTFTR LGNDGIERPV EQPDQIGGGG TYAIIGARAF LPPTQLGMIV DYTPSTFPQA LKDALESYGN EMWAFRERTD GLPTARAVNR YDGETRGFEY LYKPVLLSPN SLKGTHFEDI VPSVIHFISY PAPRAGIILD EINDFRISKG WSPLIVWEPE DESLEVVTSI ASHVDILGPN QNEALRLYSL LTDPTFTDDD FKAVLTKICR SLAYLRPRIG AVIRAGHLGC CYASADPRGY RPEVKWVPAY WNKERDGYEG KVVDPTGAGN AFMGGIAAAL SEGKSLDEAV RWGSVAASFA IEQNGLPQLT KTSDGPELWN GQNPQERLTA LSM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta tgtccaagca acggaaagtg ttgaccctgg aacacttcat cattgacact ttcactcgcc ttggtaatga tggcattgaa agacccgtag agcagcccga tcagataggg ggaggaggca cctacgcgat aattggtgcg cgtgcattcc ttccacccac tcaactgggc atgattgtag attacacacc ttcaacattt cctcaagctc tcaaagatgc actggagtcc tacggaaatg agatgtgggc ttttagagag agaactgacg ggctcccaac tgcaagagca gtcaaccggt atgacggcga gacaagaggg tttgaatatc tatacaagcc tgttcttctt tcaccaaact ctctcaaagg gacgcatttt gaagacattg tgccttctgt aattcatttc atctcttacc ctgctccccg ggctgggata atactcgatg aaatcaacga tttcagaatt tccaaaggat ggtctcctct catcgtttgg gagccagagg atgagtcatt ggaggtagtc acttccatag cgtcccatgt cgatatcttg ggaccaaacc agaacgaagc gctccgcctg tattctctct taacagatcc caccttcacg gatgatgatt ttaaagctgt tctcaccaaa atttgccgct ccctcgcata tctgcgacct cgcataggtg ctgtgatccg cgcaggtcat ttgggatgct gttatgcttc cgccgaccca agaggatatc gaccagaagt aaagtgggtc ccagcctatt ggaacaaaga gagggatggc tatgagggaa aggtagtaga ccccaccgga gcaggcaatg cattcatggg aggaatagct gccgcactgt cggaaggaaa gagtttagac gaggctgtga ggtggggtag tgttgcggcg tctttcgcca ttgaacagaa tggtttgcct cagcttacca agacttccga tggcccggaa ctctggaatg ggcaaaatcc acaggagagg ctgacagccc tgtccatgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat