CrneC.19334.a

NAD kinase, variant

CENTER ID: CrneC.19334.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19334.a.B1.GE40926 full length 1 449
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19334.a.B1.PS38301 full length 1 449

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_04314
UniProt: T2BPF7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPGRRIVRSR IQHVLIVTKV NDNQLVSLTR ELALHLMQKS PSIPKGTNSR YGTASLGRGM VVYVDSEFET SKAFDVPGLQ RDHPELFEPM ISNRSSSSLS SMNTLNESST DEEPVTSGSS TPVGEGQLRY WTNKLCSNTP HLFDLVITLG GDGTVLYTSW LFQRIVPPVL PFALGSLGFL TKFDFKDYKE IIDKVIRDGI RVSLRMRFCC TVYRTSTPGN IDGPKAKKRR IIKDGSASAL KKRVHKSGWE SLEDEEADSH LSDAGSDEDV IMRHSTRPEE QFEVLNELVV DRGPNSAMSS LELFGDEYHL TTVQADGLTV STPTGSTAYS LSAGGSLTSP QTSNILITPI CPHTLSFRPV VLEDSIDIRV CVPFDSRTTA WTSFDGRSRL ELKQGDHIKV TASRYPFPII LYADKSFPDW ASSLSRKLRW NERERQKPYV LVEEARQQK
NT Sequence
atgccaggaa gaaggattgt gcgctctcga atacagcatg ttttgatcgt cactaaagtg aatgacaatc aacttgtctc tcttacaagg gagctggctc ttcatctcat gcaaaagagc ccttcgatac ccaaaggaac caacagtcgt tatggcacag catcacttgg gagaggcatg gtggtctatg tagactctga gtttgagact tcaaaggcgt tcgatgtccc gggcttgcag cgagatcatc cggagctttt tgagcccatg attagcaatc gctcttcgtc ctctctctcg tccatgaaca ctctaaatga gtcttctaca gacgaggagc cagtcacctc ggggtcatcg accccagtcg gtgagggaca gctgaggtac tggaccaaca agctttgttc aaacactcct cacttatttg acctcgtcat aacactcgga ggtgatggga cagtcctata tacttcttgg cttttccaac gaattgttcc tcctgtcctt ccctttgccc ttggctccct cggtttcttg accaaattcg atttcaagga ctacaaagag ataattgaca aggttattcg tgatgggata cgagtcagcc ttcgtatgcg tttttgctgt acagtatatc gtacgagcac tcctggcaat attgacggcc ccaaggccaa aaagcggcga atcatcaagg acggcagcgc cagtgccctt aagaaaaggg ttcataagag tggctgggag agcttggaag atgaagaagc agattcacat ttgtctgatg ctgggagtga cgaagatgta atcatgcgtc attcaacaag gcccgaagaa cagtttgaag tcctcaatga gctagtagtg gatagaggtc ctaattcagc catgagctcc ttggagctat ttggtgacga atatcatttg acaactgtac aagcagacgg gctcacggtt tcaacaccca ctggttctac ggcctactcc ttatcagccg gtggatcact cacctcccct caaacatcta acatcctcat aactccaatc tgtcctcaca cgctttcctt cagacctgtc gtcttggagg atagcatcga tattcgggtt tgtgtgccct tcgattcgag gacaacggct tggaccagct ttgatggaag gagtcgcctg gagttgaaac agggagatca tattaaagtt acagcctcga gatatccgtt cccaattata ttgtatgccg acaaatcctt cccagactgg gcatcttcgc tctcacgaaa gttgcgatgg aacgaaaggg agagacagaa gccatatgtg cttgttgaag aagcccgtca gcagaaataa
Details for CrneC.19334.a.B1.GE40926
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 51kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMP GRRIVRSRIQ HVLIVTKVND NQLVSLTREL ALHLMQKSPS IPKGTNSRYG TASLGRGMVV YVDSEFETSK AFDVPGLQRD HPELFEPMIS NRSSSSLSSM NTLNESSTDE EPVTSGSSTP VGEGQLRYWT NKLCSNTPHL FDLVITLGGD GTVLYTSWLF QRIVPPVLPF ALGSLGFLTK FDFKDYKEII DKVIRDGIRV SLRMRFCCTV YRTSTPGNID GPKAKKRRII KDGSASALKK RVHKSGWESL EDEEADSHLS DAGSDEDVIM RHSTRPEEQF EVLNELVVDR GPNSAMSSLE LFGDEYHLTT VQADGLTVST PTGSTAYSLS AGGSLTSPQT SNILITPICP HTLSFRPVVL EDSIDIRVCV PFDSRTTAWT SFDGRSRLEL KQGDHIKVTA SRYPFPIILY ADKSFPDWAS SLSRKLRWNE RERQKPYV
Validated NT Sequence
agcacatatg gcttctgtct ctccctttcg ttccatcgca actttcgtga gagcgaagat gcccagtctg ggaaggattt gtcggcatac aatataattg ggaacggata tctcgaggct gtaactttaa tatgatctcc ctgtttcaac tccaggcgac tccttccatc aaagctggtc caagccgttg tcctcgaatc gaagggcaca caaacccgaa tatcgatgct atcctccaag acgacaggtc tgaaggaaag cgtgtgagga cagattggag ttatgaggat gttagatgtt tgaggggagg tgagtgatcc accggctgat aaggagtagg ccgtagaacc agtgggtgtt gaaaccgtga gcccgtctgc ttgtacagtt gtcaaatgat attcgtcacc aaatagctcc aaggagctca tggctgaatt aggacctcta tccactacta gctcattgag gacttcaaac tgttcttcgg gccttgttga atgacgcatg attacatctt cgtcactccc agcatcagac aaatgtgaat ctgcttcttc atcttccaag ctctcccagc cactcttatg aacccttttc ttaagggcac tggcgctgcc gtccttgatg attcgccgct ttttggcctt ggggccgtca atattgccag gagtgctcgt acgatatact gtacagcaaa aacgcatacg aaggctgact cgtatcccat cacgaataac cttgtcaatt atctctttgt agtccttgaa atcgaatttg gtcaagaaac cgagggagcc aagggcaaag ggaaggacag gaggaacaat tcgttggaaa agccaagaag tatataggac tgtcccatca cctccgagtg ttatgacgag gtcaaataag tgaggagtgt ttgaacaaag cttgttggtc cagtacctca gctgtccctc accgactggg gtcgatgacc ccgaggtgac tggctcctcg tctgtagaag actcatttag agtgttcatg gacgagagag aggacgaaga gcgattgcta atcatgggct caaaaagctc cggatgatct cgctgcaagc ccgggacatc gaacgccttt gaagtctcaa actcagagtc tacatagacc accatgcctc tcccaagtga tgctgtgcca taacgactgt tggttccttt gggtatcgaa gggctctttt gcatgagatg aagagccagc tcccttgtaa gagagacaag ttgattgtca ttcactttag tgacgatcaa aacatgctgt attcgagagc gcacaatcct tcttcctggc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMP GRRIVRSRIQ HVLIVTKVND NQLVSLTREL ALHLMQKSPS IPKGTNSRYG TASLGRGMVV YVDSEFETSK AFDVPGLQRD HPELFEPMIS NRSSSSLSSM NTLNESSTDE EPVTSGSSTP VGEGQLRYWT NKLCSNTPHL FDLVITLGGD GTVLYTSWLF QRIVPPVLPF ALGSLGFLTK FDFKDYKEII DKVIRDGIRV SLRMRFCCTV YRTSTPGNID GPKAKKRRII KDGSASALKK RVHKSGWESL EDEEADSHLS DAGSDEDVIM RHSTRPEEQF EVLNELVVDR GPNSAMSSLE LFGDEYHLTT VQADGLTVST PTGSTAYSLS AGGSLTSPQT SNILITPICP HTLSFRPVVL EDSIDIRVCV PFDSRTTAWT SFDGRSRLEL KQGDHIKVTA SRYPFPIILY ADKSFPDWAS SLSRKLRWNE RERQKPYVLV EEARQQK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccag gaagaaggat tgtgcgctct cgaatacagc atgttttgat cgtcactaaa gtgaatgaca atcaacttgt ctctcttaca agggagctgg ctcttcatct catgcaaaag agcccttcga tacccaaagg aaccaacagt cgttatggca cagcatcact tgggagaggc atggtggtct atgtagactc tgagtttgag acttcaaagg cgttcgatgt cccgggcttg cagcgagatc atccggagct ttttgagccc atgattagca atcgctcttc gtcctctctc tcgtccatga acactctaaa tgagtcttct acagacgagg agccagtcac ctcggggtca tcgaccccag tcggtgaggg acagctgagg tactggacca acaagctttg ttcaaacact cctcacttat ttgacctcgt cataacactc ggaggtgatg ggacagtcct atatacttct tggcttttcc aacgaattgt tcctcctgtc cttccctttg cccttggctc cctcggtttc ttgaccaaat tcgatttcaa ggactacaaa gagataattg acaaggttat tcgtgatggg atacgagtca gccttcgtat gcgtttttgc tgtacagtat atcgtacgag cactcctggc aatattgacg gccccaaggc caaaaagcgg cgaatcatca aggacggcag cgccagtgcc cttaagaaaa gggttcataa gagtggctgg gagagcttgg aagatgaaga agcagattca catttgtctg atgctgggag tgacgaagat gtaatcatgc gtcattcaac aaggcccgaa gaacagtttg aagtcctcaa tgagctagta gtggatagag gtcctaattc agccatgagc tccttggagc tatttggtga cgaatatcat ttgacaactg tacaagcaga cgggctcacg gtttcaacac ccactggttc tacggcctac tccttatcag ccggtggatc actcacctcc cctcaaacat ctaacatcct cataactcca atctgtcctc acacgctttc cttcagacct gtcgtcttgg aggatagcat cgatattcgg gtttgtgtgc ccttcgattc gaggacaacg gcttggacca gctttgatgg aaggagtcgc ctggagttga aacagggaga tcatattaaa gttacagcct cgagatatcc gttcccaatt atattgtatg ccgacaaatc cttcccagac tgggcatctt cgctctcacg aaagttgcga tggaacgaaa gggagagaca gaagccatat gtgcttgttg aagaagcccg tcagcagaaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for CrneC.19334.a.B1.PS38301
PURIFICATION DATe: 6/14/2017
CONCENTRATION: 10.8mg/ml
OBSERVED MW: 51kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer: 20mM HEPES, 300mM NaCl, 5% glycerol, 1mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP GRRIVRSRIQ HVLIVTKVND NQLVSLTREL ALHLMQKSPS IPKGTNSRYG TASLGRGMVV YVDSEFETSK AFDVPGLQRD HPELFEPMIS NRSSSSLSSM NTLNESSTDE EPVTSGSSTP VGEGQLRYWT NKLCSNTPHL FDLVITLGGD GTVLYTSWLF QRIVPPVLPF ALGSLGFLTK FDFKDYKEII DKVIRDGIRV SLRMRFCCTV YRTSTPGNID GPKAKKRRII KDGSASALKK RVHKSGWESL EDEEADSHLS DAGSDEDVIM RHSTRPEEQF EVLNELVVDR GPNSAMSSLE LFGDEYHLTT VQADGLTVST PTGSTAYSLS AGGSLTSPQT SNILITPICP HTLSFRPVVL EDSIDIRVCV PFDSRTTAWT SFDGRSRLEL KQGDHIKVTA SRYPFPIILY ADKSFPDWAS SLSRKLRWNE RERQKPYV
Validated NT Sequence
agcacatatg gcttctgtct ctccctttcg ttccatcgca actttcgtga gagcgaagat gcccagtctg ggaaggattt gtcggcatac aatataattg ggaacggata tctcgaggct gtaactttaa tatgatctcc ctgtttcaac tccaggcgac tccttccatc aaagctggtc caagccgttg tcctcgaatc gaagggcaca caaacccgaa tatcgatgct atcctccaag acgacaggtc tgaaggaaag cgtgtgagga cagattggag ttatgaggat gttagatgtt tgaggggagg tgagtgatcc accggctgat aaggagtagg ccgtagaacc agtgggtgtt gaaaccgtga gcccgtctgc ttgtacagtt gtcaaatgat attcgtcacc aaatagctcc aaggagctca tggctgaatt aggacctcta tccactacta gctcattgag gacttcaaac tgttcttcgg gccttgttga atgacgcatg attacatctt cgtcactccc agcatcagac aaatgtgaat ctgcttcttc atcttccaag ctctcccagc cactcttatg aacccttttc ttaagggcac tggcgctgcc gtccttgatg attcgccgct ttttggcctt ggggccgtca atattgccag gagtgctcgt acgatatact gtacagcaaa aacgcatacg aaggctgact cgtatcccat cacgaataac cttgtcaatt atctctttgt agtccttgaa atcgaatttg gtcaagaaac cgagggagcc aagggcaaag ggaaggacag gaggaacaat tcgttggaaa agccaagaag tatataggac tgtcccatca cctccgagtg ttatgacgag gtcaaataag tgaggagtgt ttgaacaaag cttgttggtc cagtacctca gctgtccctc accgactggg gtcgatgacc ccgaggtgac tggctcctcg tctgtagaag actcatttag agtgttcatg gacgagagag aggacgaaga gcgattgcta atcatgggct caaaaagctc cggatgatct cgctgcaagc ccgggacatc gaacgccttt gaagtctcaa actcagagtc tacatagacc accatgcctc tcccaagtga tgctgtgcca taacgactgt tggttccttt gggtatcgaa gggctctttt gcatgagatg aagagccagc tcccttgtaa gagagacaag ttgattgtca ttcactttag tgacgatcaa aacatgctgt attcgagagc gcacaatcct tcttcctggc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMP GRRIVRSRIQ HVLIVTKVND NQLVSLTREL ALHLMQKSPS IPKGTNSRYG TASLGRGMVV YVDSEFETSK AFDVPGLQRD HPELFEPMIS NRSSSSLSSM NTLNESSTDE EPVTSGSSTP VGEGQLRYWT NKLCSNTPHL FDLVITLGGD GTVLYTSWLF QRIVPPVLPF ALGSLGFLTK FDFKDYKEII DKVIRDGIRV SLRMRFCCTV YRTSTPGNID GPKAKKRRII KDGSASALKK RVHKSGWESL EDEEADSHLS DAGSDEDVIM RHSTRPEEQF EVLNELVVDR GPNSAMSSLE LFGDEYHLTT VQADGLTVST PTGSTAYSLS AGGSLTSPQT SNILITPICP HTLSFRPVVL EDSIDIRVCV PFDSRTTAWT SFDGRSRLEL KQGDHIKVTA SRYPFPIILY ADKSFPDWAS SLSRKLRWNE RERQKPYVLV EEARQQK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCAG GAAGAAGGAT TGTGCGCTCT CGAATACAGC ATGTTTTGAT CGTCACTAAA GTGAATGACA ATCAACTTGT CTCTCTTACA AGGGAGCTGG CTCTTCATCT CATGCAAAAG AGCCCTTCGA TACCCAAAGG AACCAACAGT CGTTATGGCA CAGCATCACT TGGGAGAGGC ATGGTGGTCT ATGTAGACTC TGAGTTTGAG ACTTCAAAGG CGTTCGATGT CCCGGGCTTG CAGCGAGATC ATCCGGAGCT TTTTGAGCCC ATGATTAGCA ATCGCTCTTC GTCCTCTCTC TCGTCCATGA ACACTCTAAA TGAGTCTTCT ACAGACGAGG AGCCAGTCAC CTCGGGGTCA TCGACCCCAG TCGGTGAGGG ACAGCTGAGG TACTGGACCA ACAAGCTTTG TTCAAACACT CCTCACTTAT TTGACCTCGT CATAACACTC GGAGGTGATG GGACAGTCCT ATATACTTCT TGGCTTTTCC AACGAATTGT TCCTCCTGTC CTTCCCTTTG CCCTTGGCTC CCTCGGTTTC TTGACCAAAT TCGATTTCAA GGACTACAAA GAGATAATTG ACAAGGTTAT TCGTGATGGG ATACGAGTCA GCCTTCGTAT GCGTTTTTGC TGTACAGTAT ATCGTACGAG CACTCCTGGC AATATTGACG GCCCCAAGGC CAAAAAGCGG CGAATCATCA AGGACGGCAG CGCCAGTGCC CTTAAGAAAA GGGTTCATAA GAGTGGCTGG GAGAGCTTGG AAGATGAAGA AGCAGATTCA CATTTGTCTG ATGCTGGGAG TGACGAAGAT GTAATCATGC GTCATTCAAC AAGGCCCGAA GAACAGTTTG AAGTCCTCAA TGAGCTAGTA GTGGATAGAG GTCCTAATTC AGCCATGAGC TCCTTGGAGC TATTTGGTGA CGAATATCAT TTGACAACTG TACAAGCAGA CGGGCTCACG GTTTCAACAC CCACTGGTTC TACGGCCTAC TCCTTATCAG CCGGTGGATC ACTCACCTCC CCTCAAACAT CTAACATCCT CATAACTCCA ATCTGTCCTC ACACGCTTTC CTTCAGACCT GTCGTCTTGG AGGATAGCAT CGATATTCGG GTTTGTGTGC CCTTCGATTC GAGGACAACG GCTTGGACCA GCTTTGATGG AAGGAGTCGC CTGGAGTTGA AACAGGGAGA TCATATTAAA GTTACAGCCT CGAGATATCC GTTCCCAATT ATATTGTATG CCGACAAATC CTTCCCAGAC TGGGCATCTT CGCTCTCACG AAAGTTGCGA TGGAACGAAA GGGAGAGACA GAAGCCATAT GTGCTTGTTG AAGAAGCCCG TCAGCAGAAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT