SSGCID: Target Page

CrneC.19333.a

Atypical/PI4K protein kinase

CENTER ID:	CrneC.19333.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19333.a.B2.GE40924	kinase domain truncation 1		935	1250
CrneC.19333.a.B3.GE40925	kinase domain truncation 2		890	1246

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_07744
UniProt:	J9VYF4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSHALLLRLF LSPYFSISIA MQYLKTYPDS IGISHYLCWR MKRMPAEEVE FYWPQICHLL LTYPTQSNAL ESFIIARAEE STHSAMLTFW FMQAALRDLI PTRQTDPVPF AICQRVLHRC HAIIFGDPPE PSRSPYRSLP HSPSSSALGK LSSIPGSGSS STFIQIDEGQ RQGQGLGQGR VNPHAAAALV GMGVMFAGIP GMPALVPLVG EWALIQGRRP MDDLTSGRDR VEVDTGGGAD VRPEKMERRR KENENERGEK EDSESEEEDG RAPSNGIIQG QGYKSNVKFP KSTVNLAITG PAQTTPNLYS PPRSPSAVPP FSAIDMPDEK VREKEKNGGI SSRRKGSDPF GQLFNDRSPT PPKSAHQPFH SVPSLPRSSS YNPNHYQGGP PTAEAILATY SFSAQRQLLT SHYCRSEVRF LLLLEDISNR LLVIPKPARV SALRAELTSL NHNLPAEVCM PLWCTADHTL SPSSSSSTLS PSPASGSNNP SSSAKNRAYH PRGSAVPGGH SRVVRISPGD SVVLNSAERA PYLLHVEILQ GDLNFDPTRR ENRELLKKIV VAEDRKRRKR DGTLGVGVDG REAGSGGRVR NAGYNNGRQE WGLGSKKGKG KGEGFGSLMP DSIPAIPPSP SPASASISNS SKPPSQPPLT ATTTTPGTPK PIPPEEMDLV EQLYGTLSVH DEIPDLSEII PLPSGPKNRD LDMKAWDRGS PVPGRITSSG VGVDAGAGIG AGVMGDVGQW MSSPTGTATG SEVGKSGPST PGFPSAKSGF TSPSPSSSSP FTPGLVQAQG QSPSTPGPKR AITLEDYSER MRTAAVMLAQ LNASLVPNAP ENQSGGGWIP GTGWIRTPGG GGGGGGEEAG GKPDGSAGGK LKLAAAQAAA IRERIMLEMM ALEEERVERM TDRPPGADVP IPTAETSGGG ESGHEHDHEH GTVEDEGIVR RELNKADPSA AVFRESWSAK KSRIRAGSPW GHLANWDVIS VIVKTGADLR QEQLATQLIE RFSRIWKEEN CDCWARFFRI LITGETSGLV ETVTDAVSVH SIKKGEYAKR LAEGGPIGHV SLMDHYVNTF GKPDSGRFAR AQRNFIRSLA GYSIITYLLQ IKDRHNGNIL VDQDGHLIHI DFGFMLSNSP GNMGFEAAPF KMLMEYVDIM GGLNSPGYAY FKKLFKEGFE AARKHSDSLI TIVELMQKNS KLDCFALFGD QTAAHFRERF QLGLTTQAVD AYLERLIVTS TGSNYTKLYD TFQYYSQGVL

NT Sequence

atgtctcacg cattactcct ccgtttgttc ctctccccat acttctccat ctccatcgcc atgcagtacc ttaagaccta tcccgactct attggtatat cccactatct ctgctggaga atgaagagga tgcccgccga agaagtcgag ttttattggc ctcagatatg ccatttgtta ttgacttacc ctactcaatc caatgctctc gagtcgttca tcatcgcccg agcagaggaa agcacccact ctgccatgct cacattctgg ttcatgcaag ctgctctccg ggacctcatc ccaacccggc agacggaccc tgtcccgttc gcaatctgcc aacgcgtcct gcaccgctgc catgccatca tctttggtga tcccccagaa ccgtcgcgct cgccttaccg ctctttaccg cattcgccat ctagttctgc cttgggcaag ctctcttcca tccccggctc tggttctagc tctaccttta ttcaaattga cgagggccaa aggcaagggc aaggattagg ccaggggaga gtgaacccgc atgcggctgc ggcgttggta ggtatgggtg tgatgttcgc tggtataccc ggtatgcccg ctctagtccc cctcgtaggc gaatgggctc tcatccaagg tcgccggcca atggacgatc taacaagtgg aagggatagg gtggaagtgg atacgggtgg tggagcggat gtgaggcctg aaaagatgga aaggaggaga aaggaaaatg agaatgagag gggggagaag gaggatagtg agtcggaaga ggaagatggg cgagcgccga gtaatgggat tatccaagga caaggataca aatcgaatgt taaattccct aagtctaccg tcaacctagc aatcactggc cccgctcaaa caacgcccaa cctctattct ccacctcgat ctccatctgc agttcctccc ttctccgcaa tcgacatgcc cgatgagaag gtaagggaaa aggaaaaaaa tggcggcatt tcttctcgcc gaaagggttc agacccattc ggtcaactgt tcaatgacag atcccctact ccaccgaaat ccgcgcacca acccttccat tccgtacctt ctttaccccg ttcatcgtct tataacccca accattacca gggaggacca cccacagcgg aagccatcct cgcgacctac tcgttctctg ctcaaagaca acttctcacg agtcactact gtaggagcga agtgaggttc ttgctattat tggaagatat cagtaataga ttactggtca ttcctaaacc ggcgcgggtt agtgctttga gagcagaatt gacgagtttg aaccacaacc tcccagcaga agtgtgcatg cctctatggt gtactgccga tcataccctt tctccttcct cctcctcatc caccttgagc ccatcacccg catctgggtc gaacaaccct tcttcctcgg caaaaaatcg agcataccac ccacgcggtt ctgccgtccc aggtggtcat tcacgggttg tacgtatctc cccgggcgat tccgtcgtcc tcaactcggc agaaagagca ccgtacctgt tacacgtcga gatcctccaa ggggatctca attttgaccc gacgagaagg gagaataggg aattgttgaa gaaaattgtg gtagcagagg atagaaaaag aagaaaaagg gatgggacat tgggtgtagg tgtagatggg cgtgaagctg gttctggtgg cagggtacgt aatgcagggt acaataatgg tcgacaagaa tggggattag ggagtaaaaa aggaaaaggg aaaggagaag gctttggaag tctcatgcct gattctattc ccgccatccc gccttcccct tcccctgcct cagcttcaat ctcaaactct tccaaaccac cttcccaacc acctttaacc gccaccacca ccacccctgg tacgcccaag cccatccccc cagaagaaat ggaccttgtc gaacaacttt acggtaccct ctccgttcac gacgagattc ccgatttgtc cgaaattatc cccttaccga gcgggcctaa gaatagggat ttggatatga aagcgtggga tcgggggtcg cctgtccctg gccggataac tagctcgggt gtgggtgtgg atgcgggtgc ggggataggt gcgggtgtga tgggtgatgt agggcagtgg atgtcttccc cgacgggaac ggcaacagga agcgaggtcg gcaaatccgg accgtctaca cctggcttcc catcagctaa atccggcttt acctcccctt ctccatcttc ctcgtctcct ttcactcctg gcttggttca agctcaaggt caaagtcctt ctactcccgg accaaaaagg gcaattactc tcgaagatta ctcggaacgt atgcgcacag cggccgtcat gcttgctcaa ttgaacgctt ccctcgtacc gaatgcacca gagaaccaaa gtgggggcgg gtggataccg ggtacagggt ggataaggac tccaggcggt ggtggaggtg gtggagggga agaggctggt gggaagccag atggttcggc gggaggcaaa ctgaagctag cggcagcaca agcggcagcg ataagagaac ggatcatgct cgagatgatg gctcttgaag aagaacgagt ggaacgtatg acagatcgac cgcctggtgc ggatgtaccc atccccacag ctgaaacgtc tggaggaggt gaaagcggac atgagcatga tcatgaacat ggtacggtgg aagatgaagg aattgtgagg agggaattga ataaagcaga tccatccgca gctgtattca gggaaagctg gtcagcgaag aagagcagaa ttagagctgg aagtccgtgg ggccatctgg caaattggga tgtcatctca gtaatcgtca agacaggtgc ggatctaagg caggagcaac ttgcaacgca gttgattgaa cggttcagcc gaatttggaa agaggaaaat tgtgattgtt gggccagatt cttccgaatc cttataaccg gtgaaacatc tggattggtg gaaaccgtga cggatgcggt gtctgtacat tctatcaaaa agggagaata tgccaagcga ttggctgaag gagggcctat tgggcatgtg tccttgatgg accactacgt caacacattc ggtaaaccag actctggtcg attcgctcgt gctcaacgca attttatccg ttctctcgca ggttactcta tcatcacata ccttcttcag atcaaagatc ggcataacgg gaacatcctc gtagaccagg atgggcattt gattcatatt gactttgggt tcatgttgag taactcgccg ggaaatatgg gatttgaggc ggcacctttc aagatgctta tggagtatgt ggatatcatg ggaggattga atagccctgg ttatgcgtac ttcaagaagc tgttcaagga aggattcgaa gctgcaagga aacattcaga tagtttgatc acaattgtcg agctcatgca gaagaactct aagctcgact gttttgctct ctttggtgac cagacggctg ctcatttccg agaacgtttc caactaggcc tcacgacgca agctgtagac gcatatttgg aacgcttaat tgtaacctca actgggagca attataccaa gttgtatgat actttccagt attacagtca aggtgtctta taa

Details for CrneC.19333.a.B2.GE40924

HARVESTED ON:	12/20/2016
SEQUENCED ON:	12/27/2016
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHHM DEGIVRRELN KADPSAAVFR ESWSAKKSRI RAGSPWGHLA NWDVISVIVK TGADLRQEQL ATQLIERFSR IWKEENCDCW ARFFRILITG ETSGLVETVT DAVSVHSIKK GEYAKRLAEG GPIGHVSLMD HYVNTFGKPD SGRFARAQRN FIRSLAGYSI ITYLLQIKDR HNGNILVDQD GHLIHIDFGF MLSNSPGNMG FEAAPFKMLM EYVDIMGGLN SPGYAYFKKL FKEGFEAARK HSDSLITIVE LMQKNSKLDC FALFGDQTAA HFRERFQLGL TTQAVDAYLE RLIVTSTGSN YTKLYD

Validated NT Sequence

agtatcatac aacttggtat aattgctccc agttgaggtt acaattaagc gttccaaata tgcgtctaca gcttgcgtcg tgaggcctag ttggaaacgt tctcggaaat gagcagccgt ctggtcacca aagagagcaa aacagtcgag cttagagttc ttctgcatga gctcgacaat tgtgatcaaa ctatctgaat gtttccttgc agcttcgaat ccttccttga acagcttctt gaagtacgca taaccagggc tattcaatcc tcccatgata tccacatact ccataagcat cttgaaaggt gccgcctcaa atcccatatt tcccggcgag ttactcaaca tgaacccaaa gtcaatatga atcaaatgcc catcctggtc tacgaggatg ttcccgttat gccgatcttt gatctgaaga aggtatgtga tgatagagta acctgcgaga gaacggataa aattgcgttg agcacgagcg aatcgaccag agtctggttt accgaatgtg ttgacgtagt ggtccatcaa ggacacatgc ccaataggcc ctccttcagc caatcgcttg gcatattctc cctttttgat agaatgtaca gacaccgcat ccgtcacggt ttccaccaat ccagatgttt caccggttat aaggattcgg aagaatctgg cccaacaatc acaattttcc tctttccaaa ttcggctgaa ccgttcaatc aactgcgttg caagttgctc ctgccttaga tccgcacctg tcttgacgat tactgagatg acatcccaat ttgccagatg gccccacgga cttccagctc taattctgct cttcttcgct gaccagcttt ccctgaatac agctgcggat ggatctgctt tattcaattc cctcctcaca attccttcat ccatatggtg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHDE GIVRRELNKA DPSAAVFRES WSAKKSRIRA GSPWGHLANW DVISVIVKTG ADLRQEQLAT QLIERFSRIW KEENCDCWAR FFRILITGET SGLVETVTDA VSVHSIKKGE YAKRLAEGGP IGHVSLMDHY VNTFGKPDSG RFARAQRNFI RSLAGYSIIT YLLQIKDRHN GNILVDQDGH LIHIDFGFML SNSPGNMGFE AAPFKMLMEY VDIMGGLNSP GYAYFKKLFK EGFEAARKHS DSLITIVELM QKNSKLDCFA LFGDQTAAHF RERFQLGLTT QAVDAYLERL IVTSTGSNYT KLYDTFQYYS QGVL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgatgaag gaattgtgag gagggaattg aataaagcag atccatccgc agctgtattc agggaaagct ggtcagcgaa gaagagcaga attagagctg gaagtccgtg gggccatctg gcaaattggg atgtcatctc agtaatcgtc aagacaggtg cggatctaag gcaggagcaa cttgcaacgc agttgattga acggttcagc cgaatttgga aagaggaaaa ttgtgattgt tgggccagat tcttccgaat ccttataacc ggtgaaacat ctggattggt ggaaaccgtg acggatgcgg tgtctgtaca ttctatcaaa aagggagaat atgccaagcg attggctgaa ggagggccta ttgggcatgt gtccttgatg gaccactacg tcaacacatt cggtaaacca gactctggtc gattcgctcg tgctcaacgc aattttatcc gttctctcgc aggttactct atcatcacat accttcttca gatcaaagat cggcataacg ggaacatcct cgtagaccag gatgggcatt tgattcatat tgactttggg ttcatgttga gtaactcgcc gggaaatatg ggatttgagg cggcaccttt caagatgctt atggagtatg tggatatcat gggaggattg aatagccctg gttatgcgta cttcaagaag ctgttcaagg aaggattcga agctgcaagg aaacattcag atagtttgat cacaattgtc gagctcatgc agaagaactc taagctcgac tgttttgctc tctttggtga ccagacggct gctcatttcc gagaacgttt ccaactaggc ctcacgacgc aagctgtaga cgcatatttg gaacgcttaa ttgtaacctc aactgggagc aattatacca agttgtatga tactttccag tattacagtc aaggtgtctt atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for CrneC.19333.a.B3.GE40925

HARVESTED ON:	data unavailable
SEQUENCED ON:	12/27/2016
EXPECTED MW:	41kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMA LEEERVERMT DRPPGADVPI PTAETSGGGE SGHEHDHEHG TVEDEGIVRR ELNKADPSAA VFRESWSAKK SRIRAGSPWG HLANWDVISV IVKTGADLRQ EQLATQLIER FSRIWKEENC DCWARFFRIL ITGETSGLVE TVTDAVSVHS IKKGEYAKRL AEGGPIGHVS LMDHYVNTFG KPDSGRFARA QRNFIRSLAG YSIITYLLQI KDRHNGNILV DQDGHLIHID FGFMLSNSPG NMGFEAAPFK MLMEYVDIMG GLNSPGYAYF KKLFKEGFEA ARKHSDSLIT IVELMQKNSK LDCFALFGDQ TAAHFRERFQ LGLTTQAVDA YLERLIVTST GSNYTKX

Validated NT Sequence

anaacttggt ataattgctc ccagttgagg ttacaattaa gcgttccaaa tatgcgtcta cagcttgcgt cgtgaggcct agttggaaac gttctcggaa atgagcagcc gtctggtcac caaagagagc aaaacagtcg agcttagagt tcttctgcat gagctcgaca attgtgatca aactatctga atgtttcctt gcagcttcga atccttcctt gaacagcttc ttgaagtacg cataaccagg gctattcaat cctcccatga tatccacata ctccataagc atcttgaaag gtgccgcctc aaatcccata tttcccggcg agttactcaa catgaaccca aagtcaatat gaatcaaatg cccatcctgg tctacgagga tgttcccgtt atgccgatct ttgatctgaa gaaggtatgt gatgatagag taacctgcga gagaacggat aaaattgcgt tgagcacgag cgaatcgacc agagtctggt ttaccgaatg tgttgacgta gtggtccatc aaggaaacat gcccaatagg ccctccttca gccaatcgct tggcatattc tccctttttg atagaatgta cagacaccgc atccgtcacg gtttccacca atccagatgt ttcaccggtt ataaggattc ggaagaatct ggcccaacaa tcacaatttt cctctttcca aattcggctg aaccgttcaa tcaactgcgt tgcaagttgc tcctgcctta gatccgcacc tgtcttgacg attactgaga tgacatccca atttgccaga tggccccacg gacttccagc tctaattctg ctcttcttcg ctgaccagct ttccctgaat acagctgcgg atggatctgc tttattcaat tccctcctca caattccttc atcttccacc gtaccatgtt catgatcatg ctcatgtccg ctttcacctc ctccagacgt ttcagctgtg gggatgggta catccgcacc aggcggtcga tctgtcatac gttccactcg ttcttcttca agagccatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctc ttgaagaaga acgagtggaa cgtatgacag atcgaccgcc tggtgcggat gtacccatcc ccacagctga aacgtctgga ggaggtgaaa gcggacatga gcatgatcat gaacatggta cggtggaaga tgaaggaatt gtgaggaggg aattgaataa agcagatcca tccgcagctg tattcaggga aagctggtca gcgaagaaga gcagaattag agctggaagt ccgtggggcc atctggcaaa ttgggatgtc atctcagtaa tcgtcaagac aggtgcggat ctaaggcagg agcaacttgc aacgcagttg attgaacggt tcagccgaat ttggaaagag gaaaattgtg attgttgggc cagattcttc cgaatcctta taaccggtga aacatctgga ttggtggaaa ccgtgacgga tgcggtgtct gtacattcta tcaaaaaggg agaatatgcc aagcgattgg ctgaaggagg gcctattggg catgtgtcct tgatggacca ctacgtcaac acattcggta aaccagactc tggtcgattc gctcgtgctc aacgcaattt tatccgttct ctcgcaggtt actctatcat cacatacctt cttcagatca aagatcggca taacgggaac atcctcgtag accaggatgg gcatttgatt catattgact ttgggttcat gttgagtaac tcgccgggaa atatgggatt tgaggcggca cctttcaaga tgcttatgga gtatgtggat atcatgggag gattgaatag ccctggttat gcgtacttca agaagctgtt caaggaagga ttcgaagctg caaggaaaca ttcagatagt ttgatcacaa ttgtcgagct catgcagaag aactctaagc tcgactgttt tgctctcttt ggtgaccaga cggctgctca tttccgagaa cgtttccaac taggcctcac gacgcaagct gtagacgcat atttggaacg cttaattgta acctcaactg ggagcaatta taccaagttg tatgatactt tccagtatta cagttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat