CrneC.19331.a

Phosphatidylinositol 4-kinase

CENTER ID: CrneC.19331.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19331.a.B2.GE40923 accessory PIK domain and kinase domain 1457 2017
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_04335
UniProt: J9VUI0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDSLDEPLHL HILTSLASSY AAHPSNSDEQ PINALTVGLP QTALSTSAGL DLLGSDEEDD EWTELEINHA ISYAHYVYSL PITSANLASS VGKLKKLLWG LIRQGTIVVD PAASPASSSS ARANVGLAGS VGQAQLCQDL IKAMIWAGWS SEDARSDVGE TLVRLGQQIA DLCASNAQTT FPLVLLNSVH SVITHCPLPP FPTSIIARLV STFLPISSPK FLVKLVRQSS SAASTPHFRL PKNSPFLAQY PSSPVAVVML VTEAITALLG NSLFSQPDID EVQKAFVKHS DQMNSSFLPN PEPPGSRPVL EHQISQLVSF PVEGHSLSLT SEGRAVLAEV DRMSLRWWSD LMGSAYIDTE RELPGKRGNL FAHGRNEPEE EVDLTVAVLH LMNMIVLRQP DGDKTQLARL KMLLSESSTI TDARVLEAAF TCTTILVRNN PSLGTPMTHH IRRLLMTPLP AFEGEIAGLG GDVPPAVVAA GKCLSVCIEM SADDDLTSST LYSLLNTLSH GQGNSGSAGV NSIRSLPLHH QGGEADTYTL KTTLTGGKGS EEQKRLVAAA AVQVVSRLAL ELGKDDILHL SLAMLLQRLR GVDITTEATI VANLVPLALA SSNADLVEVY RAFSQIARSS HPEDPRRSSN AILAALTTLA KGMYKRLDCA DGYLVELLTL FADKGTQTQM ISMAAHAYDS KDREQLAQLR TEGNTRVAEM KSWLSALLIP ISTLLSHPHY HPDEAASPEL VTHFRNLWFT LVVFGLSGNV GKNNLNEHEW EALGVIAEKT PVLVLESSND FVESELEYNS TLRKDFAASI QHRQSTALNE YLPQQRQSYD TRNLSTPQIT FLMAVHDLEE MRTLRHRPSV VLQYFCNVSL NTSSLIGCLD AIVQKVSKSF LRQLYHQAIT HSLPPIISKE VCKILIACTH RMSKVREKAL AYARLILETF SALLCDKEVV FTLLEILTLM RRACETQYTD EYSPVYEFSS DRMDLTLHLT DDYAVRNEII TQLHTVTCTW LTLAISRAPI EVQSILQSYL NEARDVLLID SVEMGAGLAL HFSKAISRLD RQETIMPNIG GWPSDSSNLV ASQFASKNYH TGELSGARLV LSYGLSDLQK GSPTESSQAE LVAFKSQMAQ AVSNIRINSK SLDVPEIRRL LLRGVSVLVA SRRMDRDILH YLVELPMVAF TPLAIAAGVD AWTWLLRQRP EAEVAIVGAI TAGWMQTIIE KKGFFNNSMN YQDPFERPVE YSPSDKKVTD LELAKARRLL RPHTLLVQVL SSQFHAIKYR ERAMTISLIR LMMRSLEAHR EMSTHPLARE VRFSFLLLGF QILSSSRMEA LLELRLRDNL YKAAFSWFSV RPQWSYGSDR IQVGAEIKLL QDFLSAVAND GIRAEHYTSS MADRTPAFLI PGASSIQEYL VQHKDRVRLL QLLVENEISR LAVWSNPVNE HGRTSAPAVG SLERSTSTAD WTRLLQMAWK LNPAMAVQMG ERFKIAPLQA EITHLVKSDP KAVQDVAEAL HFLLGDKLEQ DAKAALKWLP VWAAVPPVTA IVYFQPRYGN HPLILQYAMR VLEQHPVELT FFFVPQVVQA LRADGLGYVE RFIFETSKIS QLFCHQIIWN MKANTYRDDD ASQPDPMKPL LDRMIDMIVH GLSGKAKAFY DLEFDFFDEV TSISGKLKPF IKKSKPEKKA KIDEEMAKIK LSVGVYLPSN PDGVVVDLDR KSGRPLQSHA KAPFMATFKV QKEKLDLPSN QSIENADEAK TVKTKYDVWQ AAIFKVGDDC RQDVLALQII AMFKNVFTSI GLTLYLYPYR VTATAPGCGV IDVVPNATSR DEMGRAKIND LFSYFVDKYG GVDTASFQKA RLNFIQSMAA YSVACYILQI KDRHNGNIMI DGEGHIVHID FGFLFDIGPG GMKFEPSSFK LNHEMVALMG GRDSQGYKMF TELTVKAFLA IRPHADQLID AVHLMLGTAL PSFKGEGTIT RLRNRFQLQL GERQAAEFMM GVIRNAHENM RSNVYDGFQK MQNGIPY
NT Sequence
atggactccc ttgatgagcc gctccatctt cacatcctca cttcccttgc ttccagctac gctgcacacc cttcaaactc cgatgaacaa cccataaacg cactcacggt cggtcttcca cagaccgcgc tctccacatc ggctggccta gaccttctgg gatcagacga agaagacgat gaatggaccg agttggagat aaatcatgcg atatcttatg cccattatgt ctactcgctc cccatcacgt ctgcgaacct tgcatcttcg gttgggaaat tgaaaaagct tctgtgggga ctcattcgac aaggaacaat agtagtcgat cccgcagctt ctccggcgtc atcgtcaagt gcccgagcga acgtcggcct tgcgggttcg gtagggcaag cgcaactctg ccaagacctt atcaaagcta tgatttgggc cggctggagc agtgaggatg ctaggtctga tgtaggggaa acattggtga gacttgggca gcagattgca gatttgtgcg cgtcaaatgc gcaaaccacc tttcccttgg ttcttctcaa ctcggtccat tcggtgatca cccattgtcc ccttcctccg ttcccaacat ccatcattgc tcgattggta tccactttcc tccccatttc atccccgaaa ttccttgtga aactcgtcag gcaatcgtca tcagctgcct caacccctca ttttcgtttg cctaagaata gcccattttt agctcagtac ccgagttctc cagttgcggt ggttatgctt gtcacagagg caataactgc tctcttgggt aactccctct tttcccagcc tgacattgat gaagtccaaa aggcttttgt gaaacactct gatcaaatga attcctcgtt cctccctaat ccggaacctc ctggctcaag gcctgtattg gaacatcaaa ttagccaact tgtgtcattc ccagtggaag gtcacagctt atctttgaca tcggaaggcc gagcggtact tgcggaggtg gataggatga gtttgcgatg gtggagtgat ttgatgggat cagcgtacat tgatacagaa agagagcttc ctgggaagag gggtaatctg ttcgctcatg ggagaaatga gccggaggaa gaagtagatt tgactgttgc tgtccttcac ctgatgaaca tgatcgtctt gcgtcagcca gatggagaca aaactcagct tgccaggctt aaaatgttgc tctcagagag ttctacaatc acggatgcca gagttctcga ggctgccttc acttgcacca ccatccttgt caggaacaat ccttctcttg ggaccccaat gactcaccat atccgtcgtc tattgatgac ccctctgcca gcgttcgagg gggagatcgc ggggctgggc ggagatgtgc cccctgctgt agtggcagca ggaaagtgtc tgtccgtttg cattgagatg tccgccgacg atgaccttac ctcgtctacc ctttattccc ttcttaatac actcagccat gggcaaggaa attctggttc ggcaggcgtc aactccatcc gttctcttcc cttgcatcat caaggcggag aagctgatac gtacactttg aagaccactc tcacaggcgg gaaaggttca gaggagcaaa aaagacttgt agcggccgcc gcggttcagg ttgtcagtcg actggcgttg gagctaggga aggacgatat attgcatctc agtttagcta tgttgctaca gcggctccgg ggcgtcgaca tcaccacaga agcaactatt gttgccaatc ttgtacccct tgcacttgcc agctccaacg cagacctcgt cgaagtctac cgcgcctttt cccaaatcgc tcgctcctct catcctgaag atccgcgcag atcgtccaac gccatcctcg ccgcattgac cacgctagcg aaaggcatgt acaagaggct cgactgcgca gatgggtatc tggtcgaact ccttacgctc tttgccgaca agggtactca aacccagatg atatccatgg ctgctcacgc atacgattct aaagaccgag aacagctcgc ccaactccgt acggaaggaa acactagagt tgcggaaatg aagtcttggc tttcggctct tcttatcccc atttcaactc tcttatccca tcctcattat catcccgatg aagcagcaag tcccgagctg gtaacacatt tcagaaacct ttggtttact ttggtggtct tcgggcttag cgggaatgtt ggaaagaata atttgaatga gcacgaatgg gaagcgctcg gggtcatcgc agaaaagaca ccagtcttgg tattggaaag ctcaaatgac tttgtggaga gtgagttgga gtataattcg acattgagaa aggactttgc tgcatctatt caacatcggc agtctacagc tttgaacgaa tacttacctc aacagcgcca aagctatgat acgcgcaact tgagcacgcc tcagatcact ttcctcatgg cggtgcatga cctcgaagaa atgaggacgc tccggcaccg accttctgtc gtcttgcaat atttttgcaa cgtcagtctg aacacaagct cgttgatcgg atgcctggac gcgattgtgc aaaaagtttc caaaagcttc ctgagacaac tttaccacca agccatcacc cattcgctcc ctcccataat cagcaaggag gtttgtaaaa ttcttatagc ttgcacgcat cgcatgagca aagtccgtga aaaggcgttg gcatacgctc gtctgatctt ggaaacgttc tctgcgttgt tatgcgataa agaggtggtg ttcactttac tggaaatctt gacgctcatg agacgcgcct gcgagactca gtacacagac gaatactccc ccgtatacga gttttcttcc gacaggatgg atctcaccct tcacctcact gacgattacg ctgttcgcaa tgagataatc actcaacttc acaccgtcac ctgcacttgg cttactcttg ccatatctcg cgcacccatc gaagtacaat ctatcctcca gagttatctg aacgaggcaa gagatgtgct gttgatcgac agcgtcgaga tgggggccgg attggcgctg catttctcga aggcaatatc tagacttgac agacaagaga ctatcatgcc taacataggt ggatggcctt ctgatagttc aaacctggtt gcaagtcagt ttgcttccaa aaattaccac actggagagc ttagtggcgc tcgattggtt ctttcttatg ggttaagtga tttacaaaaa gggtctccta ccgaatcctc acaagctgag ctggtagcat ttaaatccca aatggctcag gcagtgtcaa acatacgaat caacagcaaa tcccttgatg tccccgagat cagacgatta ttgctccgcg gggtctctgt ccttgtcgcg tctcgcagaa tggatcgaga catactccat tacttggtgg agcttcccat ggtggcattc acgcctttgg ccatcgccgc tggtgttgat gcttggacgt ggttgctccg acagcgtcca gaagctgaag tagctattgt tggtgcgatt actgccggat ggatgcagac gatcattgag aagaaaggat tttttaacaa ttctatgaac taccaagacc ccttcgagcg cccagtagag tattctccca gtgacaagaa ggtaacagac ctggaactcg ccaaggcacg ccgacttctt cgcccccata ctcttctcgt acaagtcttg tcctctcaat tccatgccat caagtatcgc gaaagagcga tgaccatctc cttgattagg ttgatgatgc gatcactgga agctcacagg gagatgagta ctcatccatt ggcaagagaa gtccgttttt ctttcttgct tttggggttt caaatacttt ccagtagtcg gatggaagcc ttgctggagc ttaggctgcg cgacaacctt tacaaagcag ccttctcctg gttctcagtc aggcctcagt ggtcgtatgg tagcgacagg attcaagtcg gggcggaaat caaacttttg caagactttc tttccgcagt agcaaacgat ggtatccgag ctgaacatta cacttcttcg atggccgatc gcactcccgc ctttctcata cctggagctt cttcaattca agaatattta gtccagcaca aagatcgagt gagacttctc cagcttttgg ttgaaaacga aatttcacga cttgccgtat ggagcaaccc ggtgaatgaa cacggaagga cttcggcgcc tgctgtgggt tcgcttgaaa ggtctacttc tacagccgat tggactcgct tgttgcaaat ggcttggaag cttaatcctg ccatggcagt ccaaatggga gaaagattta agatcgctcc tctacaggcg gaaatcactc atcttgtgaa atctgatccc aaagctgttc aagatgttgc tgaggcgctc cacttcctcc ttggcgataa gcttgaacag gatgcaaagg cagctcttaa atggttgcct gtatgggcag ctgtaccgcc agtcacagct atcgtgtatt tccaaccccg ctatggtaat catcctctta ttttacaata cgccatgcga gtattggaac agcatcccgt tgaattgacc tttttcttcg tgcctcaagt cgttcaagca cttcgtgctg acggcttggg atatgtggag agattcatct ttgagacgtc taagatttca cagctgtttt gccatcagat catctggaac atgaaagcca acacatatag ggatgacgat gctagccagc cggacccgat gaagcccctt ttggaccgca tgatagacat gattgttcat ggtctatctg ggaaagccaa agcgttctac gaccttgaat ttgatttctt cgacgaagta acttccatat caggtaaact caagccattc attaagaaat cgaagccgga gaagaaggcc aagatcgatg aagagatggc aaagatcaaa ctgtccgtgg gagtatactt gcctagcaat cccgacggtg ttgtcgtaga ccttgacagg aagtcaggtc ggcctcttca aagtcacgca aaagcacctt tcatggcgac atttaaagtc caaaaagaga agctcgacct accctccaat cagtccattg agaatgcaga tgaggccaaa accgtcaaaa ccaaatacga tgtctggcag gcggcgatct tcaaggtggg agatgactgt aggcaggatg tgctagcatt acagatcatt gcaatgttca agaatgtctt tacatccatt ggtctgacgc tttatctgta cccttaccga gtcaccgcga ctgcccctgg ctgcggtgtt atcgatgtcg tccccaatgc tacttcacgg gatgagatgg gacgggcaaa aataaacgac ttgttctctt atttcgttga caaatatggc ggtgtagata ctgcttcatt ccagaaggcg cgactcaatt tcatccagtc aatggccgca tattctgtag cttgctacat cttgcagatc aaggataggc acaacggtaa catcatgata gacggagagg gtcatattgt gcatatcgat tttggattct tgttcgatat tggcccgggt ggcatgaagt tcgaaccatc aagcttcaag cttaaccatg aaatggtggc tcttatgggc ggccgcgatt ctcaaggcta caaaatgttt actgagctta ccgtcaaggc cttcctcgct ataagacccc atgctgatca gcttattgat gctgtgcacc ttatgcttgg tactgcattg ccatcattca agggcgaagg cactattacc aggctacgga accgattcca gttacagctg ggggaaaggc aagcggcaga atttatgatg ggagtaatca gaaatgcgca tgaaaacatg cgaagcaacg tatacgatgg gttccagaaa atgcaaaacg gtattccata ctag
Details for CrneC.19331.a.B2.GE40923
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 64kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 76
PERCENT COVERAGE: 49
Validated AA Sequence
MAHHHHHHMS TADWTRLLQM AWKLNPAMAV QMGERFKIAP LQAEITHLVK SDPKAVQDVA EALHFLLGDK LEQDAKAALK WLPVWAAVPP VTAIVYFQPR YGNHPLILQY AMRVLEQHPV ELTFFFVPQV VQALRADGLG YVERFIFETS KISQLFCHQI IWNMKANTYR DDDASQPDPM KPLLDRMIDM IVHGLSGKAK XFXXLEFDFX DXVXXXSXXX XPFXXXXXXX XXXXXDXXXA XXQTXXXXXL AXXXXXXXXX XXXXXXXAXX XXXKXXXXXD I
Validated NT Sequence
tttctggaac ccatcgtata cgttgcttcg catgttttca tgcgcatttc tgattactcc catcataaat tctgccgctt gcctttcccc cagctgtaac tggaatcggt tccgtagcct ggtaatagtg ccttcgccct tgaatgatgg caatgcagta ccaagcataa ggtgcacagc atcaataagc tgatcagcat ggggtcttat agcgaggaag gccttgacgg taagctcagt aaacattttg tagccttgag aatcgcggcc gcccataaga gccaccattt catggttaag cttgaagctt gatggttcga acttcatgcc acccgggcca atatcgaaca agaatccaaa atcgatatgc acaatatgac cctctccgtc tatcatgatg ttaccgttgt gcctatcctt gatctgcaag atgtagcaag ctacagaata tgcggccatt gactggatga aattgagtcg cgccttctgg aatgaagcag tatctacacc gccatatttg tcaacgaaat aagagaccaa gtcgtttatt tttgcccgtc ccatctcatc ccgtgaagta gcattgggga cgacatcgat aacaccgcag ccaggggcag tcgcggtgac tcggtaaggg tacagataaa gcgtcagacc aatggatgta aagacattct tgaacattgc aatgatctgt aatgctagca catcctgcct acagtcatct cccaccttga agatcgccgc ctgcnnnnna tcgtatttgg ttttgacnnn nnnngcctca tctgcattct cnnnggactg attggaggnn nnnnngagnn nnnnnnnngn nactttaaat gtcgnnnnnn nnnnnnnntt tnnnnnnnnn nnnnnnngcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaggc aagtnnnnnn nnnnnnnnag tttgnnnntn tgccannnnn ncatcgannn nnnnnnnnnn nncnnnnnnn nnnnnnnnnn ngaatggnnn nnnnnnnnnn gatnnnnnan nnacnnngtc naagaaatca aattcaaggn nntagaacnn tttggctttc ccagatagac catgaacaat catgtctatc atgcggtcca aaaggggctt catcgggtcc ggctggctag catcgtcatc cctatatgtg ttggctttca tgttccagat gatctgatgg caaaacagct gtgaaatctt agacgtctca aagatgaatc tctccacata tcccaagccg tcagcacgaa gtgcttgaac gacttgaggc acgaagaaaa aggtcaattc aacgggatgc tgttccaata ctcgcatggc gtattgtaaa ataagaggat gattaccata gcggggttgg aaatacacga tagctgtgac tggcggtaca gctgcccata caggcaacca tttaagagct gcctttgcat cctgttcaag cttatcgcca aggaggaagt ggagcgcctc agcaacatct tgaacagctt tgggatcaga tttcacaaga tgagtgattt ccgcctgtag aggagcgatc ttaaatcttt ctcccatttg gactgccatg gcaggattaa gcttccaagc catttgcaac aagcgagtcc aatcggctgt agacatatgg tggtggtggt ggtgagccat ggtatat
Expected Protein Sequence
MAHHHHHHST ADWTRLLQMA WKLNPAMAVQ MGERFKIAPL QAEITHLVKS DPKAVQDVAE ALHFLLGDKL EQDAKAALKW LPVWAAVPPV TAIVYFQPRY GNHPLILQYA MRVLEQHPVE LTFFFVPQVV QALRADGLGY VERFIFETSK ISQLFCHQII WNMKANTYRD DDASQPDPMK PLLDRMIDMI VHGLSGKAKA FYDLEFDFFD EVTSISGKLK PFIKKSKPEK KAKIDEEMAK IKLSVGVYLP SNPDGVVVDL DRKSGRPLQS HAKAPFMATF KVQKEKLDLP SNQSIENADE AKTVKTKYDV WQAAIFKVGD DCRQDVLALQ IIAMFKNVFT SIGLTLYLYP YRVTATAPGC GVIDVVPNAT SRDEMGRAKI NDLFSYFVDK YGGVDTASFQ KARLNFIQSM AAYSVACYIL QIKDRHNGNI MIDGEGHIVH IDFGFLFDIG PGGMKFEPSS FKLNHEMVAL MGGRDSQGYK MFTELTVKAF LAIRPHADQL IDAVHLMLGT ALPSFKGEGT ITRLRNRFQL QLGERQAAEF MMGVIRNAHE NMRSNVYDGF QKMQNGIPY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctacag ccgattggac tcgcttgttg caaatggctt ggaagcttaa tcctgccatg gcagtccaaa tgggagaaag atttaagatc gctcctctac aggcggaaat cactcatctt gtgaaatctg atcccaaagc tgttcaagat gttgctgagg cgctccactt cctccttggc gataagcttg aacaggatgc aaaggcagct cttaaatggt tgcctgtatg ggcagctgta ccgccagtca cagctatcgt gtatttccaa ccccgctatg gtaatcatcc tcttatttta caatacgcca tgcgagtatt ggaacagcat cccgttgaat tgaccttttt cttcgtgcct caagtcgttc aagcacttcg tgctgacggc ttgggatatg tggagagatt catctttgag acgtctaaga tttcacagct gttttgccat cagatcatct ggaacatgaa agccaacaca tatagggatg acgatgctag ccagccggac ccgatgaagc cccttttgga ccgcatgata gacatgattg ttcatggtct atctgggaaa gccaaagcgt tctacgacct tgaatttgat ttcttcgacg aagtaacttc catatcaggt aaactcaagc cattcattaa gaaatcgaag ccggagaaga aggccaagat cgatgaagag atggcaaaga tcaaactgtc cgtgggagta tacttgccta gcaatcccga cggtgttgtc gtagaccttg acaggaagtc aggtcggcct cttcaaagtc acgcaaaagc acctttcatg gcgacattta aagtccaaaa agagaagctc gacctaccct ccaatcagtc cattgagaat gcagatgagg ccaaaaccgt caaaaccaaa tacgatgtct ggcaggcggc gatcttcaag gtgggagatg actgtaggca ggatgtgcta gcattacaga tcattgcaat gttcaagaat gtctttacat ccattggtct gacgctttat ctgtaccctt accgagtcac cgcgactgcc cctggctgcg gtgttatcga tgtcgtcccc aatgctactt cacgggatga gatgggacgg gcaaaaataa acgacttgtt ctcttatttc gttgacaaat atggcggtgt agatactgct tcattccaga aggcgcgact caatttcatc cagtcaatgg ccgcatattc tgtagcttgc tacatcttgc agatcaagga taggcacaac ggtaacatca tgatagacgg agagggtcat attgtgcata tcgattttgg attcttgttc gatattggcc cgggtggcat gaagttcgaa ccatcaagct tcaagcttaa ccatgaaatg gtggctctta tgggcggccg cgattctcaa ggctacaaaa tgtttactga gcttaccgtc aaggccttcc tcgctataag accccatgct gatcagctta ttgatgctgt gcaccttatg cttggtactg cattgccatc attcaagggc gaaggcacta ttaccaggct acggaaccga ttccagttac agctggggga aaggcaagcg gcagaattta tgatgggagt aatcagaaat gcgcatgaaa acatgcgaag caacgtatac gatgggttcc agaaaatgca aaacggtatt ccatactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t