CrneC.19330.a

Phosphomevalonate kinase

CENTER ID: CrneC.19330.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19330.a.B2.GE40922 GHMP kinase domain 229 531
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_06001
UniProt: J9VZX1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTRTTTVVS SPGKVLIAGG YLVLDTHYSG LVIGTSSRFY SCVSSRATSG TSDIDGNTDT DTCQDEAIIS VRAGQFPSDA STWVYSISKP SVSASASASA SASASAGEDG GDRNPYLKLE QTNEEQAGKN KFIFITLCKV LEYVYESILA KVGDEETALD ELIKRIKGGG FGEDGLEVVV FADNDFYSQR EQLTSLSLPT RISSLPHLPP FTPLPRPIPA TNKTGLGSSA ALVTSLVGSL LSHLCITHVS PEGGDISDDD KAVIHSVAQL AHCQAQGKVG SGFDVSSAVY GSHLYTRFSP SILTPLMSLA PFSRPRPSTA STSLLDALHP SKWDNKSIPF RLPKHVRLLL ADVSCGTDTP SFVSSVLKWR NNDREKADEV WERLDNANRA LGEVLRDMVD AEGESDYEKT MMAAAELTSN ELLNLHAAPS PSRTLHLLHR LALSLSSVRA LLREMSDLSG VPIEPKEQTR LLDACGQVKG VVGGGVPGAG GYDALYLLTV DHPTPLAGVD KLWADWTEMD VCPLSAKQSD GGTRQEEVAE VKGLKEALDR ARATG
NT Sequence
atgacgacaa ggacgacgac agtggtatcc tcccctggca aggtgcttat cgcgggagga taccttgtcc tcgacacgca ttactccggt ctagtaatcg gcacttctag tagattctac tcttgtgtct cgagccgcgc aacgtccggc acgtccgaca ttgacggtaa caccgacacc gacacttgtc aggatgaagc gatcatctcg gtacgcgccg gtcaattccc ttccgacgct tcgacgtggg tctactccat ctcaaaacct tctgtgtctg cgtctgcatc tgcgtctgca tctgcgtctg cgtcagccgg tgaagacggt ggagatcgga atccgtatct caagctcgag cagaccaacg aagagcaggc ggggaaaaac aagtttatct ttatcacgct ttgcaaagtg ctcgagtacg tttacgaatc catcttggcc aaagttgggg atgaggagac ggcattggac gagttgatca agcggataaa gggcggtgga tttggagagg atggtttgga ggtggtcgtc tttgcggaca atgactttta ctctcaacgc gagcagctca cgtcgctttc cctcccaacc cggatatcat cccttcccca tctcccgcca ttcacccctc ttccccgccc aatccctgca accaacaaaa ccggcctcgg ctcctctgcc gcgctagtca cttccctcgt cggatctttg ctctcccacc tttgtatcac gcatgtctct ccagagggcg gcgatatctc ggatgacgac aaagctgtca ttcactctgt tgctcagttg gcgcactgcc aagcgcaggg taaagttgga tcaggcttcg acgtttcctc cgctgtctac ggttcccacc tctacacccg tttctcccca tccatcctca cgcccctcat gtcccttgcc ccattctccc gtcctcggcc ctctaccgcc tctacatctc tcctcgatgc actccatccg agcaaatggg ataacaaatc catccctttc cgactcccca aacatgtgcg tctcttactc gccgacgttt cgtgcggtac cgacacgccc tcctttgtct cctccgtcct caagtggaga aataacgaca gggaaaaggc ggatgaagtg tgggaaaggt tggataatgc gaatagggcg ttgggagagg tgttgaggga tatggttgac gcagaaggcg agagtgatta tgaaaagacc atgatggccg ctgcagagct cacatcgaat gagcttctga acctgcatgc agcccctagc ccttcacgca cactccatct cctccatcgc ctcgccctct ccctctcgtc cgtccgcgct ctgttgcgtg aaatgtccga cctttccggc gtacctatcg aaccaaaaga acaaactaga ctcttagatg catgcgggca agtcaagggt gttgtgggtg gtggagttcc cggtgcgggc ggctacgatg ccctttacct ccttaccgtc gaccatccaa ctcctctagc aggtgtcgat aaactttggg cagattggac ggagatggac gtctgtccct tgtccgcaaa gcagagtgat ggtgggacaa ggcaagaaga agttgcggaa gtcaagggtt taaaggaagc gttggatagg gcgagggcga cgggataa
Details for CrneC.19330.a.B2.GE40922
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS AALVTSLVGS LLSHLCITHV SPEGGDISDD DKAVIHSVAQ LAHCQAQGKV GSGFDVSSAV YGSHLYTRFS PSILTPLMSL APFSRPRPST ASTSLLDALH PSKWDNKSIP FRLPKHVRLL LADVSCGTDT PSFVSSVLKW RNNDREKADE VWERLDNANR ALGEVLRDMV DAEGESDYEK TMMAAAELTS NELLNLHAAP SPSRTLHLLH RLALSLSSVR ALLREMSDLS GVPIEPKEQT RLLDACGQVK GVVGGGVPGA GGYDALYLLT VDHPTPLAGV DKLWADWTEM DVC
Validated NT Sequence
gggacagacg tccatctccg tccaatctgc ccaaagttta tcgacacctg ctagaggagt tggatggtcg acggtaagga ggtaaagggc atcgtagccg cccgcaccgg gaactccacc acccacaaca cccttgactt gcccgcatgc atctaagagt ctagtttgtt cttttggttc gataggtacg ccggaaaggt cggacatttc acgcaacaga gcgcggacgg acgagaggga gagggcgagg cgatggagga gatggagtgt gcgtgaaggg ctaggggctg catgcaggtt cagaagctca ttcgatgtga gctctgcagc ggccatcatg gtcttttcat aatcactctc gccttctgcg tcaaccatat ccctcaacac ctctcccaac gccctattcg cattatccaa cctttcccac acttcatccg ccttttccct gtcgttattt ctccacttga ggacggagga gacaaaggag ggcgtgtcgg taccgcacga aacgtcggcg agtaagagac gcacatgttt ggggagtcgg aaagggatgg atttgttatc ccatttgctc ggatggagtg catcgaggag agatgtagag gcggtagagg gccgaggacg ggagaatggg gcaagggaca tgaggggcgt gaggatggat ggggagaaac gggtgtagag gtgggaaccg tagacagcgg aggaaacgtc gaagcctgat ccaactttac cctgcgcttg gcagtgcgcc aactgagcaa cagagtgaat gacagctttg tcgtcatccg agatatcgcc gccctctgga gagacatgcg tgatacaaag gtgggagagc aaagatccga cgagggaagt gactagcgcg gcagacatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHSA ALVTSLVGSL LSHLCITHVS PEGGDISDDD KAVIHSVAQL AHCQAQGKVG SGFDVSSAVY GSHLYTRFSP SILTPLMSLA PFSRPRPSTA STSLLDALHP SKWDNKSIPF RLPKHVRLLL ADVSCGTDTP SFVSSVLKWR NNDREKADEV WERLDNANRA LGEVLRDMVD AEGESDYEKT MMAAAELTSN ELLNLHAAPS PSRTLHLLHR LALSLSSVRA LLREMSDLSG VPIEPKEQTR LLDACGQVKG VVGGGVPGAG GYDALYLLTV DHPTPLAGVD KLWADWTEMD VCPLSAKQSD G
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctgccg cgctagtcac ttccctcgtc ggatctttgc tctcccacct ttgtatcacg catgtctctc cagagggcgg cgatatctcg gatgacgaca aagctgtcat tcactctgtt gctcagttgg cgcactgcca agcgcagggt aaagttggat caggcttcga cgtttcctcc gctgtctacg gttcccacct ctacacccgt ttctccccat ccatcctcac gcccctcatg tcccttgccc cattctcccg tcctcggccc tctaccgcct ctacatctct cctcgatgca ctccatccga gcaaatggga taacaaatcc atccctttcc gactccccaa acatgtgcgt ctcttactcg ccgacgtttc gtgcggtacc gacacgccct cctttgtctc ctccgtcctc aagtggagaa ataacgacag ggaaaaggcg gatgaagtgt gggaaaggtt ggataatgcg aatagggcgt tgggagaggt gttgagggat atggttgacg cagaaggcga gagtgattat gaaaagacca tgatggccgc tgcagagctc acatcgaatg agcttctgaa cctgcatgca gcccctagcc cttcacgcac actccatctc ctccatcgcc tcgccctctc cctctcgtcc gtccgcgctc tgttgcgtga aatgtccgac ctttccggcg tacctatcga accaaaagaa caaactagac tcttagatgc atgcgggcaa gtcaagggtg ttgtgggtgg tggagttccc ggtgcgggcg gctacgatgc cctttacctc cttaccgtcg accatccaac tcctctagca ggtgtcgata aactttgggc agattggacg gagatggacg tctgtccctt gtccgcaaag cagagtgatg gttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat