CrneC.19325.a
D-glycerate 3-kinase
| CENTER ID: | CrneC.19325.a |
| ORGANISM: | Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487) |
| ASSOCIATED DISEASE: | Pneumonia-like illness |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrneC.19325.a.B1.GE40920 | full length | 1 | 322 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrneC.19325.a.B1.PW38244 | full length | 1 | 322 |
Structures
6CKG
DEPOSITED: 2/28/2018
DETERMINATION: XRay
CLONE: CrneC.19325.a.B1.GE40920
PROTEIN: CrneC.19325.a.B1.PW38244
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:CNAG_07779 |
| UniProt: | J9VS00 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTATAQIPGG AMVQQTAGFV LSQLARHRSS WNKETMCPPL VVGVQGPQGA GKSHLTGLLP DYLEKHYGLR LATMSLDDFY LTHSDQVKLS QSEPDNPLLN GRGPAGTHDL PLLEQCLAKL KSINDRSAKF PTATKDQRAQ LPIYDKSLFK GEGDRSKEVV EVQGPIDVVI FEGWMNGFGP LSNDKLEEKY AEAGRQWPSS SLVGAPGVMP TILLYSRSTL HSINQNLRQY EVLWDQIDCF VQIQPLDLSY VWTWRLQQEH NMKAKNGGNG MTDEQVRHFI NRYMPSYELF QDGIDKETTS WRGKGLRFIV NIKREIVGTE SF
NT Sequence
atgactgcta cagcacagat ccccggtgga gcaatggtcc agcagacagc cggtttcgtc ctatcacagc tcgctcgtca cagaagttca tggaataaag aaacgatgtg tcctcctctt gtagtaggtg tacagggccc tcaaggtgca ggcaaatcac acctaactgg tctattgcca gactatctgg aaaaacatta tggtctgcgt ctcgctacta tgtctttgga cgacttttac ctcacccatt ccgatcaggt caagctatcc caatccgagc ccgataaccc actcctcaac ggccgcggcc ccgccggtac ccacgattta ccacttctgg aacaatgtct ggcaaagttg aaatctatca atgaccgcag tgcgaagttt ccaactgcga ctaaggacca aagggctcag ctgcccatat atgacaaatc ccttttcaaa ggcgagggtg accggtcaaa ggaggtagta gaagtgcaag gaccgattga tgtggtcata tttgaagggt ggatgaatgg attcgggccg ttatcaaatg acaaactaga agagaaatac gccgaagccg gacgacaatg gccctcttct tctctcgtcg gcgcacctgg ggtcatgcct accattttat tgtattcgag atcaacgcta cacagtataa accaaaatct gaggcagtat gaagtacttt gggatcaaat tgattgtttt gtacagattc aaccgttgga tctgtcttac gtatggactt ggagacttca gcaagaacac aatatgaaag cgaagaatgg aggtaacgga atgactgatg agcaggtccg acatttcatt aaccgataca tgcccagcta tgagctgttt caagatggaa ttgacaagga gaccacatca tggaggggca aagggcttcg ttttattgtg aacataaaaa gagaaattgt tggcacagaa agcttctga
Details for CrneC.19325.a.B1.GE40920
| HARVESTED ON: | 12/20/2016 |
| SEQUENCED ON: | 12/27/2016 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMT ATAQIPGGAM VQQTAGFVLS QLARHRSSWN KETMCPPLVV GVQGPQGAGK SHLTGLLPDY LEKHYGLRLA TMSLDDFYLT HSDQVKLSQS EPDNPLLNGR GPAGTHDLPL LEQCLAKLKS INDRSAKFPT ATKDQRAQLP IYDKSLFKGE GDRSKEVVEV QGPIDVVIFE GWMNGFGPLS NDKLEEKYAE AGRQWPSSSL VGAPGVMPTI LLYSRSTLHS INQNLRQYEV LWDQIDCFVQ IQPLDLSYVW TWRLQQEHNM KAKNGGNGMT DEQVRHFINR YMPSYELFQD GIDKETTSWR GKGLRFIVNI KR
Validated NT Sequence
ttctcttttt atgttcacaa taaaacgaag ccctttgccc ctccatgatg tggtctcctt gtcaattcca tcttgaaaca gctcatagct gggcatgtat cggttaatga aatgtcggac ctgctcatca gtcattccgt tacctccatt cttcgctttc atattgtgtt cttgctgaag tctccaagtc catacgtaag acagatccaa cggttgaatc tgtacaaaac aatcaatttg atcccaaagt acttcatact gcctcagatt ttggtttata ctgtgtagcg ttgatctcga atacaataaa atggtaggca tgaccccagg tgcgccgacg agagaagaag agggccattg tcgtccggct tcggcgtatt tctcttctag tttgtcattt gataacggcc cgaatccatt catccaccct tcaaatatga ccacatcaat cggtccttgc acttctacta cctcctttga ccggtcaccc tcgcctttga aaagggattt gtcatatatg ggcagctgag ccctttggtc cttagtcgca gttggaaact tcgcactgcg gtcattgata gatttcaact ttgccagaca ttgttccaga agtggtaaat cgtgggtacc ggcggggccg cggccgttga ggagtgggtt atcgggctcg gattgggata gcttgacctg atcggaatgg gtgaggtaaa agtcgtccaa agacatagta gcgagacgca gaccataatg tttttccaga tagtctggca atagaccagt taggtgtgat ttgcctgcac cttgagggcc ctgtacacct actacaagag gaggacacat cgtttcttta ttccatgaac ttctgtgacg agcgagctgt gataggacga aaccggctgt ctgctggacc attgctccac cggggatctg tgctgtagca gtcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMT ATAQIPGGAM VQQTAGFVLS QLARHRSSWN KETMCPPLVV GVQGPQGAGK SHLTGLLPDY LEKHYGLRLA TMSLDDFYLT HSDQVKLSQS EPDNPLLNGR GPAGTHDLPL LEQCLAKLKS INDRSAKFPT ATKDQRAQLP IYDKSLFKGE GDRSKEVVEV QGPIDVVIFE GWMNGFGPLS NDKLEEKYAE AGRQWPSSSL VGAPGVMPTI LLYSRSTLHS INQNLRQYEV LWDQIDCFVQ IQPLDLSYVW TWRLQQEHNM KAKNGGNGMT DEQVRHFINR YMPSYELFQD GIDKETTSWR GKGLRFIVNI KREIVGTESF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg ctacagcaca gatccccggt ggagcaatgg tccagcagac agccggtttc gtcctatcac agctcgctcg tcacagaagt tcatggaata aagaaacgat gtgtcctcct cttgtagtag gtgtacaggg ccctcaaggt gcaggcaaat cacacctaac tggtctattg ccagactatc tggaaaaaca ttatggtctg cgtctcgcta ctatgtcttt ggacgacttt tacctcaccc attccgatca ggtcaagcta tcccaatccg agcccgataa cccactcctc aacggccgcg gccccgccgg tacccacgat ttaccacttc tggaacaatg tctggcaaag ttgaaatcta tcaatgaccg cagtgcgaag tttccaactg cgactaagga ccaaagggct cagctgccca tatatgacaa atcccttttc aaaggcgagg gtgaccggtc aaaggaggta gtagaagtgc aaggaccgat tgatgtggtc atatttgaag ggtggatgaa tggattcggg ccgttatcaa atgacaaact agaagagaaa tacgccgaag ccggacgaca atggccctct tcttctctcg tcggcgcacc tggggtcatg cctaccattt tattgtattc gagatcaacg ctacacagta taaaccaaaa tctgaggcag tatgaagtac tttgggatca aattgattgt tttgtacaga ttcaaccgtt ggatctgtct tacgtatgga cttggagact tcagcaagaa cacaatatga aagcgaagaa tggaggtaac ggaatgactg atgagcaggt ccgacatttc attaaccgat acatgcccag ctatgagctg tttcaagatg gaattgacaa ggagaccaca tcatggaggg gcaaagggct tcgttttatt gtgaacataa aaagagaaat tgttggcaca gaaagcttct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for CrneC.19325.a.B1.PW38244
| PURIFICATION DATe: | 4/10/2017 |
| CONCENTRATION: | 30mg/ml |
| OBSERVED MW: | 34kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT ATAQIPGGAM VQQTAGFVLS QLARHRSSWN KETMCPPLVV GVQGPQGAGK SHLTGLLPDY LEKHYGLRLA TMSLDDFYLT HSDQVKLSQS EPDNPLLNGR GPAGTHDLPL LEQCLAKLKS INDRSAKFPT ATKDQRAQLP IYDKSLFKGE GDRSKEVVEV QGPIDVVIFE GWMNGFGPLS NDKLEEKYAE AGRQWPSSSL VGAPGVMPTI LLYSRSTLHS INQNLRQYEV LWDQIDCFVQ IQPLDLSYVW TWRLQQEHNM KAKNGGNGMT DEQVRHFINR YMPSYELFQD GIDKETTSWR GKGLRFIVNI KR
Validated NT Sequence
ttctcttttt atgttcacaa taaaacgaag ccctttgccc ctccatgatg tggtctcctt gtcaattcca tcttgaaaca gctcatagct gggcatgtat cggttaatga aatgtcggac ctgctcatca gtcattccgt tacctccatt cttcgctttc atattgtgtt cttgctgaag tctccaagtc catacgtaag acagatccaa cggttgaatc tgtacaaaac aatcaatttg atcccaaagt acttcatact gcctcagatt ttggtttata ctgtgtagcg ttgatctcga atacaataaa atggtaggca tgaccccagg tgcgccgacg agagaagaag agggccattg tcgtccggct tcggcgtatt tctcttctag tttgtcattt gataacggcc cgaatccatt catccaccct tcaaatatga ccacatcaat cggtccttgc acttctacta cctcctttga ccggtcaccc tcgcctttga aaagggattt gtcatatatg ggcagctgag ccctttggtc cttagtcgca gttggaaact tcgcactgcg gtcattgata gatttcaact ttgccagaca ttgttccaga agtggtaaat cgtgggtacc ggcggggccg cggccgttga ggagtgggtt atcgggctcg gattgggata gcttgacctg atcggaatgg gtgaggtaaa agtcgtccaa agacatagta gcgagacgca gaccataatg tttttccaga tagtctggca atagaccagt taggtgtgat ttgcctgcac cttgagggcc ctgtacacct actacaagag gaggacacat cgtttcttta ttccatgaac ttctgtgacg agcgagctgt gataggacga aaccggctgt ctgctggacc attgctccac cggggatctg tgctgtagca gtcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMT ATAQIPGGAM VQQTAGFVLS QLARHRSSWN KETMCPPLVV GVQGPQGAGK SHLTGLLPDY LEKHYGLRLA TMSLDDFYLT HSDQVKLSQS EPDNPLLNGR GPAGTHDLPL LEQCLAKLKS INDRSAKFPT ATKDQRAQLP IYDKSLFKGE GDRSKEVVEV QGPIDVVIFE GWMNGFGPLS NDKLEEKYAE AGRQWPSSSL VGAPGVMPTI LLYSRSTLHS INQNLRQYEV LWDQIDCFVQ IQPLDLSYVW TWRLQQEHNM KAKNGGNGMT DEQVRHFINR YMPSYELFQD GIDKETTSWR GKGLRFIVNI KREIVGTESF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTG CTACAGCACA GATCCCCGGT GGAGCAATGG TCCAGCAGAC AGCCGGTTTC GTCCTATCAC AGCTCGCTCG TCACAGAAGT TCATGGAATA AAGAAACGAT GTGTCCTCCT CTTGTAGTAG GTGTACAGGG CCCTCAAGGT GCAGGCAAAT CACACCTAAC TGGTCTATTG CCAGACTATC TGGAAAAACA TTATGGTCTG CGTCTCGCTA CTATGTCTTT GGACGACTTT TACCTCACCC ATTCCGATCA GGTCAAGCTA TCCCAATCCG AGCCCGATAA CCCACTCCTC AACGGCCGCG GCCCCGCCGG TACCCACGAT TTACCACTTC TGGAACAATG TCTGGCAAAG TTGAAATCTA TCAATGACCG CAGTGCGAAG TTTCCAACTG CGACTAAGGA CCAAAGGGCT CAGCTGCCCA TATATGACAA ATCCCTTTTC AAAGGCGAGG GTGACCGGTC AAAGGAGGTA GTAGAAGTGC AAGGACCGAT TGATGTGGTC ATATTTGAAG GGTGGATGAA TGGATTCGGG CCGTTATCAA ATGACAAACT AGAAGAGAAA TACGCCGAAG CCGGACGACA ATGGCCCTCT TCTTCTCTCG TCGGCGCACC TGGGGTCATG CCTACCATTT TATTGTATTC GAGATCAACG CTACACAGTA TAAACCAAAA TCTGAGGCAG TATGAAGTAC TTTGGGATCA AATTGATTGT TTTGTACAGA TTCAACCGTT GGATCTGTCT TACGTATGGA CTTGGAGACT TCAGCAAGAA CACAATATGA AAGCGAAGAA TGGAGGTAAC GGAATGACTG ATGAGCAGGT CCGACATTTC ATTAACCGAT ACATGCCCAG CTATGAGCTG TTTCAAGATG GAATTGACAA GGAGACCACA TCATGGAGGG GCAAAGGGCT TCGTTTTATT GTGAACATAA AAAGAGAAAT TGTTGGCACA GAAAGCTTCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT