SSGCID: Target Page

CrneC.19323.a

Uncharacterized protein

CENTER ID:	CrneC.19323.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19323.a.B3.GE40917	histidine kinase domain 2		1363	1634
CrneC.19323.a.B4.GE40918	PAS domain - histidine kinase domain 2		1196	1638

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_03355
UniProt:	J9VXZ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLGNGSIDYS ALPTRYLESY PYPSLVLLVP IPPPSQPSQE ELDEFPSPSF NTEAPKLFTC ADHGTSQPFD IVWANEKWRN LTNDQPLLNC LNVKGARKLG NWIGGVKEAT GNGKEQIVHV AKAAGVGIPE VLAKMISSQS SSEFSVTQML HSADPGQDRM PQPPEHFWAP EHPYEDRSTK SDHLSNSSEL TGIEVEEGMQ NNPATVIIEL VNPGTVTLEF TKTIMPVTRL GTGGLKAPMN THSFAIITTV PKSLFVPNSS FQSSPLASPE LQGVQDEAAT RSNYPLAIPK TTILSPSQSP PPLPFSTAPQ SSLTPNPDTD NISPAKKSLI DELKGPGILP PSKKHSAPLM FNGDGTVSRQ AQGLEARGES RTSVDVLMDQ TDWSKTPLGP LEQWSQSLKT IVGLVLNYPH QCCLWWGEEL TLIYNEPYAR AIHKHPDIFG MSGPVAWSEI WNSIGPLSEV VLSGTPVWKE DDFLLFKQLP HQGEGVWEEY HTWMWVPVLQ EDGTFGGLWN ATVRTTKKVL AERRTATVRE MGQRTSIART MDEFNDAVCE ILESNPRDTP FAALYRVEPT LSSKGKGLVA NTAEITKNDP NEVQVDVTLV GRVGIPESHP STPSSMSFTI RQRRGSSINR FPQGLNSSPA MSLISSTGGI SHIPLGDIDE GESEPYPTPE LKHWPIREAL QCKRLILVEN VAGLIKDYPI RVWDELPNAA IVVPIRSDSE EGIPSAVLVL GLNIRRPFDE DYESFIHVLR LQLASGIAAV RSYEAERLRL DELAALDRAK SLLFSNVSHE LRTPLTLISG PIEDLLMETK DGPKKEMLLM ARRNLRRLSR LVSTLMDVSR LEAGRLRGTF RPVNLGIMTR DLALLFKATI EKAKLKYIIE VDTSPENVFV DPEHWEKIVF NLIGNAMKYT MTGFVKIELY YSSGQAILTV QDSGVGIPRS DIHLIGERFH RVQSVSRSHE GTGIGLSLTK ELISLHGGTL EIESFTEEES IDGSHGSTFR VKIPIGSDHL PFEAIDTGRM PNMPQLTYGQ GLVDEAMQWV RDRETGSVVS SDESSSALTG DSSGMGNAKP IDPNTLYFQK EDVIMLVEDS ADTRRYMKSI FSQYCQVVEA RDGQEALELC QKSVPDLIIS DVMMPHLNGF ELLVALKRSK DLKMVPVIML TARGADESKV DGIMAGAEDY LAKPFSAREI VARAHMQLQL GKKRRHLEEA FEERTAELRA LAEFSPVGIF RADQNGNITF ANSAWYKVSG YPMNEPIVDW VDVIIAPHER EAARAFWKEA LTSPEKSLMG DWEYANGRWI TVTIIRLDAI SNGSVGLKGL LGCVTDITER KVHEESQRRQ VIEAEQRRLD AEEAKRQQEL LIDITSHEIR NPISSVMQCS SLVKTNLLSL QEQLHIAMAN HTAFIPTRQL MNNIEEDLEA LESIYQCGLT QERISNDVLS LGRIQLDMLQ MFDVEFDLSK EAQNIISIFQ NEARMKRISL SLKTGDNNKK LGIRYVKADL VRLNQITTNL LSNAIRFTAT SSIRHIEVRT DISFDPPEEG TCAVAKDVLS QPPVLKDDMP IFVFLEVADT GPGLSEDECQ KLFQRFSQVS PKTHTIFGGS GLGLFVCRKL AELMGGSIEV VSEKGKGSTF RFYIKAKTCR TSPATDSDLR AKMKAQRDTA RYFGLRRRPH VLIVEDNLIN QTVLARQLKH CNLTCDVAND GLEALEKIRK VSSIENESDG QAFDCVLMDL EMPVMDGLTA VGHIREEENA GKLKKNLVIA LTGNARQGQI DLAKASGMDE VVIKPYRLDD LLHKIEEVIK IRVAEEDEEI NQEVEVETKA VEQARKIATP MESATDKS

NT Sequence

atgttaggaa acggcagcat tgactactcg gcgctgccta cgcggtatct tgagtcctat ccctacccat ccttggtact tttggtccct atacctccgc cttcacaacc ttctcaggaa gagctggacg aatttccatc gccctcgttc aatacagaag cacccaaact attcacgtgt gcggaccacg gaacatcgca gccatttgac attgtttggg caaatgaaaa gtggaggaac ctgaccaatg accagccatt gttgaattgc ctcaatgtga aaggagcgcg taaacttggg aattggattg gaggtgtcaa agaagctacg ggaaacggga aggaacagat cgttcatgtt gcaaaagctg ctggtgtggg gataccggaa gtgctggcaa aaatgatttc aagccaatca tcatcggagt tctctgtaac ccaaatgctt cactcggccg atcctgggca agatcgaatg cctcaaccgc cagaacattt ctgggcgcct gaacatccct atgaagatcg ctcaactaaa tctgaccatt tgtccaattc gagtgagttg actggaattg aagtggagga agggatgcag aacaaccctg cgacagtaat aattgagcta gtgaaccccg ggaccgtgac cttggaattt acaaagacaa tcatgcccgt cacgagatta ggtacaggcg gtttaaaggc gccgatgaac acgcattctt ttgccattat cacaactgta cccaagtccc tctttgtacc caactcatcg ttccagtcaa gcccattggc tagccccgag ctgcagggag tacaggatga agctgccacg aggtccaatt atccccttgc tattcccaag accactatac tgtcaccctc tcaatcgcca cctcctttgc cgttcagtac tgctcctcaa tcatccctta cgccgaaccc tgatacagac aacatttcac ctgcaaaaaa atctctaatc gatgagctca agggccctgg catcttgccg cctagtaaga aacattccgc gccgttgatg ttcaatgggg atggtacagt tagccgccag gcacaagggc tagaagctag aggggaatcg aggaccagtg tggatgtgtt aatggatcag actgattggt cgaagacgcc attgggtccg ttggaacagt ggtcgcaaag tttaaagacc attgtgggtt tggtactcaa ttaccctcat caatgctgcc tttggtgggg cgaagagctt accctgattt acaatgagcc ctatgcccga gcgattcaca aacatccaga catttttgga atgtcaggcc ctgttgcctg gtcagagatc tggaattcaa ttggtccatt atcagaagta gttcttagcg gtaccccagt atggaaggaa gatgatttct tactcttcaa acaacttcct caccaaggag agggtgtttg ggaagagtac catacctgga tgtgggtgcc tgttttgcag gaagatggaa cctttggagg tttgtggaat gcaacggtcc gtaccacaaa gaaagttttg gcggagagga ggacggctac ggtacgggaa atggggcaga gaacatcgat tgcaaggact atggacgaat tcaatgatgc ggtatgcgag atcctggaga gtaaccctcg agatactcca tttgctgctt tgtatcgtgt cgagcctact ttgtcatcaa aaggaaaggg cctggtggca aatactgctg aaatcacgaa gaatgatcca aatgaagtcc aggtggatgt gactctggtc ggaagagttg ggattcctga aagccaccca tcgactccgt cttccatgtc cttcactatt cgacagagac gcggcagctc tatcaaccgt ttccctcagg gtctcaatag ctctccagca atgtccctga tttcatccac cggcggcatt tctcatatcc ctctgggaga tattgacgag ggggagagtg agccttatcc tactcctgag ctgaagcatt ggcctattcg agaggcattg cagtgcaaaa ggctaattct ggtggaaaac gttgctggcc tcatcaagga ttacccgata agggtctggg atgaattacc gaacgcggcg attgtcgtac ccatcagaag tgattcagag gaaggcatac cgagtgcagt attggtctta ggcctaaaca tcagaagacc ttttgatgag gactatgagt cattcattca tgtattaagg ttgcaattgg cgtcaggtat agcggcagta cgttcctacg aggcggaaag actaaggcta gatgaattag ctgcgttgga tcgtgccaaa agtcttctgt tttccaacgt cagtcatgaa ctaaggacac ctctgaccct tatctccggt ccgattgagg atcttttaat ggagaccaaa gatggaccta agaaagagat gctgttaatg gcacgtcgta atctccgccg cctatcacgt ctcgtgtcta ccttaatgga cgttagtcga ctagaagctg gtcgattaag aggaacattc aggcctgtca atctgggtat catgacaaga gatttggcct tgcttttcaa ggcaacgatt gagaaggcca agctcaagta catcatcgaa gtagatactt cacctgaaaa cgtatttgtc gaccctgagc attgggaaaa aatcgttttt aacctgattg gtaacgccat gaagtatacg atgactggtt tcgtcaaaat tgagctatat tactcatctg gccaggcgat tcttactgtg caagatagcg gtgtcggtat tcctcgctca gatatccatc tcatcggcga gcgcttccat cgggtgcaat cggtcagcag gtctcatgag ggaaccggca tcggactgtc gttgacgaaa gaactcatca gtcttcacgg tggtaccctt gagattgaaa gtttcacaga ggaagagtca atagatggct cacatggctc gacattcaga gtcaagatac caattggtag tgaccacttg ccctttgagg ccattgacac cggtcgaatg cctaacatgc cccagttaac atatggacag ggtttagtag acgaagctat gcagtgggtc cgagatcgtg aaactggcag cgtcgtttct tcagacgaat ccagctcggc cctcactggt gattcatcag ggatgggtaa tgccaagccg attgatccca atacactcta cttccagaaa gaggacgtga taatgcttgt agaagactcg gccgatacaa ggcgttacat gaagtcgatt ttcagtcagt actgccaagt cgttgaagct cgcgatggcc aagaagcttt ggagctctgt cagaagtcgg tacccgatct gataatttcc gatgttatga tgcctcacct taatggtttc gaactccttg tggccttgaa aagatccaag gatctcaaaa tggtgcccgt aataatgctg accgcccgtg gagccgatga gtccaaagtt gacggtatca tggctggagc cgaagactat ctcgcgaaac catttagtgc tcgagagatc gtcgctcgtg cccatatgca attgcaactt ggcaaaaagc gtagacatct tgaagaagca tttgaggaac gaacagcaga gcttagagct ctggcagagt tctctcctgt cggtatcttc cgagcagacc agaatggtaa catcacgttt gccaacagcg cttggtacaa ggtgtcaggt tacccaatga atgaaccgat cgtcgactgg gttgatgtca ttattgcccc acatgagcgt gaagcagcta gggcgttctg gaaagaagct ttgacctcgc cggaaaagtc tttgatgggt gattgggagt atgccaatgg ccgttggatc actgtaacaa ttattcgttt agacgcaatt tctaatgggt cggtcggtct caagggtctg cttggatgcg tcacggacat cacggaaaga aaggttcatg aggagtcgca acgccgtcag gtgatagaag ccgagcaacg tagactggat gctgaggaag cgaagcgaca acaggaatta ctcattgaca tcacatccca tgaaattcga aaccctatca gcagtgtaat gcagtgttcc tctctagtca aaaccaacct tctgtccttg caggaacagc tccacatcgc tatggctaac cacacagctt tcattcctac tcgtcagttg atgaacaata tcgaagagga tcttgaagca cttgagagca tctatcaatg tggtcttact caagaaagga tcagtaatga tgtactttcc ttgggaagaa ttcagctaga tatgcttcag atgtttgacg tcgaatttga tctctcaaaa gaggctcaaa acatcatttc aatcttccag aatgaagcga gaatgaaaag aatatcacta tctctcaaga ctggagataa caataagaag ctggggattc gctatgtcaa ggccgatttg gtaaggctca accaaatcac aacaaactta ctgtccaacg ctatccgttt cactgccaca agctctattc gacatatcga agttcgaacc gatattagct ttgatccacc tgaagaagga acttgtgcgg tagctaaaga cgtcctttct cagccgcctg tacttaaaga tgatatgccg attttcgtct ttctcgaagt cgccgacact ggtcccggtt tatccgaaga cgaatgtcaa aaacttttcc aaagattctc acaagtctct ccgaaaactc acaccatctt tggtggatcg ggccttggat tatttgtctg tcgaaaattg gctgaattaa tgggcggcag cattgaagtt gtcagcgaaa agggcaaagg cagcacattc cgcttctaca ttaaagccaa gacatgtcga acgtctcctg caacggatag tgatcttcga gccaaaatga aggctcaacg ggacacagca agatactttg gcttaagaag gagacctcat gttttgattg tagaagataa cctgatcaac cagactgtgc tggcgaggca actgaagcat tgtaatctga cttgcgacgt ggcaaacgat ggcctggaag ccctggaaaa gatcaggaaa gtgtcaagta tagaaaatga atctgacggc caggcgtttg actgtgtgct catggatctt gagatgcctg taatggacgg tctcactgct gttgggcata tcagagaaga agaaaatgct ggaaaattga agaagaacct ggtcatcgcc ctgactggta atgctcgaca agggcaaatc gacctggcga aggcgagcgg gatggatgag gtcgtcatca aaccttatcg attagatgat ttacttcaca agattgagga agtgataaaa attagagtgg cagaagaaga cgaggaaatt aatcaggaag tagaagtaga aaccaaagcg gtggagcaag ctcggaaaat tgcaacgcca atggaaagtg cgactgacaa aagctga

Details for CrneC.19323.a.B3.GE40917

HARVESTED ON:	12/20/2016
SEQUENCED ON:	12/27/2016
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHDI TSHEIRNPIS SVMQCSSLVK TNLLSLQEQL HIAMANHTAF IPTRQLMNNI EEDLEALESI YQCGLTQERI SNDVLSLGRI QLDMLQMFDV EFDLSKEAQN IISIFQNEAR MKRISLSLKT GDNNKKLGIR YVKADLVRLN QITTNLLSNA IRFTATSSIR HIEVRTDISF DPPEEGTCAV AKDVLSQPPV LKDDMPIFVF LEVADTGPGL SEDECQKLFQ RFSQVSPKTH TIFGGSGLGL FVCRKLAELM GGSIEVVSEK GKGSTFRFYI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacatca catcccatga aattcgaaac cctatcagca gtgtaatgca gtgttcctct ctagtcaaaa ccaaccttct gtccttgcag gaacagctcc acatcgctat ggctaaccac acagctttca ttcctactcg tcagttgatg aacaatatcg aagaggatct tgaagcactt gagagcatct atcaatgtgg tcttactcaa gaaaggatca gtaatgatgt actttccttg ggaagaattc agctagatat gcttcagatg tttgacgtcg aatttgatct ctcaaaagag gctcaaaaca tcatttcaat cttccagaat gaagcgagaa tgaaaagaat atcactatct ctcaagactg gagataacaa taagaagctg gggattcgct atgtcaaggc cgatttggta aggctcaacc aaatcacaac aaacttactg tccaacgcta tccgtttcac tgccacaagc tctattcgac atatcgaagt tcgaaccgat attagctttg atccacctga agaaggaact tgtgcggtag ctaaagacgt cctttctcag ccgcctgtac ttaaagatga tatgccgatt ttcgtctttc tcgaagtcgc cgacactggt cccggtttat ccgaagacga atgtcaaaaa cttttccaaa gattctcaca agtctctccg aaaactcaca ccatctttgg tggatcgggc cttggattat ttgtctgtcg aaaattggct gaattaatgg gcggcagcat tgaagttgtc agcgaaaagg gcaaaggcag cacattccgc ttctacattt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for CrneC.19323.a.B4.GE40918

HARVESTED ON:	12/20/2016
SEQUENCED ON:	12/27/2016
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMQ LQLGKKRRHL EEAFEERTAE LRALAEFSPV GIFRADQNGN ITFANSAWYK VSGYPMNEPI VDWVDVIIAP HEREAARAFW KEALTSPEKS LMGDWEYANG RWITVTIIRL DAISNGSVGL KGLLGCVTDI TERKVHEESQ RRQVIEAEQR RLDAEEAKRQ QELLIDITSH EIRNPISSVM QCSSLVKTNL LSLQEQLHIA MANHTAFIPT RQLMNNIEED LEALESIYQC GLTQERISND VLPLGRIQLD MLQMFDVEFD LSKEAQNIIS IFQNEARMKR ISLSLKTGDN NKKLGIRYVK ADLVRLNQIT TNLLSNAIRF TATSSIRHIE VRTDISFDPP EEGTCAVAKD VLSQPPVLKD DMPIFVFLEV ADTGPGLSED ECQKLFQRFS QVSPKTHTIF GGSGLGLFVC RKLAELMGGS IEVVSEKGKG ST

Validated NT Sequence

aatgtgctgc ctttgccctt ttcgctgaca acttcaatgc tgccgcccat taattcagcc aattttcgac agacaaataa tccaaggccc gatccaccaa agatggtgtg agttttcgga gagacttgtg agaatctttg gaaaagtttt tgacattcgt cttcggataa accgggacca gtgtcggcga cttcgaggaa gacgaaaatc ggcatatcat ctttaagtac aggcggctga gaaaggacgt ctttagctac cgcacaagtt ccttcttcag gtggatcaaa gctaatatcg gttcgaactt cgatatgtcg aatagagctt gtggcagtga aacggatagc gttggacagt aagtttgttg tgatttggtt gagccttacc aaatcggcct tgacatagcg aatccccagc ttcttattgt tatctccagt cttgagagat agtgatattc ttttcattct cgcttcattc tggaagattg aaatgatgtt ttgagcctct tttgagagat caaattcgac gtcaaacatc tgaagcatat ctagctgaat tcttcccaag ggaagtacat cattactgat cctttcttga gtaagaccac attgatagat gctctcaagt gcttcaagat cctcttcgat attgttcatc aactgacgag taggaatgaa agctgtgtgg ttagccatag cgatgtggag ctgttcctgc aaggacagaa ggttggtttt gactagagag gaacactgca ttacactgct gatagggttt cgaatttcat gggatgtgat gtcaatgagt aattcctgtt gtcgcttcgc ttcctcagca tccagtctac gttgctcggc ttctatcacc tgacggcgtt gcgactcctc atgaaccttt ctttccgtga tgtccgtgac gcatccaagc agacccttga gaccgaccga cccattagaa attgcgtcta aacgaataat tgttacagtg atccaacggc cattggcata ctcccaatca cccatcaaag acttttccgg cgaggtcaaa gcttctttcc agaacgccct agctgcttca cgctcatgtg gggcaataat gacatcaacc cagtcgacga tcggttcatt cattgggtaa cctgacacct tgtaccaagc gctgttggca aacgtgatgt taccattctg gtctgctcgg aagataccga caggagagaa ctctgccaga gctctaagct ctgctgttcg ttcctcaaat gcttcttcaa gatgtctacg ctttttgcca agttgcaatt gcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHMQ LQLGKKRRHL EEAFEERTAE LRALAEFSPV GIFRADQNGN ITFANSAWYK VSGYPMNEPI VDWVDVIIAP HEREAARAFW KEALTSPEKS LMGDWEYANG RWITVTIIRL DAISNGSVGL KGLLGCVTDI TERKVHEESQ RRQVIEAEQR RLDAEEAKRQ QELLIDITSH EIRNPISSVM QCSSLVKTNL LSLQEQLHIA MANHTAFIPT RQLMNNIEED LEALESIYQC GLTQERISND VLSLGRIQLD MLQMFDVEFD LSKEAQNIIS IFQNEARMKR ISLSLKTGDN NKKLGIRYVK ADLVRLNQIT TNLLSNAIRF TATSSIRHIE VRTDISFDPP EEGTCAVAKD VLSQPPVLKD DMPIFVFLEV ADTGPGLSED ECQKLFQRFS QVSPKTHTIF GGSGLGLFVC RKLAELMGGS IEVVSEKGKG STFRFYIKAK T

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaat tgcaacttgg caaaaagcgt agacatcttg aagaagcatt tgaggaacga acagcagagc ttagagctct ggcagagttc tctcctgtcg gtatcttccg agcagaccag aatggtaaca tcacgtttgc caacagcgct tggtacaagg tgtcaggtta cccaatgaat gaaccgatcg tcgactgggt tgatgtcatt attgccccac atgagcgtga agcagctagg gcgttctgga aagaagcttt gacctcgccg gaaaagtctt tgatgggtga ttgggagtat gccaatggcc gttggatcac tgtaacaatt attcgtttag acgcaatttc taatgggtcg gtcggtctca agggtctgct tggatgcgtc acggacatca cggaaagaaa ggttcatgag gagtcgcaac gccgtcaggt gatagaagcc gagcaacgta gactggatgc tgaggaagcg aagcgacaac aggaattact cattgacatc acatcccatg aaattcgaaa ccctatcagc agtgtaatgc agtgttcctc tctagtcaaa accaaccttc tgtccttgca ggaacagctc cacatcgcta tggctaacca cacagctttc attcctactc gtcagttgat gaacaatatc gaagaggatc ttgaagcact tgagagcatc tatcaatgtg gtcttactca agaaaggatc agtaatgatg tactttcctt gggaagaatt cagctagata tgcttcagat gtttgacgtc gaatttgatc tctcaaaaga ggctcaaaac atcatttcaa tcttccagaa tgaagcgaga atgaaaagaa tatcactatc tctcaagact ggagataaca ataagaagct ggggattcgc tatgtcaagg ccgatttggt aaggctcaac caaatcacaa caaacttact gtccaacgct atccgtttca ctgccacaag ctctattcga catatcgaag ttcgaaccga tattagcttt gatccacctg aagaaggaac ttgtgcggta gctaaagacg tcctttctca gccgcctgta cttaaagatg atatgccgat tttcgtcttt ctcgaagtcg ccgacactgg tcccggttta tccgaagacg aatgtcaaaa acttttccaa agattctcac aagtctctcc gaaaactcac accatctttg gtggatcggg ccttggatta tttgtctgtc gaaaattggc tgaattaatg ggcggcagca ttgaagttgt cagcgaaaag ggcaaaggca gcacattccg cttctacatt aaagccaaga catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat