CrneC.19321.a

D-erythro-sphingosine kinase

CENTER ID: CrneC.19321.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19321.a.B2.GE40913 NAD-kinase like domain 113 311
CrneC.19321.a.B3.GE40914 diacylglycerol kinase-like domain 434 556
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_01165
UniProt: J9VTE7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRPPHHLPVI LHNNNRGLLI IQRSTLDVLQ LSGDGQPPKR LLTCPLTNFL KAYLAPAPEN SARRLDLHCL GGGKGTQLKL VKLHVLVEPI NIPETNKWIR MLMEVAYASI KPFRNVLILV NPLGGKGKAK NMVQDTVIPI LEAAGTTVTV KETTHRLHAE EIARSMDLVY DVIATASGDG LVYEVVNGLA SRSDARKALQ TPIAPIPTGS ANAVCTNLFG VKDTFNVPLA ALNIIKGCNL PIDLCSVLIL PSMTRRFAFL SQAIGLMVDL DIGTENLRWM GDTRFLIGFL KGIASNKGTR CRLRLKVVED DKQDMAKKAK ERTQQGTTEG GVTPLTNRMR NMSVDGDTPV SMNHNIRHEH EPPALIGESK SSANNPPKPA NSNYIPDNGP IPNARPLKYD ETWITIESLP KKSSIKPKKS TQSLKAPRNV STQNEWVDGQ EILYFYAGMM RWVARDLMQW PVSIPGDGLI DVVVQSVVPR LTMANAIAGA EKGETYWMDC QYYYKVSQFI AENLDIENQP LFTIDGEAFP FESFHVEVHT RAANLLSLNG DFFTSDFLKK PTGKS
NT Sequence
atgcgtcctc cacaccacct tcccgtcatc cttcacaata acaatcgtgg cttgctcatc atccaacggt ccactctcga cgttctccag ctctcgggcg acggccagcc tcccaagcga ctactcacat gccctttgac caactttctc aaggcatatc ttgcccctgc tcccgagaat tcagcgcggc ggctggacct gcattgtcta ggagggggca agggcacgca gctgaaactc gtcaaacttc atgtattagt ggaacccatc aacataccgg aaacaaataa gtggatacgg atgctaatgg aggtagctta tgcctcaatc aagcccttca gaaatgtttt gatcctggtc aaccccctag gaggaaaagg taaagcaaag aatatggtac aagatactgt tattcctata cttgaggcag cgggaacaac tgtaaccgtc aaagaaacta ctcacaggct acatgccgaa gagatcgcga gatctatgga tctggtatac gatgtgattg ccactgcttc cggagacggt cttgtttatg aagtggtcaa cggccttgca tcccgctcag acgcccgcaa ggcgttgcag acgcctatcg ctcccatccc aacaggttca gctaatgccg tctgcacaaa cctattcggc gtgaaggaca ctttcaacgt acccttggct gcactcaaca ttatcaaagg atgtaacctc cccattgatc tttgctccgt ccttatactc ccatctatga cccgacgctt cgctttcctt tctcaggcaa ttggcctcat ggtagatctc gacattggta cagaaaatct caggtggatg ggggataccc gctttttaat tgggtttctc aaaggaatag cgagcaataa gggtacgcgt tgccggttga ggctcaaagt cgtcgaagac gacaagcagg acatggctaa aaaggcaaag gaacggacac agcaagggac gactgagggt ggcgttactc cgttgaccaa ccggatgaga aatatgagtg tggacggtga cacgccagtc tctatgaacc ataatattcg ccacgagcac gaacccccag cattgatcgg agaatctaaa tcatccgcca acaatccacc aaagcccgcc aactctaatt atattccgga caatggtccc attccaaatg ctcggccgct aaaatacgat gagacttgga taactattga aagtctcccc aagaagtcat ctatcaagcc caaaaagtct acccagagtt tgaaagcccc ccgtaatgtt tcaacacaaa acgaatgggt tgacggacaa gagattctat atttttatgc tggaatgatg cgttgggtgg ctcgagatct gatgcaatgg cctgtctcca ttccaggaga tggcctgata gatgtagttg tccaaagcgt agtaccgagg ctgactatgg caaatgcaat tgccggcgcc gaaaaaggag agacatattg gatggattgc caatattact ataaggtttc acagttcatc gcagagaact tagacataga aaatcaacca cttttcacca tcgatggcga agccttcccc tttgaatcgt ttcatgtcga ggtacatact cgagctgcca atcttcttag cctaaacgga gactttttca cttctgattt cttgaagaag cccactggaa agagttaa
Details for CrneC.19321.a.B2.GE40913
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 22kDa
OBSERVED MW: 22kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMF RNVLILVNPL GGKGKAKNMV QDTVIPILEA AGTTVTVKET THRLHAEEIA RSMDLVYDVI ATASGDGLVY EVVNGLASRS DARKALQTPI APIPTGSANA VCTNLFGVKD TFNVPLAALN IIKGCNLPID LCSVLILPSM TRRFAFLSQA IGLMVDLDIG TENLRWMGDT RFLIGFLKGI ASNKGTRCRL RLKVVEDD
Validated NT Sequence
atcttactca cttagtcgtc ttcgacgact ttgagcctca accggcaacg cgtaccctta ttgctcgcta ttcctttgag aaacccaatt aaaaagcggg tatcccccat ccacctgaga ttttctgtac caatgtcgag atctaccatg aggccaattg cctgagaaag gaaagcgaag cgtcgggtca tagatgggag tataaggacg gagcaaagat caatggggag gttacatcct ttgataatgt tgagtgcagc caagggtacg ttgaaagtgt ccttcacgcc gaataggttt gtgcagacgg cattagctga acctgttggg atgggagcga taggcgtctg caacgccttg cgggcgtctg agcgggatgc aaggccgttg accacttcat aaacaagacc gtctccggaa gcagtggcaa tcacatcgta taccagatcc atagatctcg cgatctcttc ggcatgtagc ctgtgagtag tttctttgac ggttacagtt gttcccgctg cctcaagtat aggaataaca gtatcttgta ccatattctt tgctttacct tttcctccta gggggttgac caggatcaaa acatttctga acatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHFR NVLILVNPLG GKGKAKNMVQ DTVIPILEAA GTTVTVKETT HRLHAEEIAR SMDLVYDVIA TASGDGLVYE VVNGLASRSD ARKALQTPIA PIPTGSANAV CTNLFGVKDT FNVPLAALNI IKGCNLPIDL CSVLILPSMT RRFAFLSQAI GLMVDLDIGT ENLRWMGDTR FLIGFLKGIA SNKGTRCRLR LKVVEDD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttcagaa atgttttgat cctggtcaac cccctaggag gaaaaggtaa agcaaagaat atggtacaag atactgttat tcctatactt gaggcagcgg gaacaactgt aaccgtcaaa gaaactactc acaggctaca tgccgaagag atcgcgagat ctatggatct ggtatacgat gtgattgcca ctgcttccgg agacggtctt gtttatgaag tggtcaacgg ccttgcatcc cgctcagacg cccgcaaggc gttgcagacg cctatcgctc ccatcccaac aggttcagct aatgccgtct gcacaaacct attcggcgtg aaggacactt tcaacgtacc cttggctgca ctcaacatta tcaaaggatg taacctcccc attgatcttt gctccgtcct tatactccca tctatgaccc gacgcttcgc tttcctttct caggcaattg gcctcatggt agatctcgac attggtacag aaaatctcag gtggatgggg gatacccgct ttttaattgg gtttctcaaa ggaatagcga gcaataaggg tacgcgttgc cggttgaggc tcaaagtcgt cgaagacgac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for CrneC.19321.a.B3.GE40914
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 15kDa
OBSERVED MW: 14kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN EWVDGQEILY FYAGMMRWVA RDLMQWPVSI PGDGLIDVVV QSVVPRLTMA NAIAGAEKGE TYWMDCQYYY KVSQFIAENL DIENQPLFTI DGEAFPFESF HVEVHTRAAN LLSLNGDFFT SD
Validated NT Sequence
atcttactca cttaatcaga agtgaaaaag tctccgttta ggctaagaag attggcagct cgagtatgta cctcgacatg aaacgattca aaggggaagg cttcgccatc gatggtgaaa agtggttgat tttctatgtc taagttctct gcgatgaact gtgaaacctt atagtaatat tggcaatcca tccaatatgt ctctcctttt tcggcgccgg caattgcatt tgccatagtc agcctcggta ctacgctttg gacaactaca tctatcaggc catctcctgg aatggagaca ggccattgca tcagatctcg agccacccaa cgcatcattc cagcataaaa atatagaatc tcttgtccgt caacccattc gttcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHNE WVDGQEILYF YAGMMRWVAR DLMQWPVSIP GDGLIDVVVQ SVVPRLTMAN AIAGAEKGET YWMDCQYYYK VSQFIAENLD IENQPLFTID GEAFPFESFH VEVHTRAANL LSLNGDFFTS D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataacgaat gggttgacgg acaagagatt ctatattttt atgctggaat gatgcgttgg gtggctcgag atctgatgca atggcctgtc tccattccag gagatggcct gatagatgta gttgtccaaa gcgtagtacc gaggctgact atggcaaatg caattgccgg cgccgaaaaa ggagagacat attggatgga ttgccaatat tactataagg tttcacagtt catcgcagag aacttagaca tagaaaatca accacttttc accatcgatg gcgaagcctt cccctttgaa tcgtttcatg tcgaggtaca tactcgagct gccaatcttc ttagcctaaa cggagacttt ttcacttctg attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat