SSGCID: Target Page

CrneC.19320.a

Uncharacterized protein

CENTER ID:	CrneC.19320.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19320.a.B2.GE40911	histidine kinase domain		807	1113
CrneC.19320.a.B3.GE40912	PAS domain-histidine kinase		726	1113

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_06278
UniProt:	J9VVM9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDHNGSKPP SQDASSTVPV RNKKTSSTAE NVEHVVSGPP TTQEEWDRFI TDYTMGDLTQ RSFLPTKSAR STSALGESIV GQDVVSQASV LPVSPTLSGQ MPSTSLPIAS SETSRAQVLE TIASKSLDDT DILDENSQEP YAIHLNTSSS GPSNQDPDIP NIPMTNEPGQ SCSTSKPAEG KTKKFKHPPD MTRENVEKLG DEYKMKAQRG KKASSKQEKK VKVRPMKGDY REPSEEESKK LRKRRARRPR SRGSWVSRRE QSFDDPLLPA ARDAPLDPAL LDSATNSSTS ISLADLGHNF AAERIRVREY FQKNGYLPPP RQTEEASRRR LRVIRRLGLE NAEKFQVETI DRLARLAVSF FKTNAAVISV IGKSKQLFLS EIGFGARTCE VDMSVCYHAM TMSQAGDQCI VINDASKDWR FRKNPLVDEG RGPVQFYAGS PLRVGQGSKE AIIGTLCVVD DKPRDDFGED QRELLADLAK CVVSELELLY SQQAAVESAK LHQISVDFLR RSLKHRPLEH AGRSAGTATG SGSTPMGSGR AGNDGSSGSG STRSLGSQKR IGGADEQSDE MVDIYDEACR EIRFALDAYA VAVVDLSQFH VFFPAYQNSS TGGGSTRAGS VMGTMTGSGA TTVRPGASSS MDQSTSIAST VGEEDNPWDS KKKQARPTYS MNDPMAPVRT PQVLFIPSRP RAKSKWYNKS TEQQSGDVDT LAVLGYSCAY DNYAFNFTTS PAARKIVADF IASNVTTRKM WYTRDDSEGI AASITHLMPP GTETSLALPV FGFDGQVSFA VVACWKDPLY TYPAGAMQFV ETIAGSLLAS VLKERLLQAE RAQLNFASAA SHELRTPLHQ INAAANILRT SLQPVLQSSD APGELPSRLS ADDRQEVLAQ LDLIDSNSLS LAGILENVID TLDVGKMSES QGNENRQMPD IVTPRDTSRA VSLSAALEEV VDDAMELESK TRRALGGKGL EDVEVILEVL PRQRGGWLTT KDIGPLARAT GKVIHNAIKF TDKGHVHITV QDISREVILP TGYDNSLKVS TISIDIKDSG RGMSADFLDR EILQPFSKED PFISGSGLGL TLTQRILELI GGKIAIASSP GKGTLVHIEV PVQFLNDDSN SDQDDLGRND GAPDAIEESN AIRTDGIYLI GFATAKNSAL RRVGKSLTRQ LKLHKCRVVT EINYASLIVA PEGEVRDAEL ARLCRSARPC VQVIVLERDR TKYGSIYTPS HLYAHTNPLQ SRSSPAATLL TPQDQEYLDR IPIIHLTRPI RPSVVRRIMD PAPGVSRKSE QYVSDVVGGD EAKEEAAIGA RPSIDHVQKP PSASAPFSEG AEFKPRPITS ANPAVTTSTA PPATLEGGSS SDVGSGSEMQ NEDQPSVSSS SISGSDTRSG TEGLSGTSVS ANGTNGASES AVYGGSETSE TSETSNRSTT EVNGKNEGKS DKTSKQSMKG LNLLKVLVVE DNVINRKILT TMLRRVSCSF AEAVDGVDAV DQFSVFQPDL VLLDITMPRK DGFAAAAEMR RLETVRQIAE KDVPNPSSPI AAELIENALG SLDLNAHAPG SSSVSSSPGS SHSIKLKKRA RIIAVTAMSA EHQKRKGLYE CGIDHWMTKP LSMSVLRSMV EKMKEEINEG T

NT Sequence

atgtccgatc acaatgggag caagcctcct tcacaagatg cctcttcgac tgtcccggtt cggaataaga agacctcatc tacagctgag aatgtcgagc atgttgtgag cggtccacct acgacacagg aagaatggga taggttcata acggactaca ccatgggcga ccttactcaa cgatcctttc ttccaaccaa gtcagcacgc tcgacatctg ccctgggtga atccattgtt ggccaggatg tcgtatccca agcatccgtt ctccctgttt ctcccactct gtccggtcag atgccttcaa cttcccttcc catcgcttca tcggagacat ccagagctca agtattggaa acaattgcca gtaagagcct tgatgacact gatatcctgg atgagaacag tcaggagccc tatgctattc acctcaatac gtcttcttct ggtccttcga accaagatcc tgatatcccc aacattccca tgaccaacga gcccggacaa tcttgttcta caagcaaacc cgcggaggga aagacgaaaa agttcaaaca tccgccagat atgactcggg agaacgtgga gaagcttggt gacgagtaca aaatgaaggc acagcgcgga aagaaagcgt catcaaaaca ggaaaagaag gtgaaagttc ggccaatgaa gggagattac agggaaccat ctgaagaaga atcaaaaaag ttgcgtaaaa gacgtgcacg tcggccgagg tcgcgagggt cttgggtcag tcgacgtgag cagtcatttg atgatccact ccttcctgct gctcgagatg cgccattaga tccagcatta ttagactctg ctaccaattc ttctacatct atttcgctag ccgaccttgg tcacaacttc gcggcagaaa gaattagagt gagagagtat tttcagaaga acggatatct tcccccaccg cgacaaacag aagaagcatc ccgacggcgt ctacgagtca ttcgacgact tggccttgaa aatgccgaaa agttccaggt tgaaaccatt gacagactcg cccgtcttgc cgtttcattt ttcaagacca acgcggcagt catcagtgtc atcggcaaaa gcaaacagct gttcctttcc gaaatcggct ttggcgcacg aacatgcgag gtggacatgt cagtatgcta tcacgcaatg acaatgtcac aagccggaga tcagtgtatt gtaattaacg atgcgagcaa agattggcgg tttaggaaga atccgcttgt cgatgaaggg agaggaccag tgcagttcta cgctggctcg cccttgaggg taggacaagg gtcgaaagag gctattatcg ggactctgtg tgtagtggac gacaagccga gagacgattt tggagaggat cagcgtgagc tgttggcgga tcttgcaaag tgtgtagtga gcgagttgga attactttac tctcaacaag ctgctgtcga aagcgccaag cttcaccaga tctccgtgga ctttttacga cgttcgctta aacatcgacc attagagcat gcggggagat cagcaggtac ggcaactggt tccggctcga cgcctatggg aagtggcaga gctggtaacg atggttcatc tgggagtggg tcgacaagaa gcttagggag tcaaaaacgg attggaggag cagacgagca gagcgatgag atggtcgata tctacgacga ggcatgtcga gagatccgat ttgctttgga cgcgtacgct gttgctgtcg tcgatctgtc gcaattccat gtcttttttc ccgcttacca aaactcctca acgggcggtg gatcaactcg agcaggatca gtgatgggga cgatgacggg gagcggagcg actactgtca gacctggggc gtcttcaagc atggaccaaa gcacttcgat cgcttcgact gttggagagg aggacaaccc ttgggactcg aagaagaaac aggctcgtcc gacctactcc atgaacgatc ctatggcacc ggttcgtacc cctcaagtcc tttttattcc ttctcgaccg cgagcaaagt caaaatggta taataaaagt acagaacagc agtcaggcga cgttgataca ttagcagtgc tgggctacag ttgtgcctat gacaactatg cttttaactt tactacttca ccggcggcga gaaagattgt tgccgacttc attgcgagca atgtcactac aaggaagatg tggtacacac gggatgatag tgagggtatc gccgctagca taacccacct catgcctccg ggtactgaga cgagtttggc attgcctgta tttggcttcg acggtcaggt ctcatttgcg gttgtcgcgt gctggaaaga tcctctctac acatatcctg ctggtgcaat gcagttcgtt gaaacaattg ctggtagttt actggcgtct gtgttaaagg aacggttact ccaagcagag cgagcacagt tgaactttgc ttcagccgcc agtcatgaac tcagaacacc ccttcaccaa atcaatgctg ccgcgaatat ccttcgaact tcactacaac ctgttttaca gtcttctgat gcacccggcg aattgccttc acggctatcg gcggatgatc gacaagaggt gctagcacaa ttagatctga tagatagcaa cagtttatcg ctggcaggta ttctggagaa cgttatcgac acgttggatg tgggcaagat gtccgagtct caaggcaatg agaaccgcca aatgcccgat atcgtcacac cacgagatac ttcgcgagca gtcagtttgt ccgccgcttt ggaggaggtg gtcgacgatg ctatggagtt ggagtcaaag acgaggcgcg ccttgggagg aaaaggtttg gaggatgtgg aggtcatact ggaagtgttg ccgaggcaga ggggtggatg gttaacgacc aaagatattg ggccattggc gagagctact gggaaggtca ttcacaacgc gatcaaattt acagacaagg gccatgtgca catcactgtc caagacatat ctcgcgaggt catcttgcct actggctatg ataattctct caaagtatcc acaatatcaa tcgatatcaa agacagtgga cgtggaatgt ctgccgattt ccttgatcga gagatccttc aacctttttc caaggaggat ccctttattt ctggttctgg acttggcctc acactcactc aacgcatcct cgaactcatc ggaggcaaga tcgccattgc ctcgtcgccc ggcaagggta cattggtcca tattgaagtc ccagttcaat tccttaacga tgatagcaac tccgaccaag atgatcttgg tcgtaatgat ggtgcgcctg acgcaataga agagtcgaat gccattcgaa cggatggtat ctatctcatc ggatttgcga cggccaagaa ctctgcatta cgtcgtgtcg gcaagtctct cacacggcag ttgaaacttc acaagtgccg agtggtaacc gagatcaatt acgccagctt gatcgtggcg ccggagggag aagtaaggga tgctgagttg gcgagattgt gtcgatcagc gagaccttgt gtgcaagtga ttgttttgga aagggaccgg actaaatacg gttccattta cactccaagt cacttgtatg cccataccaa tcctctacaa tcgcgttcca gtccggcagc aactctcctc acacctcaag accaggagta tctggaccgt attcctatca tccaccttac aaggcccata cgacctagcg ttgtaagacg tatcatggat cctgctcctg gcgtgtccag aaaatctgag caatacgtat cagacgtcgt tggaggtgat gaggcaaagg aagaagcggc gataggcgcg aggccatcta ttgaccatgt tcaaaaacca ccatcagcca gcgctccgtt tagtgaaggt gccgaattca aaccacgtcc gataacatca gcaaaccctg cagttacaac atctacagct ccacctgcga cattggaggg aggtagctcg agtgatgtgg gaagtgggag cgagatgcag aatgaagatc agccatctgt gtcctccagt tcgataagcg gtagcgatac gcgtagtggg acggaaggac tgtcggggac gtcagtctcg gctaatggaa caaatggagc tagtgagagt gcagtgtacg gtggaagtga gactagcgag actagcgaaa ctagcaaccg ttcaacaacg gaagtaaatg gtaaaaatga ggggaagtcg gacaaaacca gtaagcaatc gatgaaaggc cttaatttac ttaaagtgct tgtcgtggag gataatgtga tcaatcggaa gatcctgacg accatgcttc gacgagtgtc ttgcagcttc gctgaagccg ttgatggggt cgacgctgtg gatcaatttt cagtttttca acctgatttg gttcttctcg acataacgat gccacgcaaa gacggtttcg ctgccgctgc ggaaatgcgt cggctcgaaa cagtccgtca gatcgccgaa aaagatgttc ccaatccttc aagccctatc gccgccgaat taatcgagaa tgcattagga tcacttgacc tcaacgccca tgcaccagga tcttcaagtg tcagttcatc tcctggaagc agccacagca tcaaattgaa gaagagagca aggatcattg cagtaacggc catgagtgct gaacaccaaa agaggaaggg gctatacgaa tgtggtattg atcattggat gaccaagccg ttgagtatga gtgtgttgag atcgatggta gagaaaatga aggaagaaat caatgagggc acataa

Details for CrneC.19320.a.B2.GE40911

HARVESTED ON:	12/20/2016
SEQUENCED ON:	12/27/2016
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMQ FVETIAGSLL ASVLKERLLQ AERAQLNFAS AASHELRTPL HQINAAANIL RTSLQPVLQS SDAPGELPSR LSADDRQEVL AQLDLIDSNS LSLAGILENV IDTLDVGKMS ESQGNENRQM PDIVTPRDTS RAVSLSAALE EVVDDAMELE SKTRRALGGK GLEDVEVILE VLPRQRGGWL TTKDIGPLAR ATGKVIHNAI KFTDKGHVHI TVQDISREVI LPTGYDNSLK VSTISIDIKD SGRGMSADFL DREILQPFSK EDPFISGSGL GLTLTQRILE LIGGKIAIAS SPGKGTLXXI

Validated NT Sequence

ttcaatatnn nccaatgtac ccttgccggg cgacgaggca atggcgatct tgcctccgat gagttcgagg atgcgttgag tgagtgtgag gccaagtcca gaaccagaaa taaagggatc ctccttggaa aaaggttgaa ggatctctcg atcaaggaaa tcggcagaca ttccacgtcc actgtctttg atatcgattg atattgtgga tactttgaga gaattatcat agccagtagg caagatgacc tcgcgagata tgtcttggac agtgatgtgc acatggccct tgtctgtaaa tttgatcgcg ttgtgaatga ccttcccagt agctctcgcc aatggcccaa tatctttggt cgttaaccat ccacccctct gcctcggcaa cacttccagt atgacctcca catcctccaa accttttcct cccaaggcgc gcctcgtctt tgactccaac tccatagcat cgtcgaccac ctcctccaaa gcggcggaca aactgactgc tcgcgaagta tctcgtggtg tgacgatatc gggcatttgg cggttctcat tgccttgaga ctcggacatc ttgcccacat ccaacgtgtc gataacgttc tccagaatac ctgccagcga taaactgttg ctatctatca gatctaattg tgctagcacc tcttgtcgat catccgccga tagccgtgaa ggcaattcgc cgggtgcatc agaagactgt aaaacaggtt gtagtgaagt tcgaaggata ttcgcggcag cattgatttg gtgaaggggt gttctgagtt catgactggc ggctgaagca aagttcaact gtgctcgctc tgcttggagt aaccgttcct ttaacacaga cgccagtaaa ctaccagcaa ttgtttcaac gaactgcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagt tcgttgaaac aattgctggt agtttactgg cgtctgtgtt aaaggaacgg ttactccaag cagagcgagc acagttgaac tttgcttcag ccgccagtca tgaactcaga acaccccttc accaaatcaa tgctgccgcg aatatccttc gaacttcact acaacctgtt ttacagtctt ctgatgcacc cggcgaattg ccttcacggc tatcggcgga tgatcgacaa gaggtgctag cacaattaga tctgatagat agcaacagtt tatcgctggc aggtattctg gagaacgtta tcgacacgtt ggatgtgggc aagatgtccg agtctcaagg caatgagaac cgccaaatgc ccgatatcgt cacaccacga gatacttcgc gagcagtcag tttgtccgcc gctttggagg aggtggtcga cgatgctatg gagttggagt caaagacgag gcgcgccttg ggaggaaaag gtttggagga tgtggaggtc atactggaag tgttgccgag gcagaggggt ggatggttaa cgaccaaaga tattgggcca ttggcgagag ctactgggaa ggtcattcac aacgcgatca aatttacaga caagggccat gtgcacatca ctgtccaaga catatctcgc gaggtcatct tgcctactgg ctatgataat tctctcaaag tatccacaat atcaatcgat atcaaagaca gtggacgtgg aatgtctgcc gatttccttg atcgagagat ccttcaacct ttttccaagg aggatccctt tatttctggt tctggacttg gcctcacact cactcaacgc atcctcgaac tcatcggagg caagatcgcc attgcctcgt cgcccggcaa gggtacattg gtccatattg aagtcccagt tcaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for CrneC.19320.a.B3.GE40912

HARVESTED ON:	12/20/2016
SEQUENCED ON:	12/27/2016
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMN FTTSPAARKI VADFIASNVT TRKMWYTRDD SEGIAASITH LMPPGTETSL ALPVFGFDGQ VSFAVVACWK DPLYTYPAGA MQFVETIAGS LLASVLKERL LQAERAQLNF ASAASHELRT PLHQINAAAN ILRTSLQPVL QSSDAPGELP SRLSADDRQE VLAQLDLIDS NSLSLAGILE NVIDTLDVGK MSESQGNENR QMPDIVTPRD TSRAVSLSAA LEEVVDDAME LESKTRRALG GKGLEDVEVI LEVLPRQRGG WLTTKDIGPL ARATGKVIHN AIKFTDKGHV HITVQDISRE VILPTGYDNS LKVSTISIDI KDSGRGMSAD FLDREILQPF SKEDPFISGS GLGLTLTQRI LELIGGKIAI ASSPGKGT

Validated NT Sequence

aatgtaccct tgccgggcga cgaggcaatg gcgatcttgc ctccgatgag ttcgaggatg cgttgagtga gtgtgaggcc aagtccagaa ccagaaataa agggatcctc cttggaaaaa ggttgaagga tctctcgatc aaggaaatcg gcagacattc cacgtccact gtctttgata tcgattgata ttgtggatac tttgagagaa ttatcatagc cagtaggcaa gatgacctcg cgagatatgt cttggacagt gatgtgcaca tggcccttgt ctgtaaattt gatcgcgttg tgaatgacct tcccagtagc tctcgccaat ggcccaatat ctttggtcgt taaccatcca cccctctgcc tcggcaacac ttccagtatg acctccacat cctccaaacc ttttcctccc aaggcgcgcc tcgtctttga ctccaactcc atagcatcgt cgaccacctc ctccaaagcg gcggacaaac tgactgctcg cgaagtatct cgtggtgtga cgatatcggg catttggcgg ttctcattgc cttgagactc ggacatcttg cccacatcca acgtgtcgat aacgttctcc agaatacctg ccagcgataa actgttgcta tctatcagat ctaattgtgc tagcacctct tgtcgatcat ccgccgatag ccgtgaaggc aattcgccgg gtgcgtcaga agactgtaaa acaggttgta gtgaagttcg aaggatattc gcggcagcat tgatttggtg aaggggtgtt ctgagttcat gactggcggc tgaagcaaag ttcaactgtg ctcgctctgc ttggagtaac cgttccttta acacagacgc cagtaaacta ccagcaattg tttcaacgaa ctgcattgca ccagcaggat atgtgtagag aggatctttc cagcacgcga caaccgcaaa tgagacctga ccgtcgaagc caaatacagg caatgccaaa ctcgtctcag tacccggagg catgaggtgg gttatgctag cggcgatacc ctcactatca tcccgtgtgt accacatctt ccttgtagtg acattgctcg caatgaagtc ggcaacaatc tttctcgccg ccggtgaagt agtaaagttc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHNF TTSPAARKIV ADFIASNVTT RKMWYTRDDS EGIAASITHL MPPGTETSLA LPVFGFDGQV SFAVVACWKD PLYTYPAGAM QFVETIAGSL LASVLKERLL QAERAQLNFA SAASHELRTP LHQINAAANI LRTSLQPVLQ SSDAPGELPS RLSADDRQEV LAQLDLIDSN SLSLAGILEN VIDTLDVGKM SESQGNENRQ MPDIVTPRDT SRAVSLSAAL EEVVDDAMEL ESKTRRALGG KGLEDVEVIL EVLPRQRGGW LTTKDIGPLA RATGKVIHNA IKFTDKGHVH ITVQDISREV ILPTGYDNSL KVSTISIDIK DSGRGMSADF LDREILQPFS KEDPFISGSG LGLTLTQRIL ELIGGKIAIA SSPGKGTLVH IEVPVQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataacttta ctacttcacc ggcggcgaga aagattgttg ccgacttcat tgcgagcaat gtcactacaa ggaagatgtg gtacacacgg gatgatagtg agggtatcgc cgctagcata acccacctca tgcctccggg tactgagacg agtttggcat tgcctgtatt tggcttcgac ggtcaggtct catttgcggt tgtcgcgtgc tggaaagatc ctctctacac atatcctgct ggtgcaatgc agttcgttga aacaattgct ggtagtttac tggcgtctgt gttaaaggaa cggttactcc aagcagagcg agcacagttg aactttgctt cagccgccag tcatgaactc agaacacccc ttcaccaaat caatgctgcc gcgaatatcc ttcgaacttc actacaacct gttttacagt cttctgatgc acccggcgaa ttgccttcac ggctatcggc ggatgatcga caagaggtgc tagcacaatt agatctgata gatagcaaca gtttatcgct ggcaggtatt ctggagaacg ttatcgacac gttggatgtg ggcaagatgt ccgagtctca aggcaatgag aaccgccaaa tgcccgatat cgtcacacca cgagatactt cgcgagcagt cagtttgtcc gccgctttgg aggaggtggt cgacgatgct atggagttgg agtcaaagac gaggcgcgcc ttgggaggaa aaggtttgga ggatgtggag gtcatactgg aagtgttgcc gaggcagagg ggtggatggt taacgaccaa agatattggg ccattggcga gagctactgg gaaggtcatt cacaacgcga tcaaatttac agacaagggc catgtgcaca tcactgtcca agacatatct cgcgaggtca tcttgcctac tggctatgat aattctctca aagtatccac aatatcaatc gatatcaaag acagtggacg tggaatgtct gccgatttcc ttgatcgaga gatccttcaa cctttttcca aggaggatcc ctttatttct ggttctggac ttggcctcac actcactcaa cgcatcctcg aactcatcgg aggcaagatc gccattgcct cgtcgcccgg caagggtaca ttggtccata ttgaagtccc agttcaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at