CrneC.19318.a

Atypical/PIKK/FRAP protein kinase

CENTER ID: CrneC.19318.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19318.a.B2.GE40909 mTor kinase domain 1249 2260
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_06642
UniProt: J9VNA0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSQSDVLDN IFQRLSARSE DVRAQAGQDL AEHVVAYTQE YPGHDASKGV WAQVFHKTFE FTRSNNQLER LGAIIAISQL LQLTKDDTPD RAQQKVLRLY EYLRPLTTCG DSTVMLPASL VVEDMVRNSP TLHTDTFLGK EVGQALVMID DSRQEVGRFS GALLLYAFAR AAPGVFHQYI SKVLEKIWIP LRDSRSVVRE RASMLLSTCL DTLKTRGDRP STDTYRKIFE EARLGLLKAS STESILGSLL AFNSMLQNQQ LSMAEYYRSI CELTFKYRDS KEVSIRKAVI ALIPSMATYD SDDFEAHYLH RGMAYLLQAL NRPADRDISY VALGHMAVHL GSKMKPFIDD IMRIIRDHLR MRGKKNAPYE APIFQCLAML ATSVGPMLTR QMHEILDLMF PWGLSEPLCT ALQATASHIP PLLRTIQDRL LEMLSQTLTG QSYRPLGAPA PRGGAQMDLN LLQSTTNAQS TDTLKLALRL LARFDFVGHT LSEFVRDAAL PYLEHDSVEV RREAVLATTT LFMTDPICQQ TSSNSVEIVN DVLSKLLTVA ITDPNAGIRR TVLDHLEDKF DRHLAQAADI RCLFIALNDE VFGNRERTIS IIGRLAHHNP AYVMPHLRKS LINIVTELEY STNARQKEES AKLLCLMIGA AAGLVKSYAP TILSVLLRTA SSPESSIGVQ AECLKCIGEL ARVAGEELVP SVRAILDLVI EMLNDQSSPA KRDTALKTLG QIASNTGEVI KPYTDYPQLM GVLFRFLRME ANSSVRQETI KTIGMLGALD PFKHKTLLGD VDDPIDEGTT SRVNDIVLLN QHNSSVNDEF FQTVVIHSLV NVLHDSTYKD HYQAVEAIMM IFRTQRLRCV NFLPQIVPAF LNVIRIAHSS RTELYLKQLA QFITIVKLHI RNYLNDVFDL IHEFWNPNST LQITIISLVE AIAKAVEGEF KAYLPKLLQQ ILRSFDGDLS AKHLPELKLN TLLQILKAFY VFGESIEDYL HLVLPVIVRS FENPAAPDSL RIAALRTTGQ LCRKVNFSDH ASQIIHPLVR TLGNSSEELR QTAMETLCVL VLQFGPDYAI FIPMVNKALV ENKISHPGYE ALITKLLNRE RLPPDLGPVE RYASDSAAEA SAPEPVALKV NQQALKLAWD CSHLLNTSSR TEWISWIIGL GHEMMRESPS QAIRAARSLA LSSVAFTKEL FNVAFYSCWQ ELFESYQEDL WHNLDRAIKK DDVPGDVVNM ILGATQFLEH DEKEVAIESR VLGSVAANYQ ALAVALHYKE QEFFLDPSKE VIEDLIDVNQ KLQQSDAAWG TLEWAQTEMG MTTEVEWYEK LGRWEEALQV WNERDADAST TFSEWEITEG KVTCLHAMGE WEQLSDFVQA RWANRTAEEK KLLSPLAAAA SWSLKQWDLM DDYISAMKGD GADRAFFKAI LAVHRNQIPA ALKQISKARE RLDPELTTLT GDSYGRAYDT VVRIQMLAEL EEIIAYKDHA DEPARQEMQR QTWKKRLAGC QRDVEVWQRI LQVRSLVLKP NEDMDTWIEF ADLCRTSDRL NLAEKTLTSL VGFQYPSMED TRGRAPPPII FAYLRMAWAK NLQIDSREER YETLQHLRDF TDQLTDDVGI GARGPNGRLM LPDQKLYGSY TKLLAQCHVE LGQWQATLRE SQGSADPSGI LHDYCLATEL DPEWYQAWHT WALANFEVIT QLEVSQQGLS PVHFTTYIIP AVEGFLKSIS LSPGNSLQDT LRLLTLWFTY GYSSGVTAAV SQGLPTVNID VWLEVIPQII ARIQTPRQSI QQLIVQLLHD IGKAHPQALI YPLTVASKST VAARRTVAQN ITHKMREHSP KIVDQAELVS TELIRAAILW HEMWYDGLEE ASKHYFGDHD IPGMLGVLEP LHEIVENGPQ TLRETSFIQS FGHDLRIARE HLKRYRITQD GTEIQQAWDV YYSVFQRLGK QLKLLNVIEL QYVSPKLMAV RDLDIAVPGT YQSGKPIIGI KNVIPTFKVI ASKQKPRQCS MRGMDGKEYA YCLKGHEDLR QDERVMQLFG LVNTLLNNDH ESAKRHLSIQ RFSVTPLSPS AGLLGWVTHS DTIHVLIKQY RDQRKILVDI EHKLMQQMSD ESYDSLPLLH KVEIFQYALD NTTGQDLYRI LWLKSRNSDI WLERRTTYTR SLGLNSMVGY ILGLGDRHPS NLLLDQITGK MVHIDFGDCF EVAQQRDKYP EKVPFRLTRM LIHAMEVCGI TGNFSRSCEV SMEVLRDNRE SLMAVLEAFV YDPLIAWRLT ATDKRPGGVG EVKDLDDPAV YGKQRKNKAN ETEILNDVEN TEVKNDKGLQ VIERVRRKLT GRDFKPDVVL DVKSQVEKLV VEATKTENLC VAFLGWCSFW
NT Sequence
atgtcttcac aatcagacgt tctcgacaac atctttcagc gtctgtccgc caggtcggaa gatgtccgcg cccaggccgg ccaagatctt gcagagcacg tcgtagctta cacccaagaa tacccgggac atgatgcttc gaagggtgta tgggcacagg tctttcacaa gacattcgag tttactagga gcaacaatca gctagagaga ttaggtgcta ttattgccat atcgcaattg ttacaattaa ccaaggatga cacaccggat cgtgctcagc agaaggttct acgcctctat gaatatcttc gacctctcac aacatgcggc gattcgaccg tcatgcttcc cgcctctctt gtcgtggaag atatggtccg caattccccc acgcttcaca ccgatacttt ccttggcaaa gaagttggac aagcgctggt aatgattgat gactcacgtc aagaagtcgg ccgtttcagt ggtgccctcc ttctatatgc tttcgctcgt gctgccccag gagtattcca tcagtacatc tccaaggtct tggaaaagat ttggatcccc cttcgtgact ctagatctgt cgttcgagag cgcgcgagca tgctcttatc tacctgcctt gacactctca aaacccgagg agaccgacca tccaccgata cctaccgcaa gatctttgaa gaggcccgtc ttggtctcct caaagccagc tcgacagagt ctatcttggg ttctctcctt gcgttcaatt ccatgctcca aaatcaacag ctttcgatgg cggaatatta ccgctccatt tgcgaactaa ccttcaaata ccgtgattcg aaggaagtct ccatcaggaa ggctgtcatt gcccttatac catctatggc gacatatgac agcgacgact tcgaggcaca ttatttgcat aggggtatgg cgtatctgct acaagctttg aacagaccgg cagacaggga catttcgtat gtggcgttgg gtcatatggc tgtacatctt ggatcgaaaa tgaagccttt tattgacgac attatgcgaa tcattcggga tcatctacgc atgcgaggca aaaaaaatgc cccatacgaa gcccccatct tccagtgtct tgcgatgctt gctacgtctg tcgggcctat gcttacccgc cagatgcacg aaatcctcga cctcatgttc ccctggggcc tttccgaacc cttatgcaca gctcttcagg ccaccgcctc acatatccct cctctattga gaactatcca agataggttg ttggagatgc tttcgcagac cttgaccgga caaagctata gaccgttagg tgcgcctgcc ccaagaggtg gagctcagat ggatcttaac cttttgcaat ctacaaccaa cgcccagtca acagacactc tcaaacttgc tcttcgtctc cttgcgcgct tcgacttcgt cggtcatacc cttagcgagt ttgtccgtga tgctgctctt ccgtacctcg aacacgacag cgtcgaggtc cgacgtgaag ctgtgttggc aaccacaacc ctgtttatga cggacccaat ctgtcagcag acgagcagta actcggtcga gattgtaaat gatgtgttga gtaaattgtt aactgtagcc attaccgatc ctaatgctgg aatccgccgg acggtacttg atcaccttga agacaagttt gatcgccatc tcgctcaggc tgccgatatc cgatgtctct ttatcgctct caacgatgaa gtgtttggta atcgtgaaag gaccatctcg atcattggga gattggcaca ccataacccg gcttatgtga tgcctcattt gagaaagagt ttgatcaata ttgtcactga gttggagtat tctaccaatg cgcgacaaaa ggaagagagt gccaaactgc tctgtcttat gatcggcgct gccgctggtc tcgttaagtc ttatgcccct accatcttgt ccgttctcct ccgcactgct tccagccccg aatcttctat cggtgttcaa gccgaatgcc tcaaatgtat tggcgagctc gcaagagttg cgggcgaaga actcgtccct tccgtccgag ccattctcga cctcgtcatt gaaatgctca acgaccagtc ttcccccgcc aagagagata ctgccctcaa gaccttgggg cagattgcga gtaacacagg agaggttatc aagccctata cggattaccc tcagttaatg ggggtgctgt ttaggttttt gagaatggag gcgaattcga gtgtcaggca agagactatc aagaccattg gtatgctggg tgccttggat cccttcaagc acaagacttt gttgggtgat gtggacgacc ctatcgacga agggacaacc tcgcgagtca atgacattgt gttgctcaac caacacaact cttcagtcaa cgacgagttc ttccagactg ttgtcatcca ttctttagtt aacgtcttgc atgactctac ttacaaggat cattatcaag ccgtggaagc cattatgatg atttttagga ctcaacgatt gagatgtgtc aacttccttc ctcagattgt ccctgcattc ctcaatgtca ttcgcattgc acattcctct cgtaccgagc tttacctcaa acaacttgcg cagtttatta caatcgttaa gctgcatatc cgcaactacc tcaatgatgt ctttgatctc atccacgagt tttggaaccc caactctacc cttcagatca ccatcatctc cctcgttgag gccattgcga aggccgtgga aggagaattc aaggcatact tgcccaagct tttgcagcaa attttgagat cgtttgatgg agacttgtca gccaagcacc ttccggagct gaaactcaac actttgcttc agatcctgaa agcgttctac gtctttggcg aatctattga agactatctt caccttgtgt tgcccgtcat tgttcgatct tttgagaacc cggccgcccc cgactctctc cgcattgctg ctttgcgcac tacaggacaa ctttgtcgta aagtcaactt ttcggaccac gcaagtcaga tcatccaccc gttagtaaga actttgggta acagctcaga ggagttgagg cagaccgcaa tggaaacact ttgtgttttg gtgttgcagt tcggtccaga ttatgctatc ttcattccta tggtaaacaa agccttggtg gaaaacaaaa tatcacaccc tggttacgaa gccctcataa ccaagttgct caaccgcgag cgccttcctc ctgaccttgg tcctgttgaa agatacgcca gcgactctgc cgccgaagcc tctgccccag agcctgttgc tcttaaggtc aaccagcagg ctcttaaact ggcttgggac tgttcccatt tgcttaacac cagcagcagg accgagtgga tctcttggat tattggcttg ggtcatgaga tgatgagaga aagccctagt caagccataa gagctgcgag gagtttggcg ttgagtagtg tggccttcac caaagaactg tttaatgttg ctttctactc atgctggcag gagctgtttg agagctacca ggaggattta tggcacaatc ttgaccgagc aatcaaaaaa gatgatgtcc ccggcgatgt tgtcaacatg attctcgggg ctacccagtt cttggaacac gatgaaaagg aggtagcgat cgaaagccgt gttttgggca gcgtcgctgc caattatcaa gctctcgcag tcgctttaca ctacaaagaa caggaattct tcctagatcc cagcaaagaa gtcattgaag acctcattga cgtcaaccag aaactccagc aaagcgatgc tgcctggggt acccttgaat gggcccagac agagatggga atgaccactg aagtcgagtg gtacgaaaag cttggaaggt gggaggaagc gttacaagtg tggaacgagc gagatgccga tgcgtcgacc actttctcag agtgggagat cactgaaggc aaggtcactt gtctgcacgc tatgggcgaa tgggaacaac tctctgattt tgtccaggct cgatgggcca acaggacggc tgaggagaag aagttgcttt caccattggc ggcagcggct agttggtcgc tgaaacagtg ggatctgatg gatgactaca tctctgccat gaagggcgat ggagcagatc gcgcgttctt caaggctatc ctagctgtgc acagaaacca aattcctgcc gctttgaagc agatctccaa ggcacgagaa aggttggatc cagagttgac taccttaact ggtgacagct atggccgagc gtacgacact gtcgtgagga ttcaaatgct tgctgagctg gaggaaatca ttgcctacaa ggaccacgcc gacgagcctg cccgacagga gatgcaacga cagacttgga agaagcggct ggccggatgt cagcgtgatg ttgaagtttg gcagcgcatt cttcaggtca gatcccttgt gctcaagcct aatgaggata tggacacttg gattgagttt gcggatctct gtcgaacatc tgacaggctg aacttggccg agaaaacgct gacatctctt gttggcttcc aatacccatc aatggaagac actcgaggac gagcgccacc tcccatcatc ttcgcttacc tccgtatggc ttgggcgaag aacctacaaa tcgactctcg agaagaacgt tacgagactc tccaacacct gcgcgacttc accgaccagc ttaccgacga cgttggtatt ggagcgaggg gacccaacgg cagactcatg ttacctgacc agaagttgta tggatcttac accaagctgc tggcgcagtg ccacgttgag ttgggtcagt ggcaagcgac cttgagggaa agccaaggat ctgctgatcc ctcgggcatt ctccacgact actgtctcgc cactgagctt gaccctgagt ggtaccaagc ttggcacact tgggccttgg ccaatttcga ggtcattacc cagcttgaag tgtcgcagca aggtctctca cctgttcact ttacgaccta tatcattcct gctgtcgaag gtttcctcaa gtctatctcg ctttctcccg gcaactcctt gcaagatact ttgaggctgt tgactctctg gtttacatat ggatattcta gcggagtcac tgcggctgtt agccaaggtc ttcccactgt caacattgat gtctggctcg aggttattcc tcagatcatt gcccgtatcc agacgcctcg ccagtctata cagcagctca ttgtacaatt gttgcatgat attggcaaag cccatcccca agcccttatt taccctctta ccgtcgcctc caaatctaca gtcgctgccc gacgcactgt tgcccaaaat attacgcata aaatgcgaga gcactctccc aagattgttg accaggccga gcttgtcagt acagagctca tccgagcagc tatcttatgg catgagatgt ggtatgatgg tttggaagaa gcgtcaaagc actactttgg tgaccacgat atccctggca tgttaggagt ccttgagcct ttgcatgaga ttgtcgaaaa cggacctcaa accttgcgtg agacatcctt tatccaatcg ttcggccatg atttgcgtat cgctcgggag catctcaagc gttaccgtat aactcaggat ggtactgaaa tccaacaagc atgggatgtc tattattccg tcttccagcg tctgggcaaa cagctcaagc tcctcaacgt cattgagctg caatatgtct cgcccaagtt aatggccgtt cgagacttgg atattgcagt tccaggtacc taccagagtg gcaagcctat catcggtatt aagaacgtta tcccaacctt caaggtcatt gcttccaagc aaaagccaag acagtgcagc atgcgcggta tggatggcaa ggaatatgca tattgcctca agggtcacga ggacttgcga caagacgagc gtgtcatgca actctttggt ttggtcaaca ctcttcttaa taatgaccac gagtctgcca aacgacatct cagtatccag cgattttctg ttactcctct ttcccctagt gccggtttac tcggttgggt tacccacagt gatacaattc atgttctcat caaacaatac cgagaccaaa ggaaaatctt ggtggatatt gagcacaaac ttatgcagca gatgtctgat gagagctacg attctctacc gctcttgcac aaggtcgaga tcttccagta tgctttggat aacacgactg gtcaagattt gtaccgcatc ctgtggctca agtctcgtaa ctcagatatc tggcttgaaa gaaggaccac ctacaccaga agtctgggtc tcaattccat ggtcgggtat atccttggtc tgggtgacag acatccttca aacctgttgc tcgatcagat tacgggtaaa atggtgcaca ttgactttgg tgattgtttc gaggttgctc aacagagaga caaataccct gagaaagtac ctttccgact tacccgaatg ctcattcatg ccatggaggt ctgcggtatc actggtaact tttcgcgaag ttgtgaagtg tccatggaag tccttcgtga caacagggaa tcacttatgg ccgtccttga agcatttgtg tacgaccctc ttattgcttg gcgtcttaca gcgacggaca aacgacctgg tggtgttggc gaagtcaagg acctggatga cccggctgtg tacggaaagc agaggaaaaa caaggcgaac gagacggaga ttctcaatga tgtggaaaat accgaggtga agaacgacaa gggtctgcag gtcattgaac gagtacgacg aaaattgacc ggtcgagact ttaagcccga tgttgtactc gatgtcaagt cccaggtgga gaagttggtg gttgaggcga caaagacaga gaatctttgt gtagcattct tgggatggtg ttcgttctgg taa
Details for CrneC.19318.a.B2.GE40909
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 117kDa
OBSERVED MW: 117kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHSR VLGSVAANYQ ALAVALHYKE QEFFLDPSKE VIEDLIDVNQ KLQQSDAAWG TLEWAQTEMG MTTEVEWYEK LGRWEEALQV WNERDADAST TFSEWEITEG KVTCLHAMGE WEQLSDFVQA RWANRTAEEK KLLSPLAAAA SWSLKQWDLM DDYISAMKGD GADRAFFKAI LAVHRNQIPA ALKQISKARE RLDPELTTLT GDSYGRAYDT VVRIQMLAEL EEIIAYKDHA DEPARQEMQR QTWKKRLAGC QRDVEVWQRI LQVRSLVLKP NEDMDTWIEF ADLCRTSDRL NLAEKTLTSL VGFQYPSMED TRGRAPPPII FAYLRMAWAK NLQIDSREER YETLQHLRDF TDQLTDDVGI GARGPNGRLM LPDQKLYGSY TKLLAQCHVE LGQWQATLRE SQGSADPSGI LHDYCLATEL DPEWYQAWHT WALANFEVIT QLEVSQQGLS PVHFTTYIIP AVEGFLKSIS LSPGNSLQDT LRLLTLWFTY GYSSGVTAAV SQGLPTVNID VWLEVIPQII ARIQTPRQSI QQLIVQLLHD IGKAHPQALI YPLTVASKST VAARRTVAQN ITHKMREHSP KIVDQAELVS TELIRAAILW HEMWYDGLEE ASKHYFGDHD IPGMLGVLEP LHEIVENGPQ TLRETSFIQS FGHDLRIARE HLKRYRITQD GTEIQQAWDV YYSVFQRLGK QLKLLNVIEL QYVSPKLMAV RDLDIAVPGT YQSGKPIIGI KNVIPTFKVI ASKQKPRQCS MRGMDGKEYA YCLKGHEDLR QDERVMQLFG LVNTLLNNDH ESAKRHLSIQ RFSVTPLSPS AGLLGWVTHS DTIHVLIKQY RDQRKILVDI EHKLMQQMSD ESYDSLPLLH KVEIFQYALD NTTGQDLYRI LWLKSRNSDI WLERRTTYTR SLGLNSMVGY ILGLGDRHPS NLLLDQITGK MVHIDFGDCF EVAQQRDKYP EKVPFRLTRM LIHAMEVCGI TGNFSRSCEV SMEVLRDNRE SLMAVLEAFV YDPLIAWRLT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagccgtg ttttgggcag cgtcgctgcc aattatcaag ctctcgcagt cgctttacac tacaaagaac aggaattctt cctagatccc agcaaagaag tcattgaaga cctcattgac gtcaaccaga aactccagca aagcgatgct gcctggggta cccttgaatg ggcccagaca gagatgggaa tgaccactga agtcgagtgg tacgaaaagc ttggaaggtg ggaggaagcg ttacaagtgt ggaacgagcg agatgccgat gcgtcgacca ctttctcaga gtgggagatc actgaaggca aggtcacttg tctgcacgct atgggcgaat gggaacaact ctctgatttt gtccaggctc gatgggccaa caggacggct gaggagaaga agttgctttc accattggcg gcagcggcta gttggtcgct gaaacagtgg gatctgatgg atgactacat ctctgccatg aagggcgatg gagcagatcg cgcgttcttc aaggctatcc tagctgtgca cagaaaccaa attcctgccg ctttgaagca gatctccaag gcacgagaaa ggttggatcc agagttgact accttaactg gtgacagcta tggccgagcg tacgacactg tcgtgaggat tcaaatgctt gctgagctgg aggaaatcat tgcctacaag gaccacgccg acgagcctgc ccgacaggag atgcaacgac agacttggaa gaagcggctg gccggatgtc agcgtgatgt tgaagtttgg cagcgcattc ttcaggtcag atcccttgtg ctcaagccta atgaggatat ggacacttgg attgagtttg cggatctctg tcgaacatct gacaggctga acttggccga gaaaacgctg acatctcttg ttggcttcca atacccatca atggaagaca ctcgaggacg agcgccacct cccatcatct tcgcttacct ccgtatggct tgggcgaaga acctacaaat cgactctcga gaagaacgtt acgagactct ccaacacctg cgcgacttca ccgaccagct taccgacgac gttggtattg gagcgagggg acccaacggc agactcatgt tacctgacca gaagttgtat ggatcttaca ccaagctgct ggcgcagtgc cacgttgagt tgggtcagtg gcaagcgacc ttgagggaaa gccaaggatc tgctgatccc tcgggcattc tccacgacta ctgtctcgcc actgagcttg accctgagtg gtaccaagct tggcacactt gggccttggc caatttcgag gtcattaccc agcttgaagt gtcgcagcaa ggtctctcac ctgttcactt tacgacctat atcattcctg ctgtcgaagg tttcctcaag tctatctcgc tttctcccgg caactccttg caagatactt tgaggctgtt gactctctgg tttacatatg gatattctag cggagtcact gcggctgtta gccaaggtct tcccactgtc aacattgatg tctggctcga ggttattcct cagatcattg cccgtatcca gacgcctcgc cagtctatac agcagctcat tgtacaattg ttgcatgata ttggcaaagc ccatccccaa gcccttattt accctcttac cgtcgcctcc aaatctacag tcgctgcccg acgcactgtt gcccaaaata ttacgcataa aatgcgagag cactctccca agattgttga ccaggccgag cttgtcagta cagagctcat ccgagcagct atcttatggc atgagatgtg gtatgatggt ttggaagaag cgtcaaagca ctactttggt gaccacgata tccctggcat gttaggagtc cttgagcctt tgcatgagat tgtcgaaaac ggacctcaaa ccttgcgtga gacatccttt atccaatcgt tcggccatga tttgcgtatc gctcgggagc atctcaagcg ttaccgtata actcaggatg gtactgaaat ccaacaagca tgggatgtct attattccgt cttccagcgt ctgggcaaac agctcaagct cctcaacgtc attgagctgc aatatgtctc gcccaagtta atggccgttc gagacttgga tattgcagtt ccaggtacct accagagtgg caagcctatc atcggtatta agaacgttat cccaaccttc aaggtcattg cttccaagca aaagccaaga cagtgcagca tgcgcggtat ggatggcaag gaatatgcat attgcctcaa gggtcacgag gacttgcgac aagacgagcg tgtcatgcaa ctctttggtt tggtcaacac tcttcttaat aatgaccacg agtctgccaa acgacatctc agtatccagc gattttctgt tactcctctt tcccctagtg ccggtttact cggttgggtt acccacagtg atacaattca tgttctcatc aaacaatacc gagaccaaag gaaaatcttg gtggatattg agcacaaact tatgcagcag atgtctgatg agagctacga ttctctaccg ctcttgcaca aggtcgagat cttccagtat gctttggata acacgactgg tcaagatttg taccgcatcc tgtggctcaa gtctcgtaac tcagatatct ggcttgaaag aaggaccacc tacaccagaa gtctgggtct caattccatg gtcgggtata tccttggtct gggtgacaga catccttcaa acctgttgct cgatcagatt acgggtaaaa tggtgcacat tgactttggt gattgtttcg aggttgctca acagagagac aaataccctg agaaagtacc tttccgactt acccgaatgc tcattcatgc catggaggtc tgcggtatca ctggtaactt ttcgcgaagt tgtgaagtgt ccatggaagt ccttcgtgac aacagggaat cacttatggc cgtccttgaa gcatttgtgt acgaccctct tattgcttgg cgtcttacat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat