CrneC.19317.a

Gluconokinase

CENTER ID: CrneC.19317.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19317.a.B2.GE40908 kinase domain 17 230
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_03048
UniProt: J9VGW6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSKPQQELA VPSRLPDPVL IIVMGPASCG KSTVGQDVAD HFHIPFVDGD SLHPKSNIAK MSSGTPLTDE DRLPWLALIR STAERMCKEQ SDEAGGKTRN EEDGGLGRTG VVIACSALKK WYRDILRGVV KADPPPAKDL HEALPDNAQH PATSSLKTYF VYCYGTPELL KQRIAARKGH FFSQTMLDSQ LAVLEDPSGE EDVTVANIDG TKEEVGERAV SGMKKLLGIN
NT Sequence
atgtcttcca agcctcaaca agaattggca gtcccgtcgc gcttgcctga cccggttctt atcatcgtca tgggccctgc atcatgcggt aaatccaccg tcggccaaga cgtcgctgat catttccaca tcccttttgt cgacggcgac tctcttcacc ccaaatcgaa cattgccaaa atgtcctctg gtaccccttt gacagatgag gaccgtctcc cttggcttgc actcatccgt tccacagcgg agaggatgtg taaagagcag tcggacgagg ctggagggaa aaccaggaat gaggaggatg gtgggctggg caggacggga gtggtgattg cctgctcggc tttgaagaag tggtataggg atatattgcg aggtgttgtc aaggctgatc cgcctcctgc taaggatctt catgaggcac ttcccgacaa tgctcaacac ccagcaacat cgtctctcaa aacgtacttt gtctactgct acggtacccc cgaacttctc aagcaacgta tcgccgctcg aaagggccac tttttctcac aaacgatgct cgatagccag cttgcagtat tggaagatcc gtccggggag gaagatgtga ctgtggcgaa catagatggg acaaaggagg aagtcgggga gcgggcggtc agtgggatga agaagctact tgggataaat tag
Details for CrneC.19317.a.B2.GE40908
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 24kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD PVLIIVMGPA SCGKSTVGQD VADHFHIPFV DGDSLHPKSN IAKMSSGTPL TDEDRLPWLA LIRSTAERMC KEQSDEAGGK TRNEEDGGLG RTGVVIACSA LKKWYRDILR GVVKADPPPA KDLHEALPDN AQHPATSSLK TYFVYCYGTP ELLKQRIAAR KGHFFSQTML DSQLAVLEDP SGEEDVTVAN IDGTKEEVGE RAVSGIKKLL GIN
Validated NT Sequence
atcttactca cttaatttat cccaagtagc ttctttatcc cactgaccgc ccgctccccg acttcctcct ttgtcccatc tatgttcgcc acagtcacat cttcctcccc ggacggatct tccaatactg caagctggct atcgagcatc gtttgtgaga aaaagtggcc ctttcgagcg gcgatacgtt gcttgagaag ttcgggggta ccgtagcagt agacaaagta cgttttgaga gacgatgttg ctgggtgttg agcattgtcg ggaagtgcct catgaagatc cttagcagga ggcggatcag ccttgacaac acctcgcaat atatccctat accacttctt caaagccgag caggcaatca ccactcccgt cctgcccagc ccaccatcct cctcattcct ggttttccct ccagcctcgt ccgactgctc tttacacatc ctctccgctg tggaacggat gagtgcaagc caagggagac ggtcctcatc tgtcaaaggg gtaccagagg acattttggc aatgttcgat ttggggtgaa gagagtcgcc gtcgacaaaa gggatgtgga aatgatcagc gacgtcttgg ccgacggtgg atttaccgca tgatgcaggg cccatgacga tgataagaac cgggtccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHDP VLIIVMGPAS CGKSTVGQDV ADHFHIPFVD GDSLHPKSNI AKMSSGTPLT DEDRLPWLAL IRSTAERMCK EQSDEAGGKT RNEEDGGLGR TGVVIACSAL KKWYRDILRG VVKADPPPAK DLHEALPDNA QHPATSSLKT YFVYCYGTPE LLKQRIAARK GHFFSQTMLD SQLAVLEDPS GEEDVTVANI DGTKEEVGER AVSGMKKLLG IN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacccgg ttcttatcat cgtcatgggc cctgcatcat gcggtaaatc caccgtcggc caagacgtcg ctgatcattt ccacatccct tttgtcgacg gcgactctct tcaccccaaa tcgaacattg ccaaaatgtc ctctggtacc cctttgacag atgaggaccg tctcccttgg cttgcactca tccgttccac agcggagagg atgtgtaaag agcagtcgga cgaggctgga gggaaaacca ggaatgagga ggatggtggg ctgggcagga cgggagtggt gattgcctgc tcggctttga agaagtggta tagggatata ttgcgaggtg ttgtcaaggc tgatccgcct cctgctaagg atcttcatga ggcacttccc gacaatgctc aacacccagc aacatcgtct ctcaaaacgt actttgtcta ctgctacggt acccccgaac ttctcaagca acgtatcgcc gctcgaaagg gccacttttt ctcacaaacg atgctcgata gccagcttgc agtattggaa gatccgtccg gggaggaaga tgtgactgtg gcgaacatag atgggacaaa ggaggaagtc ggggagcggg cggtcagtgg gatgaagaag ctacttggga taaattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat