SSGCID: Target Page

CrneC.19314.a

Uncharacterized protein

CENTER ID:	CrneC.19314.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19314.a.B2.GE40907	kinase domain		1487	1799

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_06161
UniProt:	T2BPW5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDSSRHNLHG LPPTYLAHLK ALLHQWLTQQ TTPAGDSKLW SEKRVAQIER AIWEGAVLTS AERGSRRESG ILEQNWEGWI GGSTKRKSLW KEETRQEEEK IKEDEKTEAH VSRKSSMGDR DRGRLRLMVA QAGSTASTPS NASPRASSVA STERRQGTDS APRDVNKGKT PEQSDQEKLQ EWCRIVSTLD GYEPLSLAKA DGWEDINTDR VPKSTHQHIP GSFPQSGLSI EYRHSLDNPS QSKGTFSTLG LPSSPPKHDP SNQSYDLLKP VFCLHFPISH SSRTSTKGLA RKEGSQSTIR IRRDPSVSAR SASSAGSSRW WGSGNKWGDI LGSPFLQDDE EERVEVDFVQ GRFALPSDLF GSPALERRES KKGRVTKRKR RRSIFSALEV VSSSLSTNSS LPSESTSDTT SEAESGQSDT DTDDEMSGKE IKRRIPSRRH RPLSLILSGA EDDIPRSAAE EGKIRLVGGT IVVKGLRGGE RKALGEVLKA LIWTIGMMKL ELDLYTAFRL PHEPDPSTTF ALDVSSSVPL IHPALSPQTS NPTPRPSGEV SRSSSLREKD RSRGQGSREG KKGFLSKLGK NTKGVWNGLL GRKGSARDKS HGQSDMGGEE YTSPILSPTL PSESSPSSPL FPSQPLSSHL PSPSLSSPSS FPRFKYSSYP KDPSPDSNSS FHTNPNINTD ANSNANTNAN PPSQHLQTLS TLSTLLPSST PGLKYPMPPI LLRIQEEERI RREKTQKETL AMSENSNGSG DTSGNTLRGR AMAYRPGGDV RSGLHVLTAG IDTLAGWTRL QLLCVLYCVG LPSPVSGSKS GSIANGGEQL GEDVKGEADE EKGGEGEICE SPQPQFLPPT LTIGGYLQHL GEELGENLLC TRSGCEKEVG QHVRWYVHAG VKVGLRIENV KVKEDGGRDD DGVREDEDEG AVAGKAEDGQ DVEGWVKCIV CREESEPRQL NEGALAYPWS KLLELLIYTP HLQPPICDHH TTPHAYITLF RTSSYTASLT SSPVKLYDVR LPKLQIGPNI PKRKAGGGRQ GMGLMMDDEK EGENALKREV TRWCEALDDR INILREKIER EYEAAKELSE GPEKSGPGLQ EKFDTLLSLS TIFTAFKDNS LESISSTPQD LFNDLRISFS AFIRSSLSEI EAWEKKYLPE NAEEGLETMK EMIPEYAKEG GRVHALPGSG VLVKEDEPAS VVAYTLSSLA YLIELTNTSK PADDSNPATA ITTPRDPTDS SLPIAPTTGA GTPTSASTPS GSGWSTKIEH RDSPRDLLSL RSIVKKKSDA SVSFDKAKFT PSALGLSTLS GGAPSLELNL EKVEGVSEGV EGRDRMEEIA KAVGKATGQE MTLGTTTAHA VPSSLSRRAS MTESDTSSSS GVRLSSPATK SSQGPPSSFR PIRSASSTPI SRSPMSPLPT ATRSPASGAT TTSTLSPNTA ISPGARDSSW DSVTSSFVNS VNNLWKLGSE MGETFSSMRS RPRDQSLKSL MGPLSSMDNS LSTLSPRPHL HFTYTFADKL RLSCTVYFAT AFDTLRRRCA IDKVLVQSLE RTEVWDAQGG KSKAGFWMTK DKRFIVKELL SKWTVSDMHA LLEISPAYFH HMAGTHNRAT ALAKIVGFYT VTIKDSQCGQ KRQLDLLVME NLFHNQNILK TFDLKGIEGR RVAKTKEGDK EVKSTTTQFD ADWLEGMQKG LVLLQPHAKR ILLDAISLDT RFLSSQSIMD YSLLLGVDTR SSLLPSPNIS EDKLDQEREN GLLIVGIVDA IGSFNLFKTI ESRGKMVMNR GGDVTVIPPD QYRERFENAI RHYFVACPDK WSKVSRKVGG FNGCEVSSVL

NT Sequence

atggattcgt cccgccacaa cctccacggc ttaccgccca cctatctcgc tcacctcaag gcactcctcc atcagtggct cactcagcag acaactcccg ccggcgattc taaattgtgg tcagaaaaac gagtagccca gatagagaga gcaatatggg aaggtgcagt actgacttca gctgaaagag ggtcgaggcg ggagagcggt attctcgagc aaaattggga aggttggata ggcgggtcga caaagaggaa gagtttatgg aaggaagaaa ctcggcaaga ggaagagaaa atcaaggaag atgaaaagac cgaggcgcat gtttcgagaa agtcttcaat gggcgacagg gacagaggaa ggcttaggtt gatggttgcg caagctgggt cgacggcatc aacacccagc aacgccagtc cgcgagcttc gtccgttgct tcaactgaac gtcgccaagg cacagatagt gcgcctcgag atgtcaacaa ggggaaaact cccgaacagt ctgaccagga aaaattgcag gaatggtgtc gcatcgtcag cacccttgat gggtatgaac ctttatcact ggccaaagca gacggttggg aagatatcaa taccgatcga gtacccaagt ccacacatca acatatccca ggctcattcc ctcagtcagg cctctctatt gaataccgcc actctctaga caacccatcg caatccaaag gcaccttctc caccctaggc cttccttcct ctccgccaaa gcacgaccct tctaaccaat catacgacct cctcaaaccc gtcttctgcc tgcatttccc catttctcac tcttctcgca ctagtactaa aggactcgca cgcaaggaag gcagccagtc gacgattcgg ataaggcgtg atccgtccgt ttctgcacga tctgcgagta gtgcagggtc gagtcggtgg tggggttcgg gcaataaatg gggagatata cttgggagtc cttttctgca agatgatgaa gaggagaggg tagaggtgga ttttgtgcag ggcaggtttg cgttaccgtc cgacttgttt ggttcccccg cattggaacg acgagagagt aaaaagggga gagtaactaa gagaaaaagg aggcgatcaa tcttttcggc cttggaggtg gtctcatcct cactctcgac aaacagttca ttaccatccg aatctacgtc ggatactact tctgaggccg aatctggaca gtcagatact gatacagacg atgaaatgtc aggaaaagaa ataaaacgtc ggatacctag ccgtcgacat cgaccgctca gcttgatact tagtggcgca gaagacgata tccctcggtc agctgctgaa gaaggtaaaa ttcggctcgt tggtgggacc atcgttgtaa aaggtctccg aggcggggaa aggaaagctc tgggggaagt tttgaaagcg ctcatatgga cgataggaat gatgaagctt gaactcgatc tttacaccgc tttccgcctc cctcacgaac ctgacccttc tactaccttc gccctcgacg tttcatcctc agttccactg attcacccag ccctttcccc tcagacatcc aatcctactc ctcgtccttc cggtgaggtt tctcggagca gctcgttgcg agagaaagat cgttcaaggg ggcagggaag tcgggagggg aagaaagggt ttttatcaaa gttgggtaaa aacacgaaag gcgtatggaa tggattgttg ggacgaaaag ggtctgcgag ggataagtct catggccagt ctgacatggg aggagaagag tatacaagtc ctatactttc gcctacccta ccctcggaat cttccccttc ctcgccgctt ttcccaagtc agcccttgag ctcccatctt ccctctccat ccctctcttc accttcctct tttccgagat tcaaatattc gtcttaccca aaagatccca gcccggactc caactcttct tttcacacca accccaacat caatacagat gccaactcta atgccaatac caatgccaac ccaccttccc aacaccttca aaccctttcg accctctcta ccctcctccc atcctctacg ccaggcctca aataccctat gccgcctatc ctcctgcgaa tacaggagga agaacggatc cggcgggaaa aaacccaaaa agaaactttg gctatgagcg agaatagcaa cggaagcgga gatacgagtg gaaatacatt gagaggaaga gcgatggctt acaggcccgg aggggatgta cgttccggtc tacatgtttt gacagctgga atagataccc tcgcagggtg gacgaggttg cagcttttat gcgtgctata ctgtgtcggt ctaccatcac ctgtttccgg atccaaatca gggtcaattg ccaacggggg agagcagtta ggtgaagatg tgaaagggga agcggatgaa gaaaagggtg gtgaaggaga gatatgtgaa tccccgcagc cacaattttt accacccacg ctgaccatcg ggggttactt gcaacatctg ggagaggagt tgggagagaa tctgctctgt acaaggagtg ggtgtgagaa ggaggtaggg cagcatgtga ggtggtatgt acatgctggg gtgaaggtgg gattgagaat tgagaatgtc aaggtgaagg aagatggggg cagggatgat gatggggtgc gtgaggacga ggacgaaggg gccgttgcag ggaaggctga ggatgggcag gatgtggagg ggtgggtcaa gtgtattgta tgtagagaag agagtgagcc tcgtcagctt aatgaaggcg ccctggcata tccatggtca aaactcctcg aacttttgat atacacgccc catcttcagc cacccatctg cgaccaccac accacccctc acgcatatat cacactcttc cgtacatctt cctatacggc ctcactcacg tcatcgcctg tgaagttgta tgatgtaaga ttgcccaagt tgcagatagg gccgaatatt ccgaagcgta aagctggagg ggggcgtcaa gggatggggt tgatgatgga tgatgagaag gagggagaaa atgcgttgaa gagggaggtg acaaggtggt gtgaggctct ggacgatcgt atcaatattt tgagggagaa aattgaaaga gagtatgaag ccgctaaaga gctctccgaa ggacctgaaa aatccggacc aggtttacaa gagaaatttg ataccctctt gagtctgtcc actatcttta cggctttcaa agacaactcg ctcgaaagca tctcttccac tccacaagat ctcttcaatg acctccgtat atcattttct gcgttcatca gatcaagcct ttcggagatt gaggcatggg agaagaaata tttaccagag aatgcggagg aggggttgga aacgatgaag gaaatgatac cagagtatgc taaagaaggg ggaagggtac atgcgcttcc gggaagcggt gtgctggtaa aggaggatga gccagcgagt gttgtcgcgt atactctttc atccctggcg tacttaatcg aattgactaa cacctcaaaa cccgcggacg actccaaccc tgctacggcc atcaccaccc ctagagatcc aactgactct tctctcccta tagctccaac tacgggcgct ggtactccca cgtccgcctc aactccttct ggcagtggat ggtcaaccaa aattgagcat cgtgactccc cccgagatct tctctctctc cgctccattg tcaagaagaa atccgatgca tcagtgtcat ttgacaaagc aaaattcacc ccttcagcct tgggactttc caccctatcc ggcggcgcgc caagtttaga gctcaacctt gaaaaggtgg aaggggtctc agaaggagtg gaagggaggg ataggatgga ggagattgcc aaggctgttg gtaaggccac gggacaggaa atgactttgg gaactacgac cgcacatgca gtcccttcta gtctgagtag gagagcgagt atgacggaaa gtgataccag tagctcatcc ggtgttcgct tgtcatcccc agcaacaaaa agttcgcaag gcccacccag tagttttcga cccattagaa gtgcttcaag tacccccata tcccgttcac ccatgtcgcc tttaccaact gctacaaggt ctcctgcgtc aggggctacg acaacaagca cactctctcc taatacggcc attagtcctg gggcaagaga ttcttcgtgg gactccgtca catcttcgtt tgtcaattcg gtgaacaatc tctggaaact tggctcagag atgggtgaga ctttttcttc catgcgatct cgccctcgag atcagtctct aaaatcactc atgggacctt tgtcttctat ggataattct ttatctactc tttcaccccg tcctcatctc cattttacct acaccttcgc cgataagctc cggctatcct gtacagttta ttttgcgacg gcgttcgaca cattaaggag aaggtgtgcg atagacaagg tgcttgtcca aagcctcgag aggaccgaag tatgggacgc gcagggtggg aagagtaaag cgggtttttg gatgacgaaa gataagaggt ttattgtgaa ggagctgttg agcaagtgga ctgtcagtga tatgcacgca ttgctggaga tttcacctgc atacttccac cacatggcag ggactcacaa cagggccaca gctttagcca aaatcgtagg attttataca gtgactatca aggactccca atgtggccaa aaacggcaac ttgacttgct ggttatggag aacctttttc acaatcaaaa catcttgaaa acctttgatc tcaagggcat tgagggtaga cgagtggcca aaacaaaaga aggggataaa gaggtaaagt cgacaacaac gcaatttgat gcggattggt tagaagggat gcagaaaggt ctcgttctgc ttcaaccaca cgccaaacgc atccttttgg atgcaatctc tctcgataca agatttcttt cctcacagtc tataatggat tactcactgt tactcggcgt tgacacccgc tcctctctgc taccttctcc aaatatctct gaggacaaat tggatcaaga gagggaaaat gggctgctga ttgtaggaat tgtggatgcc atcggaagct tcaacctctt caaaactatt gagagtagag ggaagatggt tatgaatcgc ggtggggacg tcaccgttat ccctcctgac caataccgcg aacgcttcga gaacgctatc cggcactatt tcgtggcttg ccctgataag tggtcgaaag tatctagaaa agtcgggggg tttaacgggt gtgaggtaag ctcagtatta taa

Details for CrneC.19314.a.B2.GE40907

HARVESTED ON:	12/20/2016
SEQUENCED ON:	12/27/2016
EXPECTED MW:	37kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMD NSLSTLSPRP HLHFTYTFAD KLRLSCTVYF ATAFDTLRRR CAIDKVLVQS LERTEVWDAQ GGKSKAGFWM TKDKRFIVKE LLSKWTVSDM HALLEISPAY FHHMAGTHNR ATALAKIVGF YTVTIKDSQC GQKRQLDLLV MENLFHNQNI LKTFDLKGIE GRRVAKTKEG DKEVKSTTTQ FDADWLEGMQ KGLVLLQPHA KRILLDAISL DTRFLSSQSI MDYSLLLGVD TRSSLLPSPN ISEDKLDQER ENGLLIVGIV DAIGSFNLFK TIESRGKMVM NRGGDVTVIP PDQYRERFEN AIR

Validated NT Sequence

gccggatagc gttctcgaag cgttcgcggt attggtcagg agggataacg gtgacgtccc caccgcgatt cataaccatc ttccctctac tctcaatagt tttgaagagg ttgaagcttc cgatggcatc cacaattcct acaatcagca gcccattttc cctctcttga tccaatttgt cctcagagat atttggagaa ggtagcagag aggagcgggt gtcaacgccg agtaacagtg agtaatccat tatagactgt gaggaaagaa atcttgtatc gagagagatt gcatccaaaa ggatgcgttt ggcgtgtggt tgaagcagaa cgagaccttt ctgcatccct tctaaccaat ccgcatcaaa ttgcgttgtt gtcgacttta cctctttatc cccttctttt gttttggcca ctcgtctacc ctcaatgccc ttgagatcaa aggttttcaa gatgttttga ttgtgaaaaa ggttctccat aaccagcaag tcaagttgcc gtttttggcc acattgggag tccttgatag tcactgtata aaatcctacg attttggcta aagctgtggc cctgttgtga gtccctgcca tgtggtggaa gtatgcaggt gaaatctcca gcaatgcgtg catatcactg acagtccact tgctcaacag ctccttcaca ataaacctct tatctttcgt catccaaaaa cccgctttac tcttcccacc ctgcgcgtcc catacttcgg tcctctcgag gctttggaca agcaccttgt ctatcgcaca ccttctcctt aatgtgtcga acgccgtcgc aaaataaact gtacaggata gccggagctt atcggcgaag gtgtaggtaa aatggagatg aggacggggt gaaagagtag ataaagaatt atccatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggata attctttatc tactctttca ccccgtcctc atctccattt tacctacacc ttcgccgata agctccggct atcctgtaca gtttattttg cgacggcgtt cgacacatta aggagaaggt gtgcgataga caaggtgctt gtccaaagcc tcgagaggac cgaagtatgg gacgcgcagg gtgggaagag taaagcgggt ttttggatga cgaaagataa gaggtttatt gtgaaggagc tgttgagcaa gtggactgtc agtgatatgc acgcattgct ggagatttca cctgcatact tccaccacat ggcagggact cacaacaggg ccacagcttt agccaaaatc gtaggatttt atacagtgac tatcaaggac tcccaatgtg gccaaaaacg gcaacttgac ttgctggtta tggagaacct ttttcacaat caaaacatct tgaaaacctt tgatctcaag ggcattgagg gtagacgagt ggccaaaaca aaagaagggg ataaagaggt aaagtcgaca acaacgcaat ttgatgcgga ttggttagaa gggatgcaga aaggtctcgt tctgcttcaa ccacacgcca aacgcatcct tttggatgca atctctctcg atacaagatt tctttcctca cagtctataa tggattactc actgttactc ggcgttgaca cccgctcctc tctgctacct tctccaaata tctctgagga caaattggat caagagaggg aaaatgggct gctgattgta ggaattgtgg atgccatcgg aagcttcaac ctcttcaaaa ctattgagag tagagggaag atggttatga atcgcggtgg ggacgtcacc gttatccctc ctgaccaata ccgcgaacgc ttcgagaacg ctatccggca ctatttcgtg gcttgccctg attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat