CrneC.19313.a
YjeF family protein (CKY1)
| CENTER ID: | CrneC.19313.a |
| ORGANISM: | Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487) |
| ASSOCIATED DISEASE: | Pneumonia-like illness |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrneC.19313.a.B1.GE40906 | full length | 1 | 346 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrneC.19313.a.B1.PS38377 | full length | 1 | 346 |
Structures
6EFW
DEPOSITED: 8/17/2018
DETERMINATION: XRay
CLONE: CrneC.19313.a.B1.GE40906
PROTEIN: CrneC.19313.a.B1.PS38377
6EFX
DEPOSITED: 8/17/2018
DETERMINATION: XRay
CLONE: CrneC.19313.a.B1.GE40906
PROTEIN: CrneC.19313.a.B1.PS38377
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_83552 |
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:CNAG_05097 |
| UniProt: | J9VIT7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASKQHAHIL SLARSMIPPL HPKLHKGQAG RIGVLGGSGD YSGAPYFSSM GAMRFGADLA HVICEPSAGA VIKTYSPDLI VHTILDPQKS REDIRSALKG VMSRLHVLII GPGLGRDDHM QSCAKIAFEL AKDMEQMGVV VDADGLWLVQ NEPKVVMDWP GVPRIILTPN VMEFKRLCDT MKINASGPHT SLCPQLATAL GNATIIQKGP SDIISNGLKI PFALLSDESE EEQNYLEVKV EGGLKRVGGQ GDILSGSTGV LLAWGSEWVR GTYEHVGHPP PQDKAIKENI PVLAAYGAST FNRTVSKRGF QKKGRSMVTG DLVDMVGEVY EEVFGNPGEV EGRGKL
NT Sequence
atggcatcaa agcagcacgc ccacatcctc tccctcgcga ggagcatgat ccctcctctc cacccaaagc tccataaggg tcaagccggt cgaatcggtg tacttggtgg atcaggggac tattcaggag cgccctactt ttcttccatg ggtgccatgc gctttggagc tgatttagcg catgtcattt gtgaaccttc tgctggagcc gttatcaaga cttactcccc tgatttgatc gtccacacca tcctcgaccc tcaaaagtct cgagaggaca tcaggtccgc cctcaagggc gtcatgtctc gcttgcatgt actcattatt ggccctggcc taggaaggga cgaccacatg cagagctgtg ccaagattgc ctttgagttg gccaaggaca tggagcagat gggggttgtt gtcgacgccg atggattgtg gcttgtccag aatgagccga aggtagtgat ggactggccc ggtgtccctc ggatcatcct tactcctaat gtcatggaat tcaagcgcct ttgtgacacc atgaaaatta acgcctctgg cccccatacc tcattgtgtc ctcagcttgc tactgctctc ggcaatgcca ccatcattca gaagggtcct agcgatatca tctccaacgg tctgaagatt ccatttgccc ttctttctga tgagtcagaa gaagagcaga attatttgga agtcaaggtc gaagggggct tgaagcgggt cggcggccag ggagatatct tgagtggtag taccggtgtt cttctcgctt ggggtagcga atgggttcga ggaacttacg aacatgtggg tcatcctcct cctcaagaca aggctattaa agagaacatc cctgtccttg cggcatatgg cgcttccacg ttcaaccgaa ccgtctcaaa acgaggcttc cagaaaaagg gcagaagtat ggtcactggt gatctcgttg acatggttgg tgaagtttat gaggaggttt tcgggaatcc aggggaagtt gagggccgag gtaaacttta g
Details for CrneC.19313.a.B1.GE40906
| HARVESTED ON: | 12/20/2016 |
| SEQUENCED ON: | 12/27/2016 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMA SKQHAHILSL ARSMIPPLHP KLHKGQAGRI GVLGGSGDYS GAPYFSSMGA MRFGADLAHV ICEPSAGAVI KTYSPDLIVH TILDPQKSRE DIRSALKGVM SRLHVLIIGP GLGRDDHMQS CAKIAFELAK DMEQMGVVVD ADGLWLVQNE PKVVMDWPGV PRIILTPNVM EFKRLCDTMK INASGPHTSL CPQLATALGN ATIIQKGPSD IISNGLKIPF ALLSDESEEE QNYLEVKVEG GLKRVGGQGD ILSGSTGVLL AWGSEWVRGT YEHVGHPPPQ DKAIKENIPV LAAYGASTFN RTVSKRGFQK KGRSMVTGDL VDMVGEVYEE VFGNPG
Validated NT Sequence
ttcccctgga ttcccgaaaa cctcctcata aacttcacca accatgtcaa cgagatcacc agtgaccata cttctgccct ttttctggaa gcctcgtttt gagacggttc ggttgaacgt ggaagcgcca tatgccgcaa ggacagggat gttctcttta atagccttgt cttgaggagg aggatgaccc acatgttcgt aagttcctcg aacccattcg ctaccccaag cgagaagaac accggtacta ccactcaaga tatctccctg gccgccgacc cgcttcaagc ccccttcgac cttgacttcc aaataattct gctcttcttc tgactcatca gaaagaaggg caaatggaat cttcagaccg ttggagatga tatcgctagg acccttctga atgatggtgg cattgccgag agcagtagca agctgaggac acaatgaggt atgggggcca gaggcgttaa ttttcatggt gtcacaaagg cgcttgaatt ccatgacatt aggagtaagg atgatccgag ggacaccggg ccagtccatc actaccttcg gctcattctg gacaagccac aatccatcgg cgtcgacaac aacccccatc tgctccatgt ccttggccaa ctcaaaggca atcttggcac agctctgcat gtggtcgtcc cttcctaggc cagggccaat aatgagtaca tgcaagcgag acatgacgcc cttgagggcg gacctgatgt cctctcgaga cttttgaggg tcgaggatgg tgtggacgat caaatcaggg gagtaagtct tgataacggc tccagcagaa ggttcacaaa tgacatgcgc taaatcagct ccaaagcgca tggcacccat ggaagaaaag tagggcgctc ctgaatagtc ccctgatcca ccaagtacac cgattcgacc ggcttgaccc ttatggagct ttgggtggag aggagggatc atgctcctcg cgagggagag gatgtgggcg tgctgctttg atgccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMA SKQHAHILSL ARSMIPPLHP KLHKGQAGRI GVLGGSGDYS GAPYFSSMGA MRFGADLAHV ICEPSAGAVI KTYSPDLIVH TILDPQKSRE DIRSALKGVM SRLHVLIIGP GLGRDDHMQS CAKIAFELAK DMEQMGVVVD ADGLWLVQNE PKVVMDWPGV PRIILTPNVM EFKRLCDTMK INASGPHTSL CPQLATALGN ATIIQKGPSD IISNGLKIPF ALLSDESEEE QNYLEVKVEG GLKRVGGQGD ILSGSTGVLL AWGSEWVRGT YEHVGHPPPQ DKAIKENIPV LAAYGASTFN RTVSKRGFQK KGRSMVTGDL VDMVGEVYEE VFGNPGEVEG RGKL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcat caaagcagca cgcccacatc ctctccctcg cgaggagcat gatccctcct ctccacccaa agctccataa gggtcaagcc ggtcgaatcg gtgtacttgg tggatcaggg gactattcag gagcgcccta cttttcttcc atgggtgcca tgcgctttgg agctgattta gcgcatgtca tttgtgaacc ttctgctgga gccgttatca agacttactc ccctgatttg atcgtccaca ccatcctcga ccctcaaaag tctcgagagg acatcaggtc cgccctcaag ggcgtcatgt ctcgcttgca tgtactcatt attggccctg gcctaggaag ggacgaccac atgcagagct gtgccaagat tgcctttgag ttggccaagg acatggagca gatgggggtt gttgtcgacg ccgatggatt gtggcttgtc cagaatgagc cgaaggtagt gatggactgg cccggtgtcc ctcggatcat ccttactcct aatgtcatgg aattcaagcg cctttgtgac accatgaaaa ttaacgcctc tggcccccat acctcattgt gtcctcagct tgctactgct ctcggcaatg ccaccatcat tcagaagggt cctagcgata tcatctccaa cggtctgaag attccatttg cccttctttc tgatgagtca gaagaagagc agaattattt ggaagtcaag gtcgaagggg gcttgaagcg ggtcggcggc cagggagata tcttgagtgg tagtaccggt gttcttctcg cttggggtag cgaatgggtt cgaggaactt acgaacatgt gggtcatcct cctcctcaag acaaggctat taaagagaac atccctgtcc ttgcggcata tggcgcttcc acgttcaacc gaaccgtctc aaaacgaggc ttccagaaaa agggcagaag tatggtcact ggtgatctcg ttgacatggt tggtgaagtt tatgaggagg ttttcgggaa tccaggggaa gttgagggcc gaggtaaact ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for CrneC.19313.a.B1.PS38377
| PURIFICATION DATe: | 10/31/2017 |
| CONCENTRATION: | 20.52mg/ml |
| OBSERVED MW: | 38kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA SKQHAHILSL ARSMIPPLHP KLHKGQAGRI GVLGGSGDYS GAPYFSSMGA MRFGADLAHV ICEPSAGAVI KTYSPDLIVH TILDPQKSRE DIRSALKGVM SRLHVLIIGP GLGRDDHMQS CAKIAFELAK DMEQMGVVVD ADGLWLVQNE PKVVMDWPGV PRIILTPNVM EFKRLCDTMK INASGPHTSL CPQLATALGN ATIIQKGPSD IISNGLKIPF ALLSDESEEE QNYLEVKVEG GLKRVGGQGD ILSGSTGVLL AWGSEWVRGT YEHVGHPPPQ DKAIKENIPV LAAYGASTFN RTVSKRGFQK KGRSMVTGDL VDMVGEVYEE VFGNPG
Validated NT Sequence
ttcccctgga ttcccgaaaa cctcctcata aacttcacca accatgtcaa cgagatcacc agtgaccata cttctgccct ttttctggaa gcctcgtttt gagacggttc ggttgaacgt ggaagcgcca tatgccgcaa ggacagggat gttctcttta atagccttgt cttgaggagg aggatgaccc acatgttcgt aagttcctcg aacccattcg ctaccccaag cgagaagaac accggtacta ccactcaaga tatctccctg gccgccgacc cgcttcaagc ccccttcgac cttgacttcc aaataattct gctcttcttc tgactcatca gaaagaaggg caaatggaat cttcagaccg ttggagatga tatcgctagg acccttctga atgatggtgg cattgccgag agcagtagca agctgaggac acaatgaggt atgggggcca gaggcgttaa ttttcatggt gtcacaaagg cgcttgaatt ccatgacatt aggagtaagg atgatccgag ggacaccggg ccagtccatc actaccttcg gctcattctg gacaagccac aatccatcgg cgtcgacaac aacccccatc tgctccatgt ccttggccaa ctcaaaggca atcttggcac agctctgcat gtggtcgtcc cttcctaggc cagggccaat aatgagtaca tgcaagcgag acatgacgcc cttgagggcg gacctgatgt cctctcgaga cttttgaggg tcgaggatgg tgtggacgat caaatcaggg gagtaagtct tgataacggc tccagcagaa ggttcacaaa tgacatgcgc taaatcagct ccaaagcgca tggcacccat ggaagaaaag tagggcgctc ctgaatagtc ccctgatcca ccaagtacac cgattcgacc ggcttgaccc ttatggagct ttgggtggag aggagggatc atgctcctcg cgagggagag gatgtgggcg tgctgctttg atgccatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHMA SKQHAHILSL ARSMIPPLHP KLHKGQAGRI GVLGGSGDYS GAPYFSSMGA MRFGADLAHV ICEPSAGAVI KTYSPDLIVH TILDPQKSRE DIRSALKGVM SRLHVLIIGP GLGRDDHMQS CAKIAFELAK DMEQMGVVVD ADGLWLVQNE PKVVMDWPGV PRIILTPNVM EFKRLCDTMK INASGPHTSL CPQLATALGN ATIIQKGPSD IISNGLKIPF ALLSDESEEE QNYLEVKVEG GLKRVGGQGD ILSGSTGVLL AWGSEWVRGT YEHVGHPPPQ DKAIKENIPV LAAYGASTFN RTVSKRGFQK KGRSMVTGDL VDMVGEVYEE VFGNPGEVEG RGKL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAT CAAAGCAGCA CGCCCACATC CTCTCCCTCG CGAGGAGCAT GATCCCTCCT CTCCACCCAA AGCTCCATAA GGGTCAAGCC GGTCGAATCG GTGTACTTGG TGGATCAGGG GACTATTCAG GAGCGCCCTA CTTTTCTTCC ATGGGTGCCA TGCGCTTTGG AGCTGATTTA GCGCATGTCA TTTGTGAACC TTCTGCTGGA GCCGTTATCA AGACTTACTC CCCTGATTTG ATCGTCCACA CCATCCTCGA CCCTCAAAAG TCTCGAGAGG ACATCAGGTC CGCCCTCAAG GGCGTCATGT CTCGCTTGCA TGTACTCATT ATTGGCCCTG GCCTAGGAAG GGACGACCAC ATGCAGAGCT GTGCCAAGAT TGCCTTTGAG TTGGCCAAGG ACATGGAGCA GATGGGGGTT GTTGTCGACG CCGATGGATT GTGGCTTGTC CAGAATGAGC CGAAGGTAGT GATGGACTGG CCCGGTGTCC CTCGGATCAT CCTTACTCCT AATGTCATGG AATTCAAGCG CCTTTGTGAC ACCATGAAAA TTAACGCCTC TGGCCCCCAT ACCTCATTGT GTCCTCAGCT TGCTACTGCT CTCGGCAATG CCACCATCAT TCAGAAGGGT CCTAGCGATA TCATCTCCAA CGGTCTGAAG ATTCCATTTG CCCTTCTTTC TGATGAGTCA GAAGAAGAGC AGAATTATTT GGAAGTCAAG GTCGAAGGGG GCTTGAAGCG GGTCGGCGGC CAGGGAGATA TCTTGAGTGG TAGTACCGGT GTTCTTCTCG CTTGGGGTAG CGAATGGGTT CGAGGAACTT ACGAACATGT GGGTCATCCT CCTCCTCAAG ACAAGGCTAT TAAAGAGAAC ATCCCTGTCC TTGCGGCATA TGGCGCTTCC ACGTTCAACC GAACCGTCTC AAAACGAGGC TTCCAGAAAA AGGGCAGAAG TATGGTCACT GGTGATCTCG TTGACATGGT TGGTGAAGTT TATGAGGAGG TTTTCGGGAA TCCAGGGGAA GTTGAGGGCC GAGGTAAACT TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT