SSGCID: Target Page

CrneC.19309.a

Xylulokinase

CENTER ID:	CrneC.19309.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.19309.a.B1.GE40835	full length		1	626

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_05243
UniProt:	J9VJV0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSPLFLGLD ASTQSLKASL LSVNLDVVAE CAVHFDSDLP QFGTKGGVHF GSDGQVHSPV MMLAEAMDLL FDKIKIAGWK VDDIRGVAAA GQQHASVYWS RTSSKLLASL NPSLPLSSQL AEAFSRPIIP NWQDSSTTAE CQALDAAVGG PAALAQLTGS RAYERFTGAQ IMRFKRVDPV AYDQTDRIGL VSNTVTTLLC LDGEVKGIDE SDACGMNLWT MNRKQRGWNQ ELLKAIAGDD GAAELSRKLG TVETDGGRVV GHIGKWFVDR YGFNSECCVF PGTGDNPATF LSLTLRESEG LVSLGTSDVV LISTSTYHPD PEYHAFFHPA QIAPPSEQDE QNRQGAEPLR YFNMIVYKNG SLTRQHVRDL YFDGSWDKFN AAIEELRPKS VIDLPSRTAF WWLLPDIVPH GAHGIYKYIT DPTAGTLFEV PAAKKVDRFP DIRQEALALL ESQLFNYRSR VSSILDDSST TYDPSSPNVD AFLPRLTKVY ATGGASANRT ILSLMADVLS TKVCKNVEYL DGKWKDADWN ACSVGVAYKA RWGWERVIAA DGDERKWVGF DTVIRECREA RKKIRGDEGK GLELEEEGIR IIASPGPGSR AYERRVEWWR ELERKALEEQ RAEKTA

NT Sequence

atgtcgtctc ctctgttcct cggcctcgac gcatccacac aatccctcaa agcttctcta ctctctgtca acctggatgt agtggccgaa tgtgccgtcc actttgactc ggacttgcct caattcggta caaaaggagg tgtacatttt ggctcagatg gtcaagttca ttcgccggta atgatgctgg ccgaagcgat ggatttgctt tttgacaaga tcaaaatcgc cggctggaaa gttgacgata ttcgaggagt cgcagccgcc ggacagcaac acgcttcagt ttattggtct aggacatcca gtaaacttct tgcttccctc aacccctcac tgcccttatc ttcacaactc gccgaggcct tttctagacc cattatcccc aactggcaag attcttccac caccgccgaa tgtcaggcac tggacgccgc cgttggtggc ccagctgctc ttgcacagct tactggttct cgagcatatg agagattcac tggcgctcaa atcatgcgtt tcaaacgggt cgatcctgtc gcatacgatc agactgaccg gatcggtctt gtcagtaaca cggtgactac tttgctctgc ttggatggcg aagtcaaggg gatcgacgaa agtgatgctt gcgggatgaa tctgtggact atgaatagaa aacagcgagg gtggaatcag gaattgctga aggcaatcgc tggtgatgat ggagcagcag agctttccag gaaactgggg acagtggaga cggatggagg tagggttgtg gggcatatag gcaaatggtt tgttgatcgg tacgggttca attcggaatg ctgtgtgttc cctggcaccg gggacaatcc agctacattc ttgagtttga cgttacgcga aagcgaaggt ctagtctctc ttggcacttc tgatgttgtc ctcatttcca caagcaccta ccaccctgat cccgagtacc acgcattctt ccacccggcc cagatagcac ctcctagtga gcaagacgaa caaaacagac aaggagcaga acctctgaga tactttaata tgatcgttta taagaatggc tctcttaccc gacaacacgt gcgagacctc tattttgacg gatcatggga caagtttaat gcggccattg aggaactacg ccccaagtca gtcatcgacc ttccttctcg tacagccttc tggtggcttc tcccagatat tgtaccgcat ggtgcccatg gtatctacaa atacataacc gaccctactg ctgggactct tttcgaagta ccagctgcta agaaagtcga ccgattccca gacatcaggc aagaagccct tgctttgctc gaatcccaac ttttcaatta ccgttcccgc gtatcctcca tccttgacga ctcttccacc acgtacgacc cttcctcccc caatgtcgat gctttcctcc ctcgtctgac caaggtatac gccacaggcg gagccagcgc taaccgtact atcttgagtt tgatggcgga tgtattatcg acaaaggtgt gtaagaatgt agagtatctt gatgggaaat ggaaagatgc ggattggaat gcctgtagtg tcggagtggc gtacaaggct cgttggggat gggagcgagt gatagcagct gatggggatg agaggaagtg ggttgggttt gatacggtaa tcagggaatg tagagaggcg agaaagaaaa ttcgaggtga tgaagggaag ggtttggaat tggaagagga gggtatcagg attatagcca gtccaggccc tgggtcaagg gcgtatgaga gaagggttga atggtggaga gagctggaac gcaaagcgtt ggaggaacag cgagctgaaa agaccgcgtg a

Details for CrneC.19309.a.B1.GE40835

HARVESTED ON:	12/5/2016
SEQUENCED ON:	12/6/2016
EXPECTED MW:	70kDa
OBSERVED MW:	70kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	43

Validated AA Sequence

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS SPLFLGLDAS TQSLKASLLS VNLDVVAECA VHFDSDLPQF GTKGGVHFGS DGQVHSPVMM LAEAMDLLFD KIKIAGWKVD DIRGVAAAGQ QHASVYWSRT SSKLLASLNP SLPLSSQLAE AFSRPIIPNW QDSSTTAECQ ALDAAVGGPA ALAQLTGSRA YERFTGAQIM RFKRVDPVAY DQTDRIGLVS NTVTTLLCLD GEVKGIDESD ACGMNLWTMN RKQRGWNQEL LKAIAGDDGA AELSRKLGTV ETDGGRVVGH IGKWFVDRYG FNSECCVFPG TGDNPATFLS LTLRESEGLV SLGTSDVVLI STSTYHPDPE YHAFFHPAQI APPSEQDEQN RQGAEPLRYF NMIVYKNGSL TRQHVRDLYF DGSWDKFNAA IEELRPKSVI DLPSRTAFWW LLPDIVPHGA HGIYKYITDP TAGTLFEVPA AKKVDRFPDI RQEALALLES QLFNYRSRVS SILDDSSTTY DPSSPNVDAF LPRLTKVYAT GGASANRTIL SLMADVLSTK VCKNVEYLDG KWKDADWNAC SVGVAYKARW GWERVIAADG DERKWVGFDT VIRECREARK KIRGDEGKGL ELEEEGIRII ASPGPGSRAY ERRVEWWREL ERKALEEQRA EKTA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgt ctcctctgtt cctcggcctc gacgcatcca cacaatccct caaagcttct ctactctctg tcaacctgga tgtagtggcc gaatgtgccg tccactttga ctcggacttg cctcaattcg gtacaaaagg aggtgtacat tttggctcag atggtcaagt tcattcgccg gtaatgatgc tggccgaagc gatggatttg ctttttgaca agatcaaaat cgccggctgg aaagttgacg atattcgagg agtcgcagcc gccggacagc aacacgcttc agtttattgg tctaggacat ccagtaaact tcttgcttcc ctcaacccct cactgccctt atcttcacaa ctcgccgagg ccttttctag acccattatc cccaactggc aagattcttc caccaccgcc gaatgtcagg cactggacgc cgccgttggt ggcccagctg ctcttgcaca gcttactggt tctcgagcat atgagagatt cactggcgct caaatcatgc gtttcaaacg ggtcgatcct gtcgcatacg atcagactga ccggatcggt cttgtcagta acacggtgac tactttgctc tgcttggatg gcgaagtcaa ggggatcgac gaaagtgatg cttgcgggat gaatctgtgg actatgaata gaaaacagcg agggtggaat caggaattgc tgaaggcaat cgctggtgat gatggagcag cagagctttc caggaaactg gggacagtgg agacggatgg aggtagggtt gtggggcata taggcaaatg gtttgttgat cggtacgggt tcaattcgga atgctgtgtg ttccctggca ccggggacaa tccagctaca ttcttgagtt tgacgttacg cgaaagcgaa ggtctagtct ctcttggcac ttctgatgtt gtcctcattt ccacaagcac ctaccaccct gatcccgagt accacgcatt cttccacccg gcccagatag cacctcctag tgagcaagac gaacaaaaca gacaaggagc agaacctctg agatacttta atatgatcgt ttataagaat ggctctctta cccgacaaca cgtgcgagac ctctattttg acggatcatg ggacaagttt aatgcggcca ttgaggaact acgccccaag tcagtcatcg accttccttc tcgtacagcc ttctggtggc ttctcccaga tattgtaccg catggtgccc atggtatcta caaatacata accgacccta ctgctgggac tcttttcgaa gtaccagctg ctaagaaagt cgaccgattc ccagacatca ggcaagaagc ccttgctttg ctcgaatccc aacttttcaa ttaccgttcc cgcgtatcct ccatccttga cgactcttcc accacgtacg acccttcctc ccccaatgtc gatgctttcc tccctcgtct gaccaaggta tacgccacag gcggagccag cgctaaccgt actatcttga gtttgatggc ggatgtatta tcgacaaagg tgtgtaagaa tgtagagtat cttgatggga aatggaaaga tgcggattgg aatgcctgta gtgtcggagt ggcgtacaag gctcgttggg gatgggagcg agtgatagca gctgatgggg atgagaggaa gtgggttggg tttgatacgg taatcaggga atgtagagag gcgagaaaga aaattcgagg tgatgaaggg aagggtttgg aattggaaga ggagggtatc aggattatag ccagtccagg ccctgggtca agggcgtatg agagaagggt tgaatggtgg agagagctgg aacgcaaagc gttggaggaa cagcgagctg aaaagaccgc gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat