CrneC.19307.a

Galactokinase, variant

CENTER ID: CrneC.19307.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19307.a.B2.GE40834 galactokinase domain 25 397
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_03946
UniProt: T2BPC9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAKVPITVF NSLQEIYPSA SAVLREGQRW NTLLARFQEQ FGEAPAYIVR APGRVNVLGE HIDYSLFPVL PAAIEQDILF AFCPTRPVAG SNPTVRLENY DRKYSYPGCS FSLVPGEKGW DVGLDARGGW DKYVRAALLE CLDELFPVGK GDGRQEAVGM DVLVSGSIPP GSGLSSSAAM VVGSVIMFLV ANNLAAGKTK ADVVQLAINS EHRMGLRTGG MDQSASALAL PNNLLHLSFH PGLLPAPLPL PGNVSLVITN SLAPHSLTGS APEEYNLRVI EILIATRLIL HHWKLESHFN DNPRPWLREV LGAWVGEKGH MGWEKEGEVM EKALGDIEWI KRDGGWSREE MVKYSGMNEE EFKKSYLDFL EIRAEKFHLY ERLHHTLTES LRVHKFQPTS SASSASYSMH LTLPCEIRMT APTLSWIRCK SCV
NT Sequence
atgtctgcca aggtccccat caccgtcttc aactctctgc aagaaatata tccatctgct tctgccgttc ttcgcgaagg tcaacgatgg aacacccttc tcgctcggtt ccaagaacag tttggcgagg ctccagccta catcgttcgt gcccctgggc gggtcaatgt acttggagag cacatcgatt actctctttt ccccgtactt cctgcggcta ttgagcaaga tatccttttc gctttttgcc ctacgcgtcc tgttgccggt tcgaatccaa cagtacggtt agaaaattat gacagaaagt acagttaccc cggctgctct ttctcacttg tccctggaga aaaaggatgg gatgtaggtc tcgatgccag aggaggatgg gacaagtacg ttagggctgc tttattggag tgcttggatg agcttttccc cgttggcaag ggggatggaa gacaagaggc agtaggaatg gatgtgcttg tttctggcag tatacctcct ggctctggat tgagtagctc cgcagcaatg gtcgttggct ctgtcatcat gttcctagtt gccaacaatt tggcggcagg taaaacgaaa gcggacgttg tgcaactcgc catcaattcc gagcatcgca tgggcttgcg cacaggtggc atggaccaat ctgcctctgc ccttgctttg ccgaacaacc ttttacacct atcttttcac ccaggcttgt tgcctgcacc tttacctctt cccggtaacg tatcgctggt catcacaaac tctcttgccc cgcattcttt gacaggttct gcgccggaag agtacaactt gcgagtcatt gagattctca tcgccacgcg tttgatctta catcactgga agttggagtc acatttcaac gataacccta gaccatggtt gagagaagtc ttgggcgctt gggtaggtga gaagggtcat atgggatggg agaaggaagg agaagtgatg gagaaggctc ttggtgatat cgaatggatc aaaagagatg gagggtggag tagggaagag atggtcaaat attcgggcat gaatgaagaa gagttcaaga aaagttacct tgacttcctc gagattcgag cagaaaagtt ccacctctat gaacgcttgc atcataccct caccgaatct ttacgcgttc acaaattcca accgacatcc tcagccagct cggcaagtta ttcgatgcat ctcacgcttc catgcgagat acgtatgact gcacccaccc tctcgtggat tcgctgcaag agctgtgttt aa
Details for CrneC.19307.a.B2.GE40834
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMR EGQRWNTLLA RFQEQFGEAP AYIVRAPGRV NVLGEHIDYS LFPVLPAAIE QDILFAFCPT RPVAGSNPTV RLENYDRKYS YPGCSFSLVP GEKGWDVGLD ARGGWDKYVR AALLECLDEL FPVGKGDGRQ EAVGMDVLVS GSIPPGSGLS SSAAMVVGSV IMFLVANNLA AGKTKADVVQ LAINSEHRMG LRTGGMDQSA SALALPNNLL HLSFHPGLLP APLPLPGNVS LVITNSLAPH SLTGSAPEEY NLRVIEILIA TRLILHHWKL ESHFNDNPRP WLREVLGAWV GEKGHMGWEK EGEVIEKALG DIEWIKRDGG WSREEMVKYS GMNEEEFKKS YLDFLEIRAE KFHLYERLHH TLTE
Validated NT Sequence
attcggtgag ggtatgatgc aagcgttcat agaggtggaa cttttctgct cgaatctcga ggaagtcaag gtaacttttc ttgaactctt cttcattcat gcccgaatat ttgaccatct cttccctact ccaccctcca tctcttttga tccattcgat atcaccaaga gccttctcta tcacttctcc ttccttctcc catcccatat gacccttctc acctacccaa gcgcccaaga cttctctcaa ccatggtcta gggttatcgt tgaaatgtga ctccaacttc cagtgatgta agatcaaacg cgtggcgatg agaatctcaa tgactcgcaa gttgtactct tccggcgcag aacctgtcaa agaatgcggg gcaagagagt ttgtgatgac cagcgatacg ttaccgggaa gaggtaaagg tgcaggcaac aagcctgggt gaaaagatag gtgtaaaagg ttgttcggca aagcaagggc agaggcagat tggtccatgc cacctgtgcg caagcccatg cgatgctcgg aattgatggc gagttgcaca acgtccgctt tcgttttacc tgccgccaaa ttgttggcaa ctaggaacat gatgacagag ccaacgacca ttgctgcgga gctactcaat ccagagccag gaggtatact gccagaaaca agcacatcca ttcctactgc ctcttgtctt ccatccccct tgccaacggg gaaaagctca tccaagcact ccaataaagc agccctaacg tacttgtccc atcctcctct ggcatcgaga cctacatccc atcctttttc tccagggaca agtgagaaag agcagccggg gtaactgtac tttctgtcat aattttctaa ccgtactgtt ggattcgaac cggcaacagg acgcgtaggg caaaaagcga aaaggatatc ttgctcaata gccgcaggaa gtacggggaa aagagagtaa tcgatgtgct ctccaagtac attgacccgc ccaggggcac gaacgatgta ggctggagcc tcgccaaact gttcttggaa ccgagcgaga agggtgttcc atcgttgacc ttcgcgcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHRE GQRWNTLLAR FQEQFGEAPA YIVRAPGRVN VLGEHIDYSL FPVLPAAIEQ DILFAFCPTR PVAGSNPTVR LENYDRKYSY PGCSFSLVPG EKGWDVGLDA RGGWDKYVRA ALLECLDELF PVGKGDGRQE AVGMDVLVSG SIPPGSGLSS SAAMVVGSVI MFLVANNLAA GKTKADVVQL AINSEHRMGL RTGGMDQSAS ALALPNNLLH LSFHPGLLPA PLPLPGNVSL VITNSLAPHS LTGSAPEEYN LRVIEILIAT RLILHHWKLE SHFNDNPRPW LREVLGAWVG EKGHMGWEKE GEVMEKALGD IEWIKRDGGW SREEMVKYSG MNEEEFKKSY LDFLEIRAEK FHLYERLHHT LTESLRVHKF Q
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgcgaag gtcaacgatg gaacaccctt ctcgctcggt tccaagaaca gtttggcgag gctccagcct acatcgttcg tgcccctggg cgggtcaatg tacttggaga gcacatcgat tactctcttt tccccgtact tcctgcggct attgagcaag atatcctttt cgctttttgc cctacgcgtc ctgttgccgg ttcgaatcca acagtacggt tagaaaatta tgacagaaag tacagttacc ccggctgctc tttctcactt gtccctggag aaaaaggatg ggatgtaggt ctcgatgcca gaggaggatg ggacaagtac gttagggctg ctttattgga gtgcttggat gagcttttcc ccgttggcaa gggggatgga agacaagagg cagtaggaat ggatgtgctt gtttctggca gtatacctcc tggctctgga ttgagtagct ccgcagcaat ggtcgttggc tctgtcatca tgttcctagt tgccaacaat ttggcggcag gtaaaacgaa agcggacgtt gtgcaactcg ccatcaattc cgagcatcgc atgggcttgc gcacaggtgg catggaccaa tctgcctctg cccttgcttt gccgaacaac cttttacacc tatcttttca cccaggcttg ttgcctgcac ctttacctct tcccggtaac gtatcgctgg tcatcacaaa ctctcttgcc ccgcattctt tgacaggttc tgcgccggaa gagtacaact tgcgagtcat tgagattctc atcgccacgc gtttgatctt acatcactgg aagttggagt cacatttcaa cgataaccct agaccatggt tgagagaagt cttgggcgct tgggtaggtg agaagggtca tatgggatgg gagaaggaag gagaagtgat ggagaaggct cttggtgata tcgaatggat caaaagagat ggagggtgga gtagggaaga gatggtcaaa tattcgggca tgaatgaaga agagttcaag aaaagttacc ttgacttcct cgagattcga gcagaaaagt tccacctcta tgaacgcttg catcataccc tcaccgaatc tttacgcgtt cacaaattcc aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat