CrneC.19306.a

VPS15 protein kinase

CENTER ID: CrneC.19306.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19306.a.B3.GE40833 protein kinase 66 430
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_02680
UniProt: J9VHV7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPAAGYHVVS ASSISIKTPT PHIVGKEHRT HETTRFHGIR PAPMGNISSM ARASTALDSY VAELGNDISY DKSLSSSRFL KTILARHSFG PIVLKIFIKP DATMSLRVIQ RRLKLERDIL SELANVNTYQ TFVETEKAGY LIRQWIGSNL YDRVSQQPYL ANIEKKWIAF QILTTLRDAR NRKVAHGDIK SENILLTSSL SLLLTDFSSS FKPTYLPLDD PSDFSFFFDT SGRRACYIAP ERFYTSDSKV ADRKRASESE GGEWFARRDG KITEEMDVFS TGCVLAEMWA DGRTVFNLSE LYAYRDGALN LNGMLENIHD VDVREMIAQM LSREPSKRPS FDHILSKYRE TIFPEYFYTF LQDYINSLNE KAEDSSFRDD VEGGFLQHSA GQSGAKIDRL LDEWEGIATY LGECPTDNGP ALLILNVITS SVRHCLFPSS RLHALRLFLN LLPYLLDEDK IDRIIPYIVE LLSDDMPHVR AEACRTLIQV VESVTSVTPQ NATFIPEYLL PQMKHLTTDS DIFVRITYAQ ALVKLANAAM TMLEMSQAAK DGASVHGTES SGIVEPDYDA MLQEIQAAVE EQATTLLVDP AAAVKRGILS SISDLCLFFG RQKSNEIVLS HIMTYLNDKD WMLRLAFFDG IVGVGAFIGL RAVEEYVLPL MFQALADPEE AVMARVIGSL TSLASLGLLA RMRLWDTFFA VKGFMFHPNV WIRQGAVGLV AAVAKNLPSS DVWCILYPAI RPMLHSDVKE MDEAALMSAL LPPLARSTLI AARVAALQNT PPGFWDIPPL ASSTKVDLIN SQKLPPNNPH RLRDSGISAL DEKKIEAMKE HIVKQAHAIK AREATDQNPP PEASLVNSKS VSLTDLGVTP QTIFISPRTI GVDAAKVELR RFRPGLEPES RRTSFTTNRS GRGPVDNPLD EIRRKLAALE PLSRPETPTA KEKATTATTT TTSHTSQPSV DTGASPSESN LSSTLDITAM TRSGKKKKMD SKAAPAVGAS HTNAVGTTTI HNEPISGRTT PNATALSQIT LSGANTPKGQ PAAPYTSSYE GHDPGVRAFL EQVDLDNYRE PLLDFGPRVG ANHRKRNPRV NKTSSSSSAS ASSSPTNVTM IAHLTQHEGA ITGIITSPDS VFFASSSEDG QVLIWDAARL ERSVTAKPRL VYKMEAPIVS MCGIDNTYCL AVASEDGQVH VLRVHTGGGS GGSSTRYSKA ECIRTWNTEE SDGHVRFVSH LQDSTLLLLT SMSVIATLDI RSMEIKQRFQ HPPELGVITA YCLSTHWLIL GTSIGVLSLW DLRFGLLLKS WKAGGGVTSC RIHPSRGRGR WIMVSVSPTW PYALPGTSTN KSEESQPSKP IVEVYDIESS KLVEVYEIRS RRSSKPSETV PEPIEVIPNK AALIAELARS TPSLPSLVPI DDFDATNPLP TPPSAVLDLM VGQGWTSLGR SDESILMSVP ELGSSDKNGE GQGGPGWMIT AGEDRIVRYW DLIKAQDGFV VCGSQKEKDV SFRRMPEGSP PTMFYTLPNV HRQGQGPSRD KERQPLQPHY DAICKLGSLE TPFSSCIISA DRSGIIKVWR MEGMAGPLR
NT Sequence
atgcctgcgg cggggtacca tgttgtctcc gccagcagca tctccatcaa gacaccgacc ccccatattg tcggcaaaga acatcgtact cacgagacca ccagattcca tgggataagg cccgcaccaa tgggaaacat atcttccatg gccagagcct caacggcgct ggattcttac gttgccgaac ttggcaatga catctcatac gacaagagtt tgtcatcctc caggttcctc aagaccatct tagctcgcca tagtttcggg ccgatcgtcc tcaagatatt cattaaaccc gatgcaacca tgtcacttcg ggttatacag cgaagattaa agttggagag ggatattctt tcagaacttg cgaatgtgaa cacatatcag acatttgtgg agacggagaa ggccggatat cttatcaggc aatggatagg atcaaacttg tatgaccgtg tcagtcaaca gccgtacctt gccaatatag aaaagaagtg gatcgccttc caaatattaa ccacccttcg agatgctcgc aatcgcaagg ttgctcacgg cgatatcaag tctgaaaaca tactcctcac ttcctccctc tctcttcttc tgacggactt ttcatcctcc ttcaaaccaa cgtaccttcc acttgacgac ccctccgact tttcattttt cttcgatacc tctgggcgac gcgcatgcta catcgcaccg gaacgatttt acacctcaga ctccaaagtg gctgatcgga aaagggcatc cgagagcgaa ggtggggaat ggtttgcgag gagggatggg aagattacag aggaaatgga tgtctttagt actgggtgtg tgttggcaga aatgtgggct gatgggcgga cggtatttaa tctcagtgag ttgtatgcgt atcgcgatgg cgctttaaat ttgaatggaa tgctggaaaa tatccatgac gtcgatgtcc gagaaatgat tgcgcaaatg ctatcaagag aaccttccaa acgaccctca ttcgaccata tcctttcaaa gtaccgtgaa accatctttc ccgaatattt ttacactttt ttacaagatt atattaactc actcaacgaa aaggcggaag attctagttt tagggatgat gtggaagggg gattcttgca acattcagca ggacaaagtg gtgccaagat tgacaggtta ttagacgaat gggaagggat tgccacttat ctcggtgaat gcccaaccga taacggcccg gcactactca tcttgaacgt gatcacctct tccgttagac actgtctatt cccttcgtcc cgtctacacg ccctccgcct attcttaaat ctcctcccgt acctccttga cgaagacaaa atcgaccgca ttatacccta catcgtcgag ctcctctccg acgacatgcc gcatgtccgc gctgaggcgt gcaggaccct gattcaagtc gtcgagagcg tcacctcggt gactccacag aacgcgacgt tcatcccaga gtatctttta ccgcagatga agcacttgac gacggacagt gatatcttcg taaggatcac atatgcacag gctttggtca agttggcgaa tgcggcgatg acaatgttgg agatgagtca ggctgcgaaa gatggtgcgt cagttcatgg aactgagtct tctggtattg tcgagcccga ctacgatgcg atgcttcaag agattcaagc cgccgtcgag gaacaagcta caaccctcct cgtcgaccct gccgccgctg tcaagcgcgg aatcctctcc tccatttctg acctgtgcct gtttttcggt cgtcaaaagt caaacgaaat cgtgctcagc catattatga cttacttgaa tgataaagat tggatgcttc ggctggcgtt ctttgatggt atcgtgggtg tgggagcgtt tataggttta agagctgtgg aagaatacgt gcttccgctc atgtttcaag cgttagcgga ccctgaagaa gctgtaatgg caagggtgat cggctcgctg acaagcctcg catctctcgg cttacttgca cgtatgcgat tatgggatac ttttttcgct gtgaaagggt tcatgttcca cccaaatgta tggattcgcc aaggcgcggt gggtcttgtc gcagctgtgg caaaaaacct cccgtcttcg gatgtatggt gtattctcta ccctgcgatt aggcctatgc tccattcgga tgtcaaggag atggatgaag cggcactaat gtcggctctc ttacctcctc tggcgagaag cactttgata gctgcaaggg ttgcggcgct gcaaaacact ccacctggct tttgggatat ccccccgcta gcatcatcga ccaaggttga tcttatcaat tctcagaaat taccaccaaa taaccctcat cggctgagag atagcggtat ctctgcattg gacgagaaaa agatcgaggc gatgaaggag catattgtta aacaagcgca tgctatcaaa gcgcgggaag cgaccgatca gaaccctccg ccggaggcat cacttgtcaa ttccaaatct gtctccctga ccgacttggg tgtaacgcct caaactatct tcatcagtcc ccgtacaatc ggcgtcgatg cggccaaagt cgaactccga cgtttccgtc caggtctcga gcctgaaagt cgccgaacgt cgtttactac taatcgaagc ggtcgcggtc ctgttgataa ccccttagac gagattcgca gaaaactggc cgcccttgaa ccgctctcaa ggcctgagac acctaccgca aaagaaaaag cgactacagc aacgacgacg actacaagtc atactagtca gccgtctgta gatacaggcg cgagcccgtc agagtccaat ctatcgtcga cactggatat cactgccatg acaagatctg ggaagaagaa aaagatggat tcaaaagctg ccccagcagt aggagcatcg cataccaatg cggtgggcac taccacgata cataacgagc cgatctcggg tagaacaacg cccaatgcta ccgctctcag ccaaatcacc ctcagcggag ccaacacgcc taagggacag cctgcggcac catataccag ctcttatgaa gggcatgatc caggagtgag agctttcctc gagcaggttg atttggataa ctaccgagaa cctcttcttg actttggtcc tcgagtggga gcgaaccatc gtaaacgaaa tccgcgcgtc aacaagacgt cgtcttcgtc ttcagcttca gcttcgtcgt cgccaactaa cgtgaccatg attgcccatt tgacacagca tgaaggggcg atcacaggta ttatcacctc gcccgatagc gtctttttcg cttcatcttc agaagatggt caagtattga tctgggatgc agcccgtctg gagagaagtg ttactgcgaa accccgcctg gtatacaaaa tggaagcacc gatagtttcc atgtgtggga tcgataatac atattgcctg gccgtggcga gtgaggatgg tcaggtacat gttttgcgag tgcatacggg cggaggtagt ggagggagta gtacgaggta ttcaaaggca gagtgcatca gaacttggaa caccgaggag tctgatggtc atgtaaggtt tgtctcacat ctgcaagact cgacattgct tttactcaca tcgatgtctg tcattgctac tcttgatatc cgaagcatgg aaatcaaaca acgtttccag catccaccag agctaggcgt catcaccgct tactgcctat cgacccattg gctcatactc ggtacttcca tcggagtgct gagtctttgg gatctgcgtt ttggattact tctcaagtct tggaaagccg gcggaggtgt caccagctgt cgcatccacc cgtcaagggg tcgcggtcga tggatcatgg tttcggtatc gcctacatgg ccatatgctc ttccgggcac ttcgactaac aagtcagagg aatctcagcc ttcgaaacct atcgtggagg tatatgatat cgaaagctct aaattggtgg aagtctacga aataaggagc cgtagatcta gtaaaccttc cgagaccgtt cccgaaccta tagaggtcat tcccaataag gctgctctta tcgcagagct cgctcgctcg acaccctctt tgccttcgct tgtcccaatt gatgactttg acgcgaccaa tcctttaccc acgccgccca gtgccgtact tgatcttatg gtcggacagg gatggactag cttggggagg tcggacgagt ctatattgat gtcggtgccg gagttaggat cttctgacaa gaatggtgag gggcaaggtg gacctggatg gatgattact gctggagagg acaggatagt gagatactgg gatctaatca aagctcaaga tggcttcgtg gtctgcggat ctcaaaaaga gaaggacgtc agtttccgtc gaatgcccga aggttcacca ccaaccatgt tctacactct tccaaatgtc catcgtcaag ggcaaggacc ctctcgggat aaggaacgac aacctctcca accccactat gacgcgatat gtaaacttgg ctcattagaa acgcctttct caagctgcat tataagtgct gatagaagtg gtataataaa agtatggaga atggaaggga tggctggtcc cttgcgttag
Details for CrneC.19306.a.B3.GE40833
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMN DISYDKSLSS SRFLKTILAR HSFGPIVLKI FIKPDATMSL RVIQRRLKLE RDILSELANV NTYQTFVETE KAGYLIRQWI GSNLYDRVSQ QPYLANIEKK WIAFQILTTL RDARNRKVAH GDIKSENILL TSSLSLLLTD FSSSFKPTYL PLDDPSDFSF FFDTSGRRAC YIAPERFYTS DSKVADRKRA SESEGGEWFA RRDGKITEEM DVFSTGCVLA EMWADGRTVF NLSELYAYRD GALNLNGMLE NIHDVDVREM IAQMLSREPS KRPSFDHILS KYRETIFPEY FYTFLQDYIN SLNEKAEDSS FRDDVEGGFL QHSAGQSGAK IDRLLDEWEG IATYLGECPT DNGPAL
Validated NT Sequence
agtagtgccg ggccgttatc ggttgggcat tcaccgagat aagtggcaat cccttcccat tcgtctaata acctgtcaat cttggcacca ctttgtcctg ctgaatgttg caagaatccc ccttccacat catccctaaa actagaatct tccgcctttt cgttgagtga gttaatataa tcttgtaaaa aagtgtaaaa atattcggga aagatggttt cacggtactt tgaaaggata tggtcgaatg agggtcgttt ggaaggttct cttgatagca tttgcgcaat catttctcgg acatcgacgt catggatatt ttccagcatt ccattcaaat ttaaagcgcc atcgcgatac gcatacaact cactgagatt aaataccgtc cgcccatcag cccacatttc tgccaacaca cacccagtac taaagacatc catttcctct gtaatcttcc catccctcct cgcaaaccat tccccacctt cgctctcgga tgcccttttc cgatcagcca ctttggagtc tgaggtgtaa aatcgttccg gtgcgatgta gcatgcgcgt cgcccagagg tatcgaagaa aaatgaaaag tcggaggggt cgtcaagtgg aaggtacgtt ggtttgaagg aggatgaaaa gtccgtcaga agaagagaga gggaggaagt gaggagtatg ttttcagact tgatatcgcc gtgagcaacc ttgcgattgc gagcatctcg aagggtggtt aatatttgga aggcgatcca cttcttttct atattggcaa ggtacggctg ttgactgaca cggtcataca agtttgatcc tatccattgc ctgataagat atccggcctt ctccgtctcc acaaatgtct gatatgtgtt cacattcgca agttctgaaa gaatatccct ctccaacttt aatcttcgct gtataacccg aagtgacatg gttgcatcgg gtttaatgaa tatcttgagg acgatcggcc cgaaactatg gcgagctaag atggtcttga ggaacctgga ggatgacaaa ctcttgtcgt atgagatgtc attcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHND ISYDKSLSSS RFLKTILARH SFGPIVLKIF IKPDATMSLR VIQRRLKLER DILSELANVN TYQTFVETEK AGYLIRQWIG SNLYDRVSQQ PYLANIEKKW IAFQILTTLR DARNRKVAHG DIKSENILLT SSLSLLLTDF SSSFKPTYLP LDDPSDFSFF FDTSGRRACY IAPERFYTSD SKVADRKRAS ESEGGEWFAR RDGKITEEMD VFSTGCVLAE MWADGRTVFN LSELYAYRDG ALNLNGMLEN IHDVDVREMI AQMLSREPSK RPSFDHILSK YRETIFPEYF YTFLQDYINS LNEKAEDSSF RDDVEGGFLQ HSAGQSGAKI DRLLDEWEGI ATYLGECPTD NGPALLILNV ITS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatgaca tctcatacga caagagtttg tcatcctcca ggttcctcaa gaccatctta gctcgccata gtttcgggcc gatcgtcctc aagatattca ttaaacccga tgcaaccatg tcacttcggg ttatacagcg aagattaaag ttggagaggg atattctttc agaacttgcg aatgtgaaca catatcagac atttgtggag acggagaagg ccggatatct tatcaggcaa tggataggat caaacttgta tgaccgtgtc agtcaacagc cgtaccttgc caatatagaa aagaagtgga tcgccttcca aatattaacc acccttcgag atgctcgcaa tcgcaaggtt gctcacggcg atatcaagtc tgaaaacata ctcctcactt cctccctctc tcttcttctg acggactttt catcctcctt caaaccaacg taccttccac ttgacgaccc ctccgacttt tcatttttct tcgatacctc tgggcgacgc gcatgctaca tcgcaccgga acgattttac acctcagact ccaaagtggc tgatcggaaa agggcatccg agagcgaagg tggggaatgg tttgcgagga gggatgggaa gattacagag gaaatggatg tctttagtac tgggtgtgtg ttggcagaaa tgtgggctga tgggcggacg gtatttaatc tcagtgagtt gtatgcgtat cgcgatggcg ctttaaattt gaatggaatg ctggaaaata tccatgacgt cgatgtccga gaaatgattg cgcaaatgct atcaagagaa ccttccaaac gaccctcatt cgaccatatc ctttcaaagt accgtgaaac catctttccc gaatattttt acactttttt acaagattat attaactcac tcaacgaaaa ggcggaagat tctagtttta gggatgatgt ggaaggggga ttcttgcaac attcagcagg acaaagtggt gccaagattg acaggttatt agacgaatgg gaagggattg ccacttatct cggtgaatgc ccaaccgata acggcccggc actactcatc ttgaacgtga tcacctcttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat