CrneC.19305.a

Uncharacterized protein

CENTER ID: CrneC.19305.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19305.a.B2.GE40832 P-loop NTPase 6 258
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_00266
UniProt: J9VIK3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQVTPEQLVN EIVKKYNELP KDRRLLVAIA GPPGSGKSTL AYPLADALNS FILGHPPTNP SHIETPVSSL LAEGSSQQGN GEEVALTIGL DGWHYRREEL DGFDDPQDAH WRRGASFTFD LNSYKAFLLL LRLPLCPHPP KNIPFPTFDH ASKDPKPSPF PILPGHRIIL IEGLYTLLDQ PGWRDCAAMM DIGVWVDVDE KVARKRVIKR NWEAGIVEDV KKCEERVDAV DMKNGEQVRN YLASPTYIIR SIDGELLSKE SFRFD
NT Sequence
atgcaggtca cccctgaaca actcgtcaac gaaattgtta agaaatataa cgaacttcca aaagatcgga gactcctagt cgcaatagca gggccacctg gatcaggtaa atctactctc gcatatcccc tcgccgacgc cctcaactcc ttcatccttg gccatccacc cacaaatcct tctcatattg agactcctgt ttcatctttg cttgcagagg ggagcagcca gcaagggaat ggcgaagagg ttgccttaac gataggttta gatggatggc attatcgtcg agaggaatta gacggctttg atgatcctca ggacgctcat tggagaaggg gggcctcctt tacctttgat ttgaattcat ataaagcttt tctcttactt ctccgccttc ctctttgccc tcacccgccg aaaaatattc cttttccaac attcgatcat gcttctaaag atcccaagcc ctcacctttc cctatcttac ctggacaccg catcatcctc atagaaggtc tctatactct tcttgaccaa cctggctgga gagattgtgc ggcaatgatg gatataggcg tctgggttga tgtggatgag aaggtagcaa gaaaacgagt aataaaaaga aattgggagg ccggaatcgt tgaggatgtc aaaaaatgtg aagaaagagt cgatgccgtg gacatgaaaa atggcgaaca ggtcaggaac tatcttgcca gtccgacata tatcattaga tctatcgacg gggagctcct ctcgaaggaa agtttccgtt tcgactaa
Details for CrneC.19305.a.B2.GE40832
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME QLVNEIVKKY NELPKDRRLL VAIAGPPGSG KSTLAYPLAD ALNSFILGHP PTNPSHIETP VSSLLAEGSS QQGNGEEVAL TIGLDGWHYR REELDGFDDP QDAHWRRGAS FTFDLNSYKA FLLLLRLPLC PHPPKNIPFP TFDHASKDPK PSPFPILPGH RIILIEGLYT LLDQPGWRDC AAMMDIGVWV DVDEKVARKR VIKRNWEAGI VEDVKKCEER VDAVDMKNGE QVRNYLASPT YIIRSIDGEL LS
Validated NT Sequence
atcttactca cttacgagag gagctccccg tcgatagatc taatgatata tgtcggactg gcaagatagt tcctgacctg ttcgccattt ttcatgtcca cggcatcgac tctttcttca cattttttga catcctcaac gattccggcc tcccaatttc tttttattac tcgttttctt gctaccttct catccacatc aacccagacg cctatatcca tcattgccgc acaatctctc cagccaggtt ggtcaagaag agtatagaga ccttctatga ggatgatgcg gtgtccaggt aagataggga aaggtgaggg cttgggatct ttagaagcat gatcgaatgt tggaaaagga atatttttcg gcgggtgagg gcaaagagga aggcggagaa gtaagagaaa agctttatat gaattcaaat caaaggtaaa ggaggccccc cttctccaat gagcgtcctg aggatcatca aagccgtcta attcctctcg acgataatgc catccatcta aacctatcgt taaggcaacc tcttcgccat tcccttgctg gctgctcccc tctgcaagca aagatgaaac aggagtctca atatgagaag gatttgtggg tggatggcca aggatgaagg agttgagggc gtcggcgagg ggatatgcga gagtagattt acctgatcca ggtggccccg ctattgcgac taggagtctc cgatcttttg gaagttcgtt atatttctta acaatttcgt tgacgagttg ttccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHEQ LVNEIVKKYN ELPKDRRLLV AIAGPPGSGK STLAYPLADA LNSFILGHPP TNPSHIETPV SSLLAEGSSQ QGNGEEVALT IGLDGWHYRR EELDGFDDPQ DAHWRRGASF TFDLNSYKAF LLLLRLPLCP HPPKNIPFPT FDHASKDPKP SPFPILPGHR IILIEGLYTL LDQPGWRDCA AMMDIGVWVD VDEKVARKRV IKRNWEAGIV EDVKKCEERV DAVDMKNGEQ VRNYLASPTY IIRSIDGELL S
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaacaac tcgtcaacga aattgttaag aaatataacg aacttccaaa agatcggaga ctcctagtcg caatagcagg gccacctgga tcaggtaaat ctactctcgc atatcccctc gccgacgccc tcaactcctt catccttggc catccaccca caaatccttc tcatattgag actcctgttt catctttgct tgcagagggg agcagccagc aagggaatgg cgaagaggtt gccttaacga taggtttaga tggatggcat tatcgtcgag aggaattaga cggctttgat gatcctcagg acgctcattg gagaaggggg gcctccttta cctttgattt gaattcatat aaagcttttc tcttacttct ccgccttcct ctttgccctc acccgccgaa aaatattcct tttccaacat tcgatcatgc ttctaaagat cccaagccct cacctttccc tatcttacct ggacaccgca tcatcctcat agaaggtctc tatactcttc ttgaccaacc tggctggaga gattgtgcgg caatgatgga tataggcgtc tgggttgatg tggatgagaa ggtagcaaga aaacgagtaa taaaaagaaa ttgggaggcc ggaatcgttg aggatgtcaa aaaatgtgaa gaaagagtcg atgccgtgga catgaaaaat ggcgaacagg tcaggaacta tcttgccagt ccgacatata tcattagatc tatcgacggg gagctcctct cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat