CrneC.19164.a

Pantothenate kinase

CENTER ID: CrneC.19164.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.19164.a.B2.GE40831 kinase domain 131 510
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_02866
UniProt: J9VQ48

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPAQSSMPDI SIPQARRIVV DTTGAQIVQE ESPATRDSRG IYLPHYTEPV SHIAIDVGGS LAKVVYFTRS NLPIPATPSS SGTSSPFLPP SQSVSQPTAI VSEGSPMPSY FPRDSPTLNP HSHAPFDPSP AEQRRPTVNG ALTPATLSED NSAIPLPAKN KHKSRNSIHN PLPPPLPGGL LNFARFETDN IDELIIFLQD LISSSATANR VSLEKMKRNV KVMATGGGAH KYYDRLADEL GVEVHREEEM ECLIRGLGFV TRVPEEVFYF SEELVYKVSH AGSTSEQTLT IPASELPRPS PMPPAYQVTF APTPSDDDPV PHFPCLVVNI GSGVSIVKVD EDGSFERVSG TSLGGGTLWG LLSLLTDAES FDEMLLLSEK GDNSAVDMLV GDIYGSDYNK IGLKSSTIAS SFGKVFKKGS NAERRKRFKQ EDIAKSLLYA ISNNIGHVAY MNAAKYGLDK VFFGGCFIRG HAATISTLSY AIRFWSKGTM TACFLRHEGF LGAIGAWIKN VGELDENGSP LQKHSD
NT Sequence
atgcctgcac aatcatctat gcccgatatc tcaattcctc aggccaggag aatagtggta gataccactg gggcgcaaat agttcaggag gagtcccctg caactcgaga ctcccgcggt atatatttac cccattatac tgagccagtt tcacacattg ccatagacgt cggaggttcg ttggcaaagg ttgtatactt tacccgttca aatctcccta tacctgcaac gcccagttca tccggaacat catctccttt ccttcctcct tcccaatctg tgagccaacc gacggctatt gtctccgagg gatctcccat gccctcttat tttccccgag attcaccaac gctcaaccct cactctcatg cgccttttga cccttcacca gcagaacaac gtcgcccaac agtaaatggc gccctcactc cagccaccct gtcagaagac aattctgcta tacccttacc ggcaaagaac aaacacaaat cacgtaactc cattcataac cctttacccc ctcctttacc tgggggtctt ttgaactttg ctcgctttga aaccgacaat atagatgagc ttattatttt ccttcaagat ctgatatctt catcagcaac agccaacaga gtctcgttgg agaagatgaa gcgtaacgtc aaggtcatgg caactggtgg tggagcacac aaatattacg acaggctggc tgatgagttg ggggtggaag tgcatcgcga ggaagagatg gaatgcctaa tcagaggcct gggattcgtg actcgagtac ccgaagaggt tttctacttc tcagaagaac tggtctataa ggtttcacac gcaggctcca cttctgagca gacgctcact atcccagcat ccgaactgcc cagaccttca ccaatgccgc cggcatatca ggttactttt gccccgacac cgtcagacga cgatcctgtg cctcatttcc catgcctggt ggtgaatatt ggcagtggtg tcagcattgt aaaagtggac gaagatggta gctttgagcg tgtgagcgga acatctcttg ggggaggaac attgtggggc ttgctgagtc tcttgactga tgccgaatca tttgatgaga tgctgctgtt atcggaaaaa ggagacaatt ctgccgtcga tatgcttgtt ggcgacattt atggttcgga ctacaacaag ataggcctta agtcatcgac cattgcatcg tcgtttggaa aagtatttaa aaaaggatcg aatgcggaga ggagaaagag attcaagcaa gaagatatcg caaagagcct cctttatgcg atcagtaata acattgggca tgtcgcgtac atgaacgcgg ccaaatacgg tctagataag gtctttttcg gaggttgttt tattcgagga catgctgcta ccatttctac tctctcttat gctattcgct tctggagcaa aggtactatg acggcgtgct ttctcagaca cgagggattc ttgggagcga ttggggcttg gatcaagaat gtgggagaac tagatgagaa tggaagccct ctacaaaagc attcagatta g
Details for CrneC.19164.a.B2.GE40831
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA EQRRPTVNGA LTPATLSEDN SAIPLPAKNK HKSRNSIHNP LPPPLPGGLL NFARFETDNI DELIIFLQDL ISSSATANRV SLEKMKRNVK VMATGGGAHK YYDRLADELG VEVHREEEME CLIRGLGFVT RVPEEVFYFS EELVYKVSHA GSTSEQTLTI PASELPRPSP MPPAYQVTFA PTPSDDDPVP HFPCLVVNIG SGVSIVKVDE DGSFERVSGT SLGGGTLWGL LSLLTDAESF DEMLLLSEKG DNSAVDMLVG DIYGSDYNKI GLKSSTIASS FGKVFKKGSN AERRKRFKQE DIAKSLLYAI SNNIGHVAYM NAAKYGLDKV FFGGCFIRGH AATISTLSYA IRFWSKGTMT ACFLRHEGFL GA
Validated NT Sequence
tcgctcccaa gaatccctcg tgtctgagaa agcacgccgt catagtacct ttgctccaga agcgaatagc ataagagaga gtagaaatgg tagcagcatg tcctcgaata aaacaacctc cgaaaaagac cttatctaga ccgtatttgg ccgcgttcat gtacgcgaca tgcccaatgt tattactgat cgcataaagg aggctctttg cgatatcttc ttgcttgaat ctctttctcc tctccgcatt cgatcctttt ttaaatactt ttccaaacga cgatgcaatg gtcgatgact taaggcctat cttgttgtag tccgaaccat aaatgtcgcc aacaagcata tcgacggcag aattgtctcc tttttccgat aacagcagca tctcatcaaa tgattcggca tcagtcaaga gactcagcaa gccccacaat gttcctcccc caagagatgt tccgctcaca cgctcaaagc taccatcttc gtccactttt acaatgctga caccactgcc aatattcacc accaggcatg ggaaatgagg cacaggatcg tcgtctgacg gtgtcggggc aaaagtaacc tgatatgccg gcggcattgg tgaaggtctg ggcagttcgg atgctgggat agtgagcgtc tgctcagaag tggagcctgc gtgtgaaacc ttatagacca gttcttctga gaagtagaaa acctcttcgg gtactcgagt cacgaatccc aggcctctga ttaggcattc catctcttcc tcgcgatgca cttccacccc caactcatca gccagcctgt cgtaatattt gtgtgctcca ccaccagttg ccatgacctt gacgttacgc ttcatcttct ccaacgagac tctgttggct gttgctgatg aagatatcag atcttgaagg aaaataataa gctcatctat attgtcggtt tcaaagcgag caaagttcaa aagaccccca ggtaaaggag ggggtaaagg gttatgaatg gagttacgtg atttgtgttt gttctttgcc ggtaagggta tagcagaatt gtcttctgac agggtggctg gagtgagggc gccatttact gttgggcgac gttgttctgc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHAE QRRPTVNGAL TPATLSEDNS AIPLPAKNKH KSRNSIHNPL PPPLPGGLLN FARFETDNID ELIIFLQDLI SSSATANRVS LEKMKRNVKV MATGGGAHKY YDRLADELGV EVHREEEMEC LIRGLGFVTR VPEEVFYFSE ELVYKVSHAG STSEQTLTIP ASELPRPSPM PPAYQVTFAP TPSDDDPVPH FPCLVVNIGS GVSIVKVDED GSFERVSGTS LGGGTLWGLL SLLTDAESFD EMLLLSEKGD NSAVDMLVGD IYGSDYNKIG LKSSTIASSF GKVFKKGSNA ERRKRFKQED IAKSLLYAIS NNIGHVAYMN AAKYGLDKVF FGGCFIRGHA ATISTLSYAI RFWSKGTMTA CFLRHEGFLG AIGAWIKN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcagaac aacgtcgccc aacagtaaat ggcgccctca ctccagccac cctgtcagaa gacaattctg ctataccctt accggcaaag aacaaacaca aatcacgtaa ctccattcat aaccctttac cccctccttt acctgggggt cttttgaact ttgctcgctt tgaaaccgac aatatagatg agcttattat tttccttcaa gatctgatat cttcatcagc aacagccaac agagtctcgt tggagaagat gaagcgtaac gtcaaggtca tggcaactgg tggtggagca cacaaatatt acgacaggct ggctgatgag ttgggggtgg aagtgcatcg cgaggaagag atggaatgcc taatcagagg cctgggattc gtgactcgag tacccgaaga ggttttctac ttctcagaag aactggtcta taaggtttca cacgcaggct ccacttctga gcagacgctc actatcccag catccgaact gcccagacct tcaccaatgc cgccggcata tcaggttact tttgccccga caccgtcaga cgacgatcct gtgcctcatt tcccatgcct ggtggtgaat attggcagtg gtgtcagcat tgtaaaagtg gacgaagatg gtagctttga gcgtgtgagc ggaacatctc ttgggggagg aacattgtgg ggcttgctga gtctcttgac tgatgccgaa tcatttgatg agatgctgct gttatcggaa aaaggagaca attctgccgt cgatatgctt gttggcgaca tttatggttc ggactacaac aagataggcc ttaagtcatc gaccattgca tcgtcgtttg gaaaagtatt taaaaaagga tcgaatgcgg agaggagaaa gagattcaag caagaagata tcgcaaagag cctcctttat gcgatcagta ataacattgg gcatgtcgcg tacatgaacg cggccaaata cggtctagat aaggtctttt tcggaggttg ttttattcga ggacatgctg ctaccatttc tactctctct tatgctattc gcttctggag caaaggtact atgacggcgt gctttctcag acacgaggga ttcttgggag cgattggggc ttggatcaag aattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat