CrneC.18709.d

Aur protein kinase

CENTER ID: CrneC.18709.d
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.18709.d.B2.GE40830 kinase 203 460
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_01285
UniProt: J9VK66

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSQNVSGLM AGLSLYAPQS PRGSKSQQAG KTQESSHSSQ SQNRLPPVLK KYMNPGLVRP PNSGLANNSH PSSSCSDTSR GPLLTLAGVN VSYPKSGALS PTSKFKGASG YHSAHGIHGP AHSTSSRAMA SSINPGASKH SAMTSSAHIT NSASENCKGL ELGRYDGGLE EDEANHGDVS GPTAKMLELS SVNDGAIPLS LPSFTIGRPL GKGKFGRVYL ARSKAPPHFI VALKCLHKAE IIQGKVESQV RREIEIQQNL RHPNILRLYG YFHDSKRIFL VLEFAAKGEL YKQLSRLGRF DEKKSSRYIA QMADALSYLH KKHVIHRDIK PENLLIGLNG ELKIGDFGWS VHAPSNRRST LCGTLDYLPP EMVEGKEHTA AVDLWALGVL TYEFVVGGPP FEDLSGNAAT YRRIRNVDLH VPSWVSPEAT DLIKRLLRYK PEDRLPLSQV MVHPWIKMYE KKRSTSSGIR KS
NT Sequence
atgtcctcgc agaatgtttc tggcctcatg gcaggtctat cactctatgc tccgcaatct cccagagggt ccaagtcaca acaagcaggc aagacacagg agtcttctca ctcttcccag tctcagaata ggctccctcc ggtgctcaag aagtatatga atccaggcct tgtccgcccc cccaacagtg gtcttgccaa caacagtcat ccgtcttcct cttgctcaga cacatcacgc ggtccgcttc ttacccttgc gggtgtgaat gtctcatatc ccaaatccgg cgcattgtct cctacgagca agtttaaggg cgctagcgga taccattctg ctcatggaat ccacggacca gcccattcaa cgtcctcaag agcgatggcg tcttccatca atcctggtgc ttcgaaacat tctgcaatga ccagttctgc acatattact aattcggctt cggaaaactg caagggtcta gaattggggc ggtatgatgg cggtttggag gaagatgaag caaatcatgg ggatgtcagt gggcctacag ccaaaatgct tgagctgtct agtgtcaacg acggcgcgat tccattatcc ctaccgtcgt tcactattgg ccgcccgtta ggtaaaggga aattcggtcg cgtatacctt gctagatcta aggcacctcc tcactttatc gtcgccctca aatgtctcca taaggcagaa atcattcagg gtaaggtaga aagccaggtg cgaagagaga tagaaattca gcaaaacttg aggcatccta atattcttcg tctctatgga tacttccatg acagcaaacg tatatttttg gtcctggaat tcgccgcaaa gggggaattg tacaaacaac tgtccagatt aggcagattt gacgagaaaa agagtagccg atatatagct cagatggccg atgctttgtc ctacttgcac aaaaagcacg tcattcaccg agacatcaaa cctgaaaatc ttctcatcgg tttgaatggc gagctcaaaa tcggcgattt tggctggagc gtgcatgcac caagtaatcg ccgcagcacg ctttgtggaa cccttgatta tcttccgcct gagatggtgg aaggcaagga gcatactgca gctgtcgatt tatgggcatt aggtgtgcta acttacgagt ttgtcgtcgg tggaccccca ttcgaggatc tttctggaaa cgccgctacc taccggcgaa taagaaatgt tgatttacac gttccctcct gggtttctcc tgaagccact gatctcatca aaagactcct tcggtacaaa cctgaggatc gtttacctct gtcccaggtc atggttcacc cgtggatcaa gatgtacgag aagaaaaggt cgacaagtag cggtatcagg aagtcataa
Details for CrneC.18709.d.B2.GE40830
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS FTIGRPLGKG KFGRVYLARS KAPPHFIVAL KCLHKAEIIQ GKVESQVRRE IEIQQNLRHP NILRLYGYFH DSKRIFLVLE FAAKGELYKQ LSRLGRFDEK KSSRYIAQMA DALSYLHKKH VIHRDIKPEN LLIGLNGELK IGDFGWSVHA PSNRRSTLCG TLDYLPPEMV EGKEHTAAVD LWALGVLTYE FVVGGPPFED LSGNAATYRR IRNVDLHVPS WVSPEATDLI KRLLRYKPED RLPLSQVMVH PWIKMYE
Validated NT Sequence
tcacttactc gtacatcttg atccacgggt gaaccatgac ctgggacaga ggtaaacgat cctcaggttt gtaccgaagg agtcttttga tgagatcagt ggcttcagga gaaacccagg agggaacgtg taaatcaaca tttcttattc gccggtaggt agcggcgttt ccagaaagat cctcgaatgg gggtccaccg acgacaaact cgtaagttag cacacctaat gcccataaat cgacagctgc agtatgctcc ttgccttcca ccatctcagg cggaagataa tcaagggttc cacaaagcgt gctgcggcga ttacttggtg catgcacgct ccagccaaaa tcgccgattt tgagctcgcc attcaaaccg atgagaagat tttcaggttt gatgtctcgg tgaatgacgt gctttttgtg caagtaggac aaagcatcgg ccatctgagc tatatatcgg ctactctttt tctcgtcaaa tctgcctaat ctggacagtt gtttgtacaa ttcccccttt gcggcgaatt ccaggaccaa aaatatacgt ttgctgtcat ggaagtatcc atagagacga agaatattag gatgcctcaa gttttgctga atttctatct ctcttcgcac ctggctttct accttaccct gaatgatttc tgccttatgg agacatttga gggcgacgat aaagtgagga ggtgccttag atctagcaag gtatacgcga ccgaatttcc ctttacctaa cgggcggcca atagtgaacg acatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHSF TIGRPLGKGK FGRVYLARSK APPHFIVALK CLHKAEIIQG KVESQVRREI EIQQNLRHPN ILRLYGYFHD SKRIFLVLEF AAKGELYKQL SRLGRFDEKK SSRYIAQMAD ALSYLHKKHV IHRDIKPENL LIGLNGELKI GDFGWSVHAP SNRRSTLCGT LDYLPPEMVE GKEHTAAVDL WALGVLTYEF VVGGPPFEDL SGNAATYRRI RNVDLHVPSW VSPEATDLIK RLLRYKPEDR LPLSQVMVHP WIKMYE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcgttca ctattggccg cccgttaggt aaagggaaat tcggtcgcgt ataccttgct agatctaagg cacctcctca ctttatcgtc gccctcaaat gtctccataa ggcagaaatc attcagggta aggtagaaag ccaggtgcga agagagatag aaattcagca aaacttgagg catcctaata ttcttcgtct ctatggatac ttccatgaca gcaaacgtat atttttggtc ctggaattcg ccgcaaaggg ggaattgtac aaacaactgt ccagattagg cagatttgac gagaaaaaga gtagccgata tatagctcag atggccgatg ctttgtccta cttgcacaaa aagcacgtca ttcaccgaga catcaaacct gaaaatcttc tcatcggttt gaatggcgag ctcaaaatcg gcgattttgg ctggagcgtg catgcaccaa gtaatcgccg cagcacgctt tgtggaaccc ttgattatct tccgcctgag atggtggaag gcaaggagca tactgcagct gtcgatttat gggcattagg tgtgctaact tacgagtttg tcgtcggtgg acccccattc gaggatcttt ctggaaacgc cgctacctac cggcgaataa gaaatgttga tttacacgtt ccctcctggg tttctcctga agccactgat ctcatcaaaa gactccttcg gtacaaacct gaggatcgtt tacctctgtc ccaggtcatg gttcacccgt ggatcaagat gtacgagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at