CrneC.18709.c

STE/STE7/MKK protein kinase

CENTER ID: CrneC.18709.c
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.18709.c.B2.GE40829 kinase 333 621
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_02357
UniProt: J9VLX8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDNTLAPSKG KKPGGARPNP SSRPEGGGPT RPAGASGVPK LSIPPGNVPG MHVDQAGQGG WHQPASLPSL ALRPMRPSPS PSSSSRPKLS LAPLTPTIPA SSSSAPSSAA LAPPNRPALL NAQSARGYGE RSTPSLKLSI PGASSVGPGF STEHDYPLEL PDDTDALSSE LKTPTPGHPA GGGLNLSLSS LSLTSSEDAN PTLQARGRDY DEGESSYGYG CVGNGLIGPG GDAISAMTED IRQALSRNRF ETESDRGGAR SRAGSLMSDS SRTTAGRDRS DSASLYNSAS ARESPSASLC GGSARQSLDL PRAAEEVSEQ TGSPVFDPEG LVMMKRLGEG TGGSVDMVQD RATGRIMAKK VITRTSNPMV HKQLLRELEI LNSCASPFIV EHYGSFLADH DSSIGILMEY CEAGSLDSLL GKMKKKNMRC SEHVLGRVAS SVLKGLDYLH QRRIVHRDIK PSNILITRQG AVKLCDFGVS GELVESLAGT FTGTSFYMAP ERIQNKPYSI KADVWSLGMT LHEIAHLRFP FPPEGENQSV APIELLSYIV TAPVPVMIDD LSVGRMWSEP IKDFMGQCLI RSGTNRPYPW QLLQHPFIVA NEAKKVNMAK WVAALCDWPL S
NT Sequence
atggataata cccttgcgcc atctaaaggc aaaaagcccg gaggcgccag acctaaccct agcagtcgac ctgaaggcgg tgggccgacc aggccagccg gggcgagtgg tgtgccaaag ctcagtatac ctccaggcaa cgtcccaggt atgcatgttg accaagcagg ccagggagga tggcatcagc ccgcctctct cccatctctt gcgctccgtc caatgcgccc ttccccatcc ccctcatctt catcccgccc taaactatct ctggccccgc ttaccccgac tattcctgcg tcgtcaagct ctgccccttc ttcagcggcc ctagcacctc ccaatagacc agcacttttg aacgctcagt ctgctagagg atatggggaa agaagcacac caagcttgaa actttctatt ccaggggcca gctccgttgg accaggcttt tctacagaac acgattatcc tcttgaactg ccggatgata ccgacgcatt gtcctctgaa ctgaagacac cgacgcctgg acatcctgct ggaggaggct tgaatctaag tctgtcttcc ctctcactta cgtcttctga ggatgcgaat cccacattac aagctcgcgg gagggactat gatgaaggag aatcgtcata tggttacggg tgtgtgggta acggtctgat tggacctggg ggagatgcga tcagcgccat gacggaggat attagacaag cactgtctcg aaaccggttt gaaactgagt ccgacagggg cggggcgagg agccgagcgg gcagtttgat gagcgatagt agccggacta cagcagggcg tgatagatca gattcggcat cattatataa ctcagcttca gcgcgtgaat cgccgtctgc tagtctatgc ggaggatcag ctaggcaaag tctggatcta ccgcgagcag cagaagaggt gagcgagcag acgggatcac cagtatttga tcctgaagga ctagtgatga tgaagaggct cggtgaaggg acaggtggct cggtggatat ggtacaggac cgagccacag gtcgcattat ggccaagaag gttatcactc ggacatcgaa cccaatggtg cacaagcagc tccttcgtga actagagatt cttaattcct gcgcttcgcc gttcattgtg gaacattatg gatctttctt ggccgaccat gattcgtcaa taggaattct catggagtat tgcgaagccg gcagtttaga tagtcttctg ggtaagatga agaagaagaa tatgaggtgt tccgaacatg tcctaggtag agttgcttct tcggtgttga aaggtcttga ttatctccac caacggcgga tcgttcatcg agacatcaaa ccctccaata tccttatcac tcgacaggga gccgtcaaac tttgcgactt tggagtcagt ggagagctgg tagagtcttt ggcggggact tttactggga caagctttta catggcccct gaaagaatac aaaacaagcc ttattcaatc aaggccgatg tctggtctct cggcatgacc ttgcacgaga tcgcccatct ccgatttcca tttcctcccg agggcgaaaa tcaatcagtt gcacctatag agttattatc atacattgtg acggcacccg taccggtaat gatcgatgat ttaagtgtgg ggagaatgtg gagcgagcct atcaaagatt tcatgggaca atgcttgata agatcaggaa caaatagacc ctacccttgg caactactgc agcatccctt cattgtcgcc aatgaagcta agaaggttaa catggccaaa tgggttgcag cactttgcga ctggcctcta tcatag
Details for CrneC.18709.c.B2.GE40829
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMM KRLGEGTGGS VDMVQDRATG RIMAKKVITR TSNPMVHKQL LRELEILNSC ASPFTVEHYG SFLADHDSSI GILMEYCEAG SLDSLLGKMK KKNMRCSEHV LGRVASSVLK GLDYLHQRRI VHRDIKPSNI LITRQGAVKL CDFGVSGELV ESLAGTFTGT SFYMAPERIQ NKPYSIKADV WSLGMTLHEI AHLRFPFPPE GENQSVAPIE LLSYIVTAPV PVMIDDLSVG RMWSEPIKDF MGQCLIRSGT NRPYPWQLLQ HPFIVANEAK KVNMAKWVAA LCDWPLS
Validated NT Sequence
atcttactca cttatgatag aggccagtcg caaagtgctg caacccattt ggccatgtta accttcttag cttcattggc gacaatgaag ggatgctgca gtagttgcca agggtagggt ctatttgttc ctgatcttat caagcattgt cccatgaaat ctttgatagg ctcgctccac attctcccca cacttaaatc atcgatcatt accggtacgg gtgccgtcac aatgtatgat aataactcta taggtgcaac tgattgattt tcgccctcgg gaggaaatgg aaatcggaga tgggcgatct cgtgcaaggt catgccgaga gaccagacat cggccttgat tgaataaggc ttgttttgta ttctttcagg ggccatgtaa aagcttgtcc cagtaaaagt ccccgccaaa gactctacca gctctccact gactccaaag tcgcaaagtt tgacggctcc ctgtcgagtg ataaggatat tggagggttt gatgtctcga tgaacgatcc gccgttggtg gagataatca agacctttca acaccgaaga agcaactcta cctaggacat gttcggaaca cctcatattc ttcttcttca tcttacccag aagactatct aaactgccgg cttcgcaata ctccatgaga attcctattg acgaatcatg gtcggccaag aaagatccat aatgttccac agtgaacggc gaagcgcagg aattaagaat ctctagttca cgaaggagct gcttgtgcac cattgggttc gatgtccgag tgataacctt cttggccata atgcgacctg tggctcggtc ctgtaccata tccaccgagc cacctgtccc ttcaccgagc ctcttcatca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMM KRLGEGTGGS VDMVQDRATG RIMAKKVITR TSNPMVHKQL LRELEILNSC ASPFIVEHYG SFLADHDSSI GILMEYCEAG SLDSLLGKMK KKNMRCSEHV LGRVASSVLK GLDYLHQRRI VHRDIKPSNI LITRQGAVKL CDFGVSGELV ESLAGTFTGT SFYMAPERIQ NKPYSIKADV WSLGMTLHEI AHLRFPFPPE GENQSVAPIE LLSYIVTAPV PVMIDDLSVG RMWSEPIKDF MGQCLIRSGT NRPYPWQLLQ HPFIVANEAK KVNMAKWVAA LCDWPLS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatga agaggctcgg tgaagggaca ggtggctcgg tggatatggt acaggaccga gccacaggtc gcattatggc caagaaggtt atcactcgga catcgaaccc aatggtgcac aagcagctcc ttcgtgaact agagattctt aattcctgcg cttcgccgtt cattgtggaa cattatggat ctttcttggc cgaccatgat tcgtcaatag gaattctcat ggagtattgc gaagccggca gtttagatag tcttctgggt aagatgaaga agaagaatat gaggtgttcc gaacatgtcc taggtagagt tgcttcttcg gtgttgaaag gtcttgatta tctccaccaa cggcggatcg ttcatcgaga catcaaaccc tccaatatcc ttatcactcg acagggagcc gtcaaacttt gcgactttgg agtcagtgga gagctggtag agtctttggc ggggactttt actgggacaa gcttttacat ggcccctgaa agaatacaaa acaagcctta ttcaatcaag gccgatgtct ggtctctcgg catgaccttg cacgagatcg cccatctccg atttccattt cctcccgagg gcgaaaatca atcagttgca cctatagagt tattatcata cattgtgacg gcacccgtac cggtaatgat cgatgattta agtgtgggga gaatgtggag cgagcctatc aaagatttca tgggacaatg cttgataaga tcaggaacaa atagacccta cccttggcaa ctactgcagc atcccttcat tgtcgccaat gaagctaaga aggttaacat ggccaaatgg gttgcagcac tttgcgactg gcctctatca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat