CrneC.18709.a

AGC/Akt protein kinase

CENTER ID: CrneC.18709.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.18709.a.B4.GE41411 kinase construct 3 237 564
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_04678
UniProt: J9W0G9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMSWKFGKKF KEGGFLSGKH HSSNNNSPSD TSRSTTPTPG NPHPEDAVKP PVPRSGMLKI RVTAAKGLSL PQGVSVPAPV QEALTTHPTL ASRIATSPPT AIVKAAGANR DSLQRRQVWW LPYLVLEFDK NEVLVDALGG DLASPVWMYS ATFDVSRISE ISATVYLRTR EPHAEGREKS NGEGEGEDMG NSDLCLGSIR FTPNLDSLRV TDDWVTVQGG GGSGSINVQV SFKPASGQIL TIDSFELLKV IGKGSFGKVM QVRKRDTLRI YALKTIRKAH IVSRSEVTHT LAERTVLAQV NCPFIVPLKF SFQSKEKLYL VLAFINGGEL FHHLQREGKF NETRSRFYSA QLLLALEHLH SFNVIYRDLK PENILLDYAG NIALCDFGLC KLNMSNSDTT NTFCGTPEYL APELLSGHGY TKCVDWWTLG VLLYEMLTGL PPFYDENTNE MYRKILTEPL RFPDGVRSEA RSLLTGLLNR DPRQRLGVNG AQDIKNHPFF AKHINFTKLW NKQIQPPFKP AVASAIDTSN FDEEFTNEVP LDSVVDDSHL SQTVQQQFEG FSWSVSPLGE SVGRY
NT Sequence
atgatgtcct ggaaatttgg taaaaaattc aaagaaggag ggttcctatc gggcaaacac cacagctcca acaacaacag cccttccgat acctctcgtt ccaccactcc tacccctggc aacccccacc ctgaagatgc agtcaagccg cctgtgccta ggagtggtat gctcaagatc cgtgtgactg ccgcaaaggg acttagtttg ccgcaaggag tctctgtccc tgccccagtc caggaagctc ttaccaccca tcctaccctt gcttcccgca tagctacatc tcctcccacc gccatcgtca aggcggctgg tgccaatcgt gactctctcc agcgtcgcca agtctggtgg cttccctatc tcgttcttga gttcgacaaa aatgaagtcc tcgtcgatgc gcttggcggt gacttggcat cgccagtatg gatgtacagt gcgacttttg atgtttctag gataagcgag attagcgcta ctgtgtactt gaggacaagg gagcctcatg cagagggccg agaaaagtct aacggtgaag gcgagggcga ggatatgggt aactcagact tgtgcttggg tagcatcaga ttcacgccca acttggactc tttgagggtg acagacgact gggtgactgt gcaaggcggt ggtggctcag ggtctattaa tgttcaagtg tccttcaagc ctgcttctgg gcaaattctt acgattgata gctttgagct tcttaaagtt attggcaagg gcagtttcgg taaagtcatg caagtccgca agcgtgacac gctccgaatt tatgctctca aaaccatccg aaaagcccac attgtctccc gatccgaagt tacccacacc ttggcagaac gtaccgtcct cgcccaggtt aactgccctt ttattgtgcc cttaaagttc tctttccagt ccaaggaaaa gctgtacctg gtcttggcgt tcatcaacgg aggagagttg ttccaccact tgcagaggga gggcaagttc aacgagacta ggtctaggtt ctactcggcc caactactgc tggcgttgga gcatttgcac tccttcaatg tgatctacag agatttgaag cccgaaaaca ttctgttaga ttacgccggg aatattgccc tttgtgattt tggactgtgt aaactcaaca tgtccaactc ggacaccaca aataccttct gtggtacacc tgaatatctt gctcctgaac ttctttctgg tcacggctat actaaatgcg ttgactggtg gactcttggt gtcctcttgt acgaaatgct cactggtctt cctccctttt acgacgaaaa caccaacgaa atgtaccgca agattctcac cgaacctttg cgcttccccg atggcgttcg ttctgaagcc aggtctcttc tgaccggttt gcttaatcga gatcctcgcc aaaggcttgg cgtcaacggc gcccaggata tcaagaatca ccccttcttt gccaaacaca tcaactttac aaaactctgg aacaagcaaa tccaaccacc tttcaagccc gctgtggcga gtgctattga cacttccaat ttcgatgagg agttcaccaa cgaggtgccc ctcgactcgg ttgttgacga ctcacatctg tcccagactg tgcaacagca attcgaggga ttctcctgga gtgtttcgcc tcttggcgaa tctgttggcc gatattag
Details for CrneC.18709.a.B4.GE41411
HARVESTED ON: 4/5/2017
SEQUENCED ON: 4/27/2017
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG QILTIDSFEL LKVIGKGSFG KVMQVRKRDT LRIYALKTIR KAHIVSRSEV THTLAERTVL AQVNCPFIVP LKFSFQSKEK LYLVLAFING GELFHHLQRE GKFNETRSRF YSAQLLLALE HLHSFNVIYR DLKPENILLD YAGNIALCDF GLCKLNMSNS DTTNTFCGTP EYLAPELLSG HGYTKCVDWW TLGVLLYEML TGLPPFYDEN TNEMYRKILT EPLRFPDGVR SEARSLLTGL LNRDPRQRLG VNGAQDIKNH PFFAKHINFT KLWNKQIQPP FKPAVASAID TSNFDEEFTN EVPLDSVVDD SHLSQTVQQ
Validated NT Sequence
atggctcacc accaccacca ccatatgggg caaattctta cgattgatag ctttgagctt cttaaagtta ttggcaaggg cagtttcggt aaagtcatgc aagtccgcaa gcgtgacacg ctccgaattt atgctctcaa aaccatccga aaagcccaca ttgtctcccg atccgaagtt acccacacct tggcagaacg taccgtcctc gcccaggtta actgcccttt tattgtgccc ttaaagttct ctttccagtc caaggaaaag ctgtacctgg tcttggcgtt catcaacgga ggagagttgt tccaccactt gcagagggag ggcaagttca acgagactag gtctaggttc tactcggccc aactactgct ggcgttggag catttgcact ccttcaatgt gatctacaga gatttgaagc ccgaaaacat tctgttagat tacgccggga atattgccct ttgtgatttt ggactgtgta aactcaacat gtccaactcg gacaccacaa ataccttctg tggtacacct gaatatcttg ctcctgaact tctttctggt cacggctata ctaaatgcgt tgactggtgg actcttggtg tcctcttgta cgaaatgctc actggtcttc ctccctttta cgacgaaaac accaacgaaa tgtaccgcaa gattctcacc gaacctttgc gcttccccga tggcgttcgt tctgaagcca ggtctcttct gaccggtttg cttaatcgag atcctcgcca aaggcttggc gtcaacggcg cccaggatat caagaatcac cccttctttg ccaaacacat caactttaca aaactctgga acaagcaaat ccaaccacct ttcaagcccg ctgtggcgag tgctattgac acttccaatt tcgatgagga gttcaccaac gaggtgcccc tcgactcggt tgttgacgac tcacatctgt cccagactgt gcaacagcaa
Expected Protein Sequence
MAHHHHHHGQ ILTIDSFELL KVIGKGSFGK VMQVRKRDTL RIYALKTIRK AHIVSRSEVT HTLAERTVLA QVNCPFIVPL KFSFQSKEKL YLVLAFINGG ELFHHLQREG KFNETRSRFY SAQLLLALEH LHSFNVIYRD LKPENILLDY AGNIALCDFG LCKLNMSNSD TTNTFCGTPE YLAPELLSGH GYTKCVDWWT LGVLLYEMLT GLPPFYDENT NEMYRKILTE PLRFPDGVRS EARSLLTGLL NRDPRQRLGV NGAQDIKNHP FFAKHINFTK LWNKQIQPPF KPAVASAIDT SNFDEEFTNE VPLDSVVDDS HLSQTVQQQF EGFSWS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgggcaaa ttcttacgat tgatagcttt gagcttctta aagttattgg caagggcagt ttcggtaaag tcatgcaagt ccgcaagcgt gacacgctcc gaatttatgc tctcaaaacc atccgaaaag cccacattgt ctcccgatcc gaagttaccc acaccttggc agaacgtacc gtcctcgccc aggttaactg cccttttatt gtgcccttaa agttctcttt ccagtccaag gaaaagctgt acctggtctt ggcgttcatc aacggaggag agttgttcca ccacttgcag agggagggca agttcaacga gactaggtct aggttctact cggcccaact actgctggcg ttggagcatt tgcactcctt caatgtgatc tacagagatt tgaagcccga aaacattctg ttagattacg ccgggaatat tgccctttgt gattttggac tgtgtaaact caacatgtcc aactcggaca ccacaaatac cttctgtggt acacctgaat atcttgctcc tgaacttctt tctggtcacg gctatactaa atgcgttgac tggtggactc ttggtgtcct cttgtacgaa atgctcactg gtcttcctcc cttttacgac gaaaacacca acgaaatgta ccgcaagatt ctcaccgaac ctttgcgctt ccccgatggc gttcgttctg aagccaggtc tcttctgacc ggtttgctta atcgagatcc tcgccaaagg cttggcgtca acggcgccca ggatatcaag aatcacccct tctttgccaa acacatcaac tttacaaaac tctggaacaa gcaaatccaa ccacctttca agcccgctgt ggcgagtgct attgacactt ccaatttcga tgaggagttc accaacgagg tgcccctcga ctcggttgtt gacgactcac atctgtccca gactgtgcaa cagcaattcg agggattctc ctggagttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at