SSGCID: Target Page

CrneC.18303.a

CMGC/CDK/CDK5 protein kinase (CMGC/CDK/CDK5 protein kinase, variant)

CENTER ID:	CrneC.18303.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.18303.a.B2.GE41409	Truncation 1		1	300

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_08022
UniProt:	T2BQ21

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNYVQLEKLG EGTYATVYKG RSRTTSEIVA LKEIHLDAEE GTPSTAIREI SLMKELKHVN IVRLHDVVHT ESKLVLIFEY CEQDLKRYMD IHGDRGALDL NTVKSFTHQL LQGIAFCHDH RVLHRDLKPQ NLLINKRGEL KIGDFGLARA FGVPVNTFSN EVVTLWYRAP DVLLGSRTYS TSIDIWSVGC IFAEMITGYP LFRGRDNADQ LVQIMKIVGT PSDATIAQIK LNSPEIQIKS PLAKHAKQPF QAIIPRAPRD AISLLEHLLQ FEPTRRYDAH QAMTHQYFTS GPIAPPTLHD NNPAVSSASS LALPPRVAAR ASQASAAVQA QQAAQAQAQA QAQAQAQAQA QAAQNAQIMA QQQQQQQQQQ QQYEMMMGQQ NMQGYYDPQA AAQMQAHQQA QAQAHAAQMQ QMAQQGYYMN PNGQHGHHH

NT Sequence

atgaattacg tccagcttga gaaacttggt gaaggtactt atgccactgt gtacaaaggc cgatcaagaa cgacttcaga aatcgtggca ctcaaggaga tccacctgga tgcggaagag gggacgccaa gtactgctat ccgagagatc agtcttatga aggagcttaa gcatgtaaac attgtccgcc tgcacgatgt tgttcatacc gaatctaagc ttgtcctcat tttcgagtac tgtgaacagg atttgaagag gtatatggat atccacggtg atcgcggtgc tttggatctg aacaccgtca agagctttac gcatcagctt cttcagggta ttgcgttttg tcatgatcat cgcgtcttac atcgagacct caagcctcaa aacctgttga tcaacaaacg aggagaattg aagattggtg atttcggttt ggcgagggcg tttggtgtgc cagtgaacac gttcagtaat gaagttgtga ccctttggta ccgtgcaccc gatgttttgc tcggttcaag gacgtatagc acaagtattg acatatggag tgtcggatgc atattcgcag agatgattac aggataccct ctctttagag gaagagataa tgccgatcaa ttggtgcaga tcatgaagat tgttggcaca ccaagcgatg ccaccattgc tcagatcaaa ttgaactctc ctgagattca aatcaaatcc cctttggcta aacatgccaa acagccgttc caggcaatta ttccccgagc tcctagggac gccatcagtc tgcttgaaca tcttcttcaa ttcgagccca ctcggcggta tgatgcacac caagctatga cccaccaata cttcacctct ggccctatcg cccctcccac attgcatgac aacaacccag ctgtttcatc ggcttcatcc cttgcccttc ctccaagggt ggctgcgcga gccagccagg cttcagctgc tgtgcaggct caacaagctg ctcaggctca agcgcaggcg caagcccaag ctcaagctca agctcaagct caggcggcac agaatgcgca gataatggct caacaacagc aacagcagca gcaacagcag cagcagtatg agatgatgat gggacagcag aatatgcagg ggtattacga ccctcaagca gccgcacaga tgcaagccca tcagcaggcc caagcgcaag cgcatgcagc ccagatgcag caaatggctc aacaagggta ctacatgaac cctaatggac agcatgggca tcaccattaa

Details for CrneC.18303.a.B2.GE41409

HARVESTED ON:	4/5/2017
SEQUENCED ON:	4/27/2017
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMN YVQLEKLGEG TYATVYKGRS RTTSEIVALK EIHLDAEEGT PSTAIREISL MKELKHVNIV RLHDVVHTES KLVLIFEYCE QDLKRYMDIH GDRGALDLNT VKSFTHQLLQ GIAFCHDHRV LHRDLKPQNL LINKRGELKI GDFGLARAFG VPVNTFSNEV VTLWYRAPDV LLGSRTYSTS IDIWSVGCIF AEMITGYPLF RGRDNADQLV QIMKIVGTPS DATIAQIKLN SPEIQIKSPL AKHAKQPFQA IIPRAPRDAI SLLEHLLQFE PTRRYDAHQA MTHQYFTSGP IXXXXIA

Validated NT Sequence

atggctcacc accaccacca ccatatgaat tacgtccagc ttgagaaact tggtgaaggt acttatgcca ctgtgtacaa aggccgatca agaacgactt cagaaatcgt ggcactcaag gagatccacc tggatgcgga agaggggacg ccaagtactg ctatccgaga gatcagtctt atgaaggagc ttaagcatgt aaacattgtc cgcctgcacg atgttgttca taccgaatct aagcttgtcc tcattttcga gtactgtgaa caggatttga agaggtatat ggatatccac ggtgatcgcg gtgctttgga tctgaacacc gtcaagagct ttacgcatca gcttcttcag ggtattgcgt tttgtcatga tcatcgcgtc ttacatcgag acctcaagcc tcaaaacctg ttgatcaaca aacgaggaga attgaagatt ggtgatttcg gtttggcgag ggcgtttggt gtgccagtga acacgttcag taatgaagtt gtgacccttt ggtaccgtgc acccgatgtt ttgctcggtt caaggacgta tagcacaagt attgacatat ggagtgtcgg atgcatattc gcagagatga ttacaggata ccctctcttt agaggaagag ataatgccga tcaattggtg cagatcatga agattgttgg cacaccaagc gatgccacca ttgctcagat caaattgaac tctcctgaga ttcaaatcaa atcccctttg gctaaacatg ccaaacagcc gttccaggca attattcccc gagctcctag ggacgccatc agtctgcttg aacatcttct tcaattcgag cccactcggc ggtatgatgc acaccaagct atgacccacc aatacttcac ctctggcccn atcnnnnnnn nnnacattgc atgactaagt gagtaaga

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt acgtccagct tgagaaactt ggtgaaggta cttatgccac tgtgtacaaa ggccgatcaa gaacgacttc agaaatcgtg gcactcaagg agatccacct ggatgcggaa gaggggacgc caagtactgc tatccgagag atcagtctta tgaaggagct taagcatgta aacattgtcc gcctgcacga tgttgttcat accgaatcta agcttgtcct cattttcgag tactgtgaac aggatttgaa gaggtatatg gatatccacg gtgatcgcgg tgctttggat ctgaacaccg tcaagagctt tacgcatcag cttcttcagg gtattgcgtt ttgtcatgat catcgcgtct tacatcgaga cctcaagcct caaaacctgt tgatcaacaa acgaggagaa ttgaagattg gtgatttcgg tttggcgagg gcgtttggtg tgccagtgaa cacgttcagt aatgaagttg tgaccctttg gtaccgtgca cccgatgttt tgctcggttc aaggacgtat agcacaagta ttgacatatg gagtgtcgga tgcatattcg cagagatgat tacaggatac cctctcttta gaggaagaga taatgccgat caattggtgc agatcatgaa gattgttggc acaccaagcg atgccaccat tgctcagatc aaattgaact ctcctgagat tcaaatcaaa tcccctttgg ctaaacatgc caaacagccg ttccaggcaa ttattccccg agctcctagg gacgccatca gtctgcttga acatcttctt caattcgagc ccactcggcg gtatgatgca caccaagcta tgacccacca atacttcacc tctggcccta tcgcccctcc cacattgcat gactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat