CrneC.18225.a
Diphosphomevalonate decarboxylase (MVD1) (CNAG_05125) (EC 4.1.1.33)
| CENTER ID: | CrneC.18225.a |
| ORGANISM: | Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487) |
| ASSOCIATED DISEASE: | Pneumonia-like illness |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrneC.18225.a.B1.GE41407 | full length | 1 | 395 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrneC.18225.a.B1.PS38378 | full length | 1 | 395 |
Structures
6XR5
DEPOSITED: 7/11/2020
DETERMINATION: XRay
CLONE: CrneC.18225.a.B1.GE41407
PROTEIN: CrneC.18225.a.B1.PS38378
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:CNAG_05125 |
| UniProt: | J9VRT5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVHEATASAP VNIACIKYWG KRDTRLILPT NSSLSVTLDQ DHLRSTTTSR ADASFEAGDR LWLNGREEAI KEGGRLAVCI KELRAWRKEM ETKDKNLPKL SEWPLRIASY NNFPTAAGLA SSASGLAALV ASLASLYSLP QSPSQLSLVA RQGSGSACRS LFGGFVAWRE GTDPAGSDSL AEEVAPREHW PEMHALICVV SDAKKGTSST SGMQKTVETS TLLQERLRVV PKRMDAISQA IKARDFAEFA KLTMADSNSF HAVCLDTAPP IFYLNDVSRA IIAVVEELNR AAGEIIAAYT FDAGPNAVIY TLEKNMPFVL GAIKRFFPTS EEFESPFQTG VRDLPEGFNT GVVREGGWEK GAVKGLIHTR VGDGPRVLEK EDSLLGENGV PKVLA
NT Sequence
atggtccacg aagccacagc atccgcgccc gtcaacatcg cgtgcatcaa atactggggc aagcgcgaca cccgcctcat cctccccacc aacagctctc tctccgtcac tcttgaccag gaccacctcc gctccaccac gacttcccgc gcggacgcgt ctttcgaggc cggagacagg ctctggctga acggcaggga agaggcaatc aaggagggcg gccgattggc tgtctgcatc aaggagctca gggcgtggag aaaagagatg gaaaccaagg acaagaactt gcccaagctc tccgagtggc cgctccggat tgcctcgtac aacaacttcc ccaccgctgc tggtctcgcc tcctcagcct cgggtcttgc cgccctcgtc gcatctctcg cctccctcta ctccctcccg cagtctccct cccagctctc cctcgtcgct cgccaaggct ctggctctgc ctgccggtct ctctttggag gcttcgtcgc ttggcgggaa ggtaccgatc ctgccggctc cgactcgctc gccgaggagg tcgcccctcg agagcactgg cccgagatgc acgctcttat ctgtgttgtc agcgatgcca agaagggcac gtcgtccacc tccggcatgc aaaagacagt agagacatct accctcttgc aagagcgtct ccgggtcgtc cccaagagga tggatgcgat ctctcaggct atcaaggctc gagactttgc cgaatttgcc aagctcacca tggccgatag caactctttc cacgccgttt gtctcgacac cgcccctccc atcttctacc tcaatgacgt ctctcgagcc atcatcgccg ttgtcgaaga gctcaaccgt gccgccggcg agatcatcgc cgcctacaca tttgacgccg gacccaacgc cgttatctac acactcgaga agaacatgcc attcgtcctc ggcgcaatca agaggttctt ccctaccagc gaagagtttg agtccccctt ccagacaggg gtgcgagatt tgcctgaagg gttcaacact ggtgtggtca gggaaggagg atgggagaag ggtgcggtca agggtttgat ccacaccagg gtcggtgacg gtcccagagt tttggaaaag gaggacagtt tattggggga gaatggtgtg cccaaagttc ttgcttag
Details for CrneC.18225.a.B1.GE41407
| HARVESTED ON: | 4/5/2017 |
| SEQUENCED ON: | 4/27/2017 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMV HEATASAPVN IACIKYWGKR DTRLILPTNS SLSVTLDQDH LRSTTTSRAD ASFEAGDRLW LNGREEAIKE GGRLAVCIKE LRAWRKEMET KDKNLPKLSE WPLRIASYNN FPTAAGLASS ASGLAALVAS LASLYSLPQS PSQLSLVARQ GSGSACRSLF GGFVAWREGT DPAGSDSLAE EVAPREHWPE MHALICVVSD AKKGTSSTSG MQKTVETSTL LQERLRVVPK RMDAISQAIK ARDFAEFAKL TMADSNSFHA VCLDTAPPIF YLNDVSRAII AVVEELNRAA GEIIAAYTFD AGPNAVIYTL EKNMPFVLGA IKRFFPTSEE FESPFQTGVR DLPEGFNTGV VREGGWEKGA VKGLIHTRVG DGPRVLEKED SLLGE
Validated NT Sequence
atggctcacc accaccacca ccatatggtc cacgaagcca cagcatccgc gcccgtcaac atcgcgtgca tcaaatactg gggcaagcgc gacacccgcc tcatcctccc caccaacagc tctctctccg tcactcttga ccaggaccac ctccgctcca ccacgacttc ccgcgcggac gcgtctttcg aggccggaga caggctctgg ctgaacggca gggaagaggc aatcaaggag ggcggccgat tggctgtctg catcaaggag ctcagggcgt ggagaaaaga gatggaaacc aaggacaaga acttgcccaa gctctccgag tggccgctcc ggattgcctc gtacaacaac ttccccaccg ctgctggtct cgcctcctca gcctcgggtc ttgccgccct cgtcgcatct ctcgcctccc tctactccct cccgcagtct ccctcccagc tctccctcgt cgctcgccaa ggctctggct ctgcctgccg gtctctcttt ggaggcttcg tcgcttggcg ggaaggtacc gatcctgccg gctccgactc gctcgccgag gaggtcgccc ctcgagagca ctggcccgag atgcacgctc ttatctgtgt tgtcagcgat gccaagaagg gcacgtcgtc cacctccggc atgcaaaaga cagtagagac atctaccctc ttgcaagagc gtctccgggt cgtccccaag aggatggatg cgatctctca ggctatcaag gctcgagact ttgccgaatt tgccaagctc accatggccg atagcaactc tttccacgcc gtttgtctcg acaccgcccc tcccatcttc tacctcaatg acgtctctcg agccatcatc gccgttgtcg aagagctcaa ccgtgccgcc ggcgagatca tcgccgccta cacatttgac gccggaccca acgccgttat ctacacactc gagaagaaca tgccattcgt cctcggcgca atcaagaggt tcttccctac cagcgaagag tttgagtccc ccttccagac aggggtgcga gatttgcctg aagggttcaa cactggtgtg gtcagggaag gaggatggga gaagggtgcg gtcaagggtt tgatccacac cagggtcggt gacggtccca gagttttgga aaaggaggac agtttattgg gggagaa
Expected Protein Sequence
MAHHHHHHMV HEATASAPVN IACIKYWGKR DTRLILPTNS SLSVTLDQDH LRSTTTSRAD ASFEAGDRLW LNGREEAIKE GGRLAVCIKE LRAWRKEMET KDKNLPKLSE WPLRIASYNN FPTAAGLASS ASGLAALVAS LASLYSLPQS PSQLSLVARQ GSGSACRSLF GGFVAWREGT DPAGSDSLAE EVAPREHWPE MHALICVVSD AKKGTSSTSG MQKTVETSTL LQERLRVVPK RMDAISQAIK ARDFAEFAKL TMADSNSFHA VCLDTAPPIF YLNDVSRAII AVVEELNRAA GEIIAAYTFD AGPNAVIYTL EKNMPFVLGA IKRFFPTSEE FESPFQTGVR DLPEGFNTGV VREGGWEKGA VKGLIHTRVG DGPRVLEKED SLLGENGVPK VLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtcc acgaagccac agcatccgcg cccgtcaaca tcgcgtgcat caaatactgg ggcaagcgcg acacccgcct catcctcccc accaacagct ctctctccgt cactcttgac caggaccacc tccgctccac cacgacttcc cgcgcggacg cgtctttcga ggccggagac aggctctggc tgaacggcag ggaagaggca atcaaggagg gcggccgatt ggctgtctgc atcaaggagc tcagggcgtg gagaaaagag atggaaacca aggacaagaa cttgcccaag ctctccgagt ggccgctccg gattgcctcg tacaacaact tccccaccgc tgctggtctc gcctcctcag cctcgggtct tgccgccctc gtcgcatctc tcgcctccct ctactccctc ccgcagtctc cctcccagct ctccctcgtc gctcgccaag gctctggctc tgcctgccgg tctctctttg gaggcttcgt cgcttggcgg gaaggtaccg atcctgccgg ctccgactcg ctcgccgagg aggtcgcccc tcgagagcac tggcccgaga tgcacgctct tatctgtgtt gtcagcgatg ccaagaaggg cacgtcgtcc acctccggca tgcaaaagac agtagagaca tctaccctct tgcaagagcg tctccgggtc gtccccaaga ggatggatgc gatctctcag gctatcaagg ctcgagactt tgccgaattt gccaagctca ccatggccga tagcaactct ttccacgccg tttgtctcga caccgcccct cccatcttct acctcaatga cgtctctcga gccatcatcg ccgttgtcga agagctcaac cgtgccgccg gcgagatcat cgccgcctac acatttgacg ccggacccaa cgccgttatc tacacactcg agaagaacat gccattcgtc ctcggcgcaa tcaagaggtt cttccctacc agcgaagagt ttgagtcccc cttccagaca ggggtgcgag atttgcctga agggttcaac actggtgtgg tcagggaagg aggatgggag aagggtgcgg tcaagggttt gatccacacc agggtcggtg acggtcccag agttttggaa aaggaggaca gtttattggg ggagaatggt gtgcccaaag ttcttgcttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for CrneC.18225.a.B1.PS38378
| PURIFICATION DATe: | 11/2/2017 |
| CONCENTRATION: | 62.37mg/ml |
| OBSERVED MW: | 42kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV HEATASAPVN IACIKYWGKR DTRLILPTNS SLSVTLDQDH LRSTTTSRAD ASFEAGDRLW LNGREEAIKE GGRLAVCIKE LRAWRKEMET KDKNLPKLSE WPLRIASYNN FPTAAGLASS ASGLAALVAS LASLYSLPQS PSQLSLVARQ GSGSACRSLF GGFVAWREGT DPAGSDSLAE EVAPREHWPE MHALICVVSD AKKGTSSTSG MQKTVETSTL LQERLRVVPK RMDAISQAIK ARDFAEFAKL TMADSNSFHA VCLDTAPPIF YLNDVSRAII AVVEELNRAA GEIIAAYTFD AGPNAVIYTL EKNMPFVLGA IKRFFPTSEE FESPFQTGVR DLPEGFNTGV VREGGWEKGA VKGLIHTRVG DGPRVLEKED SLLGE
Validated NT Sequence
atggctcacc accaccacca ccatatggtc cacgaagcca cagcatccgc gcccgtcaac atcgcgtgca tcaaatactg gggcaagcgc gacacccgcc tcatcctccc caccaacagc tctctctccg tcactcttga ccaggaccac ctccgctcca ccacgacttc ccgcgcggac gcgtctttcg aggccggaga caggctctgg ctgaacggca gggaagaggc aatcaaggag ggcggccgat tggctgtctg catcaaggag ctcagggcgt ggagaaaaga gatggaaacc aaggacaaga acttgcccaa gctctccgag tggccgctcc ggattgcctc gtacaacaac ttccccaccg ctgctggtct cgcctcctca gcctcgggtc ttgccgccct cgtcgcatct ctcgcctccc tctactccct cccgcagtct ccctcccagc tctccctcgt cgctcgccaa ggctctggct ctgcctgccg gtctctcttt ggaggcttcg tcgcttggcg ggaaggtacc gatcctgccg gctccgactc gctcgccgag gaggtcgccc ctcgagagca ctggcccgag atgcacgctc ttatctgtgt tgtcagcgat gccaagaagg gcacgtcgtc cacctccggc atgcaaaaga cagtagagac atctaccctc ttgcaagagc gtctccgggt cgtccccaag aggatggatg cgatctctca ggctatcaag gctcgagact ttgccgaatt tgccaagctc accatggccg atagcaactc tttccacgcc gtttgtctcg acaccgcccc tcccatcttc tacctcaatg acgtctctcg agccatcatc gccgttgtcg aagagctcaa ccgtgccgcc ggcgagatca tcgccgccta cacatttgac gccggaccca acgccgttat ctacacactc gagaagaaca tgccattcgt cctcggcgca atcaagaggt tcttccctac cagcgaagag tttgagtccc ccttccagac aggggtgcga gatttgcctg aagggttcaa cactggtgtg gtcagggaag gaggatggga gaagggtgcg gtcaagggtt tgatccacac cagggtcggt gacggtccca gagttttgga aaaggaggac agtttattgg gggagaa
Expressed Protein Sequence
MAHHHHHHMV HEATASAPVN IACIKYWGKR DTRLILPTNS SLSVTLDQDH LRSTTTSRAD ASFEAGDRLW LNGREEAIKE GGRLAVCIKE LRAWRKEMET KDKNLPKLSE WPLRIASYNN FPTAAGLASS ASGLAALVAS LASLYSLPQS PSQLSLVARQ GSGSACRSLF GGFVAWREGT DPAGSDSLAE EVAPREHWPE MHALICVVSD AKKGTSSTSG MQKTVETSTL LQERLRVVPK RMDAISQAIK ARDFAEFAKL TMADSNSFHA VCLDTAPPIF YLNDVSRAII AVVEELNRAA GEIIAAYTFD AGPNAVIYTL EKNMPFVLGA IKRFFPTSEE FESPFQTGVR DLPEGFNTGV VREGGWEKGA VKGLIHTRVG DGPRVLEKED SLLGENGVPK VLA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTCC ACGAAGCCAC AGCATCCGCG CCCGTCAACA TCGCGTGCAT CAAATACTGG GGCAAGCGCG ACACCCGCCT CATCCTCCCC ACCAACAGCT CTCTCTCCGT CACTCTTGAC CAGGACCACC TCCGCTCCAC CACGACTTCC CGCGCGGACG CGTCTTTCGA GGCCGGAGAC AGGCTCTGGC TGAACGGCAG GGAAGAGGCA ATCAAGGAGG GCGGCCGATT GGCTGTCTGC ATCAAGGAGC TCAGGGCGTG GAGAAAAGAG ATGGAAACCA AGGACAAGAA CTTGCCCAAG CTCTCCGAGT GGCCGCTCCG GATTGCCTCG TACAACAACT TCCCCACCGC TGCTGGTCTC GCCTCCTCAG CCTCGGGTCT TGCCGCCCTC GTCGCATCTC TCGCCTCCCT CTACTCCCTC CCGCAGTCTC CCTCCCAGCT CTCCCTCGTC GCTCGCCAAG GCTCTGGCTC TGCCTGCCGG TCTCTCTTTG GAGGCTTCGT CGCTTGGCGG GAAGGTACCG ATCCTGCCGG CTCCGACTCG CTCGCCGAGG AGGTCGCCCC TCGAGAGCAC TGGCCCGAGA TGCACGCTCT TATCTGTGTT GTCAGCGATG CCAAGAAGGG CACGTCGTCC ACCTCCGGCA TGCAAAAGAC AGTAGAGACA TCTACCCTCT TGCAAGAGCG TCTCCGGGTC GTCCCCAAGA GGATGGATGC GATCTCTCAG GCTATCAAGG CTCGAGACTT TGCCGAATTT GCCAAGCTCA CCATGGCCGA TAGCAACTCT TTCCACGCCG TTTGTCTCGA CACCGCCCCT CCCATCTTCT ACCTCAATGA CGTCTCTCGA GCCATCATCG CCGTTGTCGA AGAGCTCAAC CGTGCCGCCG GCGAGATCAT CGCCGCCTAC ACATTTGACG CCGGACCCAA CGCCGTTATC TACACACTCG AGAAGAACAT GCCATTCGTC CTCGGCGCAA TCAAGAGGTT CTTCCCTACC AGCGAAGAGT TTGAGTCCCC CTTCCAGACA GGGGTGCGAG ATTTGCCTGA AGGGTTCAAC ACTGGTGTGG TCAGGGAAGG AGGATGGGAG AAGGGTGCGG TCAAGGGTTT GATCCACACC AGGGTCGGTG ACGGTCCCAG AGTTTTGGAA AAGGAGGACA GTTTATTGGG GGAGAATGGT GTGCCCAAAG TTCTTGCTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT