CrneC.17951.a

Aspartokinase (EC 2.7.2.4)

CENTER ID: CrneC.17951.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.17951.a.B2.GE41406 Truncation 1 212 615
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_04347
UniProt: J9VS37

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSASTPTAP YLEDLVRNSL DQNLPWVVQK YGGTSVGKSL DNITKIVGSY IDNGSKVAIV CSARSTQTKS LGTTNLLLQA SREALQPAMS SSGDGRSGSM SGTATPFYPK RVGSGFFGKD QSTSMTSSVS SLSQLESQLG RSGSPSPFQS SSSRSPPRSP ATPSQDSSIS QEPAFHATVD LIKKGHLEAA RASLKGGPLR DELEEEIERD CESLRSFLYA AQIIDEISPR SQDSIVGTGE RLACKIVAAA LRDRGVDSEL VVLDNIVDAS MSAASEAVSV DAGDQGVAQL GQEFYDQLSF RLGERLRECG QRVPVVTGYF GPVPGSLLAQ IGRGYTDLCA ALCAVGLKAS ELQVWKEVDG IFTADPRKVP SARLVPIITP DEAAELTYYG SEVIHPFTME QVIRARIPIR IKNVENPSGA GTVIYPDVGF PRGLDTEPPK AERIVEGVDE RMPTAVTIKD EIIVLNIHSN RKTLSHGFLA RIFGTLDRAG VVVDLISTSE VHVSMAMQDF LNRKRLERLV KDLEKIGEVT VSKDMAILSL VGRNMRNAIG SAGLMFASLA RAMINIEMIS QGASEINISC VIENKDAIKA LNVIHESCLS YPRSPATEMA GLQLQ
NT Sequence
atgtcctcag catccacacc caccgctcca taccttgagg acctcgtccg caactcgcta gaccaaaacc tgccctgggt cgtccagaag tacggcggga cgtcggttgg taaaagcctg gacaacatca ccaaaattgt cgggtcatat atcgacaatg gctctaaagt tgctatcgtt tgctcggctc gttctacgca gactaagtca ctcggtacca cgaatctcct ccttcaagct tcccgagaag ctcttcagcc ggcaatgtct tcttcaggtg acggccgttc cggctctatg tctggtaccg ccaccccttt ctaccccaaa cgtgttggtt caggcttttt tggcaaggac caatctactt ccatgacgtc ttccgtttcg tccctctcgc agctcgagtc tcaacttgga aggtccggca gccccagccc ttttcaaagc agttctagcc gatctcctcc cagaagtccg gccacaccct ctcaagattc atccatcagt caggaacccg cgttccatgc gactgtagat ctcatcaaga agggccattt ggaagctgct cgggcatcat tgaagggagg tccattgcgt gatgagcttg aagaagagat tgaaagggat tgtgaaagcc tcagaagttt cctatatgct gctcagatca ttgacgaaat cagcccaaga agccaagatt ccatcgtcgg taccggagaa cggttggcgt gtaagatcgt cgctgctgcc ttgagagata ggggcgtgga ctctgagctc gttgttctcg acaacatcgt tgatgcgtcc atgtctgctg ccagcgaggc agtttctgtt gatgctggtg atcagggagt cgcgcaactt ggccaagaat tctatgacca gctttcgttc agattgggtg agagactgag agaatgtggc caaagagttc ctgttgtgac tggctacttt ggccccgtcc ccggctccct tcttgcgcaa atcggccgtg gatatacgga cctttgtgca gctctctgcg ctgttggttt gaaagcctcc gaattgcaag tatggaagga agtcgatggt attttcaccg cggaccctcg aaaagtacct tctgcccgtc tagtacccat catcaccccc gacgaagctg cggagctcac ctattacggc tcagaagtta tccatccttt cactatggag caagttatcc gagcgaggat tcctatcagg atcaagaatg ttgagaatcc ctcgggcgct ggtacagtta tctaccctga cgtcggcttc ccccggggct tggacactga acctcctaag gccgaaagga tcgttgaagg tgttgacgag agaatgccga ctgctgtgac catcaaggat gagattattg tcctcaatat tcactccaat agaaagacat tatctcacgg cttcctcgcc aggatcttcg ggacattaga cagggccggt gtcgttgttg atctgatcag caccagtgaa gtgcatgtat ccatggctat gcaagacttc ctcaaccgaa agcgcctgga gcgtcttgtt aaggaccttg aaaaaatcgg cgaggtcact gtttcaaagg acatggctat cctctctctt gtcggacgta atatgaggaa tgccatcgga agtgctggtt taatgtttgc cagtttggct cgggcgatga tcaacattga gatgatcagt cagggtgcga gcgagatcaa tatcagctgt gtgattgaga acaaggatgc tatcaaggcc ctcaatgtta tccacgaatc ttgcctatct tatcccagat cccctgccac ggagatggcg ggtttacagc ttcaatag
Details for CrneC.17951.a.B2.GE41406
HARVESTED ON: 4/5/2017
SEQUENCED ON: 4/27/2017
EXPECTED MW: 45kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHES LRSFLYAAQI IDEISPRSQD SIVGTGERLA CKIVAAALRD RGVDSELVVL DNIVDASMSA ASEAVSVDAG DQGVAQLGQE FYDQLSFRLG ERLRECGQRV PVVTGYFGPV PGSLLAQIGR GYTDLCAALC AVGLKASELQ VWKEVDGIFT ADPRKVPSAR LVPIITPDEA AELTYYGSEV IHPFTMEQVI RARIPIRIKN VENPSGAGTV IYPDVGFPRG LDTEPPKAER IVEGVDERMP TAVTIKDEII VLNIHSNRKT LSHGFLARIF GTLDRAGVVV DLISTSEVHV SMAMQDFLNR KRLERLVKDL EKIGEVTVSK DMAILSLVGR NMRNAIGSAG LMFASLARAM INIEMISQGA SEINISCVIE NKDAIKALNV IHESCLSYPR SPATEMAGLQ LQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaaagcc tcagaagttt cctatatgct gctcagatca ttgacgaaat cagcccaaga agccaagatt ccatcgtcgg taccggagaa cggttggcgt gtaagatcgt cgctgctgcc ttgagagata ggggcgtgga ctctgagctc gttgttctcg acaacatcgt tgatgcgtcc atgtctgctg ccagcgaggc agtttctgtt gatgctggtg atcagggagt cgcgcaactt ggccaagaat tctatgacca gctttcgttc agattgggtg agagactgag agaatgtggc caaagagttc ctgttgtgac tggctacttt ggccccgtcc ccggctccct tcttgcgcaa atcggccgtg gatatacgga cctttgtgca gctctctgcg ctgttggttt gaaagcctcc gaattgcaag tatggaagga agtcgatggt attttcaccg cggaccctcg aaaagtacct tctgcccgtc tagtacccat catcaccccc gacgaagctg cggagctcac ctattacggc tcagaagtta tccatccttt cactatggag caagttatcc gagcgaggat tcctatcagg atcaagaatg ttgagaatcc ctcgggcgct ggtacagtta tctaccctga cgtcggcttc ccccggggct tggacactga acctcctaag gccgaaagga tcgttgaagg tgttgacgag agaatgccga ctgctgtgac catcaaggat gagattattg tcctcaatat tcactccaat agaaagacat tatctcacgg cttcctcgcc aggatcttcg ggacattaga cagggccggt gtcgttgttg atctgatcag caccagtgaa gtgcatgtat ccatggctat gcaagacttc ctcaaccgaa agcgcctgga gcgtcttgtt aaggaccttg aaaaaatcgg cgaggtcact gtttcaaagg acatggctat cctctctctt gtcggacgta atatgaggaa tgccatcgga agtgctggtt taatgtttgc cagtttggct cgggcgatga tcaacattga gatgatcagt cagggtgcga gcgagatcaa tatcagctgt gtgattgaga acaaggatgc tatcaaggcc ctcaatgtta tccacgaatc ttgcctatct tatcccagat cccctgccac ggagatggcg ggtttacagc ttcaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat