CrneC.10384.a

CMGC/CDK/CDK8 protein kinase

CENTER ID: CrneC.10384.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.10384.a.B3.GE41405 Truncation 2 128 424
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_06086
UniProt: J9VVM2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATIPGGGII MDPMHFYRAR RDKERRGVLK TYKILGFISS GTYGRVYKAV LLPPPKTASA KSALPSSTRA ALSLPKDKLP SPSFTEDSDP LNNPEMCMRP GDRPAKKGDV FAIKKFKPDK EGDVLTYAGI SQSGAREIML NRELHHRNLV SLREVILEDK SIYMVFEYAE HDFLQIIHYH SQTARASIPP STLRRLLHQL LCGVHFLHSN FVLHRDLKPA NILVTSQGVV KIGDLGLARL WHKPLAQQGL YGGDKVVVTI WYRAPELILG AKHYTAAVDI WAVGCIYAEL LSLRPIFKGD EARMDGKKSL PFQRDQMGKI CEVLGPVKPE QWPGIVHMPE YRTYQATGPY PHSNPLAPWY HARSNSPEGY DILIKMFEWD PARRITARDA LRHPWFQEEG GVDTKSVFEG SSISYPTRRV THEDNGDAKM GSLPQSMAGG RLPSSSNFRP ASGNIAQPAA RKKARI
NT Sequence
atggcaacga tcccaggcgg aggtattata atggacccaa tgcactttta tcgtgcaaga agggataagg agagaagagg cgtcttgaag acatataaga ttctgggatt catatcttcc ggaacttatg gaagagtcta caaagcagtg ctcctcccac cacccaaaac agcctctgcc aaaagcgcct tgcccagctc gactcgagca gccttatcat tacctaaaga caagttacca tcgcctagct tcaccgaaga cagcgaccct ctcaataatc ccgaaatgtg tatgcgccca ggcgaccgtc ccgccaaaaa gggcgacgtc ttcgcgataa agaagttcaa gcctgataag gaaggcgatg tactgaccta cgccggtatc agtcaaagtg gagcgagaga aattatgctc aaccgtgaac tccaccatcg caatctggta tcgctgcgtg aagtcatatt agaagacaag tctatttata tggtttttga gtatgcggaa catgattttc tacaaattat tcactaccat tctcaaactg cacgagcttc tatcccacct tccacacttc gccgccttct tcaccagctt ctctgcggag tgcactttct tcactctaat tttgtcctcc accgtgatct caaaccggct aacattttgg ttaccagcca aggagttgtt aagataggtg atctcgggct ggcacgattg tggcataaac cattagcgca acagggtctc tatggtggtg ataaggtcgt ggtgactatc tggtacagag cgccagagtt aatattggga gccaagcact acactgccgc tgtcgatata tgggcggtag gttgtatcta tgcggagctg ctctctcttc gccctatctt caaaggcgac gaggctagaa tggacggcaa gaagtctttg ccattccagc gcgaccagat gggcaaaatc tgtgaagttc ttggtcctgt caagcccgaa caatggccag gtatagtcca catgcctgaa taccgcactt accaagccac tggtccttat ccccactcca atcctcttgc accatggtat catgcccgct caaactcgcc tgaaggatac gatattctca tcaagatgtt cgaatgggac ccagctcgta ggataacggc aagggatgca ttgaggcatc cttggttcca ggaagaaggg ggagtcgata ccaaaagtgt gttcgaaggc agctcgatct catatcccac aagaagggtg acgcatgagg ataacggtga tgctaagatg ggatctctac ctcaatcgat ggctggaggc aggctgccat caagtagcaa tttcaggccc gcaagtggca atattgctca accggcggct aggaagaagg cgagaattta a
Details for CrneC.10384.a.B3.GE41405
HARVESTED ON: 4/5/2017
SEQUENCED ON: 4/27/2017
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM AGISQSGARE IMLNRELHHR NLVSLREVIL EDKSIYMVFE YAEHDFLQII HYHSQTARAS IPPSTLRRLL HQLLCGVHFL HSNFVLHRDL KPANILVTSQ GVVKIGDLGL ARLWHKPLAQ QGLYGGDKVV VTIWYRAPEL ILGAKHYTAA VDIWAVGCIY AELLSLRPIF KGDEARMDGK KSLPFQRDQM GKICEVLGPV KPEQWPGIVH MPEYRTYQAT GPYPHSNPLA PWYHARSNSP EGYDILIKMF EWDPARRITA RDALRHPWFQ EEGGVDTKSV FEGSSISYPT RRVTHED
Validated NT Sequence
atggctcacc accaccacca ccaccatatg gccggtatca gtcaaagtgg agcgagagaa attatgctca accgtgaact ccaccatcgc aatctggtat cgctgcgtga agtcatatta gaagacaagt ctatttatat ggtttttgag tatgcggaac atgattttct acaaattatt cactaccatt ctcaaactgc acgagcttct atcccacctt ccacacttcg ccgccttctt caccagcttc tctgcggagt gcactttctt cactctaatt ttgtcctcca ccgtgatctc aaaccggcta acattttggt taccagccaa ggagttgtta agataggtga tctcgggctg gcacgattgt ggcataaacc attagcgcaa cagggtctct atggtggtga taaggtcgtg gtgactatct ggtacagagc gccagagtta atattgggag ccaagcacta cactgccgct gtcgatatat gggcggtagg ttgtatctat gcggagctgc tctctcttcg ccctatcttc aaaggcgacg aggctagaat ggacggcaag aagtctttgc cattccagcg cgaccagatg ggcaaaatct gtgaagttct tggtcctgtc aagcccgaac aatggccagg tatagtccac atgcctgaat accgcactta ccaagccact ggtccttatc cccactccaa tcctcttgca ccatggtatc atgcccgctc aaactcgcct gaaggatacg atattctcat caagatgttc gaatgggacc cagctcgtag gataacggca agggatgcat tgaggcatcc ttggttccag gaagaagggg gagtcgatac caaaagtgtg ttcgaaggca gctcgatctc atatcccaca agaagggtga cgcatgagga ttaagtg
Expected Protein Sequence
MAHHHHHHAG ISQSGAREIM LNRELHHRNL VSLREVILED KSIYMVFEYA EHDFLQIIHY HSQTARASIP PSTLRRLLHQ LLCGVHFLHS NFVLHRDLKP ANILVTSQGV VKIGDLGLAR LWHKPLAQQG LYGGDKVVVT IWYRAPELIL GAKHYTAAVD IWAVGCIYAE LLSLRPIFKG DEARMDGKKS LPFQRDQMGK ICEVLGPVKP EQWPGIVHMP EYRTYQATGP YPHSNPLAPW YHARSNSPEG YDILIKMFEW DPARRITARD ALRHPWFQEE GGVDTKSVFE GSSISYPTRR VTHED
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccggta tcagtcaaag tggagcgaga gaaattatgc tcaaccgtga actccaccat cgcaatctgg tatcgctgcg tgaagtcata ttagaagaca agtctattta tatggttttt gagtatgcgg aacatgattt tctacaaatt attcactacc attctcaaac tgcacgagct tctatcccac cttccacact tcgccgcctt cttcaccagc ttctctgcgg agtgcacttt cttcactcta attttgtcct ccaccgtgat ctcaaaccgg ctaacatttt ggttaccagc caaggagttg ttaagatagg tgatctcggg ctggcacgat tgtggcataa accattagcg caacagggtc tctatggtgg tgataaggtc gtggtgacta tctggtacag agcgccagag ttaatattgg gagccaagca ctacactgcc gctgtcgata tatgggcggt aggttgtatc tatgcggagc tgctctctct tcgccctatc ttcaaaggcg acgaggctag aatggacggc aagaagtctt tgccattcca gcgcgaccag atgggcaaaa tctgtgaagt tcttggtcct gtcaagcccg aacaatggcc aggtatagtc cacatgcctg aataccgcac ttaccaagcc actggtcctt atccccactc caatcctctt gcaccatggt atcatgcccg ctcaaactcg cctgaaggat acgatattct catcaagatg ttcgaatggg acccagctcg taggataacg gcaagggatg cattgaggca tccttggttc caggaagaag ggggagtcga taccaaaagt gtgttcgaag gcagctcgat ctcatatccc acaagaaggg tgacgcatga ggattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat