CrneC.10317.c

CMGC/CLK protein kinase

CENTER ID: CrneC.10317.c
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.10317.c.B2.GE41403 Truncation 1 404 748
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_00683
UniProt: J9VEN9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTSSHLSHA THLPRPADTL PRSPYPHDTH ESPLPPPSFL HPFPLPPRAS IPSILGPPLH SAPPPPQPPS SSAKPLRPHS ASLPLPHPYY PLPLPHPHAP SDHRVLRSPA PQYLWPLPPP LPSIDSLRRP ARHHPHSLSH SHSDSPSLEQ PRPYYDPAPY RDSYPSYPSN HPSSPYLPPP TPSAYAYRAP SPFRIPPPLT FRYSRSPPPL DTMPPRRKAE PSNAATVAPS NAPAATGALA PAGTRARRGA NGKGWTTEHT YEPNGQRKEV IVIDDSASPM VQPVRKRTRA QVAAEQAAQQ QQLHQAYAST NAGYSDTVSI SNGHGSVAST AGTAAGGNTK KRKLDEDQGK KTKAKVASTA TSASVQTTGT WQSTQQAAPA KAAQYQKAPA QPAQQQGPPP WDDSEGHYIV KPDDVIGGRY KIVRLLGQGT FGKVVEARHI ETRRKVAIKV IRAVQKYREA SKIEIRVLET LRKHDPRNDN KCIHLDEYFD FRNHPCLVSE LYGMSVFDFL KQNGFQPFPD KHIQDFAKSL LRSVSYLHSL KLVHTDLKPE NILLCSNEAR LAGPRVRNAR SKSILKNTEI RLIDFGSATF ESEYHSSVVS TRHYRAPEII LGLSWSYPCD MFSIGCILVE FYIGNALFQT HDNLEHLAMM EVVMGKFSQR MIEKGKSKKP EYFKGNKIDF PNSTVSKASR KYVKSMQSLK QVIDPANRHQ QLFLDLCTRL LEHDPDVRIK VQDALRHPYL TEPIPEPA
NT Sequence
atgagcacca gcagccacct ctcccacgcc acccacctcc cccgccccgc agacaccctc ccccgcagcc cgtacccgca cgacacccac gagtcgccgc tccccccgcc gtccttcctc cacccgtttc ccctgccgcc ccgggcgtcc atcccctcca tcctcggccc cccgctgcac tctgcgccgc cgccgccgca gcccccgagc tcgtccgcga agcccctccg cccgcacagt gcgtcgctgc ccctccccca cccgtactac cctctccccc tcccccaccc ccacgccccg tccgaccacc gcgttctccg ctcccccgcc ccgcagtatc tctggccgct gcccccgccc ctgccctcca tcgactccct ccggcgcccg gcccgccatc acccgcactc cctctcccac tcccactccg actcgcccag cctggagcag ccgcggccgt actacgatcc cgccccgtac cgcgactcgt atccctccta cccatccaac catcccagct cgccctacct cccccctccg actccttccg cgtacgccta ccgcgccccc tccccgttcc ggatccctcc gccgctgacg ttcaggtatt ctcgctcacc gccgccgctc gacaccatgc ccccacgcag aaaggccgaa ccgtccaacg cagccactgt ggctccgtcc aatgcaccag cggctaccgg cgcgctggcc cctgctggca cgagagcgag aagaggtgcc aacggcaagg gatggacaac tgagcacacc tacgagccca acggtcagcg caaggaggtc atcgtcattg acgactctgc aagcccaatg gtgcagcctg tgaggaagag gacgcgcgca caggtcgcag ccgagcaagc agcccagcag cagcaattac atcaggccta tgcgagcacg aacgccggtt actcagatac tgtgagcata tcaaacgggc acgggagcgt ggccagcacg gctggtacag cagcgggagg aaacacgaag aagagaaagt tggatgagga ccagggaaag aagacaaaag ccaaggttgc gagtacggcg acgtcggcat cggtacagac gactgggacc tggcaatcga cccaacaggc tgccccagcc aaggccgccc agtaccagaa agcccctgcc cagccagctc aacagcaggg tccaccaccg tgggatgact ctgaaggtca ttacattgtc aagcctgacg atgttatcgg cggtagatac aaaattgtca ggttactggg tcagggaacg tttggcaagg tggttgaagc cagacatatt gagacacggc gtaaagtcgc gatcaaggtt atccgagccg tgcaaaagta tcgtgaggcg agcaaaattg agattagagt gcttgagacg ctcagaaagc atgacccacg gaatgataac aaatgtatac atctcgacga atactttgac ttccggaacc acccgtgcct cgtctccgag ttgtacggga tgagtgtatt tgacttttta aagcaaaacg gtttccaacc attcccggat aagcacattc aagactttgc aaagagttta ttaagaagcg tgtcctactt gcattctctc aagctcgtac acaccgacct caagccggag aatattcttc tatgttcaaa cgaggcgaga ttagcaggcc ccagagttcg aaatgctcgt tcaaaatcta tcctcaagaa cactgaaatc cgtcttatcg attttggttc tgccactttt gaatctgaat atcattcctc tgtcgtctct actagacatt atcgtgcccc tgaaatcata cttggtcttt catggtctta tccttgtgac atgttcagta taggctgtat cctggtagag ttctacattg gaaacgcgct ttttcagaca cacgacaatt tggaacattt ggcgatgatg gaggttgtga tgggtaagtt tagccagagg atgattgaga agggaaaaag taagaagcca gagtatttca aaggcaacaa gatagatttt cccaactcta cggtttccaa ggcgagcaga aaatatgtga agagcatgca aagcctcaag caagtaatcg atcctgccaa cagacatcaa cagttgttcc ttgacctttg tacaagattg ctagagcatg atccggatgt tcggatcaag gtgcaggacg ctttacgaca tccgtatttg accgaaccta ttccagagcc cgcataa
Details for CrneC.10317.c.B2.GE41403
HARVESTED ON: 4/5/2017
SEQUENCED ON: 4/27/2017
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS EGHYIVKPDD VIGGRYKIVR LLGQGTFGKV VEARHIETRR KVAIKVIRAV QKYREASKIE IRVLETLRKH DPRNDNKCIH LDEYFDFRNH PCLVSELYGM SVFDFLKQNG FQPFPDKHIQ DFAKSLLRSV SYLHSLKLVH TDLKPENILL CSNEARLAGP RVRNARSKSI LKNTEIRLID FGSATFESEY HSSVVSTRHY RAPEIILGLS WSYPCDMFSI GCILVEFYIG NALFQTHDNL EHLAMMEVVM GKFSQRMIEK GKSKKPEYFK GNKIDFPNST VSKASRKYVK SMQSLKQVID PANRHQQLFL DLCTRLLEHD PDVRIKVQDA LRHPYLT
Validated NT Sequence
atataccatg gctcaccacc accaccacca tatgtctgaa ggtcattaca ttgtcaagcc tgacgatgtt atcggcggta gatacaaaat tgtcaggtta ctgggtcagg gaacgtttgg caaggtggtt gaagccagac atattgagac acggcgtaaa gtcgcgatca aggttatccg agccgtgcaa aagtatcgtg aggcgagcaa aattgagatt agagtgcttg agacgctcag aaagcatgac ccacggaatg ataacaaatg tatacatctc gacgaatact ttgacttccg gaaccacccg tgcctcgtct ccgagttgta cgggatgagt gtatttgact ttttaaagca aaacggtttc caaccattcc cggataagca cattcaagac tttgcaaaga gtttattaag aagcgtgtcc tacttgcatt ctctcaagct cgtacacacc gacctcaagc cggagaatat tcttctatgt tcaaacgagg cgagattagc aggccccaga gttcgaaatg ctcgttcaaa atctatcctc aagaacactg aaatccgtct tatcgatttt ggttctgcca cttttgaatc tgaatatcat tcctctgtcg tctctactag acattatcgt gcccctgaaa tcatacttgg tctttcatgg tcttatcctt gtgacatgtt cagtataggc tgtatcctgg tagagttcta cattggaaac gcgctttttc agacacacga caatttggaa catttggcga tgatggaggt tgtgatgggt aagtttagcc agaggatgat tgagaaggga aaaagtaaga agccagagta tttcaaaggc aacaagatag attttcccaa ctctacggtt tccaaggcga gcagaaaata tgtgaagagc atgcaaagcc tcaagcaagt aatcgatcct gccaacagac atcaacagtt gttccttgac ctttgtacaa gattgctaga gcatgatccg gatgttcgga tcaaggtgca ggacgcttta cgacatccgt atttgaccg
Expected Protein Sequence
MAHHHHHHSE GHYIVKPDDV IGGRYKIVRL LGQGTFGKVV EARHIETRRK VAIKVIRAVQ KYREASKIEI RVLETLRKHD PRNDNKCIHL DEYFDFRNHP CLVSELYGMS VFDFLKQNGF QPFPDKHIQD FAKSLLRSVS YLHSLKLVHT DLKPENILLC SNEARLAGPR VRNARSKSIL KNTEIRLIDF GSATFESEYH SSVVSTRHYR APEIILGLSW SYPCDMFSIG CILVEFYIGN ALFQTHDNLE HLAMMEVVMG KFSQRMIEKG KSKKPEYFKG NKIDFPNSTV SKASRKYVKS MQSLKQVIDP ANRHQQLFLD LCTRLLEHDP DVRIKVQDAL RHPYLTEPIP EPA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctgaag gtcattacat tgtcaagcct gacgatgtta tcggcggtag atacaaaatt gtcaggttac tgggtcaggg aacgtttggc aaggtggttg aagccagaca tattgagaca cggcgtaaag tcgcgatcaa ggttatccga gccgtgcaaa agtatcgtga ggcgagcaaa attgagatta gagtgcttga gacgctcaga aagcatgacc cacggaatga taacaaatgt atacatctcg acgaatactt tgacttccgg aaccacccgt gcctcgtctc cgagttgtac gggatgagtg tatttgactt tttaaagcaa aacggtttcc aaccattccc ggataagcac attcaagact ttgcaaagag tttattaaga agcgtgtcct acttgcattc tctcaagctc gtacacaccg acctcaagcc ggagaatatt cttctatgtt caaacgaggc gagattagca ggccccagag ttcgaaatgc tcgttcaaaa tctatcctca agaacactga aatccgtctt atcgattttg gttctgccac ttttgaatct gaatatcatt cctctgtcgt ctctactaga cattatcgtg cccctgaaat catacttggt ctttcatggt cttatccttg tgacatgttc agtataggct gtatcctggt agagttctac attggaaacg cgctttttca gacacacgac aatttggaac atttggcgat gatggaggtt gtgatgggta agtttagcca gaggatgatt gagaagggaa aaagtaagaa gccagagtat ttcaaaggca acaagataga ttttcccaac tctacggttt ccaaggcgag cagaaaatat gtgaagagca tgcaaagcct caagcaagta atcgatcctg ccaacagaca tcaacagttg ttccttgacc tttgtacaag attgctagag catgatccgg atgttcggat caaggtgcag gacgctttac gacatccgta tttgaccgaa cctattccag agcccgcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat