SSGCID: Target Page

CrneC.10317.b

CMGC/CDK protein kinase

CENTER ID:	CrneC.10317.b
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.10317.b.B2.GE41401	Truncation 1		70	268
CrneC.10317.b.B3.GE41402	Truncation 2		200	476

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_03137
UniProt:	J9VQ29

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRRRLVIGQ GRHGAVIAQP APSDPAIWDR RSRPNASDTS QWGATPLGGL EGWQAVKIVS APPDGRLRSM LPHDVGEEVK ILKRLDHSNI VKLVDYHYET DLLEHYLHFP LFACTLTELF KDPSFPFEPP AIPLPQPSPL ASFNVTTGLS YQLLTALGYL ASSDVAHRDI NPSNLLIDFC GNLKLVDFGT AWSGSSAVMM DNQGETVVRH DRSANGREQS QGDGEGVRAR SSCDVGTGAY RAPELLFSPV KYNPYAVDSW AAGCVIAQMF RPFGSFDPHE FDDSGSDSDS DIRSDSEEGS DGWSDNTEDP DPLDEPWRRV RNSGGDGNGT GSRTGTTTGG DKGGMGERQP MFDASFGTLG LAASIFKIRG TPSLDTWPTF IQLPDAGKIG FPSSHPIPLT SPLILPHLQE GHDELMAAAS KVIEGLLTLD PGRRLTAKKA LESRWFEGVD NVLVQGVCRP WVDVGKKSLE RKIQSV

NT Sequence

atggacagaa gacgcctagt cattgggcaa ggtcgacatg gagccgtgat agcgcaacca gcgccgtcag atcctgcaat ctgggataga aggagtagac caaacgcttc ggacaccagt caatggggcg caacgcctct aggcggtctt gagggatggc aagccgttaa gattgtctct gctcccccag atgggagatt gagaagtatg ctacctcatg atgtagggga agaagttaaa atcctcaaaa gattggatca ctcgaatatc gtcaagcttg tagattacca ctatgaaacg gacctccttg agcactacct tcactttcca ttattcgcct gtaccttgac cgaactgttc aaagatcctt cctttccctt cgagccgcct gctattcctc tacctcaacc ttcccctctg gcgtctttca acgtcaccac aggtttatcg tatcaactgc tcacagccct cggttatctg gcatccagtg acgttgccca ccgcgacatt aatccgtcaa atctcctgat cgacttctgc gggaacctca agcttgtcga tttcggtact gcttggtcgg gttcatctgc cgtgatgatg gacaaccaag gcgagactgt tgtgcggcat gatcgttcag caaatgggag agaacaaagc cagggcgacg gtgaaggtgt cagggctcgg agctcatgtg atgtcggaac gggtgcctac cgggcaccgg aacttctgtt ttcaccagtg aaatacaatc catatgctgt agactcttgg gcagcgggtt gcgtgattgc ccaaatgttt agaccatttg ggtcatttga tcctcacgag tttgatgata gtgggagcga tagcgatagc gacattagaa gtgacagtga agaaggaagt gacggttgga gcgacaatac ggaagatcca gatccacttg atgagccatg gcgtcgcgta cgaaatagcg gtggcgatgg gaatggcact ggttctcgca ctggtactac aactggtggt gataaagggg ggatgggtga aaggcagcca atgttcgacg ctagttttgg taccttgggt ctagcggcta gtatcttcaa aatcagggga actccatcgc ttgatacttg gcctaccttt attcagctcc cagacgcagg caaaattgga ttcccatcct cccatcctat ccctttgacc tctccactta ttcttcctca tcttcaagaa ggacatgatg aattgatggc agcagcaagt aaagtgatag agggtctctt gacccttgac cccggtcgga ggcttaccgc caagaaagct ctagagtcga gatggtttga aggggtggat aacgtacttg tgcaaggagt ttgtcggcca tgggttgacg tagggaagaa gagtttggaa cgcaagattc agagtgttta g

Details for CrneC.10317.b.B2.GE41401

HARVESTED ON:	4/5/2017
SEQUENCED ON:	4/27/2017
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML PHDVGEEVKI LKRLDHSNIV KLVDYHYETD LLEHYLHFPL FACTLTELFK DPSFPFEPPA IPLPQPSPLA SFNVTTGLSY QLLTALGYLA SSDVAHRDIN PSNLLIDFCG NLKLVDFGTA WSGSSAVMMD NQGETVVRHD RSANGREQSQ GDGEGVRARS SCDVGTGAYR APELLFSPVK YNPYAVDSWA AGCVIAQ

Validated NT Sequence

atggctcacc accaccacca ccatatgcta cctcatgatg taggggaaga agttaaaatc ctcaaaagat tggatcactc gaatatcgtc aagcttgtag attaccacta tgaaacggac ctccttgagc actaccttca ctttccatta ttcgcctgta ccttgaccga actgttcaaa gatccttcct ttcccttcga gccgcctgct attcctctac ctcaaccttc ccctctggcg tctttcaacg tcaccacagg tttatcgtat caactgctca cagccctcgg ttatctggca tccagtgacg ttgcccaccg cgacattaat ccgtcaaatc tcctgatcga cttctgcggg aacctcaagc ttgtcgattt cggtactgct tggtcgggtt catctgccgt gatgatggac aaccaaggcg agactgttgt gcggcatgat cgttcagcaa atgggagaga acaaagccag ggcgacggtg aaggtgtcag ggctcggagc tcatgtgatg tcggaacggg tgcctaccgg gcaccggaac ttctgttttc accagtgaaa tacaatccat atgctgtaga ctcttgggca gcgggttgcg tgattgccca ataagtgagt aagat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctac ctcatgatgt aggggaagaa gttaaaatcc tcaaaagatt ggatcactcg aatatcgtca agcttgtaga ttaccactat gaaacggacc tccttgagca ctaccttcac tttccattat tcgcctgtac cttgaccgaa ctgttcaaag atccttcctt tcccttcgag ccgcctgcta ttcctctacc tcaaccttcc cctctggcgt ctttcaacgt caccacaggt ttatcgtatc aactgctcac agccctcggt tatctggcat ccagtgacgt tgcccaccgc gacattaatc cgtcaaatct cctgatcgac ttctgcggga acctcaagct tgtcgatttc ggtactgctt ggtcgggttc atctgccgtg atgatggaca accaaggcga gactgttgtg cggcatgatc gttcagcaaa tgggagagaa caaagccagg gcgacggtga aggtgtcagg gctcggagct catgtgatgt cggaacgggt gcctaccggg caccggaact tctgttttca ccagtgaaat acaatccata tgctgtagac tcttgggcag cgggttgcgt gattgcccaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for CrneC.10317.b.B3.GE41402

HARVESTED ON:	4/5/2017
SEQUENCED ON:	4/27/2017
EXPECTED MW:	31kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD NQGETVVRHD RSANGREQSQ GDGEGVRARS SCDVGTGAYR APELLFSPVK YNPYAVDSWA AGCVIAQMFR PFGSFDPHEF DDSGSDSDSD IRSDSEEGSD GWSDNTEDPD PLDEPWRRVR NSGGDGNGTG SRTGTTTGGD KGGMGERQPM FDASFGTLGL AASIFKIRGT PSLDTWPTFI QLPDAGKIGF PSSHPIPLTS PLILPHLQEG HDELMAAASK VIEGLLTLDP GRRLTAKKAL ESRWFEGVDN VLVQGVCRPW VDVGKKXXXX XIQSV

Validated NT Sequence

tataccatgg ctcaccacca ccaccaccat atggacaacc aaggcgagac tgttgtgcgg catgatcgtt cagcaaatgg gagagaacaa agccagggcg acggtgaagg tgtcagggct cggagctcat gtgatgtcgg aacgggtgcc taccgggcac cggaacttct gttttcacca gtgaaataca atccatatgc tgtagactct tgggcagcgg gttgcgtgat tgcccaaatg tttagaccat ttgggtcatt tgatcctcac gagtttgatg atagtgggag cgatagcgat agcgacatta gaagtgacag tgaagaagga agtgacggtt ggagcgacaa tacggaagat ccagatccac ttgatgagcc atggcgtcgc gtacgaaata gcggtggcga tgggaatggc actggttctc gcactggtac tacaactggt ggtgataaag gggggatggg tgaaaggcag ccaatgttcg acgctagttt tggtaccttg ggtctagcgg ctagtatctt caaaatcagg ggaactccat cgcttgatac ttggcctacc tttattcagc tcccagacgc aggcaaaatt ggattcccat cctcccatcc tatccctttg acctctccac ttattcttcc tcatcttcaa gaaggacatg atgaattgat ggcagcagca agtaaagtga tagagggtct cttgaccctt gaccccggtc ggaggcttac cgccaagaaa gctctagagt cgagatggtt tgaaggggtg gataacgtac ttgtgcaagg agtttgtcgg ccatgggttg acgtagggaa gaagannnnn nnnnnnnaga ttcagagtgt ttaagtgagt aaga

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaca accaaggcga gactgttgtg cggcatgatc gttcagcaaa tgggagagaa caaagccagg gcgacggtga aggtgtcagg gctcggagct catgtgatgt cggaacgggt gcctaccggg caccggaact tctgttttca ccagtgaaat acaatccata tgctgtagac tcttgggcag cgggttgcgt gattgcccaa atgtttagac catttgggtc atttgatcct cacgagtttg atgatagtgg gagcgatagc gatagcgaca ttagaagtga cagtgaagaa ggaagtgacg gttggagcga caatacggaa gatccagatc cacttgatga gccatggcgt cgcgtacgaa atagcggtgg cgatgggaat ggcactggtt ctcgcactgg tactacaact ggtggtgata aaggggggat gggtgaaagg cagccaatgt tcgacgctag ttttggtacc ttgggtctag cggctagtat cttcaaaatc aggggaactc catcgcttga tacttggcct acctttattc agctcccaga cgcaggcaaa attggattcc catcctccca tcctatccct ttgacctctc cacttattct tcctcatctt caagaaggac atgatgaatt gatggcagca gcaagtaaag tgatagaggg tctcttgacc cttgaccccg gtcggaggct taccgccaag aaagctctag agtcgagatg gtttgaaggg gtggataacg tacttgtgca aggagtttgt cggccatggg ttgacgtagg gaagaagagt ttggaacgca agattcagag tgtttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat