CrneC.01515.b

AGC protein kinase (AGC protein kinase, variant 1) (AGC protein kinase, variant 2)

CENTER ID: CrneC.01515.b
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.01515.b.B2.GE41394 Truncation 1 74 410
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_03258
UniProt: J9VV70
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDSTPYSIRY RILSDLLDSN IALAYDNAFI VDGESSTCLA EDEKAAVLDT VLLRSWNGET SCLDSVTFNI LERNRLKHDD FDIIGCLGEG QFGVVEAVRF KMSGQVFAMK TIEKTTAKRA GRLLSLSLER HVHRLSSSSS LSPCPKLVAA FQTELSLHLI ITYAECGSLW NRMCALFSDT KYPGCMPEEE IQWWGAQMVN AVGWLHSHNI VHRDIKPHNF LIKSDHHLQI TDFGSAAPLY FTLPDEPLCV PWQFCVQPVG TPDYLAPEVL ILAEQAVIES EQVHQTSDSH IPQHSEKKGY NASIDWWSLG STLYEMATGK PPFFAETIHE TYEKLITFRS DNLSFPTYLS SELISLLKDL INVPVKRLST ATMIQKSPFF QPTGWIDNCP KILCPPLISH DMLDNEQLQT NLQQSIQEDK FTFSHFFDFS NTSSVHLTPV SPSSSAPPVW SQWVGWSFHP DPDSLEVPSA ADNSSGNAFM TPVRNKLTTC PFTPGIMNQP LAPLLPSTRS TKKQAFQELL HCVQMSAKKH MMRYNSQSAH STSPCMTPVA RGQMKTKSFT KDTSCVSLLS DPGMVLVETG ASHQWLQERH DVLCGQLHHL DQQLFKLQKL LKC
NT Sequence
atggactcta ctccttacag tatccgatat cgtatcttat ccgacttact cgacagcaat atagcgctgg cttatgacaa tgccttcatt gttgatggag agtcaagcac ttgccttgcc gaagacgaga aggcggcggt attggacact gtgctgcttc gaagttggaa tggtgaaaca tcgtgtttag attctgtcac tttcaatatc cttgagcgca atcgtctcaa acatgatgat ttcgatatta taggctgttt aggggaaggg caatttgggg tggtagaagc tgtgagattt aagatgagcg gacaagtatt tgctatgaag acaatcgaaa agactacggc taaacgtgct ggacgactgc tgtctttatc gctggaacgc catgttcata ggctctccag ttcatcctct ctgtcaccgt gtccaaaatt ggttgcagcg ttccagacag agctatctct tcatcttatc attacctatg ctgaatgtgg ttctttgtgg aatcggatgt gtgccctctt ctctgacacc aagtaccctg ggtgcatgcc tgaagaagaa attcagtggt ggggagcgca gatggtcaat gctgttgggt ggcttcatag ccataatata gtgcaccgtg acatcaaacc acacaatttt ttaattaaat cagatcatca tctacaaatc accgactttg gctcagcagc ccctctttac ttcactcttc ctgatgaacc actttgtgtc ccctggcaat tctgtgtcca gccagttggg acaccagatt accttgcgcc tgaggtactg atcttagctg aacaagctgt gatcgaatca gaacaagtgc accaaacatc tgatagccat atacctcagc actcagaaaa gaaaggatat aatgccagca ttgattggtg gagtttagga tcaactctgt atgagatggc aacagggaaa cctcctttct ttgctgagac aatccatgaa acatatgaaa aacttatcac tttcagaagt gacaatctgt catttcctac ttatctgtca tcagaactaa tttcacttct aaaagatttg atcaatgtac cagtcaaaag gttgtcaact gctacaatga tacaaaaatc cccatttttc caacctacag gctggattga taattgtccc aaaattttgt gtccaccatt gatatcacat gatatgcttg ataatgagca acttcaaaca aaccttcaac aatcaataca ggaagacaag ttcacattca gccatttctt tgacttctca aacacctctt cagttcacct tactccagtc tcaccatctt cttctgcccc tcctgtttgg tcccagtggg ttggctggtc attccatcct gacccagata gccttgaagt cccttcagca gcagataatt cttctggaaa tgcttttatg accccagtcc gaaataaact tacaacatgc ccattcactc caggcatcat gaaccaacca ctggcaccac tattgccttc tactcgcagc acaaagaaac aagcatttca agagctctta cattgtgtac aaatgagtgc caaaaagcat atgatgaggt ataattcaca atcagcccat tctaccagtc cttgtatgac tcctgttgcc agaggccaga tgaaaacgaa atcattcacc aaagacacat cctgtgtcag tttgttatct gatccgggca tggtattagt tgaaacagga gcttcacatc aatggctgca agaaagacat gatgtcttat gtggacaatt acatcacctt gatcaacagc tgttcaagct gcaaaagtta ttgaagtgct ga
Details for CrneC.01515.b.B2.GE41394
HARVESTED ON: data unavailable
SEQUENCED ON: 4/27/2017
EXPECTED MW: 39kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMX XLKHDDFDII GCLGEGQFGV VEAVRFKMSG QVFAMKTIEK TTAKRAGRLL SLSLERHVHR LSSSSSLSPC PKLVAAFQTE LSLHLIITYA ECGSLWNRMC ALFSDTKYPG CMPEEEIQWW GAQMVNAVGW LHSHNIVHRD IKPHNFLIKS DHHLQITDFG SAAPLYFTLP DEPLCVPWQF CVQPVGTPDY LAPEVLILAE QAVIESEQVH QTSDSHIPQH SEKKGYNASI DWWSLGSTLY EMATGKPPFF AETIHETYEK LITFRSDNLS FPTYLSSELI SLLKDLINVP VKRLSTATMI QKSPFFQPTG WIDNCPKILC PPLISHDMXX XXQ
Validated NT Sequence
atataccatg gctcaccacc accaccacca tatgnnnnnt ctcaaacatg atgatttcga tattataggc tgtttagggg aagggcaatt tggggtggta gaagctgtga gatttaagat gagcggacaa gtatttgcta tgaagacaat cgaaaagact acggctaaac gtgctggacg actgctgtct ttatcgctgg aacgccatgt tcataggctc tccagttcat cctctctgtc accgtgtcca aaattggttg cagcgttcca gacagagcta tctcttcatc ttatcattac ctatgctgaa tgtggttctt tgtggaatcg gatgtgtgcc ctcttctctg acaccaagta ccctgggtgc atgcctgaag aagaaattca gtggtgggga gcgcagatgg tcaatgctgt tgggtggctt catagccata atatagtgca ccgtgacatc aaaccacaca attttttaat taaatcagat catcatctac aaatcaccga ctttggctca gcagcccctc tttacttcac tcttcctgat gaaccacttt gtgtcccctg gcaattctgt gtccagccag ttgggacacc agattacctt gcgcctgagg tactgatctt agctgaacaa gctgtgatcg aatcagaaca agtgcaccaa acatctgata gccatatacc tcagcactca gaaaagaaag gatataatgc cagcattgat tggtggagct taggatcaac tctgtatgag atggcaacag ggaaacctcc tttctttgct gagacaatcc atgaaacata tgaaaaactt atcactttca gaagtgacaa tctgtcattt cctacttatc tgtcatcaga actaatttca cttctaaaag atttgatcaa tgtaccagtc aaaaggttgt caactgctac aatgatacaa aaatccccat ttttccaacc tacaggctgg attgataatt gtcccaaaat tttgtgtcca ccattgatat cacatgatat gnnnnnnnnn nagcaactt
Expected Protein Sequence
MAHHHHHHNR LKHDDFDIIG CLGEGQFGVV EAVRFKMSGQ VFAMKTIEKT TAKRAGRLLS LSLERHVHRL SSSSSLSPCP KLVAAFQTEL SLHLIITYAE CGSLWNRMCA LFSDTKYPGC MPEEEIQWWG AQMVNAVGWL HSHNIVHRDI KPHNFLIKSD HHLQITDFGS AAPLYFTLPD EPLCVPWQFC VQPVGTPDYL APEVLILAEQ AVIESEQVHQ TSDSHIPQHS EKKGYNASID WWSLGSTLYE MATGKPPFFA ETIHETYEKL ITFRSDNLSF PTYLSSELIS LLKDLINVPV KRLSTATMIQ KSPFFQPTGW IDNCPKILCP PLISHDMLDN EQLQT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatcgtc tcaaacatga tgatttcgat attataggct gtttagggga agggcaattt ggggtggtag aagctgtgag atttaagatg agcggacaag tatttgctat gaagacaatc gaaaagacta cggctaaacg tgctggacga ctgctgtctt tatcgctgga acgccatgtt cataggctct ccagttcatc ctctctgtca ccgtgtccaa aattggttgc agcgttccag acagagctat ctcttcatct tatcattacc tatgctgaat gtggttcttt gtggaatcgg atgtgtgccc tcttctctga caccaagtac cctgggtgca tgcctgaaga agaaattcag tggtggggag cgcagatggt caatgctgtt gggtggcttc atagccataa tatagtgcac cgtgacatca aaccacacaa ttttttaatt aaatcagatc atcatctaca aatcaccgac tttggctcag cagcccctct ttacttcact cttcctgatg aaccactttg tgtcccctgg caattctgtg tccagccagt tgggacacca gattaccttg cgcctgaggt actgatctta gctgaacaag ctgtgatcga atcagaacaa gtgcaccaaa catctgatag ccatatacct cagcactcag aaaagaaagg atataatgcc agcattgatt ggtggagttt aggatcaact ctgtatgaga tggcaacagg gaaacctcct ttctttgctg agacaatcca tgaaacatat gaaaaactta tcactttcag aagtgacaat ctgtcatttc ctacttatct gtcatcagaa ctaatttcac ttctaaaaga tttgatcaat gtaccagtca aaaggttgtc aactgctaca atgatacaaa aatccccatt tttccaacct acaggctgga ttgataattg tcccaaaatt ttgtgtccac cattgatatc acatgatatg cttgataatg agcaacttca aacatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat