CrneC.01515.a

AGC/YANK protein kinase

CENTER ID: CrneC.01515.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.01515.a.B3.GE41338 Truncation 2 1 363
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_03811
UniProt: J9VG11

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGASCCKPEA IDFEGEVNLF HFYLLRSVGK GAFGKVRVVQ HKHTKTLFAL KYINKQKCVK MKAVANIVQE RRLLEEIDHP FVVNLRYAFQ DDENCFFVLD LMLGGDLRFH LDRAGAMSEE IVRFYVAEIA LAIDYLHSKR IVHRDLKPDN ILLDEKGHAH ITDFNIAVHF SDRRLLTGVA GSMAYMAPEI LTKRGYSAPV DFWSLGILAY ELLFGKRPFR GRTNSALTNS ILNEALSWPE DAPGRCSSDG MHAIRGFLER DPNKRLGYRP GGGGMDNIKN HPWFRNINWE QLYDKDVVPP FEPDSKRANF DATHELEELL LEENPLKARK RKEGQDIALL SPEMRMMEQH FKVFDYTKAQ RRSYYIPNPS QGSILSATAA ISSPNATRPS PQIDITRPDT PSDQTGVISK TGMDVEAQIL DGGGMANMGG RGGWRSSDLV ERGHTVSDYR PTSTKLLTPS QHSSIEPSPT LEEHMIERHG ENAL
NT Sequence
atgggtgcta gctgttgtaa accagaggcc atcgactttg aaggtgaagt caacctcttc cacttttatc tgctacggag tgttggaaag ggagcttttg gaaaggttcg tgttgtccaa cataagcata caaagacttt gtttgccctc aagtacatca ataaacaaaa atgtgtcaag atgaaggcag tagccaacat cgtccaagag cggcggcttc ttgaagagat tgaccatccg ttcgtcgtaa atctgcggta tgcgtttcaa gatgatgaga actgtttctt tgtccttgat cttatgttgg gaggggattt gcgcttccat cttgatcgtg ctggtgctat gagtgaagag attgtgcgat tctatgtggc cgagatcgct ttggccattg attatttaca ctcaaagcgg attgtgcata gggatttaaa accagataac attctattag atgagaaggg ccatgctcat ataactgact tcaatatcgc tgtccacttt tcagatcgaa gacttctgac aggagtggca ggaagcatgg catatatggc tccggagatc ttgacaaaac gcggatattc tgccccggta gatttttggt cattagggat acttgcgtat gagcttctat tcggcaaacg tcctttccgg ggccgaacaa atagtgcttt gaccaactcg atcctcaacg aagctctcag ttggcccgaa gacgcccccg gcagatgttc ttctgatgga atgcacgcta ttcgtggttt tttagaacga gatccaaata aacgactagg atataggccg ggcggaggtg gtatggacaa tataaaaaat catccttggt tccggaatat taattgggaa cagctgtatg ataaagatgt tgtgccacca tttgaaccag attcgaagcg tgccaatttt gacgccacac atgagctgga agagctgttg ttggaggaga atccgctcaa ggcgaggaaa cgaaaagaag gtcaagacat tgcgttattg tcgccagaga tgcgtatgat ggagcagcat ttcaaagtat ttgactacac gaaagcgcag cgacgctcat attatatccc aaatccctca caaggcagta tcctttctgc tacagctgcc atatcctcac caaacgccac ccgtccatca cctcagatcg acataacgcg tccagatact cccagtgacc aaaccggcgt gatcagcaaa accggaatgg atgtcgaagc tcagatactg gatgggggag gaatggcgaa catggggggc agaggcggtt ggagatcatc agatttagta gaacgggggc atacagtttc ggattatcga cccacatcaa caaagctact gaccccttca cagcactctt caatcgaacc gtctccgact ctagaagaac atatgattga gaggcacgga gagaacgccc tttga
Details for CrneC.01515.a.B3.GE41338
HARVESTED ON: 4/3/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG ASCCKPEAID FEGEVNLFHF YLLRSVGKGA FGKVRVVQHK HTKTLFALKY INKQKCVKMK AVANIVQERR LLEEIDHPFV VNLRYAFQDD ENCFFVLDLM LGGDLRFHLD RAGAMSEEIV RFYVAEIALA IDYLHSKRIV HRDLKPDNIL LDEKGYAHIT DFNIAVHFSD RRLLTGVAGS MAYMAPEILT KRGYSAPVDF WSLGILAYEL LFGKRPFRGR TNSALTNSIL NEALSWPEDA PGRCSSDGMH AIRGFLERDP NKRLGYRPGG GGMDNIKNHP WFRNINWEQL YDKDVVPPFE PDSKRANFDA THELEELLLE ENPLKARKRK EGQDIALLSP EMRMMEQHFK VFD
Validated NT Sequence
gtagtcaaat actttgaaat gctgctccat catacgcatc tctggcgaca ataacgcaat gtcttgacct tcttttcgtt tcctcgcctt gagcggattc tcctccaaca acagctcttc cagctcatgt gtggcgtcaa aattggcacg cttcgaatct ggttcaaatg gtggcacaac atctttatca tacagctgtt cccaattaat attccggaac caaggatgat tttttatatt gtccatacca cctccgcccg gcctatatcc tagtcgttta tttggatctc gttctaaaaa accacgaata gcgtgcattc catcagaaga acatctgccg ggggcgtctt cgggccaact gagagcttcg ttgaggatcg agttggtcaa agcactattt gttcggcccc ggaaaggacg tttgccgaat agaagctcat acgcaagtat ccctaatgac caaaaatcta ccggggcaga atatccgcgt tttgtcaaga tctccggagc catatatgcc atgcttcctg ccactcctgt cagaagtctt cgatctgaaa agtggacagc gatattgaag tcagttatat gagcatagcc cttctcatct aatagaatgt tatctggttt taaatcccta tgcacaatcc gctttgagtg taaataatca atggccaaag cgatctcggc cacatagaat cgcacaatct cttcactcat agcaccagca cgatcaagat ggaagcgcaa atcccctccc aacataagat caaggacaaa gaaacagttc tcatcatctt gaaacgcata ccgcagattt acgacgaacg gatggtcaat ctcttcaaga agccgccgct cttggacgat gttggctact gccttcatct tgacacattt ttgtttattg atgtacttga gggcaaacaa agtctttgta tgcttatgtt ggacaacacg aacctttcca aaagctccct ttccaacact ccgtagcaga taaaagtgga agaggttgac ttcaccttca aagtcgatgg cctctggttt acaacagcta gcacccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMG ASCCKPEAID FEGEVNLFHF YLLRSVGKGA FGKVRVVQHK HTKTLFALKY INKQKCVKMK AVANIVQERR LLEEIDHPFV VNLRYAFQDD ENCFFVLDLM LGGDLRFHLD RAGAMSEEIV RFYVAEIALA IDYLHSKRIV HRDLKPDNIL LDEKGHAHIT DFNIAVHFSD RRLLTGVAGS MAYMAPEILT KRGYSAPVDF WSLGILAYEL LFGKRPFRGR TNSALTNSIL NEALSWPEDA PGRCSSDGMH AIRGFLERDP NKRLGYRPGG GGMDNIKNHP WFRNINWEQL YDKDVVPPFE PDSKRANFDA THELEELLLE ENPLKARKRK EGQDIALLSP EMRMMEQHFK VFDYTKAQRR S
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggtg ctagctgttg taaaccagag gccatcgact ttgaaggtga agtcaacctc ttccactttt atctgctacg gagtgttgga aagggagctt ttggaaaggt tcgtgttgtc caacataagc atacaaagac tttgtttgcc ctcaagtaca tcaataaaca aaaatgtgtc aagatgaagg cagtagccaa catcgtccaa gagcggcggc ttcttgaaga gattgaccat ccgttcgtcg taaatctgcg gtatgcgttt caagatgatg agaactgttt ctttgtcctt gatcttatgt tgggagggga tttgcgcttc catcttgatc gtgctggtgc tatgagtgaa gagattgtgc gattctatgt ggccgagatc gctttggcca ttgattattt acactcaaag cggattgtgc atagggattt aaaaccagat aacattctat tagatgagaa gggccatgct catataactg acttcaatat cgctgtccac ttttcagatc gaagacttct gacaggagtg gcaggaagca tggcatatat ggctccggag atcttgacaa aacgcggata ttctgccccg gtagattttt ggtcattagg gatacttgcg tatgagcttc tattcggcaa acgtcctttc cggggccgaa caaatagtgc tttgaccaac tcgatcctca acgaagctct cagttggccc gaagacgccc ccggcagatg ttcttctgat ggaatgcacg ctattcgtgg ttttttagaa cgagatccaa ataaacgact aggatatagg ccgggcggag gtggtatgga caatataaaa aatcatcctt ggttccggaa tattaattgg gaacagctgt atgataaaga tgttgtgcca ccatttgaac cagattcgaa gcgtgccaat tttgacgcca cacatgagct ggaagagctg ttgttggagg agaatccgct caaggcgagg aaacgaaaag aaggtcaaga cattgcgtta ttgtcgccag agatgcgtat gatggagcag catttcaaag tatttgacta cacgaaagcg cagcgacgct catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat