CrneC.01505.a

CK1/CK1/CK1-G protein kinase, variant

CENTER ID: CrneC.01505.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.01505.a.B2.GE41393 Truncation 1 24 323
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_00556
UniProt: T2BQF0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATTHVIGSS NPGGHLTSSG NHSSSSSNIV GVHYKVGKKI GEGSFGVIFE GTNLLNSQTV AIKFEPRKSD APQLRDEYRS YKILSGCLGI PQVYYFGQEG LHNILVIDLL GPSLEDLFDM CGRKFTVKTC CMTAKQMLSR VQTIHEKNLI YRDIKPDNFL IGRPSSKNAN LIHVVDFGMA KQYRDPKTKQ HIPYRERKSL SGTARYMSIN THLGREQSRR DDLEALGHVF FYFLRGGLPW QGLKAATNKQ KYEKIGEKKQ TTPIAELVEG YPNEFSIYLN YVRKLTFDET PDYDFLRGLF DLALSNCGEM DDGVYDWMML NNGQGWEASG RQSAAQAEIT RHNTRGRDRE YRDRVDKLRN GSAANQPSPS KPRKSGSALP NVSNQAMVGV SAPSPLPQSR RQSQQAVQSQ AGLQPISPMN VNSRGGNTAA NSQQLGEGDY HQENKGNGFI RFITCGCFR
NT Sequence
atggctacca cacacgtcat cggctcttcc aaccccggcg gccatctcac ctcctctgga aaccactctt ccagctcgag caacatcgtc ggcgtccact acaaggttgg caagaagatt ggagaaggct cttttggcgt catctttgag ggcacaaatc tgctcaattc acagacagtc gccatcaagt ttgaacctcg gaaatcagat gcccctcaac tccgcgatga gtatagaagt tacaagatct tgagtgggtg cctcggtatc cctcaggtgt actactttgg tcaggaaggt ttgcataaca tccttgtgat cgacttgctg ggcccgtctc ttgaagactt atttgacatg tgcggaagaa aatttactgt caaaacgtgc tgtatgactg ctaagcagat gctctcacgt gtacagacca tccacgaaaa aaacctaatc tatcgtgata tcaaacccga caactttttg attggacgac cgagtagcaa gaacgccaat ctcattcacg tagttgactt tggtatggca aagcaataca gagacccaaa gacgaagcaa cacattccct atagagaaag aaagagcctg agcggaactg cccgttacat gagtatcaat acccacctgg gtcgagagca atcccgacga gatgatctcg aagccctcgg tcatgtcttc ttctactttc ttcgtggagg tcttccatgg caaggcctca aggcggccac caacaagcaa aagtacgaga aaattggaga aaagaagcag acaactccca ttgctgagct tgtggaaggt tatcccaacg aattctctat ctatctcaat tatgttcgca aattgacatt tgatgagacg cccgactatg actttttacg aggtttattt gacttggcat tgtccaactg cggagagatg gacgatggtg tttatgattg gatgatgttg aacaatggtc aagggtggga ggcttcgggg cgtcagtctg ctgcgcaggc cgagatcact cgacacaata cacgcggtcg agacagggag tatcgagaca gggttgacaa gctcaggaac ggatccgctg ctaatcagcc ttccccgagc aaaccaagga aatctggctc agctttgcca aatgtcagca accaggccat ggtcggtgtc tcggcgccta gcccgttacc tcaatctcga cgacagtcgc agcaagctgt gcaatctcaa gcaggtctgc aacccatcag cccgatgaac gtcaattctc gaggaggcaa cacagccgcc aactctcagc agcttggaga gggggattac caccaggaga acaagggaaa cggtttcatc aggtttataa cttgcggctg cttccgatag
Details for CrneC.01505.a.B2.GE41393
HARVESTED ON: 4/5/2017
SEQUENCED ON: 4/27/2017
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS SSSNIVGVHY KVGKKIGEGS FGVIFEGTNL LNSQTVAIKF EPRKSDAPQL RDEYRSYKIL SGCLGIPQVY YFGQEGLHNI LVIDLLGPSL EDLFDMCGRK FTVKTCCMTA KQMLSRVQTI HEKNLIYRDI KPDNFLIGRP SSKNANLIHV VDFGMAKQYR DPKTKQHIPY RERKSLSGTA RYMSINTHLG REQSRRDDLE ALGHVFFYFL RGGLPWQGLK AATNKQKYEK IGEKKQTTPI AELVEGYPNE FSIYLNYVRK LTFDETPDYD FLRGLFDLAL SNCGEMDDGV YXXXMLNNG
Validated NT Sequence
agatatacca tggctcacca ccaccaccac catatgtcca gctcgagcaa catcgtcggc gtccactaca aggttggcaa gaagattgga gaaggctctt ttggcgtcat ctttgagggc acaaatctgc tcaattcaca gacagtcgcc atcaagtttg aacctcggaa atcagatgcc cctcaactcc gcgatgagta tagaagttac aagatcttga gtgggtgcct cggtatccct caggtgtact actttggtca ggaaggtttg cataacatcc ttgtgatcga cttgctgggc ccgtctcttg aagacttatt tgacatgtgc ggaagaaaat ttactgtcaa aacgtgctgt atgactgcta agcagatgct ctcacgtgta cagaccatcc acgaaaaaaa cctaatctat cgtgatatca aacccgacaa ctttttgatt ggacgaccga gtagcaagaa cgccaatctc attcacgtag ttgactttgg tatggcaaag caatacagag acccaaagac gaagcaacac attccctata gagaaagaaa gagcctgagc ggaactgccc gttacatgag tatcaatacc cacctgggtc gagagcaatc ccgacgagat gatctcgaag ccctcggtca tgtcttcttc tactttcttc gtggaggtct tccatggcaa ggcctcaagg cggccaccaa caagcaaaag tacgagaaaa ttggagaaaa gaagcagaca actcccattg ctgagcttgt ggaaggttat cccaacgaat tctctatcta tctcaattat gttcgcaaat tgacatttga tgagacgccc gactatgact ttttacgagg tttatttgac ttggcattgt ccaactgcgg agagatggac gatggtgttt atnnnnnnnn natgttgaac aatggttaag tgagtaa
Expected Protein Sequence
MAHHHHHHSS SSNIVGVHYK VGKKIGEGSF GVIFEGTNLL NSQTVAIKFE PRKSDAPQLR DEYRSYKILS GCLGIPQVYY FGQEGLHNIL VIDLLGPSLE DLFDMCGRKF TVKTCCMTAK QMLSRVQTIH EKNLIYRDIK PDNFLIGRPS SKNANLIHVV DFGMAKQYRD PKTKQHIPYR ERKSLSGTAR YMSINTHLGR EQSRRDDLEA LGHVFFYFLR GGLPWQGLKA ATNKQKYEKI GEKKQTTPIA ELVEGYPNEF SIYLNYVRKL TFDETPDYDF LRGLFDLALS NCGEMDDGVY DWMMLNNG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccagct cgagcaacat cgtcggcgtc cactacaagg ttggcaagaa gattggagaa ggctcttttg gcgtcatctt tgagggcaca aatctgctca attcacagac agtcgccatc aagtttgaac ctcggaaatc agatgcccct caactccgcg atgagtatag aagttacaag atcttgagtg ggtgcctcgg tatccctcag gtgtactact ttggtcagga aggtttgcat aacatccttg tgatcgactt gctgggcccg tctcttgaag acttatttga catgtgcgga agaaaattta ctgtcaaaac gtgctgtatg actgctaagc agatgctctc acgtgtacag accatccacg aaaaaaacct aatctatcgt gatatcaaac ccgacaactt tttgattgga cgaccgagta gcaagaacgc caatctcatt cacgtagttg actttggtat ggcaaagcaa tacagagacc caaagacgaa gcaacacatt ccctatagag aaagaaagag cctgagcgga actgcccgtt acatgagtat caatacccac ctgggtcgag agcaatcccg acgagatgat ctcgaagccc tcggtcatgt cttcttctac tttcttcgtg gaggtcttcc atggcaaggc ctcaaggcgg ccaccaacaa gcaaaagtac gagaaaattg gagaaaagaa gcagacaact cccattgctg agcttgtgga aggttatccc aacgaattct ctatctatct caattatgtt cgcaaattga catttgatga gacgcccgac tatgactttt tacgaggttt atttgacttg gcattgtcca actgcggaga gatggacgat ggtgtttatg attggatgat gttgaacaat ggttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat