SSGCID: Target Page

CrneC.01402.a

Fructose-bisphosphate aldolase 1 (FBA1) (CNAG_06770)

CENTER ID:	CrneC.01402.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.01402.a.B1.GE42736	full length		1	359

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.01402.a.B1.PW38591	full length		1	359

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_06770
UniProt:	J9VI18

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSGALSVVPA GVITGDNVRK LFKYAKDNKF AIPAINVTSS SVVNAVLEAA RDINSPIILQ VSQGGAAFFA GKGLKNDNQE ASIAGAVAAA HFIRSVAPAY GVPVVLHSDH CAKKLLPWFD GMLEADEAYF KEHGEPLFSS HMLDLSEESK EDNIKDCVFY FKRMAKVNLW LEMEIGITGG EEDGVDNTSV DNNSLYTQPE DIWDVYSALN AISPNFSIAA GFGNVHGVYK PGNVKLRPEL LGKHQKYTHE QIGGDEEKPL YLVFHGGSGS TKDEIREAVV NGVVKMNVDT DTQWAYLCGV RDFILKKKDY LMTQVGNPDG ADKPNKKQYD PRVWVREGEK TLVERVKEAC VDLGNKNRN

NT Sequence

atgtctggtg ctctctctgt cgtccccgct ggtgttatca ctggcgacaa cgttcgaaag ctcttcaagt atgctaagga caacaagttc gctatccctg ccatcaacgt cacttcctct tccgttgtca acgctgtcct tgaggctgcc cgggacatca actcccctat catcctccag gtttcccagg gtggtgccgc tttcttcgcc ggtaagggct tgaagaacga caaccaggag gcttccatcg ctggtgccgt cgctgctgcc cacttcatcc gttccgttgc tcccgcctac ggcgttcccg tcgtcctcca ctctgaccac tgtgccaaga agctccttcc ttggtttgat ggtatgctcg aggccgacga ggcttacttc aaggagcacg gagagcccct tttctcctcc cacatgcttg acttgtctga ggagtccaag gaggacaaca tcaaggactg tgtcttctac ttcaagcgaa tggccaaggt taacctctgg cttgaaatgg aaatcggtat caccggtggt gaggaggacg gtgttgacaa cacctctgtc gacaacaact ctctctacac ccagcccgag gacatctggg atgtctactc tgcccttaac gccatctctc ccaacttctc tatcgctgcc ggttttggta acgtccacgg tgtttacaag cccggaaacg tcaagctcag gcctgagctc ctcggcaagc accagaagta cacccacgag cagatcggcg gtgacgagga gaagcccttg taccttgtct tccacggtgg ttccggttct accaaggatg agatccgaga ggctgttgtc aacggcgttg ttaagatgaa cgttgacact gacacccaat gggcttacct ctgcggtgtc cgtgacttca tcctcaagaa gaaggactac ctcatgactc aggttggtaa ccccgatggt gccgacaagc ccaacaagaa gcagtacgac ccccgagtct gggttaggga gggtgagaag actctcgtcg agcgagtcaa ggaagcctgt gtcgacttgg gcaacaaaaa caggaactaa

Details for CrneC.01402.a.B1.GE42736

HARVESTED ON:	11/5/2018
SEQUENCED ON:	11/7/2018
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS GALSVVPAGV ITGDNVRKLF KYAKDNKFAI PAINVTSSSV VNAVLEAARD INSPIILQVS QGGAAFFAGK GLKNDNQEAS IAGAVAAAHF IRSVAPAYGV PVVLHSDHCA KKLLPWFDGM LEADEAYFKE HGEPLFSSHM LDLSEESKED NIKDCVFYFK RMAKVNLWLE MEIGITGGEE DGVDNTSVDN NSLYTQPEDI WDVYSALNAI SPNFSIAAGF GNVHGVYKPG NVKLRPELLG KHQKYTHEQI GGDEEKPLYL VFHGGSGSTK DEIREAVVNG VVKMNVDTDT QWAYLCGVRD FILKKKDYLM TQVGNPDGAD KPNKKQYDPR VWVREGEKTL VERVKEAC

Validated NT Sequence

acacaggctt ccttgactcg ctcgacgaga gtcttctcac cctccctaac ccagactcgg gggtcgtact gcttcttgtt gggcttgtcg gcaccatcgg ggttaccaac ctgagtcatg aggtagtcct tcttcttgag gatgaagtca cggacaccgc agaggtaagc ccattgggtg tcagtgtcaa cgttcatctt aacaacgccg ttgacaacag cctctcggat ctcatccttg gtagaaccgg aaccaccgtg gaagacaagg tacaagggct tctcctcgtc accgccgatc tgctcgtggg tgtacttctg gtgcttgccg aggagctcag gcctgagctt gacgtttccg ggcttgtaaa caccgtggac gttaccaaaa ccggcagcga tagagaagtt gggagagatg gcgttaaggg cagagtagac atcccagatg tcctcgggct gggtgtagag agagttgttg tcgacagagg tgttgtcaac accgtcctcc tcaccaccgg tgataccgat ttccatttca agccagaggt taaccttggc cattcgcttg aagtagaaga cacagtcctt gatgttgtcc tccttggact cctcagacaa gtcaagcatg tgggaggaga aaaggggctc tccgtgctcc ttgaagtaag cctcgtcggc ctcgagcata ccatcaaacc aaggaaggag cttcttggca cagtggtcag agtggaggac gacgggaacg ccgtaggcgg gagcaacgga acggatgaag tgggcagcag cgacggcacc agcgatggaa gcctcctggt tgtcgttctt caagccctta ccggcgaaga aagcggcacc accctgggaa acctggagga tgatagggga gttgatgtcc cgggcagcct caaggacagc gttgacaacg gaagaggaag tgacgttgat ggcagggata gcgaacttgt tgtccttagc atacttgaag agctttcgaa cgttgtcgcc agtgataaca ccagcgggga cgacagagag agcaccagac atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg gtgctctctc tgtcgtcccc gctggtgtta tcactggcga caacgttcga aagctcttca agtatgctaa ggacaacaag ttcgctatcc ctgccatcaa cgtcacttcc tcttccgttg tcaacgctgt ccttgaggct gcccgggaca tcaactcccc tatcatcctc caggtttccc agggtggtgc cgctttcttc gccggtaagg gcttgaagaa cgacaaccag gaggcttcca tcgctggtgc cgtcgctgct gcccacttca tccgttccgt tgctcccgcc tacggcgttc ccgtcgtcct ccactctgac cactgtgcca agaagctcct tccttggttt gatggtatgc tcgaggccga cgaggcttac ttcaaggagc acggagagcc ccttttctcc tcccacatgc ttgacttgtc tgaggagtcc aaggaggaca acatcaagga ctgtgtcttc tacttcaagc gaatggccaa ggttaacctc tggcttgaaa tggaaatcgg tatcaccggt ggtgaggagg acggtgttga caacacctct gtcgacaaca actctctcta cacccagccc gaggacatct gggatgtcta ctctgccctt aacgccatct ctcccaactt ctctatcgct gccggttttg gtaacgtcca cggtgtttac aagcccggaa acgtcaagct caggcctgag ctcctcggca agcaccagaa gtacacccac gagcagatcg gcggtgacga ggagaagccc ttgtaccttg tcttccacgg tggttccggt tctaccaagg atgagatccg agaggctgtt gtcaacggcg ttgttaagat gaacgttgac actgacaccc aatgggctta cctctgcggt gtccgtgact tcatcctcaa gaagaaggac tacctcatga ctcaggttgg taaccccgat ggtgccgaca agcccaacaa gaagcagtac gacccccgag tctgggttag ggagggtgag aagactctcg tcgagcgagt caaggaagcc tgtgtcgact tgggcaacaa aaacaggaac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for CrneC.01402.a.B1.PW38591

PURIFICATION DATe:	1/22/2019
CONCENTRATION:	47.96mg/ml
OBSERVED MW:	39kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 8, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	20
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTG GTGCTCTCTC TGTCGTCCCC GCTGGTGTTA TCACTGGCGA CAACGTTCGA AAGCTCTTCA AGTATGCTAA GGACAACAAG TTCGCTATCC CTGCCATCAA CGTCACTTCC TCTTCCGTTG TCAACGCTGT CCTTGAGGCT GCCCGGGACA TCAACTCCCC TATCATCCTC CAGGTTTCCC AGGGTGGTGC CGCTTTCTTC GCCGGTAAGG GCTTGAAGAA CGACAACCAG GAGGCTTCCA TCGCTGGTGC CGTCGCTGCT GCCCACTTCA TCCGTTCCGT TGCTCCCGCC TACGGCGTTC CCGTCGTCCT CCACTCTGAC CACTGTGCCA AGAAGCTCCT TCCTTGGTTT GATGGTATGC TCGAGGCCGA CGAGGCTTAC TTCAAGGAGC ACGGAGAGCC CCTTTTCTCC TCCCACATGC TTGACTTGTC TGAGGAGTCC AAGGAGGACA ACATCAAGGA CTGTGTCTTC TACTTCAAGC GAATGGCCAA GGTTAACCTC TGGCTTGAAA TGGAAATCGG TATCACCGGT GGTGAGGAGG ACGGTGTTGA CAACACCTCT GTCGACAACA ACTCTCTCTA CACCCAGCCC GAGGACATCT GGGATGTCTA CTCTGCCCTT AACGCCATCT CTCCCAACTT CTCTATCGCT GCCGGTTTTG GTAACGTCCA CGGTGTTTAC AAGCCCGGAA ACGTCAAGCT CAGGCCTGAG CTCCTCGGCA AGCACCAGAA GTACACCCAC GAGCAGATCG GCGGTGACGA GGAGAAGCCC TTGTACCTTG TCTTCCACGG TGGTTCCGGT TCTACCAAGG ATGAGATCCG AGAGGCTGTT GTCAACGGCG TTGTTAAGAT GAACGTTGAC ACTGACACCC AATGGGCTTA CCTCTGCGGT GTCCGTGACT TCATCCTCAA GAAGAAGGAC TACCTCATGA CTCAGGTTGG TAACCCCGAT GGTGCCGACA AGCCCAACAA GAAGCAGTAC GACCCCCGAG TCTGGGTTAG GGAGGGTGAG AAGACTCTCG TCGAGCGAGT CAAGGAAGCC TGTGTCGACT TGGGCAACAA AAACAGGAAC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT