SSGCID: Target Page

CrneC.01391.b

NEK protein kinase

CENTER ID:	CrneC.01391.b
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.01391.b.B2.GE41333	Truncation 1		1	320

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_04148
UniProt:	J9VRL4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAVPRRSAGV ASSSGYADVA ELEKYKLVSN IGKGSFGVIS KVQRVSDGKE FALKQLDYSK MTEKDRKQIL AEVAILDSLK HRNIVQLIQK IKDPKNERIY IVMEYCTSGD LGTLIRRAQR NNSFLPEDKI WNIFLQIVLA LHHCHWPAER LANTGGRQSV VAPSTDGGVP RYQVLHRDLK PENVFLSDEF VKLGDFGLSK DMGTASFTST YVGTPLYMPP EILAENRYDT KSDIWSLGCL VYEMCALHSP FSAAQTQPEL ITMVKSGKIP PLPARYSSAL RSVIKAMLTL NPTKRPSTKD LLEMPEMKLH RKLFTVQNQT SLLFAKRDEL KQFEDQIRAR AVALDEREKE LAEREASLQA REELCESREE EAKETQRKLN QAAESLRGQW ERFRDEKEQW EKYREQEEKA KETAFGVTDV PDEKCQPHTG VVGIARPPLE ERITAPASPS VSRFTRCFDT PSKIPLATAA VSPAPLAPLE ARFGTSHFQP RPATPLRRAA TKSMGNLAGV TRAQAAQEWA DATQSTPAKQ FSFPMRQQRT SIGSPSELHN VYCEDVSMIS AIPSPWAPRP RKSSVAPSIL VAQQECSASG ELLSTANNRL ASVAPTQIPA PTFTYREAAT PAKWSLDDPD LPSPFIRRPN SMPTQPQAAS FPPSSNLAEM RQPLGSINPQ PTITASFASL GGPMAKTISN RGGNLHQHVL KVNAARVSGE GVAPSVASIG VVRGRAGNRI G

NT Sequence

atggcggttc ctcgacgatc tgctggtgtc gcttccagtt ctggatatgc cgacgtagca gagctggaga agtataagct agtgtcaaat attggcaaag gaagttttgg agtcatcagc aaagttcaga gagtgtcgga tggcaaggaa ttcgctctca aacaactgga ctattcgaag atgacagaga aagaccgtaa acaaatcctt gccgaggtag ccatcctcga ctcactcaag catcgaaata tcgtacagct cattcagaag attaaggacc ctaagaacga gcggatatat atagtcatgg agtattgcac ctcaggtgat cttggtaccc tgatcagaag agcccaacgc aataattctt tcctcccaga agacaagata tggaatatct ttctgcaaat tgtacttgct cttcatcact gtcattggcc agcagaacgc ctcgccaata ctggaggccg gcaaagtgtt gttgctccgt cgacggatgg cggagtaccc agatatcaag tgttacatcg tgacctcaaa ccggaaaatg tatttctttc cgacgaattt gtcaaattgg gagatttcgg gttgagtaaa gacatgggaa ctgcatcatt caccagcacc tacgtcggca ctcctctata tatgcctcca gaaattcttg cagagaaccg atatgataca aagtcagata tatggagctt gggatgtttg gtctacgaga tgtgtgctct tcactcccca ttctctgccg ctcaaacaca gcctgaactc ataaccatgg taaaatctgg caagataccc cctcttccgg ctcgttactc ctcagcttta cgtagtgtca ttaaagctat gcttactttg aaccctacaa aacgaccctc taccaaggat ctcctagaga tgcctgagat gaagttacat cggaaactct tcacagttca gaaccagact tccctactct ttgccaaacg tgacgagctc aaacaattcg aagatcagat cagagcacgg gctgtagcct tggacgaaag agagaaggag ctggccgaga gagaagcaag tttgcaggct agggaagaac tttgtgagag tcgagaagag gaagcaaagg aaactcagag aaagttgaat caagccgcgg agagtctgag aggccagtgg gagaggttcc gggacgaaaa ggaacagtgg gaaaaataca gagagcagga ggagaaggcc aaggaaacag ctttcggagt gacagatgtg cctgatgaga aatgtcaacc ccatactggt gtagtaggta tcgctcggcc tcccttagaa gaacgcatca ctgcaccagc atcaccttcc gtgtctcgat tcactcgctg ttttgatacc ccttcgaaaa tccctctagc cactgctgcc gtctctcctg cccctcttgc tcctcttgaa gcacgttttg gtacttctca ctttcaacct cgacctgcca ctcctctccg tcgagctgcc accaaatcaa tgggtaacct tgccggtgtt actcgtgctc aagcggccca agaatgggcg gatgctaccc aatcaacccc tgccaagcag ttcagcttcc ccatgcggca gcagcgcaca tctatcggta gccctagcga gctgcacaat gtgtattgtg aagatgtttc catgattagc gccattccct ctccttgggc accccgacct cgaaaaagct ctgtcgctcc ctctattctc gtcgctcagc aagagtgttc tgcttctggc gaattacttt ctaccgccaa caaccgtcta gcaagtgtag cacccacgca gatcccggct ccgacattta cctatcgtga agctgccact cccgccaaat ggtcattgga tgaccctgac cttcctagcc cattcattcg tcgtcccaat tcaatgccga cacaacccca agcggcatct ttccctccct cgtcaaatct ggccgaaatg agacagcctt taggctcaat caaccctcaa cctaccatca ccgcctcttt tgcttcttta ggaggtccta tggcaaagac catatcgaac aggggtggta atttacacca gcatgtcttg aaggtcaacg cggcgagagt gagtggggaa ggggtcgcgc cctctgttgc ctctatcggt gtggtgagag gaagggctgg gaatcgtatt gggtga

Details for CrneC.01391.b.B2.GE41333

HARVESTED ON:	data unavailable
SEQUENCED ON:	4/10/2017
EXPECTED MW:	37kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	92
PERCENT COVERAGE:	80

Validated AA Sequence

MAHHHHHHMA VPRRSAGVAS SSGYADVAEL EKYKLVSNIG KGSFGVISKV QRVSDGKEFA LKQLDYSKMT EKDRKQILAE VAILDSLKHR NIVQLIQKIK DPKNERIYIV MEYCTSGDLG TLIRRAQRNN SFLPEDKIWN IFLQIVLALH HCHWPAERLA NTGGRQSVVA PSTDGGVPRY QVLHRDLKPE NVFLSDEFVK LGDFGLSKDM GTASFTSTYV GTPLYMPPEI LAENRYDKVR YMELGMFGLR DVCSSLPILC RSNTA

Validated NT Sequence

ttactcactt aagtctggtt ctgaactgnn nnnnntttcc gatgtaactt catctcaggc atctctagga gatccttggt agagggtcgt tttgtagggt tcaaagtaag catagcttta atgacactac gtaaagctga ggagtaacga gccggaagag ggggtatctt gccagatttt accatggtta tgagttcagg ctgtgtttga gcggcagaga atggggagtg aagagcacac atctcgtaga ccaaacatcc caagctccat atatctgact ttgtcatatc ggttctctgc aagaatttct ggaggcatat atagaggagt gccgacgtag gtgctggtga atgatgcagt tcccatgtct ttactcaacc cgaaatctcc caatttgaca aattcgtcgg aaagaaatac attttccggt ttgaggtcac gatgtaacac ttgatatctg ggtactccgc catccgtcga cggagcaaca acactttgcc ggcctccagt attggcgagg cgttctgctg gccaatgaca gtgatgaaga gcaagtacaa tttgcagaaa gatattccat atcttgtctt ctgggaggaa agaattattg cgttgggctc ttctgatcag ggtaccaaga tcacctgagg tgcaatactc catgactata tatatccgct cgttcttagg gtccttaatc ttctgaatga gctgtacgat atttcgatgc ttgagtgagt cgaggatggc tacctcggca aggatttgtt tacggtcttt ctctgtcatc ttcgaatagt ccagttgttt gagagcgaat tccttgccat ccgacactct ctgaactttg ctgatgactc caaaacttcc tttgccaata tttgacacta gcttatactt ctccagctct gctacgtcgg catatccaga actggaagcg acaccagcag atcgtcgagg aaccgccata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg ttcctcgacg atctgctggt gtcgcttcca gttctggata tgccgacgta gcagagctgg agaagtataa gctagtgtca aatattggca aaggaagttt tggagtcatc agcaaagttc agagagtgtc ggatggcaag gaattcgctc tcaaacaact ggactattcg aagatgacag agaaagaccg taaacaaatc cttgccgagg tagccatcct cgactcactc aagcatcgaa atatcgtaca gctcattcag aagattaagg accctaagaa cgagcggata tatatagtca tggagtattg cacctcaggt gatcttggta ccctgatcag aagagcccaa cgcaataatt ctttcctccc agaagacaag atatggaata tctttctgca aattgtactt gctcttcatc actgtcattg gccagcagaa cgcctcgcca atactggagg ccggcaaagt gttgttgctc cgtcgacgga tggcggagta cccagatatc aagtgttaca tcgtgacctc aaaccggaaa atgtatttct ttccgacgaa tttgtcaaat tgggagattt cgggttgagt aaagacatgg gaactgcatc attcaccagc acctacgtcg gcactcctct atatatgcct ccagaaattc ttgcagagaa ccgatatgat acaaagtcag atatatggag cttgggatgt ttggtctacg agatgtgtgc tcttcactcc ccattctctg ccgctcaaac acagcctgaa ctcataacca tggtaaaatc tggcaagata ccccctcttc cggctcgtta ctcctcagct ttacgtagtg tcattaaagc tatgcttact ttgaacccta caaaacgacc ctctaccaag gatctcctag agatgcctga gatgaagtta catcggaaac tcttcacagt tcagaaccag acttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat