CrneC.01291.b

Adenylate kinase (EC 2.7.4.3) (ATP-AMP transphosphorylase) (ATP:AMP phosphotransferase) (Adenylate kinase cytosolic and mitochondrial) (Adenylate monophosphate kinase)

CENTER ID: CrneC.01291.b
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.01291.b.B1.GE41328 full length 1 269
CrneC.01291.b.B2.GE41329 Truncation 1 54 269
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.01291.b.B2.PW38303 Truncation 1 54 269
CrneC.01291.b.B2.PW38304 Truncation 1 54 269
External Resources
RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_02007
UniProt: J9VVB2
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGNPEVEYL KSLVSQLQDK IHQLEKSTST SVSNTISSVT SALSPSSSSK PPRMVLIGPP GAGKGTQAPN ISSKYCICHL ATGDMLREQV SKQTELGKAA KQIMDQGGLV SDEIMVGMIK QELEKNAECK HGFILDGFPR TVPQASKLDA MLAERKQAID HAIELKIPDA LLISRITGRL VHPASGRSYH REFNPPKKPM TDDITGEPLI QRSDDNVGTL RKRLDTYHAQ TGPVVDYYKG TGVWTPVDAA QSPKLVWASI SSILESKKN
NT Sequence
atgtctggca accctgaagt tgaatatttg aagtctcttg tctctcagct ccaagacaag atccatcaac tcgagaagtc tacctccacc tccgtctcca acaccatctc ctccgtcacc tccgccctct ctccctcttc ctccagcaag cctccccgaa tggtcctcat cggccctccc ggtgctggta agggtactca agcgcccaac atctctagca aatactgcat ctgtcacctc gctactggtg acatgttgag ggagcaagtt tccaagcaga ccgaattggg taaggctgct aaacagatta tggaccaggg cggtttggtt tccgatgaga ttatggttgg catgatcaag caggagttgg agaagaacgc cgagtgcaag catggcttca tcctcgacgg cttcccccgt accgtccccc aggcttccaa gctcgatgcc atgcttgccg agcgcaaaca agctatcgac cacgctatcg agctcaagat ccccgacgcc ttgttgatct cccgaattac tggtcgattg gtccaccccg cgagcggtag gagttaccac agggaattca acccccctaa gaagcccatg actgatgaca tcaccggcga gcccctcata caacgttccg acgacaacgt cggtaccctc aggaagcgat tggacactta ccacgcccag actggccccg ttgttgacta ttacaagggt actggtgttt ggactcctgt cgatgccgct cagagcccca agttggtctg ggctagtatc agcagtatct tggaaagcaa gaagaactaa
Details for CrneC.01291.b.B1.GE41328
HARVESTED ON: 4/3/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS GNPEVEYLKS LVSQLQDKIH QLEKSTSTSV SNTISSVTSA LSPSSSSKPP RMVLIGPPGA GKGTQAPNIS SKYCICHLAT GDMLREQVSK QTELGKAAKQ IMDQGGLVSD EIMVGMIKQE LEKNAECKHG FILDGFPRTV PQASKLDAML AERKQAIDHA IELKIPDALL ISRITGRLVH PASGRSYHRE FNPPKKPMTD DITGEPLIQR SDDNVGTLRK RLDTYHAQTG PVVDYYKGTG VWTPVDAAQS PKLVWASISS IXESKKN
Validated NT Sequence
atcttactca cttagttctt cttgctttcn nagatactgc tgatactagc ccagaccaac ttggggctct gagcggcatc gacaggagtc caaacaccag tacccttgta atagtcaaca acggggccag tctgggcgtg gtaagtgtcc aatcgcttcc tgagggtacc gacgttgtcg tcggaacgtt gtatgagggg ctcgccggtg atgtcatcag tcatgggctt cttagggggg ttgaattccc tgtggtaact cctaccgctc gcggggtgga ccaatcgacc agtaattcgg gagatcaaca aggcgtcggg gatcttgagc tcgatagcgt ggtcgatagc ttgtttgcgc tcggcaagca tggcatcgag cttggaagcc tgggggacgg tacgggggaa gccgtcgagg atgaagccat gcttgcactc ggcgttcttc tccaactcct gcttgatcat gccaaccata atctcatcgg aaaccaaacc gccctggtcc ataatctgtt tagcagcctt acccaattcg gtctgcttgg aaacttgctc cctcaacatg tcaccagtag cgaggtgaca gatgcagtat ttgctagaga tgttgggcgc ttgagtaccc ttaccagcac cgggagggcc gatgaggacc attcggggag gcttgctgga ggaagaggga gagagggcgg aggtgacgga ggagatggtg ttggagacgg aggtggaggt agacttctcg agttgatgga tcttgtcttg gagctgagag acaagagact tcaaatattc aacttcaggg ttgccagaca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMS GNPEVEYLKS LVSQLQDKIH QLEKSTSTSV SNTISSVTSA LSPSSSSKPP RMVLIGPPGA GKGTQAPNIS SKYCICHLAT GDMLREQVSK QTELGKAAKQ IMDQGGLVSD EIMVGMIKQE LEKNAECKHG FILDGFPRTV PQASKLDAML AERKQAIDHA IELKIPDALL ISRITGRLVH PASGRSYHRE FNPPKKPMTD DITGEPLIQR SDDNVGTLRK RLDTYHAQTG PVVDYYKGTG VWTPVDAAQS PKLVWASISS ILESKKN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg gcaaccctga agttgaatat ttgaagtctc ttgtctctca gctccaagac aagatccatc aactcgagaa gtctacctcc acctccgtct ccaacaccat ctcctccgtc acctccgccc tctctccctc ttcctccagc aagcctcccc gaatggtcct catcggccct cccggtgctg gtaagggtac tcaagcgccc aacatctcta gcaaatactg catctgtcac ctcgctactg gtgacatgtt gagggagcaa gtttccaagc agaccgaatt gggtaaggct gctaaacaga ttatggacca gggcggtttg gtttccgatg agattatggt tggcatgatc aagcaggagt tggagaagaa cgccgagtgc aagcatggct tcatcctcga cggcttcccc cgtaccgtcc cccaggcttc caagctcgat gccatgcttg ccgagcgcaa acaagctatc gaccacgcta tcgagctcaa gatccccgac gccttgttga tctcccgaat tactggtcga ttggtccacc ccgcgagcgg taggagttac cacagggaat tcaacccccc taagaagccc atgactgatg acatcaccgg cgagcccctc atacaacgtt ccgacgacaa cgtcggtacc ctcaggaagc gattggacac ttaccacgcc cagactggcc ccgttgttga ctattacaag ggtactggtg tttggactcc tgtcgatgcc gctcagagcc ccaagttggt ctgggctagt atcagcagta tcttggaaag caagaagaac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for CrneC.01291.b.B2.GE41329
HARVESTED ON: 4/3/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV LIGPPGAGKG TQAPNISSKY CICHLATGDM LREQVSKQTE LGKAAKQIMD QGGLVSDEIM VGMIKQELEK NAECKHGFIL DGFPRTVPQA SKLDAMLAER KQAIDHAIEL KIPDALLISR ITGRLVHPAS GRSYHREFNP PKKPMTDDIT GEPLIQRSDD NVGTLRKRLD TYHAQTGPVV DYYKGTGVWT PVDAAQSPKL VWASISSILE SKKN
Validated NT Sequence
atcttactca cttagttctt cttgctttcc aagatactgc tgatactagc ccagaccaac ttggggctct gagcggcatc gacaggagtc caaacaccag tacccttgta atagtcaaca acggggccag tctgggcgtg gtaagtgtcc aatcgcttcc tgagggtacc gacgttgtcg tcggaacgtt gtatgagggg ctcgccggtg atgtcatcag tcatgggctt cttagggggg ttgaattccc tgtggtaact cctaccgctc gcggggtgga ccaatcgacc agtaattcgg gagatcaaca aggcgtcggg gatcttgagc tcgatagcgt ggtcgatagc ttgtttgcgc tcggcaagca tggcatcgag cttggaagcc tgggggacgg tacgggggaa gccgtcgagg atgaagccat gcttgcactc ggcgttcttc tccaactcct gcttgatcat gccaaccata atctcatcgg aaaccaaacc gccctggtcc ataatctgtt tagcagcctt acccaattcg gtctgcttgg aaacttgctc cctcaacatg tcaccagtag cgaggtgaca gatgcagtat ttgctagaga tgttgggcgc ttgagtaccc ttaccagcac cgggagggcc gatgaggacc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMV LIGPPGAGKG TQAPNISSKY CICHLATGDM LREQVSKQTE LGKAAKQIMD QGGLVSDEIM VGMIKQELEK NAECKHGFIL DGFPRTVPQA SKLDAMLAER KQAIDHAIEL KIPDALLISR ITGRLVHPAS GRSYHREFNP PKKPMTDDIT GEPLIQRSDD NVGTLRKRLD TYHAQTGPVV DYYKGTGVWT PVDAAQSPKL VWASISSILE SKKN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtcc tcatcggccc tcccggtgct ggtaagggta ctcaagcgcc caacatctct agcaaatact gcatctgtca cctcgctact ggtgacatgt tgagggagca agtttccaag cagaccgaat tgggtaaggc tgctaaacag attatggacc agggcggttt ggtttccgat gagattatgg ttggcatgat caagcaggag ttggagaaga acgccgagtg caagcatggc ttcatcctcg acggcttccc ccgtaccgtc ccccaggctt ccaagctcga tgccatgctt gccgagcgca aacaagctat cgaccacgct atcgagctca agatccccga cgccttgttg atctcccgaa ttactggtcg attggtccac cccgcgagcg gtaggagtta ccacagggaa ttcaaccccc ctaagaagcc catgactgat gacatcaccg gcgagcccct catacaacgt tccgacgaca acgtcggtac cctcaggaag cgattggaca cttaccacgc ccagactggc cccgttgttg actattacaa gggtactggt gtttggactc ctgtcgatgc cgctcagagc cccaagttgg tctgggctag tatcagcagt atcttggaaa gcaagaagaa ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for CrneC.01291.b.B2.PW38303
PURIFICATION DATe: 6/13/2017
CONCENTRATION: 6.2mg/ml
OBSERVED MW: 26kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV LIGPPGAGKG TQAPNISSKY CICHLATGDM LREQVSKQTE LGKAAKQIMD QGGLVSDEIM VGMIKQELEK NAECKHGFIL DGFPRTVPQA SKLDAMLAER KQAIDHAIEL KIPDALLISR ITGRLVHPAS GRSYHREFNP PKKPMTDDIT GEPLIQRSDD NVGTLRKRLD TYHAQTGPVV DYYKGTGVWT PVDAAQSPKL VWASISSILE SKKN
Validated NT Sequence
atcttactca cttagttctt cttgctttcc aagatactgc tgatactagc ccagaccaac ttggggctct gagcggcatc gacaggagtc caaacaccag tacccttgta atagtcaaca acggggccag tctgggcgtg gtaagtgtcc aatcgcttcc tgagggtacc gacgttgtcg tcggaacgtt gtatgagggg ctcgccggtg atgtcatcag tcatgggctt cttagggggg ttgaattccc tgtggtaact cctaccgctc gcggggtgga ccaatcgacc agtaattcgg gagatcaaca aggcgtcggg gatcttgagc tcgatagcgt ggtcgatagc ttgtttgcgc tcggcaagca tggcatcgag cttggaagcc tgggggacgg tacgggggaa gccgtcgagg atgaagccat gcttgcactc ggcgttcttc tccaactcct gcttgatcat gccaaccata atctcatcgg aaaccaaacc gccctggtcc ataatctgtt tagcagcctt acccaattcg gtctgcttgg aaacttgctc cctcaacatg tcaccagtag cgaggtgaca gatgcagtat ttgctagaga tgttgggcgc ttgagtaccc ttaccagcac cgggagggcc gatgaggacc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMV LIGPPGAGKG TQAPNISSKY CICHLATGDM LREQVSKQTE LGKAAKQIMD QGGLVSDEIM VGMIKQELEK NAECKHGFIL DGFPRTVPQA SKLDAMLAER KQAIDHAIEL KIPDALLISR ITGRLVHPAS GRSYHREFNP PKKPMTDDIT GEPLIQRSDD NVGTLRKRLD TYHAQTGPVV DYYKGTGVWT PVDAAQSPKL VWASISSILE SKKN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTCC TCATCGGCCC TCCCGGTGCT GGTAAGGGTA CTCAAGCGCC CAACATCTCT AGCAAATACT GCATCTGTCA CCTCGCTACT GGTGACATGT TGAGGGAGCA AGTTTCCAAG CAGACCGAAT TGGGTAAGGC TGCTAAACAG ATTATGGACC AGGGCGGTTT GGTTTCCGAT GAGATTATGG TTGGCATGAT CAAGCAGGAG TTGGAGAAGA ACGCCGAGTG CAAGCATGGC TTCATCCTCG ACGGCTTCCC CCGTACCGTC CCCCAGGCTT CCAAGCTCGA TGCCATGCTT GCCGAGCGCA AACAAGCTAT CGACCACGCT ATCGAGCTCA AGATCCCCGA CGCCTTGTTG ATCTCCCGAA TTACTGGTCG ATTGGTCCAC CCCGCGAGCG GTAGGAGTTA CCACAGGGAA TTCAACCCCC CTAAGAAGCC CATGACTGAT GACATCACCG GCGAGCCCCT CATACAACGT TCCGACGACA ACGTCGGTAC CCTCAGGAAG CGATTGGACA CTTACCACGC CCAGACTGGC CCCGTTGTTG ACTATTACAA GGGTACTGGT GTTTGGACTC CTGTCGATGC CGCTCAGAGC CCCAAGTTGG TCTGGGCTAG TATCAGCAGT ATCTTGGAAA GCAAGAAGAA CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT
Details for CrneC.01291.b.B2.PW38304
PURIFICATION DATe: 6/13/2017
CONCENTRATION: 12.8mg/ml
OBSERVED MW: 26kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV LIGPPGAGKG TQAPNISSKY CICHLATGDM LREQVSKQTE LGKAAKQIMD QGGLVSDEIM VGMIKQELEK NAECKHGFIL DGFPRTVPQA SKLDAMLAER KQAIDHAIEL KIPDALLISR ITGRLVHPAS GRSYHREFNP PKKPMTDDIT GEPLIQRSDD NVGTLRKRLD TYHAQTGPVV DYYKGTGVWT PVDAAQSPKL VWASISSILE SKKN
Validated NT Sequence
atcttactca cttagttctt cttgctttcc aagatactgc tgatactagc ccagaccaac ttggggctct gagcggcatc gacaggagtc caaacaccag tacccttgta atagtcaaca acggggccag tctgggcgtg gtaagtgtcc aatcgcttcc tgagggtacc gacgttgtcg tcggaacgtt gtatgagggg ctcgccggtg atgtcatcag tcatgggctt cttagggggg ttgaattccc tgtggtaact cctaccgctc gcggggtgga ccaatcgacc agtaattcgg gagatcaaca aggcgtcggg gatcttgagc tcgatagcgt ggtcgatagc ttgtttgcgc tcggcaagca tggcatcgag cttggaagcc tgggggacgg tacgggggaa gccgtcgagg atgaagccat gcttgcactc ggcgttcttc tccaactcct gcttgatcat gccaaccata atctcatcgg aaaccaaacc gccctggtcc ataatctgtt tagcagcctt acccaattcg gtctgcttgg aaacttgctc cctcaacatg tcaccagtag cgaggtgaca gatgcagtat ttgctagaga tgttgggcgc ttgagtaccc ttaccagcac cgggagggcc gatgaggacc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMV LIGPPGAGKG TQAPNISSKY CICHLATGDM LREQVSKQTE LGKAAKQIMD QGGLVSDEIM VGMIKQELEK NAECKHGFIL DGFPRTVPQA SKLDAMLAER KQAIDHAIEL KIPDALLISR ITGRLVHPAS GRSYHREFNP PKKPMTDDIT GEPLIQRSDD NVGTLRKRLD TYHAQTGPVV DYYKGTGVWT PVDAAQSPKL VWASISSILE SKKN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTCC TCATCGGCCC TCCCGGTGCT GGTAAGGGTA CTCAAGCGCC CAACATCTCT AGCAAATACT GCATCTGTCA CCTCGCTACT GGTGACATGT TGAGGGAGCA AGTTTCCAAG CAGACCGAAT TGGGTAAGGC TGCTAAACAG ATTATGGACC AGGGCGGTTT GGTTTCCGAT GAGATTATGG TTGGCATGAT CAAGCAGGAG TTGGAGAAGA ACGCCGAGTG CAAGCATGGC TTCATCCTCG ACGGCTTCCC CCGTACCGTC CCCCAGGCTT CCAAGCTCGA TGCCATGCTT GCCGAGCGCA AACAAGCTAT CGACCACGCT ATCGAGCTCA AGATCCCCGA CGCCTTGTTG ATCTCCCGAA TTACTGGTCG ATTGGTCCAC CCCGCGAGCG GTAGGAGTTA CCACAGGGAA TTCAACCCCC CTAAGAAGCC CATGACTGAT GACATCACCG GCGAGCCCCT CATACAACGT TCCGACGACA ACGTCGGTAC CCTCAGGAAG CGATTGGACA CTTACCACGC CCAGACTGGC CCCGTTGTTG ACTATTACAA GGGTACTGGT GTTTGGACTC CTGTCGATGC CGCTCAGAGC CCCAAGTTGG TCTGGGCTAG TATCAGCAGT ATCTTGGAAA GCAAGAAGAA CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT