CrneC.01252.b

AGC/PKA protein kinase

CENTER ID: CrneC.01252.b
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.01252.b.B2.GE41325 Truncation 1 195 515
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_00396
UniProt: J9VIW8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFQKVSDKFH RKQQSSTSPG KTQQVPNSPS SVLAKANSQA QQAYSSQDHS PMEGIQSDST HIQQPMATQK APIVGPSTST SLSTVPVQDG TLPLTPGAQG MLAGTTDGHR QVQSPVSRSS SAGEDKMREK ARDAQEQAAQ AQANLHRVTQ QARVAAINAA ATQAALETAT QLPATARVPT SGTGAEPGQA RRKTAGRYAL SDFLIERTLG TGSFGRVHLV RSRHNGRFYA VKVLNKEKVI KMKQVEHTNS EREMLVRVRH PFLVNLWGTF QDVNNLYMVM DFVAGGELFS LLRKSQRFPN SVAKFYAAEV ALALDYLHSL DIIYRDLKPE NLLLGADGHV KVTDFGFAKY VPDITWTLCG TPDYLAPEVV QSKGYNKSVD WYALGVLIFE MLAGYPPFFT EDGNPMKLYE KIIAGKVRYP TYFDVLAKEL LKNLLIGDLT KRYGNLRAGS SDIFAHGWFA EVDWDKLYRR EIPAPYVPKI DGEGDASQFD RYQEADVSAY GKVGNGPYDH FFVEF
NT Sequence
atgttccaaa aggtgtccga taagttccat cgcaagcagc agtcgtcgac atcacccggt aaaactcaac aggttcctaa ttctcctagc tcagtcctgg cgaaagccaa ttcccaagct caacaagcat attcttccca agatcattcc ccaatggaag gtatacaaag tgattccacg catatccaac agcccatggc tactcaaaaa gcacccatag tcggaccttc tacatcgaca tctttatcca ccgtacccgt gcaagatggc acccttccat taactccagg tgcacaaggg atgttggcag gcacaaccga cgggcatcgg caagttcaga gtcctgtatc tcgtagtagt agtgcaggtg aggataagat gagggaaaag gcgagggatg cccaggagca ggcagcgcaa gctcaagcca acctccatcg cgtaacgcaa caagctcgcg ttgccgccat caatgctgcg gctactcaag ctgcccttga aacagccacc cagctgcctg caactgctag ggtcccaact tccggtacag gagctgagcc gggtcaagca aggcgcaaaa cagctggccg atatgctcta tctgattttc ttattgaaag aactttgggc acggggtcct tcggtcgtgt gcatttagtg cggtcgcgcc acaacggccg tttctatgcc gttaaggttc tgaacaagga gaaggttatc aagatgaagc aagttgaaca caccaattct gaacgagaga tgttggtacg ggttcgacac ccatttctcg tcaatctctg gggaaccttc caggatgtaa ataacctgta tatggttatg gactttgtgg caggaggtga actttttagc cttcttcgta aaagtcagcg attcccgaat tctgtcgcga aattctacgc agccgaagtt gctttagcgc tcgattatct tcattcactc gatattatct accgagatct taagccggaa aatctacttt tgggtgcaga tggccatgtc aaggttaccg actttggttt cgccaagtat gtacctgata tcacctggac cctctgcggg acaccggatt accttgctcc ggaggttgtt cagtccaagg gctacaacaa aagcgtcgac tggtacgctt taggggtatt gatatttgaa atgctggcgg gttatcctcc cttcttcact gaagatggca atccaatgaa attgtatgag aagatcattg ctggcaaagt ccgctatccg acatactttg acgtacttgc gaaagaactc ttaaaaaatc tattgatcgg ggatctcact aagcgatacg gtaatttgag agcaggctct tctgatatat ttgcccatgg atggttcgcc gaagtcgatt gggacaagtt gtacaggcgc gaaattccgg ctccatatgt gcccaagatc gacggcgagg gagacgctag ccaattcgac cgatatcaag aggcggatgt tagtgcctac gggaaggtcg gcaatggtcc atatgatcat ttcttcgtgg agttttag
Details for CrneC.01252.b.B2.GE41325
HARVESTED ON: 4/3/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA GRYALSDFLI ERTLGTGSFG RVHLVRSRHN GRFYAVKVLN KEKVIKMKQV EHTNSEREML VRVRHPFLVN LWGTFQDVNN LYMVMDFVAG GELFSLLRKS QRFPNSVAKF YAAEVALALD YLHSLDIIYR DLKPENLLLG ADGHVKVTDF GFAKYVPDIT WTLCGTPDYL APEVVQSKGY NKSVDWYALG VLIFEMLAGY PPFFTEDGNP MKLYEKIIAG KVRYPTYFDV LAKELLKNLL IGDLTKRYGN LRAGSSDIFA HGWFAEVDWD KLYRREIPAP YVPKIDGEGD ASQFDRYQEA DVSAYGKVGN GPYDHFFVEF
Validated NT Sequence
ttaaaactcc acgaagaaat gatcatatgg accattgccg accttcccgt aggcactaac atccgcctct tgatatcggt cgaattggct agcgtctccc tcgccgtcga tcttgggcac atatggagcc ggaatttcgc gcctgtacaa cttgtcccaa tcgacttcgg cgaaccatcc atgggcaaat atatcagaag agcctgctct caaattaccg tatcgcttag tgagatcccc gatcaataga ttttttaaga gttctttcgc aagtacgtca aagtatgtcg gatagcggac tttgccagca atgatcttct catacaattt cattggattg ccatcttcag tgaagaaggg aggataaccc gccagcattt caaatatcaa tacccctaaa gcgtaccagt cgacgctttt gttgtagccc ttggactgaa caacctccgg agcaaggtaa tccggtgtcc cgcagagggt ccaggtgata tcaggtacat acttggcgaa accaaagtcg gtaaccttga catggccatc tgcacccaaa agtagatttt ccggcttaag atctcggtag ataatatcga gtgaatgaag ataatcgagc gctaaagcaa cttcggctgc gtagaatttc gcgacagaat tcgggaatcg ctgactttta cgaagaaggc taaaaagttc acctcctgcc acaaagtcca taaccatata caggttattt acatcctgga aggttcccca gagattgacg agaaatgggt gtcgaacccg taccaacatc tctcgttcag aattggtgtg ttcaacttgc ttcatcttga taaccttctc cttgttcaga accttaacgg catagaaacg gccgttgtgg cgcgaccgca ctaaatgcac acgaccgaag gaccccgtgc ccaaagttct ttcaataaga aaatcagata gagcatatcg gccagccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHAG RYALSDFLIE RTLGTGSFGR VHLVRSRHNG RFYAVKVLNK EKVIKMKQVE HTNSEREMLV RVRHPFLVNL WGTFQDVNNL YMVMDFVAGG ELFSLLRKSQ RFPNSVAKFY AAEVALALDY LHSLDIIYRD LKPENLLLGA DGHVKVTDFG FAKYVPDITW TLCGTPDYLA PEVVQSKGYN KSVDWYALGV LIFEMLAGYP PFFTEDGNPM KLYEKIIAGK VRYPTYFDVL AKELLKNLLI GDLTKRYGNL RAGSSDIFAH GWFAEVDWDK LYRREIPAPY VPKIDGEGDA SQFDRYQEAD VSAYGKVGNG PYDHFFVEF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctggcc gatatgctct atctgatttt cttattgaaa gaactttggg cacggggtcc ttcggtcgtg tgcatttagt gcggtcgcgc cacaacggcc gtttctatgc cgttaaggtt ctgaacaagg agaaggttat caagatgaag caagttgaac acaccaattc tgaacgagag atgttggtac gggttcgaca cccatttctc gtcaatctct ggggaacctt ccaggatgta aataacctgt atatggttat ggactttgtg gcaggaggtg aactttttag ccttcttcgt aaaagtcagc gattcccgaa ttctgtcgcg aaattctacg cagccgaagt tgctttagcg ctcgattatc ttcattcact cgatattatc taccgagatc ttaagccgga aaatctactt ttgggtgcag atggccatgt caaggttacc gactttggtt tcgccaagta tgtacctgat atcacctgga ccctctgcgg gacaccggat taccttgctc cggaggttgt tcagtccaag ggctacaaca aaagcgtcga ctggtacgct ttaggggtat tgatatttga aatgctggcg ggttatcctc ccttcttcac tgaagatggc aatccaatga aattgtatga gaagatcatt gctggcaaag tccgctatcc gacatacttt gacgtacttg cgaaagaact cttaaaaaat ctattgatcg gggatctcac taagcgatac ggtaatttga gagcaggctc ttctgatata tttgcccatg gatggttcgc cgaagtcgat tgggacaagt tgtacaggcg cgaaattccg gctccatatg tgcccaagat cgacggcgag ggagacgcta gccaattcga ccgatatcaa gaggcggatg ttagtgccta cgggaaggtc ggcaatggtc catatgatca tttcttcgtg gagttttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t