CrneC.01252.a

AGC/PKA protein kinase

CENTER ID:	CrneC.01252.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.01252.a.B3.GE41324	Truncation 2		185	514

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_04162
UniProt:	J9VR38

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDPPHASSIP SSSSSMPTPG SASTSANKPK KFKLDPMSML LPHERPGYRG SPAACRSMPP IHNQFQLQPQ PGSPAYFAML QSQQVQQDSA TQRIGIPTRT AASMGEQGGK RGESSGSQIP TQAPVGSQHP AQGVPLSPPA SMSSTPQSGS WEDSASQWSS MEGVVDGKPA SGQRSQPSRH YAPRMTQGNM RLEDWEIVET LGTGTFGRVL LVRQRPSYRP TPYHPIFPHL YRSRHPLSPS PSTTQKSDDH LPHFAMKVLR KSEIVRLKQV EHINSERIIL ERVRHPFIVE LHATYQDQIN VYMLLFYIPG GELFSHLRRA GRFSADVTRF YLASIVLAIE YLHSRNIIYR DLKPENLLLD RHGYLRIADF GFAKVVQDRT FTLCGTPEYL APEIVLSQGH GKAVDWWALG ILGFEMLAGY PPFFDDHPLG IYEKILRGDI AFPSHIDPFA KDLIRGLLTA DRSKRLGNLR GGAKDVMGHP WFAGVDWRSL ERKEIGAPIV PRVASMGDSQ NFQRYPPPRP QDLPGVFGQT YNTSSDPCGE LFTDFSFPRG ESERGSASGS AEVLGRQ

NT Sequence

atggatccac cccacgcatc ctccattccc tcctcatcct cctcaatgcc taccccaggc agcgcctcga cgtccgctaa taaacccaaa aagttcaagc ttgatccgat gtcaatgctt ttaccccatg aacgtccagg ctaccgtggt tctcctgccg cttgtcggag tatgcctccc atacataatc aattccaact acaaccacaa cccggttcac cggcgtattt tgcaatgttg cagagccagc aagtacagca agattctgcg acacaaagga tcgggatacc gacgcggaca gcggctagta tgggtgaaca gggtgggaag cgcggagaaa gcagtggcag tcagattcca acccaagctc cagttggttc acaacaccca gcacaaggtg taccattatc gcctccggcg tcaatgtcat caactccaca gtccgggtct tgggaggatt ccgcctctca gtggagctca atggagggtg ttgtcgacgg caagcctgca tccggacaac ggtcccagcc tagccggcat tacgccccgc ggatgactca gggcaacatg aggttggaag attgggaaat cgtcgagacg ctgggtactg ggacctttgg acgagtttta cttgttcgac aacgaccttc ttatcgaccg acgccatatc accccatttt cccccatctc taccgatctc gtcatcctct ctcgccatca ccttccacaa cgcaaaaatc cgatgatcat ctccctcatt tcgctatgaa agtccttcgc aagtcagaaa ttgtgaggtt gaagcaagtg gaacacatca actctgagcg gattattctt gaacgtgtac gacatccatt tatcgtcgaa ctgcatgcca cctaccagga ccagatcaat gtctatatgc tcttatttta cattccaggt ggcgaacttt tttcgcatct tcgacgggca ggacggtttt ccgccgatgt caccaggttc tatctcgcgt ctatcgtcct tgccattgaa tacctccatt cgcgaaacat tatctaccgt gatctcaaac ccgaaaacct ccttctcgat cgtcatggct accttcgtat cgcggatttt ggctttgcca aagtggtgca agaccgtaca ttcactttat gtggtacacc ggaatatctc gctccagaga tcgttctctc acaaggccac ggcaaagctg tcgactggtg ggcgcttggt atcctcggat tcgaaatgct tgctggctat cccccttttt ttgatgacca tcctctcggt atctacgaaa agattcttcg cggagatatc gcgttccctt cacatatcga tcctttcgcc aaagatctca ttagggggct tttgacagcg gatcgaagta agaggttggg gaacttgaga ggtggcgcga aggatgtaat gggacatccc tggtttgcgg gtgtagattg gagaagccta gagaggaagg aaattggagc tccgatcgtc cctcgggtgg ctagcatggg cgattcgcag aatttccagc ggtatccacc tccccgaccg caagaccttc cgggtgtatt cggccaaaca tacaacacat cgagcgatcc atgtggcgaa ctatttacag acttcagttt ccctagagga gagagcgaga ggggatctgc gagcgggtcc gctgaggttt tgggtcggca gtag

Details for CrneC.01252.a.B3.GE41324

HARVESTED ON:	4/3/2017
SEQUENCED ON:	4/10/2017
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMT QGNMRLEDWE IVETLGTGTF GRVLLVRQRP SYRPTPYHPI FPHLYRSRHP LSPSPSTTQK SDDHLPHFAM KVLRKSEIVR LKQVEHINSE RIILERVRHP FIVELHATYQ DQINVYMLLF YIPGGELFSH LRRAGRFSAD VTRFYLASIV LAIEYLHSRN IIYRDLKPEN LLLDRHGYLR IADFGFAKVV QDRTFTLCGT PEYLAPEIVL SQGHGKAVDW WALGILGFEM LAGYPPFFDD HPLGIYEKIL RGDIAFPSHI DPFAKDLIRG LLTADRSKRL GNLRGGAKDV MGHPWFAGVD WRSLERKEIG APIVPRVAS

Validated NT Sequence

tgctagccac ccgagggacg atcggagctc caatttcctt cctctctagg cttctccaat ctacacccgc aaaccaggga tgtcccatta catccttcgc gccacctctc aagttcccca acctcttact tcgatccgct gtcaaaagcc ccctaatgag atctttggcg aaaggatcga tatgtgaagg gaacgcgata tctccgcgaa gaatcttttc gtagataccg agaggatggt catcaaaaaa agggggatag ccagcaagca tttcgaatcc gaggatacca agcgcccacc agtcgacagc tttgccgtgg ccttgtgaga gaacgatctc tggagcgaga tattccggtg taccacataa agtgaatgta cggtcttgca ccactttggc aaagccaaaa tccgcgatac gaaggtagcc atgacgatcg agaaggaggt tttcgggttt gagatcacgg tagataatgt ttcgcgaatg gaggtattca atggcaagga cgatagacgc gagatagaac ctggtgacat cggcggaaaa ccgtcctgcc cgtcgaagat gcgaaaaaag ttcgccacct ggaatgtaaa ataagagcat atagacattg atctggtcct ggtaggtggc atgcagttcg acgataaatg gatgtcgtac acgttcaaga ataatccgct cagagttgat gtgttccact tgcttcaacc tcacaatttc tgacttgcga aggactttca tagcgaaatg agggagatga tcatcggatt tttgcgttgt ggaaggtgat ggcgagagag gatgacgaga tcggtagaga tgggggaaaa tggggtgata tggcgtcggt cgataagaag gtcgttgtcg aacaagtaaa actcgtccaa aggtcccagt acccagcgtc tcgacgattt cccaatcttc caacctcatg ttgccctgag tcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactc agggcaacat gaggttggaa gattgggaaa tcgtcgagac gctgggtact gggacctttg gacgagtttt acttgttcga caacgacctt cttatcgacc gacgccatat caccccattt tcccccatct ctaccgatct cgtcatcctc tctcgccatc accttccaca acgcaaaaat ccgatgatca tctccctcat ttcgctatga aagtccttcg caagtcagaa attgtgaggt tgaagcaagt ggaacacatc aactctgagc ggattattct tgaacgtgta cgacatccat ttatcgtcga actgcatgcc acctaccagg accagatcaa tgtctatatg ctcttatttt acattccagg tggcgaactt ttttcgcatc ttcgacgggc aggacggttt tccgccgatg tcaccaggtt ctatctcgcg tctatcgtcc ttgccattga atacctccat tcgcgaaaca ttatctaccg tgatctcaaa cccgaaaacc tccttctcga tcgtcatggc taccttcgta tcgcggattt tggctttgcc aaagtggtgc aagaccgtac attcacttta tgtggtacac cggaatatct cgctccagag atcgttctct cacaaggcca cggcaaagct gtcgactggt gggcgcttgg tatcctcgga ttcgaaatgc ttgctggcta tccccctttt tttgatgacc atcctctcgg tatctacgaa aagattcttc gcggagatat cgcgttccct tcacatatcg atcctttcgc caaagatctc attagggggc ttttgacagc ggatcgaagt aagaggttgg ggaacttgag aggtggcgcg aaggatgtaa tgggacatcc ctggtttgcg ggtgtagatt ggagaagcct agagaggaag gaaattggag ctccgatcgt ccctcgggtg gctagcatgg gcgattcgca gaatttccag cggtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat