CrneC.01218.a

Nucleoside-diphosphate kinase

CENTER ID: CrneC.01218.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.01218.a.B1.GE41321 full length 1 223
CrneC.01218.a.B2.GE41322 Truncation 1 69 223
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_04577
UniProt: J9W0Q9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFANSLRHGL RTASRSSARA FSTIPARAPR TNVVGALALG TAVTGYALYE STRSPVLLEG PRTIAGEKGT VTERSFVMIK PDGVSRQLVG KIVSRFEERG YKLVAIKSLT PSDALAKEHY ADLSARPFYP SLVKYITSGT PVVAMVWEGK DVIRQGRRIV GATNPLDADA GSVRGQYAVS VGRNLIHASD AFESATKEIG LWFAPEELSE YEPIAWPWVM ADN
NT Sequence
atgttcgcca actctctcag acacggtctt cgcaccgctt ctcgctcctc tgcccgcgca ttctctacca tccccgctcg tgcgcccagg accaacgtcg ttggcgcgct tgccctcggt actgccgtca ctggttatgc cctttacgag tctaccaggt cccctgtcct gcttgagggc cccaggacta ttgccggtga gaagggtacc gttaccgaga ggtcttttgt catgatcaag ccggatggtg tctccaggca actcgttggc aagatcgtct cccgctttga ggagcgtggt tacaagctcg tcgccatcaa atccctcacc ccctctgacg ccctcgccaa agaacactat gccgacctct ctgctcgtcc gttctacccc tcgctcgtca agtacatcac ctctggtact cctgttgtgg cgatggtttg ggagggcaag gatgtcattc gtcagggtcg ccgaatcgta ggcgccacca accccctcga cgccgatgcc ggctccgtcc gcggccaata cgccgtctcc gtcggtcgta acttgatcca cgcctcggac gcctttgagt ctgccaccaa ggagattgga ctttggtttg cgcccgaaga gttgtccgag tatgagccta ttgcttggcc ttgggtcatg gcggacaact ag
Details for CrneC.01218.a.B1.GE41321
HARVESTED ON: 3/31/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 25kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMF ANSLRHGLRT ASRSSARAFS TIPARAPRTN VVGALALGTA VTGYALYEST RSPVLLEGPR TIAGEKGTVT ERSFVMIKPD GVSRQLVGKI VSRFEERGYK LVAIKSLTPS DALAKEHYAD LSARPFYPSL VKYITSGTPV VAMVWEGKDV IRQGRRIVGA TNPLDADAGS VRGQYAVSVG RNLIHASDAF ESATKEIGLW FAPEELSEYE PIAWPWVMAD N
Validated NT Sequence
atcttactca cttagttgtc cgccatgacc caaggccaag caataggctc atactcggac aactcttcgg gcgcaaacca aagtccaatc tccttggtgg cagactcaaa ggcgtccgag gcgtggatca agttacgacc gacggagacg gcgtattggc cgcggacgga gccggcatcg gcgtcgaggg ggttggtggc gcctacgatt cggcgaccct gacgaatgac atccttgccc tcccaaacca tcgccacaac aggagtacca gaggtgatgt acttgacgag cgaggggtag aacggacgag cagagaggtc ggcatagtgt tctttggcga gggcgtcaga gggggtgagg gatttgatgg cgacgagctt gtaaccacgc tcctcaaagc gggagacgat cttgccaacg agttgcctgg agacaccatc cggcttgatc atgacaaaag acctctcggt aacggtaccc ttctcaccgg caatagtcct ggggccctca agcaggacag gggacctggt agactcgtaa agggcataac cagtgacggc agtaccgagg gcaagcgcgc caacgacgtt ggtcctgggc gcacgagcgg ggatggtaga gaatgcgcgg gcagaggagc gagaagcggt gcgaagaccg tgtctgagag agttggcgaa catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMF ANSLRHGLRT ASRSSARAFS TIPARAPRTN VVGALALGTA VTGYALYEST RSPVLLEGPR TIAGEKGTVT ERSFVMIKPD GVSRQLVGKI VSRFEERGYK LVAIKSLTPS DALAKEHYAD LSARPFYPSL VKYITSGTPV VAMVWEGKDV IRQGRRIVGA TNPLDADAGS VRGQYAVSVG RNLIHASDAF ESATKEIGLW FAPEELSEYE PIAWPWVMAD N
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttcg ccaactctct cagacacggt cttcgcaccg cttctcgctc ctctgcccgc gcattctcta ccatccccgc tcgtgcgccc aggaccaacg tcgttggcgc gcttgccctc ggtactgccg tcactggtta tgccctttac gagtctacca ggtcccctgt cctgcttgag ggccccagga ctattgccgg tgagaagggt accgttaccg agaggtcttt tgtcatgatc aagccggatg gtgtctccag gcaactcgtt ggcaagatcg tctcccgctt tgaggagcgt ggttacaagc tcgtcgccat caaatccctc accccctctg acgccctcgc caaagaacac tatgccgacc tctctgctcg tccgttctac ccctcgctcg tcaagtacat cacctctggt actcctgttg tggcgatggt ttgggagggc aaggatgtca ttcgtcaggg tcgccgaatc gtaggcgcca ccaaccccct cgacgccgat gccggctccg tccgcggcca atacgccgtc tccgtcggtc gtaacttgat ccacgcctcg gacgcctttg agtctgccac caaggagatt ggactttggt ttgcgcccga agagttgtcc gagtatgagc ctattgcttg gccttgggtc atggcggaca actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for CrneC.01218.a.B2.GE41322
HARVESTED ON: 3/31/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 18kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TVTERSFVMI KPDGVSRQLV GKIVSRFEER GYKLVAIKSL TPSDALAKEH YADLSARPFY PSLVKYITSG TPVVAMVWEG KDVIRQGRRI VGATNPLDAD AGSVRGQYAV SVGRNLIHAS DAFESATKEI GLWFAPEELS EYEPIAWPWV MADN
Validated NT Sequence
atcttactca cttagttgtc cgccatgacc caaggccaag caataggctc atactcggac aactcttcgg gcgcaaacca aagtccaatc tccttggtgg cagactcaaa ggcgtccgag gcgtggatca agttacgacc gacggagacg gcgtattggc cgcggacgga gccggcatcg gcgtcgaggg ggttggtggc gcctacgatt cggcgaccct gacgaatgac atccttgccc tcccaaacca tcgccacaac aggagtacca gaggtgatgt acttgacgag cgaggggtag aacggacgag cagagaggtc ggcatagtgt tctttggcga gggcgtcaga gggggtgagg gatttgatgg cgacgagctt gtaaccacgc tcctcaaagc gggagacgat cttgccaacg agttgcctgg agacaccatc cggcttgatc atgacaaaag acctctcggt aacggtaccc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHGT VTERSFVMIK PDGVSRQLVG KIVSRFEERG YKLVAIKSLT PSDALAKEHY ADLSARPFYP SLVKYITSGT PVVAMVWEGK DVIRQGRRIV GATNPLDADA GSVRGQYAVS VGRNLIHASD AFESATKEIG LWFAPEELSE YEPIAWPWVM ADN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggtaccg ttaccgagag gtcttttgtc atgatcaagc cggatggtgt ctccaggcaa ctcgttggca agatcgtctc ccgctttgag gagcgtggtt acaagctcgt cgccatcaaa tccctcaccc cctctgacgc cctcgccaaa gaacactatg ccgacctctc tgctcgtccg ttctacccct cgctcgtcaa gtacatcacc tctggtactc ctgttgtggc gatggtttgg gagggcaagg atgtcattcg tcagggtcgc cgaatcgtag gcgccaccaa ccccctcgac gccgatgccg gctccgtccg cggccaatac gccgtctccg tcggtcgtaa cttgatccac gcctcggacg cctttgagtc tgccaccaag gagattggac tttggtttgc gcccgaagag ttgtccgagt atgagcctat tgcttggcct tgggtcatgg cggacaactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat