CrneC.01209.a

CAMKK/CAMKK-META protein kinase

CENTER ID: CrneC.01209.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.01209.a.B2.GE41320 Truncation 1 141 480
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_05484
UniProt: J9VZR1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRDSPSSLP PTLLEPPPPA TIISPPSPTK DKTITKDPSQ PDMISSQASP TIESPDSLPQ SSTGSPPPMS TPLPAPASPA FLRPTSPPFA SSSVSITVRS PSPSSPSFRP KGPLHRRTSS AHRVRETIDG TQTANEDGER MINQYKIGMS LGQGAYAKVE LGVDINTGVK YAIKEFSKSR LHHQSLQEKH RATMRSKLRE GRARKAMSGD ERESRERPSQ PMEKEGMSGT LGGTQPGDEK MEDPLGLIRR EIAVMKKLDH PNLVHLYEAI SVSTADALFL VLEYMPGGTL MKVKIGEDDS NAQPPFDREQ TREYFRQLCL GLEYLHANEV VHRDIKPENI LLSADRQLVK LCDFGVSEMF TKTGDDRIQK SGGSPAFQSP ESFQPNGELH GKAVDIWALG VTLYCMLTGT LPFNYPNIIE LYAALMEKSP RIPEDWDASL RDLIERMLCK DPALRIDMSS LREHPWTTDE TRLPMIDREE NLFEVGKQVE EPTQDEIKDA IVTFRGILYV MRAVHKMRRL HLRRSSPSRG ATSSPGGSAN ASFASESMDS YVSHDRLTST TSLSSDGEDG QYKDIAGNKV MSPRKMSLAS PADTVRPDAT PPMSFGNIEP IKTDVQENVD GVVLVDSPTS ENEDAGTEKL GSGSSRDLQL
NT Sequence
atggatcgcg acagcccttc ctcgctcccc ccgactctcc tcgagcctcc acccccagcc accattatct cacccccctc tccgacaaaa gataagacaa tcacaaaaga tccttctcaa ccagacatga tttcatccca agcatcccct actatcgaat ctccagattc tcttcctcag tccagtactg gctctcctcc tcctatgtcg acacctctac ccgctccggc ttcgcctgcc ttcctccgcc ccacatctcc cccttttgct tcgtcctcag tttccatcac cgttcgatct ccttccccat catcaccctc gttcaggccc aaagggccgc ttcatcgcag aacaagttca gcccatcgtg tccgtgaaac catcgatggc acccaaacag ctaacgaaga cggcgagcgg atgatcaacc agtacaaaat tgggatgagc cttgggcaag gagcatacgc taaagtcgag ctaggagttg atataaacac tggcgtgaaa tatgcaatca aagagttttc aaaatccagg ctccatcacc aatctcttca agagaagcat cgtgcaacta tgcgaagtaa attacgggaa ggaagagcgc gaaaggcaat gagtggggat gaaagggaaa gtcgagaacg gccgtcacag cccatggaaa aggaggggat gtccggcact ttgggcggca cacaacctgg agatgagaag atggaagacc cactggggct gattcgaagg gaaattgctg tgatgaaaaa gctcgatcat cccaatcttg tgcatctgta tgaggccata tcagtttcta ccgccgatgc cctatttttg gtgttggaat acatgcccgg gggaaccctc atgaaagtca aaattgggga agatgactca aatgcccagc cgccttttga tcgcgaacag actagggaat acttcagaca actttgtctt ggcttggaat acctgcatgc taatgaagtc gtacaccgtg atatcaaacc agagaatata ctcctctctg cagataggca gctcgtcaag ctctgtgatt ttggtgtatc agaaatgttc accaagacag gagatgacag gattcaaaaa tctggcggta gcccagcttt tcaaagtcct gaaagctttc aaccgaacgg tgaattacat gggaaggcag tagatatctg ggctcttgga gtcactcttt attgcatgtt gaccggcact ttgcccttca attatccaaa tattatcgag ctgtacgctg ctctcatgga gaaaagtcca cgaattccgg aagactggga tgcctcgctt cgggatttaa tagagcgcat gctttgcaaa gatccagcat tgcgtattga catgtcgagt ttacgagaac atccgtggac aacagacgaa acgcgcttac cgatgatcga cagggaagag aacttgttcg aagtcggcaa acaagtagaa gagcctactc aagacgagat caaggacgcg atcgtaacat tcaggggcat cctgtacgtc atgcgggcag tccataagat gcgccgacta catttacgcc gttcatcgcc atcccgaggt gccacttcat cccccggcgg ttcagccaat gcgtcattcg catcagagtc aatggattcc tacgtttctc acgatcgttt aacttccacc acctcactgt cttcagacgg tgaagatggg caatataagg acattgcagg gaataaagtc atgagtccta ggaaaatgag cctggcatct ccagcggata ctgtgagacc tgacgcaacg cctcccatgt cttttgggaa cattgagcct atcaagacgg acgtgcaaga aaatgtggat ggagttgtac ttgttgattc tccgacatcc gagaacgaag acgcagggac tgaaaaatta ggaagcggct caagccgtga tctgcaatta taa
Details for CrneC.01209.a.B2.GE41320
HARVESTED ON: 3/31/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMI NQYKIGMSLG QGAYAKVELG VDINTGVKYA IKEFSKSRLH HQSLQEKHRA TMRSKLREGR ARKAMSGDER ESRERPSQPM EKEGMSGTLG GTQPGDEKME DPLGLIRREI AVMKKLDHPN LVHLYEAISV STADALFLVL EYMPGGTLMK VKIGEDDSNA QPPFDREQTR EYFRQLCLGL EYLHANEVVH RDIKPENILL SADRQLVKLC DFGVSEMFTK TGDDRIQKSG GSPAFQSPES FQPNGELHGK AVDIWALGVT LYCMLTGTLP FNYPNIIELY AALMEKSPRI PEDWDASLRD LIERMLCKDP ALRIDMSSLR EHPWTTDETR
Validated NT Sequence
taagcgcgtt tcgtctgttg tccacggatg ttctcgtaaa ctcgacatgt caatacgcaa tgctggatct ttgcaaagca tgcgctctat taaatcccga agcgaggcat cccagtcttc cggaattcgt ggacttttct ccatgagagc agcgtacagc tcgataatat ttggataatt gaagggcaaa gtgccggtca acatgcaata aagagtgact ccaagagccc agatatctac tgccttccca tgtaattcac cgttcggttg aaagctttca ggactttgaa aagctgggct accgccagat ttttgaatcc tgtcatctcc tgtcttggtg aacatttctg atacaccaaa atcacagagc ttgacgagct gcctatctgc agagaggagt atattctctg gtttgatatc acggtgtacg acttcattag catgcaggta ttccaagcca agacaaagtt gtctgaagta ttccctagtc tgttcgcgat caaaaggcgg ctgggcattt gagtcatctt ccccaatttt gactttcatg agggttcccc cgggcatgta ttccaacacc aaaaataggg catcggcggt agaaactgat atggcctcat acagatgcac aagattggga tgatcgagct ttttcatcac agcaatttcc cttcgaatca gccccagtgg gtcttccatc ttctcatctc caggttgtgt gccgcccaaa gtgccggaca tcccttcctt ttccatgggc tgtgacggcc gttctcgact ttccctttca tccccactca ttgcctttcg cgctcttcct tcccgtaatt tacttcgcat agttgcacga tgcttctctt gaagagattg gtgatggagc ctggattttg aaaactcttt gattgcatat ttcacgccag tgtttatatc aactcctagc tcgactttag cgtatgctcc ttgcccaagg ctcatcccaa ttttgtactg gttgatcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMI NQYKIGMSLG QGAYAKVELG VDINTGVKYA IKEFSKSRLH HQSLQEKHRA TMRSKLREGR ARKAMSGDER ESRERPSQPM EKEGMSGTLG GTQPGDEKME DPLGLIRREI AVMKKLDHPN LVHLYEAISV STADALFLVL EYMPGGTLMK VKIGEDDSNA QPPFDREQTR EYFRQLCLGL EYLHANEVVH RDIKPENILL SADRQLVKLC DFGVSEMFTK TGDDRIQKSG GSPAFQSPES FQPNGELHGK AVDIWALGVT LYCMLTGTLP FNYPNIIELY AALMEKSPRI PEDWDASLRD LIERMLCKDP ALRIDMSSLR EHPWTTDETR LPMIDREE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatca accagtacaa aattgggatg agccttgggc aaggagcata cgctaaagtc gagctaggag ttgatataaa cactggcgtg aaatatgcaa tcaaagagtt ttcaaaatcc aggctccatc accaatctct tcaagagaag catcgtgcaa ctatgcgaag taaattacgg gaaggaagag cgcgaaaggc aatgagtggg gatgaaaggg aaagtcgaga acggccgtca cagcccatgg aaaaggaggg gatgtccggc actttgggcg gcacacaacc tggagatgag aagatggaag acccactggg gctgattcga agggaaattg ctgtgatgaa aaagctcgat catcccaatc ttgtgcatct gtatgaggcc atatcagttt ctaccgccga tgccctattt ttggtgttgg aatacatgcc cgggggaacc ctcatgaaag tcaaaattgg ggaagatgac tcaaatgccc agccgccttt tgatcgcgaa cagactaggg aatacttcag acaactttgt cttggcttgg aatacctgca tgctaatgaa gtcgtacacc gtgatatcaa accagagaat atactcctct ctgcagatag gcagctcgtc aagctctgtg attttggtgt atcagaaatg ttcaccaaga caggagatga caggattcaa aaatctggcg gtagcccagc ttttcaaagt cctgaaagct ttcaaccgaa cggtgaatta catgggaagg cagtagatat ctgggctctt ggagtcactc tttattgcat gttgaccggc actttgccct tcaattatcc aaatattatc gagctgtacg ctgctctcat ggagaaaagt ccacgaattc cggaagactg ggatgcctcg cttcgggatt taatagagcg catgctttgc aaagatccag cattgcgtat tgacatgtcg agtttacgag aacatccgtg gacaacagac gaaacgcgct taccgatgat cgacagggaa gagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat