SSGCID: Target Page

CrneC.01161.a

Pyruvate kinase (EC 2.7.1.40)

CENTER ID:	CrneC.01161.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.01161.a.B11.GE41724	full length		1	529

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.01161.a.B11.PW38400	full length		1	529

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_01820
UniProt:	T2BNJ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAGTPSSQLA WLSGLSTNFN EMSADQKFLR KTSIIATIGP KTNNVDTLVQ LADAGMNIVR MNFSHGSYEY HQSVIDNARA AAAKSPSGRP IAIALDTKGP EIRTGLMKDD TDVPISAGHE FWVTTDKAYA EAGTAEQIYI DYTNIVKVTA PGKLIYVDDG ILSLQVISIQ GEKIRVKSLN SGVLSSRKGV NLPKTAVDLP ALSEKDKSDL AFGVKNSVDM IFASFIRSAN DVKEIRKVLG PEGADIKIIV KIENEQGVMN FDEILRETDG VMVARGDLGI EIPASQVFMA QKMMIAKCNV AGKPVICATQ MLESMTYNPR PTRAEVSDVA NAVIDGADCV MLSGETAKGK YPIEAVKMMA ETAFLAESSI AYPPLFDQLR ALTPRPTETA ETLALSAVAA AIEQDAGAII VLSTSGVSAR LISKYRPACP IICVTRNEQT ARQLHLSRGV YPVWFPEPRG IPAEKWQIDV DNRIRYGLRM ALGLGIIKPE ATVMAVQGWK GGLGHTNTLR ILSVPSDPAD LDLHSIERD

NT Sequence

atggccggta ccccttcttc ccagctcgct tggctctctg gtctcagcac caacttcaat gagatgtccg ctgatcagaa gttcctccgc aagacttcca tcattgctac cattggcccc aaaaccaaca atgtcgacac tctcgtgcag ctcgctgatg cgggcatgaa catcgttcga atgaactttt ctcatggctc ctatgaatac caccagtctg tcattgacaa tgctcgcgct gctgctgcca agagcccttc cggtcgaccc attgccatcg ctctcgacac caagggtccg gagattagga ctggtttgat gaaggatgac accgatgttc ctatttctgc tggacacgaa ttctgggtta ctaccgacaa ggcttatgca gaggctggta ctgccgagca gatctatatt gactatacca acatcgtcaa ggttactgcc ccgggcaagc tcatctacgt tgacgatggt attctttctc tccaagtcat ctctattcaa ggtgaaaaga tccgtgtcaa gtctctcaac tccggcgttc tctcctctcg caagggtgtc aacctgccca agaccgctgt ggatctcccc gctctttctg aaaaggacaa gtctgatctt gcctttggtg tcaagaacag tgttgacatg atcttcgctt ctttcatccg ttctgccaac gatgtcaagg agatccgtaa ggtcctcggc cctgaaggcg ccgacatcaa gatcattgtc aagattgaga atgagcaggg agtgatgaac ttcgatgaga ttcttaggga aactgacggt gtcatggttg cccgaggtga tttgggtatt gagatccccg caagtcaggt attcatggcc caaaagatga tgattgccaa gtgcaacgtc gctggcaagc ctgtcatctg tgctactcag atgcttgagt ccatgactta caaccctcga cccactcgtg cggaagtttc cgatgttgcc aacgcggtca tcgacggtgc tgactgtgtc atgctttccg gcgagactgc caagggcaag tatcccattg aagctgtcaa gatgatggct gagactgctt tccttgctga gtcttctatc gcctaccctc ctcttttcga ccagctccga gctcttacac ctcgacctac cgagactgcc gagacccttg ctctttctgc tgttgccgct gctatcgagc aggatgccgg cgctatcatt gtgctttcaa cttccggtgt ttctgccaga ttgatttcca agtacaggcc tgcttgtccc atcatctgtg tgacccgaaa cgaacaaacc gctcgacaac tccacctctc gcgaggtgtt taccctgtct ggttccctga gccccgcggt attcctgctg agaagtggca gattgatgtt gacaacagga ttcgatatgg tctcaggatg gctctcggtt tgggcatcat taagcctgag gctaccgtca tggctgtcca aggatggaag ggcggtcttg gccacactaa cactcttcgt atcctctccg ttccttctga ccctgctgac ctcgaccttc actctatcga gcgcgactaa

Details for CrneC.01161.a.B11.GE41724

HARVESTED ON:	6/19/2017
SEQUENCED ON:	6/22/2017
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA GTPSSQLAWL SGLSTNFNEM SADQKFLRKT SIIATIGPKT NNVDTLVQLA DAGMNIVRMN FSHGSYEYHQ SVIDNARAAA AKSPSGRPIA IALDTKGPEI RTGLMKDDTD VPISAGHEFW VTTDKAYAEA GTAEQIYIDY TNIVKVTAPG KLIYVDDGIL SLQVISIQGE KIRVKSLNSG VLSSRKGVNL PKTAVDLPAL SEKDKSDLAF GVKNSVDMIF ASFIRSANDV KEIRKVLGPE GADIKIIVKI ENEQGVMNFD EILRETDGVM VARGDLGIEI PASQVFMAQK MMIAKCNVAG KPVICATQML ESMTYNPRPT RAEVSDVANA VIDGADCVML SGETAKGKYP IEAVKMMAET AFLAESSIAY PPLFDQLRAL TPRPTETAET LALSAVAAAI EQDAGAIIVL STSGVSARLI SKYRPACPII CVTRNEQTAR QLHLSRGVYP VWFPEPRGIP AEKWQIDVDN RIRYGLRMAL GLGIIKPEAT VMAVQGWKGG LGHTNTLRIL SVPSDPADLD LHSIERD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccg gtaccccttc ttcccagctc gcttggctct ctggtctcag caccaacttc aatgagatgt ccgctgatca gaagttcctc cgcaagactt ccatcattgc taccattggc cccaaaacca acaatgtcga cactctcgtg cagctcgctg atgcgggcat gaacatcgtt cgaatgaact tttctcatgg ctcctatgaa taccaccagt ctgtcattga caatgctcgc gctgctgctg ccaagagccc ttccggtcga cccattgcca tcgctctcga caccaagggt ccggagatta ggactggttt gatgaaggat gacaccgatg ttcctatttc tgctggacac gaattctggg ttactaccga caaggcttat gcagaggctg gtactgccga gcagatctat attgactata ccaacatcgt caaggttact gccccgggca agctcatcta cgttgacgat ggtattcttt ctctccaagt catctctatt caaggtgaaa agatccgtgt caagtctctc aactccggcg ttctctcctc tcgcaagggt gtcaacctgc ccaagaccgc tgtggatctc cccgctcttt ctgaaaagga caagtctgat cttgcctttg gtgtcaagaa cagtgttgac atgatcttcg cttctttcat ccgttctgcc aacgatgtca aggagatccg taaggtcctc ggccctgaag gcgccgacat caagatcatt gtcaagattg agaatgagca gggagtgatg aacttcgatg agattcttag ggaaactgac ggtgtcatgg ttgcccgagg tgatttgggt attgagatcc ccgcaagtca ggtattcatg gcccaaaaga tgatgattgc caagtgcaac gtcgctggca agcctgtcat ctgtgctact cagatgcttg agtccatgac ttacaaccct cgacccactc gtgcggaagt ttccgatgtt gccaacgcgg tcatcgacgg tgctgactgt gtcatgcttt ccggcgagac tgccaagggc aagtatccca ttgaagctgt caagatgatg gctgagactg ctttccttgc tgagtcttct atcgcctacc ctcctctttt cgaccagctc cgagctctta cacctcgacc taccgagact gccgagaccc ttgctctttc tgctgttgcc gctgctatcg agcaggatgc cggcgctatc attgtgcttt caacttccgg tgtttctgcc agattgattt ccaagtacag gcctgcttgt cccatcatct gtgtgacccg aaacgaacaa accgctcgac aactccacct ctcgcgaggt gtttaccctg tctggttccc tgagccccgc ggtattcctg ctgagaagtg gcagattgat gttgacaaca ggattcgata tggtctcagg atggctctcg gtttgggcat cattaagcct gaggctaccg tcatggctgt ccaaggatgg aagggcggtc ttggccacac taacactctt cgtatcctct ccgttccttc tgaccctgct gacctcgacc ttcactctat cgagcgcgac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for CrneC.01161.a.B11.PW38400

PURIFICATION DATe:	12/4/2017
CONCENTRATION:	9.58mg/ml
OBSERVED MW:	60kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

Started to crash during concentration

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCG GTACCCCTTC TTCCCAGCTC GCTTGGCTCT CTGGTCTCAG CACCAACTTC AATGAGATGT CCGCTGATCA GAAGTTCCTC CGCAAGACTT CCATCATTGC TACCATTGGC CCCAAAACCA ACAATGTCGA CACTCTCGTG CAGCTCGCTG ATGCGGGCAT GAACATCGTT CGAATGAACT TTTCTCATGG CTCCTATGAA TACCACCAGT CTGTCATTGA CAATGCTCGC GCTGCTGCTG CCAAGAGCCC TTCCGGTCGA CCCATTGCCA TCGCTCTCGA CACCAAGGGT CCGGAGATTA GGACTGGTTT GATGAAGGAT GACACCGATG TTCCTATTTC TGCTGGACAC GAATTCTGGG TTACTACCGA CAAGGCTTAT GCAGAGGCTG GTACTGCCGA GCAGATCTAT ATTGACTATA CCAACATCGT CAAGGTTACT GCCCCGGGCA AGCTCATCTA CGTTGACGAT GGTATTCTTT CTCTCCAAGT CATCTCTATT CAAGGTGAAA AGATCCGTGT CAAGTCTCTC AACTCCGGCG TTCTCTCCTC TCGCAAGGGT GTCAACCTGC CCAAGACCGC TGTGGATCTC CCCGCTCTTT CTGAAAAGGA CAAGTCTGAT CTTGCCTTTG GTGTCAAGAA CAGTGTTGAC ATGATCTTCG CTTCTTTCAT CCGTTCTGCC AACGATGTCA AGGAGATCCG TAAGGTCCTC GGCCCTGAAG GCGCCGACAT CAAGATCATT GTCAAGATTG AGAATGAGCA GGGAGTGATG AACTTCGATG AGATTCTTAG GGAAACTGAC GGTGTCATGG TTGCCCGAGG TGATTTGGGT ATTGAGATCC CCGCAAGTCA GGTATTCATG GCCCAAAAGA TGATGATTGC CAAGTGCAAC GTCGCTGGCA AGCCTGTCAT CTGTGCTACT CAGATGCTTG AGTCCATGAC TTACAACCCT CGACCCACTC GTGCGGAAGT TTCCGATGTT GCCAACGCGG TCATCGACGG TGCTGACTGT GTCATGCTTT CCGGCGAGAC TGCCAAGGGC AAGTATCCCA TTGAAGCTGT CAAGATGATG GCTGAGACTG CTTTCCTTGC TGAGTCTTCT ATCGCCTACC CTCCTCTTTT CGACCAGCTC CGAGCTCTTA CACCTCGACC TACCGAGACT GCCGAGACCC TTGCTCTTTC TGCTGTTGCC GCTGCTATCG AGCAGGATGC CGGCGCTATC ATTGTGCTTT CAACTTCCGG TGTTTCTGCC AGATTGATTT CCAAGTACAG GCCTGCTTGT CCCATCATCT GTGTGACCCG AAACGAACAA ACCGCTCGAC AACTCCACCT CTCGCGAGGT GTTTACCCTG TCTGGTTCCC TGAGCCCCGC GGTATTCCTG CTGAGAAGTG GCAGATTGAT GTTGACAACA GGATTCGATA TGGTCTCAGG ATGGCTCTCG GTTTGGGCAT CATTAAGCCT GAGGCTACCG TCATGGCTGT CCAAGGATGG AAGGGCGGTC TTGGCCACAC TAACACTCTT CGTATCCTCT CCGTTCCTTC TGACCCTGCT GACCTCGACC TTCACTCTAT CGAGCGCGAC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT