CrneC.01141.a
ATP-binding protein (ribokinase)
| CENTER ID: | CrneC.01141.a |
| ORGANISM: | Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487) |
| ASSOCIATED DISEASE: | Pneumonia-like illness |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrneC.01141.a.B12.GE41811 | full length | 1 | 324 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrneC.01141.a.B12.PW38396 | full length | 1 | 324 |
Structures
6CW5
DEPOSITED: 3/29/2018
DETERMINATION: XRay
CLONE: CrneC.01141.a.B12.GE41811
PROTEIN: CrneC.01141.a.B12.PW38396
6XK2
DEPOSITED: 6/25/2020
DETERMINATION: XRay
CLONE: CrneC.01141.a.B12.GE41811
PROTEIN: CrneC.01141.a.B12.PW38396
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:CNAG_02296 |
| UniProt: | J9VQ51 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTSSRCLVR GSVNIDEFFH LPHIVRPGET ISSTGLTKRA GGKGANQAFA VARAGGQVEL DGAIGDDGIW VKEMLESAGV GTDKLKIVKD EVTGRAVIQS AADGENSIVL HAGANYYLPS PTPTTSLATY THLLVQNEVP LSSTLAYLTA AGQSSPPLTS VFNPSPMLTP AQLREFPWKH LSWLIVNEGE LGDLLLAFGS SANPGEAKED ELQAKASAGI LELHENDYFS KNVGIICTLG AKGILCYEPG KEVGYLPAAK LQNPVKDTTG AGDCFAGYFV AGLMSGKSLQ DALKTCLVAC GICVENEGAM ESVPTLNAVK ERLA
NT Sequence
atgtccacta gttcccgttg cctcgtccga ggatcagtca acattgacga attcttccac cttccgcaca tcgtccgtcc tggagagacc atctcctcta caggtcttac aaagcgagca gggggtaaag gtgccaatca agcgttcgct gtcgcccgtg caggcgggca ggttgagctt gacggtgcta taggagacga cggcatatgg gtcaaggaga tgcttgagag tgctggtgtg gggacagaca agctaaagat tgtcaaggat gaggttacag ggagggccgt tattcagagt gctgcagatg gagagaactc tatagttctt catgccggtg ccaactacta ccttccctct cctactccca ccacttccct cgctacatat acccatctcc tcgttcaaaa tgaagttcct ctctcttcca cactcgccta cctcaccgca gcgggtcaat cctctccccc tttgaccagt gttttcaacc catcaccgat gctcaccccg gctcaacttc gagaattccc ttggaagcac ctttcatggc tcattgttaa cgaaggggaa ttgggagacc ttttattggc gtttgggtca tcagcgaacc ccggtgaagc caaggaagat gaacttcaag ccaaagcttc tgctggaatt ctcgaattgc atgagaacga ctatttttcc aaaaacgtcg gtattatctg tactcttgga gcgaagggaa tactttgcta cgaacccgga aaggaagttg gctacctgcc agctgcgaag ttgcaaaacc cagtgaagga tactaccggg gccggtgact gttttgctgg ctactttgtg gctggcttga tgagtggaaa gagcctacag gatgctttga aaacttgtct tgtggcttgt ggcatctgcg tggagaatga gggtgctatg gaaagtgtcc ccactctcaa cgcagtcaaa gagcgtcttg cttag
Details for CrneC.01141.a.B12.GE41811
| HARVESTED ON: | 8/7/2017 |
| SEQUENCED ON: | 9/20/2017 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMS TSSRCLVRGS VNIDEFFHLP HIVRPGETIS STGLTKRAGG KGANQAFAVA RAGGQVELDG AIGDDGIWVK EMLESAGVGT DKLKIVKDEV TGRAVIQSAA DGENSIVLHA GANYYLPSPT PTTSLATYTH LLVQNEVPLS STLAYLTAAG QSSPPLTSVF NPSPMLTPAQ LREFPWKHLS WLIVNEGELG DLLLAFGSSA NPGEAKEDEL QAKASAGILE LHENDYFSKN VGIICTLGAK GILCYEPGKE VGYLPAAKLQ NPVKDTTGAG DCFAGYFVAG LMSGKSLQDA LKTCLVACGI CVENEGAMES VPT
Validated NT Sequence
gagtggggac actttccata gcaccctcat tctccacgca gatgccacaa gccacaagac aagttttcaa agcatcctgt aggctctttc cactcatcaa gccagccaca aagtagccag caaaacagtc accggccccg gtagtatcct tcactgggtt ttgcaacttc gcagctggca ggtagccaac ttcctttccg ggttcgtagc aaagtattcc cttcgctcca agagtacaga taataccgac gtttttggaa aaatagtcgt tctcatgcaa ttcgagaatt ccagcagaag ctttggcttg aagttcatct tccttggctt caccggggtt cgctgatgac ccaaacgcca ataaaaggtc tcccaattcc ccttcgttaa caatgagcca tgaaaggtgc ttccaaggga attctcgaag ttgagccggg gtgagcatcg gtgatgggtt gaaaacactg gtcaaagggg gagaggattg acccgctgcg gtgaggtagg cgagtgtgga agagagagga acttcatttt gaacgaggag atgggtatat gtagcgaggg aagtggtggg agtaggagag ggaaggtagt agttggcacc ggcatgaaga actatagagt tctctccatc tgcagcactc tgaataacgg ccctccctgt aacctcatcc ttgacaatct ttagcttgtc tgtccccaca ccagcactct caagcatctc cttgacccat atgccgtcgt ctcctatagc accgtcaagc tcaacctgcc cgcctgcacg ggcgacagcg aacgcttgat tggcaccttt accccctgct cgctttgtaa gacctgtaga ggagatggtc tctccaggac ggacgatgtg cggaaggtgg aagaattcgt caatgttgac tgatcctcgg acgaggcaac gggaactagt ggacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMS TSSRCLVRGS VNIDEFFHLP HIVRPGETIS STGLTKRAGG KGANQAFAVA RAGGQVELDG AIGDDGIWVK EMLESAGVGT DKLKIVKDEV TGRAVIQSAA DGENSIVLHA GANYYLPSPT PTTSLATYTH LLVQNEVPLS STLAYLTAAG QSSPPLTSVF NPSPMLTPAQ LREFPWKHLS WLIVNEGELG DLLLAFGSSA NPGEAKEDEL QAKASAGILE LHENDYFSKN VGIICTLGAK GILCYEPGKE VGYLPAAKLQ NPVKDTTGAG DCFAGYFVAG LMSGKSLQDA LKTCLVACGI CVENEGAMES VPTLNAVKER LA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca ctagttcccg ttgcctcgtc cgaggatcag tcaacattga cgaattcttc caccttccgc acatcgtccg tcctggagag accatctcct ctacaggtct tacaaagcga gcagggggta aaggtgccaa tcaagcgttc gctgtcgccc gtgcaggcgg gcaggttgag cttgacggtg ctataggaga cgacggcata tgggtcaagg agatgcttga gagtgctggt gtggggacag acaagctaaa gattgtcaag gatgaggtta cagggagggc cgttattcag agtgctgcag atggagagaa ctctatagtt cttcatgccg gtgccaacta ctaccttccc tctcctactc ccaccacttc cctcgctaca tatacccatc tcctcgttca aaatgaagtt cctctctctt ccacactcgc ctacctcacc gcagcgggtc aatcctctcc ccctttgacc agtgttttca acccatcacc gatgctcacc ccggctcaac ttcgagaatt cccttggaag cacctttcat ggctcattgt taacgaaggg gaattgggag accttttatt ggcgtttggg tcatcagcga accccggtga agccaaggaa gatgaacttc aagccaaagc ttctgctgga attctcgaat tgcatgagaa cgactatttt tccaaaaacg tcggtattat ctgtactctt ggagcgaagg gaatactttg ctacgaaccc ggaaaggaag ttggctacct gccagctgcg aagttgcaaa acccagtgaa ggatactacc ggggccggtg actgttttgc tggctacttt gtggctggct tgatgagtgg aaagagccta caggatgctt tgaaaacttg tcttgtggct tgtggcatct gcgtggagaa tgagggtgct atggaaagtg tccccactct caacgcagtc aaagagcgtc ttgcttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for CrneC.01141.a.B12.PW38396
| PURIFICATION DATe: | 11/27/2017 |
| CONCENTRATION: | 19.46mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS TSSRCLVRGS VNIDEFFHLP HIVRPGETIS STGLTKRAGG KGANQAFAVA RAGGQVELDG AIGDDGIWVK EMLESAGVGT DKLKIVKDEV TGRAVIQSAA DGENSIVLHA GANYYLPSPT PTTSLATYTH LLVQNEVPLS STLAYLTAAG QSSPPLTSVF NPSPMLTPAQ LREFPWKHLS WLIVNEGELG DLLLAFGSSA NPGEAKEDEL QAKASAGILE LHENDYFSKN VGIICTLGAK GILCYEPGKE VGYLPAAKLQ NPVKDTTGAG DCFAGYFVAG LMSGKSLQDA LKTCLVACGI CVENEGAMES VPT
Validated NT Sequence
gagtggggac actttccata gcaccctcat tctccacgca gatgccacaa gccacaagac aagttttcaa agcatcctgt aggctctttc cactcatcaa gccagccaca aagtagccag caaaacagtc accggccccg gtagtatcct tcactgggtt ttgcaacttc gcagctggca ggtagccaac ttcctttccg ggttcgtagc aaagtattcc cttcgctcca agagtacaga taataccgac gtttttggaa aaatagtcgt tctcatgcaa ttcgagaatt ccagcagaag ctttggcttg aagttcatct tccttggctt caccggggtt cgctgatgac ccaaacgcca ataaaaggtc tcccaattcc ccttcgttaa caatgagcca tgaaaggtgc ttccaaggga attctcgaag ttgagccggg gtgagcatcg gtgatgggtt gaaaacactg gtcaaagggg gagaggattg acccgctgcg gtgaggtagg cgagtgtgga agagagagga acttcatttt gaacgaggag atgggtatat gtagcgaggg aagtggtggg agtaggagag ggaaggtagt agttggcacc ggcatgaaga actatagagt tctctccatc tgcagcactc tgaataacgg ccctccctgt aacctcatcc ttgacaatct ttagcttgtc tgtccccaca ccagcactct caagcatctc cttgacccat atgccgtcgt ctcctatagc accgtcaagc tcaacctgcc cgcctgcacg ggcgacagcg aacgcttgat tggcaccttt accccctgct cgctttgtaa gacctgtaga ggagatggtc tctccaggac ggacgatgtg cggaaggtgg aagaattcgt caatgttgac tgatcctcgg acgaggcaac gggaactagt ggacatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMS TSSRCLVRGS VNIDEFFHLP HIVRPGETIS STGLTKRAGG KGANQAFAVA RAGGQVELDG AIGDDGIWVK EMLESAGVGT DKLKIVKDEV TGRAVIQSAA DGENSIVLHA GANYYLPSPT PTTSLATYTH LLVQNEVPLS STLAYLTAAG QSSPPLTSVF NPSPMLTPAQ LREFPWKHLS WLIVNEGELG DLLLAFGSSA NPGEAKEDEL QAKASAGILE LHENDYFSKN VGIICTLGAK GILCYEPGKE VGYLPAAKLQ NPVKDTTGAG DCFAGYFVAG LMSGKSLQDA LKTCLVACGI CVENEGAMES VPTLNAVKER LA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCA CTAGTTCCCG TTGCCTCGTC CGAGGATCAG TCAACATTGA CGAATTCTTC CACCTTCCGC ACATCGTCCG TCCTGGAGAG ACCATCTCCT CTACAGGTCT TACAAAGCGA GCAGGGGGTA AAGGTGCCAA TCAAGCGTTC GCTGTCGCCC GTGCAGGCGG GCAGGTTGAG CTTGACGGTG CTATAGGAGA CGACGGCATA TGGGTCAAGG AGATGCTTGA GAGTGCTGGT GTGGGGACAG ACAAGCTAAA GATTGTCAAG GATGAGGTTA CAGGGAGGGC CGTTATTCAG AGTGCTGCAG ATGGAGAGAA CTCTATAGTT CTTCATGCCG GTGCCAACTA CTACCTTCCC TCTCCTACTC CCACCACTTC CCTCGCTACA TATACCCATC TCCTCGTTCA AAATGAAGTT CCTCTCTCTT CCACACTCGC CTACCTCACC GCAGCGGGTC AATCCTCTCC CCCTTTGACC AGTGTTTTCA ACCCATCACC GATGCTCACC CCGGCTCAAC TTCGAGAATT CCCTTGGAAG CACCTTTCAT GGCTCATTGT TAACGAAGGG GAATTGGGAG ACCTTTTATT GGCGTTTGGG TCATCAGCGA ACCCCGGTGA AGCCAAGGAA GATGAACTTC AAGCCAAAGC TTCTGCTGGA ATTCTCGAAT TGCATGAGAA CGACTATTTT TCCAAAAACG TCGGTATTAT CTGTACTCTT GGAGCGAAGG GAATACTTTG CTACGAACCC GGAAAGGAAG TTGGCTACCT GCCAGCTGCG AAGTTGCAAA ACCCAGTGAA GGATACTACC GGGGCCGGTG ACTGTTTTGC TGGCTACTTT GTGGCTGGCT TGATGAGTGG AAAGAGCCTA CAGGATGCTT TGAAAACTTG TCTTGTGGCT TGTGGCATCT GCGTGGAGAA TGAGGGTGCT ATGGAAAGTG TCCCCACTCT CAACGCAGTC AAAGAGCGTC TTGCTTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT