CrneC.01045.a

Pyridoxal kinase

CENTER ID: CrneC.01045.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.01045.a.B11.GE41721 full length 1 402
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_05200
UniProt: J9VRZ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTVGQKGMC QDGERILSIQ SHVVSGYVGN RAATFPLQTL GYDVDVINTV QFSNHTGYGF TDGHKTSPDE LAAIFNGMAI NGLLTHPRIL TGYIPSAEAL SVVTDRIRRM KADNPSLIYL LDPVMGDIGT GLYVSRDVVP LYKEMLNLAT IITPNQFEVE LLSGITITSL ETLQNALKKL HIVNQLPHIA FSSIPLPISL VESLSLPAPP PSYTRLLPQP LPPWYDAVGA GAPDDEVLVC FASSWLDGQM ETYAFALPTI RGYFSGVGDL FSAMVLAHFK NPEANPNLSP LRSAVSKALL TVQQILLQTH VHSLAQTEVF GTATPRPLHH PSDPLPADSV IPSDTELDAL KPLNPKDPKR KARRMRLREL RVVQERALIQ DGGEGWPGKR IDWTHISEQL GK
NT Sequence
atgtcgactg tcggacagaa aggaatgtgt caagatggag agaggatact atctatccaa tcgcatgtgg tttcaggcta cgtcggcaat agggctgcca cgtttccact acagactcta ggttatgatg tggatgtgat caacaccgtc cagttctcca atcatacagg ttacggtttc acggatggac ataaaacctc gcctgacgag ttggccgcca tattcaatgg catggctatc aatggactac ttacgcaccc taggatactt acaggttata taccaagtgc tgaggcgctg agtgtcgtca ccgatagaat aaggagaatg aaggcagaca acccctccct tatatatctt cttgaccccg tcatgggaga cattggaacg ggtctgtatg tatccaggga tgtagtccct ctatacaagg aaatgttaaa tttggctacg atcataacac caaaccagtt cgaagtggag cttctatcag gaatcacgat cacttctcta gaaacattac aaaatgccct taaaaagcta cacattgtca atcagctccc ccatattgct ttttcgtcta ttccccttcc catctctctt gtcgagtcac tttccttgcc agctccacct ccctcgtaca cacgtttact tccccaacct cttccaccgt ggtatgatgc tgttggagca ggtgcccctg atgatgaggt tctagtttgc tttgccagca gctggttgga tggtcaaatg gaaacatacg cttttgctct tcccaccatc agaggttatt tctcgggagt tggggacctt ttctcagcca tggttttggc tcatttcaag aatcctgaag ccaaccccaa cctttcacct cttcgctcgg ccgtttcaaa agcgttgttg actgtgcaac agatccttct acaaactcat gtccattcac tcgctcaaac agaagtcttt gggactgcga caccgcggcc actgcaccat ccctctgatc ctttacctgc tgattcagtt ataccttccg atacagagct tgatgctctt aaacctttga atcctaaaga ccccaagaga aaggcccgaa ggatgagatt acgagagtta agagtggtgc aggaaagagc actgattcag gatggcggag aaggatggcc gggaaaaaga attgactgga cacacatatc tgaacaactt ggaaaatag
Details for CrneC.01045.a.B11.GE41721
HARVESTED ON: 6/19/2017
SEQUENCED ON: 6/22/2017
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS TVGQKGMCQD GERILSIQSH VVSGYVGNRA ATFPLQTLGY DVDVINTVQF SNHTGYGFTD GHKTSPDELA AIFNGMAING LLTHPRILTG YIPSAEALSV VTDRIRRMKA DNPSLIYLLD PVMGDIGTGL YVSRDVVPLY KEMLNLATII TPNQFEVELL SGITITSLET LQNALKKLHI VNQLPHIAFS SIPLPISLVE SLSLPAPPPS YTRLLPQPLP PWYDAVGAGA PDDEVLVCFA SSWLDGQMET YAFALPTIRG YFSGVGDLFS AMVLAHFKNP EANPNLSPLR SAVSKALLTV QQILLQTHVH SLAQTEVFGT ATPRPLHHPS DPLPXDSVIP SDTELDALKP LNPKDPKRKA RRMRLRELRV VQERALIQDG GEGWPGKRID WT
Validated NT Sequence
gtgtgtccag tcaattcttt ttcccggcca tccttctccg ccatcctgaa tcagtgctct ttcctgcacc actcttaact ctcgtaatct catccttcgg gcctttctct tggggtcttt aggattcaaa ggtttaagag catcaagctc tgtatcggaa ggtataactg aatcnnnagg taaaggatca gagggatggt gcagtggccg cggtgtcgca gtcccaaaga cttctgtttg agcgagtgaa tggacatgag tttgtagaag gatctgttgc acagtcaaca acgcttttga aacggccgag cgaagaggtg aaaggttggg gttggcttca ggattcttga aatgagccaa aaccatggct gagaaaaggt ccccaactcc cgagaaataa cctctgatgg tgggaagagc aaaagcgtat gtttccattt gaccatccaa ccagctgctg gcaaagcaaa ctagaacctc atcatcaggg gcacctgctc caacagcatc ataccacggt ggaagaggtt ggggaagtaa acgtgtgtac gagggaggtg gagctggcaa ggaaagtgac tcgacaagag agatgggaag gggaatagac gaaaaagcaa tatgggggag ctgattgaca atgtgtagct ttttaagggc attttgtaat gtttctagag aagtgatcgt gattcctgat agaagctcca cttcgaactg gtttggtgtt atgatcgtag ccaaatttaa catttccttg tatagaggga ctacatccct ggatacatac agacccgttc caatgtctcc catgacgggg tcaagaagat atataaggga ggggttgtct gccttcattc tccttattct atcggtgacg acactcagcg cctcagcact tggtatataa cctgtaagta tcctagggtg cgtaagtagt ccattgatag ccatgccatt gaatatggcg gccaactcgt caggcgaggt tttatgtcca tccgtgaaac cgtaacctgt atgattggag aactggacgg tgttgatcac atccacatca taacctagag tctgtagtgg aaacgtggca gccctattgc cgacgtagcc tgaaaccaca tgcgattgga tagatagtat cctctctcca tcttgacaca ttcctttctg tccgacagtc gacatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMS TVGQKGMCQD GERILSIQSH VVSGYVGNRA ATFPLQTLGY DVDVINTVQF SNHTGYGFTD GHKTSPDELA AIFNGMAING LLTHPRILTG YIPSAEALSV VTDRIRRMKA DNPSLIYLLD PVMGDIGTGL YVSRDVVPLY KEMLNLATII TPNQFEVELL SGITITSLET LQNALKKLHI VNQLPHIAFS SIPLPISLVE SLSLPAPPPS YTRLLPQPLP PWYDAVGAGA PDDEVLVCFA SSWLDGQMET YAFALPTIRG YFSGVGDLFS AMVLAHFKNP EANPNLSPLR SAVSKALLTV QQILLQTHVH SLAQTEVFGT ATPRPLHHPS DPLPADSVIP SDTELDALKP LNPKDPKRKA RRMRLRELRV VQERALIQDG GEGWPGKRID WTHISEQLGK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcga ctgtcggaca gaaaggaatg tgtcaagatg gagagaggat actatctatc caatcgcatg tggtttcagg ctacgtcggc aatagggctg ccacgtttcc actacagact ctaggttatg atgtggatgt gatcaacacc gtccagttct ccaatcatac aggttacggt ttcacggatg gacataaaac ctcgcctgac gagttggccg ccatattcaa tggcatggct atcaatggac tacttacgca ccctaggata cttacaggtt atataccaag tgctgaggcg ctgagtgtcg tcaccgatag aataaggaga atgaaggcag acaacccctc ccttatatat cttcttgacc ccgtcatggg agacattgga acgggtctgt atgtatccag ggatgtagtc cctctataca aggaaatgtt aaatttggct acgatcataa caccaaacca gttcgaagtg gagcttctat caggaatcac gatcacttct ctagaaacat tacaaaatgc ccttaaaaag ctacacattg tcaatcagct cccccatatt gctttttcgt ctattcccct tcccatctct cttgtcgagt cactttcctt gccagctcca cctccctcgt acacacgttt acttccccaa cctcttccac cgtggtatga tgctgttgga gcaggtgccc ctgatgatga ggttctagtt tgctttgcca gcagctggtt ggatggtcaa atggaaacat acgcttttgc tcttcccacc atcagaggtt atttctcggg agttggggac cttttctcag ccatggtttt ggctcatttc aagaatcctg aagccaaccc caacctttca cctcttcgct cggccgtttc aaaagcgttg ttgactgtgc aacagatcct tctacaaact catgtccatt cactcgctca aacagaagtc tttgggactg cgacaccgcg gccactgcac catccctctg atcctttacc tgctgattca gttatacctt ccgatacaga gcttgatgct cttaaacctt tgaatcctaa agaccccaag agaaaggccc gaaggatgag attacgagag ttaagagtgg tgcaggaaag agcactgatt caggatggcg gagaaggatg gccgggaaaa agaattgact ggacacacat atctgaacaa cttggaaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat