CrneC.01011.a
Calcineurin subunit B (Calcineurin regulatory subunit) (Protein phosphatase 2B regulatory subunit)
| CENTER ID: | CrneC.01011.a |
| ORGANISM: | Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487) |
| ASSOCIATED DISEASE: | Pneumonia-like illness |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrneC.01011.a.EX11.GE00506 | full length | 2 | 176 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
6TZ8
DEPOSITED: 11/11/2017
DETERMINATION: XRay
CLONE: CrneC.01011.a.EX11.GE00628
PROTEIN: CrneC.01011.a.EX11.PD00467
Publications by SSGCID
Harnessing calcineurin-FK506-FKBP12 crystal structures from invasive fungal pathogens to develop antifungal agents
Fox D, Hoy M, Juvvadi PR, Bobay B, Gobeil S, Venters R, Chang Z, Lin J, Averette A, Cole D, Barrington B, Wheaton J, Ciofani M, Trzoss M, Li X, Lee S, Chen Y, Mutz M, Spicer L, Schumacher M, Heitman J, Steinbach W
Nat Commun - 2019
volume 10, pages 4275
PMID: 31537789; PMCID: PMC6753081
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:CNBD5950 |
| RefSeq: | XP_775641.1 |
| UniProt: | P0CM55 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGAAESSMFN SLEKNSNFSG PELMRLKKRF MKLDKDGSGS IDKDEFLQIP QIANNPLAHR MIAIFDEDGS GTVDFQEFVG GLSAFSSKGG RDEKLRFAFK VYDMDRDGYI SNGELYLVLK QMVGNNLKDQ QLQQIVDKTI MEADKDGDGK LSFEEFTQMV ASTDIVKQMT LEDLF
NT Sequence
atgggtgccg ctgaatcctc catgttcaac tctctggaga agaactccaa cttctcagga ccggagctta tgaggttgaa aaagaggttc atgaagcttg acaaggacgg ttccggatcg attgacaaag acgagtttct tcagatccct caaatcgcga ataacccttt ggcgcatcga atgatagcaa tctttgatga agatggaagt ggaacggttg acttccaaga atttgtcgga ggtttgagcg ctttcagtag taaaggaggt cgtgatgaga agctgagatt cgctttcaag gtgtacgaca tggatcgaga cggctacatc tctaacggtg aactgtatct tgtgttgaag caaatggtcg gaaataacct taaagaccaa caattgcaac aaattgtaga caaaaccatc atggaggctg acaaggacgg ggatggaaag ctctcttttg aggagttcac acaaatggtc gccagcacag atattgtgaa gcaaatgacc cttgaagatc ttttctaa
Details for CrneC.01011.a.EX11.GE00506
| HARVESTED ON: | 12/18/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 20kDa |
| OBSERVED MW: | 20kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Data Unavailable |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MGAAESSMFN SLEKNSNFSG PELMRLKKRF MKLDKDGSGS IDKDEFLQIP QIANNPLAHR MIAIFDEDGS GTVDFQEFVG GLSAFSSKGG RDEKLRFAFK VYDMDRDGYI SNGELYLVLK QMVGNNLKDQ QLQQIVDKTI MEADKDGDGK LSFEEFTQMV ASTDIVKQMT LEDLF
Full NT Sequence (Expression Vector + Insert)
ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatccatg ggtgctgctg agtcttctat gtttaactct ctggagaaga actctaactt ctccggtcct gaactgatgc gtctgaaaaa acgtttcatg aaactggaca aagacggctc cggttctatc gacaaagacg agttcctgca gatcccacag atcgctaaca accctctggc ccaccgcatg attgctatct ttgacgaaga cggctctggt actgtggact ttcaggagtt cgttggtggc ctgtccgctt ttagcagcaa gggcggtcgt gacgagaagc tgcgcttcgc cttcaaagtg tatgacatgg accgcgacgg ttacatttct aacggcgaac tgtacctcgt tctgaaacaa atggttggca acaacctgaa agaccagcag ctgcagcaga ttgttgataa aaccattatg gaagctgaca aagacggcga cggcaaactg tctttcgaag agttcaccca gatggtagct tccactgata tcgtgaagca gatgaccctg gaagacctgt tctgataagg tcaccaccac caccaccact aaaagcttgc ggccgcactc gagcctaggt agctgagcgc atgcaagctg atccgggtta ttagtacatt tattaagcgc tagattctgt gcgttgttga tttacagaca attgttgtac gtattttaat aattcattaa atttataatc tttagggtgg tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt aaatattaaa tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg tttttccgaa ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc caaatcttgt agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa tagagcttgg acatatttaa catcgggcgt gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc gatcaaattt gtagttgagc tttttggaat tatttctgat tgcgggcgtt tttgggcggg tttcaatcta actgtgcccg attttaattc agacaacacg ttagaaagcg atggtgcagg cggtggtaac atttcagacg gcaaatctac taatggcggc ggtggtggag ctgatgataa atctaccatc ggtggaggcg caggcggggc tggcggcgga ggcggaggcg gaggtggtgg cggtgatgca gacggcggtt taggctcaaa tgtctcttta ggcaacacag tcggcacctc aactattgta ctggtttcgg gcgccgtttt tggtttgacc ggtctgagac gagtgcgatt tttttcgttt ctaatagctt ccaacaattg ttgtctgtcg tctaaaggtg cagcgggttg aggttccgtc ggcattggtg gagcgggcgg caattcagac atcgatggtg gtggtggtgg tggaggcgct ggaatgttag gcacgggaga aggtggtggc ggcggtgccg ccggtataat ttgttctggt ttagtttgtt cgcgcacgat tgtgggcacc ggcgcaggcg ccgctggctg cacaacggaa ggtcgtctgc ttcgaggcag cgcttggggt ggtggcaatt caatattata attggaatac aaatcgtaaa aatctgctat aagcattgta atttcgctat cgtttaccgt gccgatattt aacaaccgct caatgtaagc aattgtattg taaagagatt gtctcaagct cggatcgatc ccgcacgccg ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc gctgtcgtcg aggtttaaac gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg cca