SSGCID: Target Page

CrneC.01010.e

CMGC/CDK/CDC2 protein kinase

CENTER ID:	CrneC.01010.e
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.01010.e.B1.GE41262	full length		1	298

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_01664
UniProt:	J9VUC9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLDNYQKIE KVGEGTYGVV YKAKDINTGN IVALKKIRLE AEDEGVPSTS IREISLLKEL SKDDNIVKLL DIVHSEAKLY LVFEFLDMDL KKYMDTIGEK DGLGPDMVKK FSYQLVKGLY YCHGHRILHR DLKPQNLLIN KSGDLKIGDF GLARAFGIPL RTYTHEVVTL WYRAPEVLLG SRHYSTAIDM WSVGCIVAEM ATRQPLFPGD SEIDEIFRIF RVLGTPDEDV WPGVRGLPDY KPTFPQWHPV ELGDVIKGFE ADGIDLIAQT LVYDPAHRIS AKRALQHPYF DTVNLAAA

NT Sequence

atgtcgcttg acaactatca aaagatcgaa aaggtcggag aaggcaccta tggtgtggtg tacaaggcca aagacatcaa cacgggcaac attgtcgccc tcaagaagat tcgtcttgaa gcagaagatg aaggtgtgcc aagcacttcg atcagagaaa tcagtttgtt gaaagagttg agcaaggatg ataacattgt caagttgctg gatatcgtgc attctgaagc aaagctttac ctcgtgttcg agtttcttga tatggatttg aaaaagtata tggataccat tggtgaaaag gacggtctcg gtcccgacat ggtcaagaaa ttctcttacc aactcgtcaa aggtctctac tactgccacg gccaccgtat tcttcatcga gaccttaaac ctcaaaatct gttgatcaac aagtctggtg atctcaagat tggtgatttc ggtttggcga gggcgtttgg tatccctctg cgtacttata ctcacgaagt tgtgacattg tggtaccgag ctcccgaagt cctccttggc tcaaggcatt actctaccgc tattgatatg tggtccgttg gatgtatcgt cgctgaaatg gccactcgtc aacctctctt cccgggtgac tctgagattg atgaaatctt tcgaattttc cgagttctcg gtacgcccga cgaagacgta tggcccggag tcaggggctt gcccgattac aagcctacct ttccccaatg gcaccccgtc gaactggggg atgtcatcaa gggctttgag gccgatggga ttgacttgat tgcccagact ttagtctatg atcctgctca ccgaatttca gccaagcgcg cacttcaaca cccctacttt gacactgtca acctcgccgc cgcataa

Details for CrneC.01010.e.B1.GE41262

HARVESTED ON:	3/8/2017
SEQUENCED ON:	3/10/2017
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS LDNYQKIEKV GEGTYGVVYK AKDINTGNIV ALKKIRLEAE DEGVPSTSIR EISLLKELSK DDNIVKLLDI VHSEAKLYLV FEFLDMDLKK YMDTIGEKDG LGPDMVKKFS YQLVKGLYYC HGHRILHRDL KPQNLLINKS GDLKIGDFGL ARAFGIPLRT YTHEVVTLWY RAPEVLLGSR HYSTAIDMWS VGCIVAEMAT RQPLFPGDSE IDEIFRIFRV LGTPDEDVWP GVRGLPDYKP TFPQWHPVEL GDVIKGFEAD GIDLIAQTLV YDPAHRISAK RALQHPYFXX XNLAAA

Validated NT Sequence

atcttactca cttatgcggc ggcgaggttg annnnnncaa agtaggggtg ttgaagtgcg cgcttggctg aaattcggtg agcaggatca tagactaaag tctgggcaat caagtcaatc ccatcggcct caaagccctt gatgacatcc cccagttcga cggggtgcca ttggggaaag gtaggcttgt aatcgggcaa gcccctgact ccgggccata cgtcttcgtc gggcgtaccg agaactcgga aaattcgaaa gatttcatca atctcagagt cacccgggaa gagaggttga cgagtggcca tttcagcgac gatacatcca acggaccaca tatcaatagc ggtagagtaa tgccttgagc caaggaggac ttcgggagct cggtaccaca atgtcacaac ttcgtgagta taagtacgca gagggatacc aaacgccctc gccaaaccga aatcaccaat cttgagatca ccagacttgt tgatcaacag attttgaggt ttaaggtctc gatgaagaat acggtggccg tggcagtagt agagaccttt gacgagttgg taagagaatt tcttgaccat gtcgggaccg agaccgtcct tttcaccaat ggtatccata tactttttca aatccatatc aagaaactcg aacacgaggt aaagctttgc ttcagaatgc acgatatcca gcaacttgac aatgttatca tccttgctca actctttcaa caaactgatt tctctgatcg aagtgcttgg cacaccttca tcttctgctt caagacgaat cttcttgagg gcgacaatgt tgcccgtgtt gatgtctttg gccttgtaca ccacaccata ggtgccttct ccgacctttt cgatcttttg atagttgtca agcgacatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgc ttgacaacta tcaaaagatc gaaaaggtcg gagaaggcac ctatggtgtg gtgtacaagg ccaaagacat caacacgggc aacattgtcg ccctcaagaa gattcgtctt gaagcagaag atgaaggtgt gccaagcact tcgatcagag aaatcagttt gttgaaagag ttgagcaagg atgataacat tgtcaagttg ctggatatcg tgcattctga agcaaagctt tacctcgtgt tcgagtttct tgatatggat ttgaaaaagt atatggatac cattggtgaa aaggacggtc tcggtcccga catggtcaag aaattctctt accaactcgt caaaggtctc tactactgcc acggccaccg tattcttcat cgagacctta aacctcaaaa tctgttgatc aacaagtctg gtgatctcaa gattggtgat ttcggtttgg cgagggcgtt tggtatccct ctgcgtactt atactcacga agttgtgaca ttgtggtacc gagctcccga agtcctcctt ggctcaaggc attactctac cgctattgat atgtggtccg ttggatgtat cgtcgctgaa atggccactc gtcaacctct cttcccgggt gactctgaga ttgatgaaat ctttcgaatt ttccgagttc tcggtacgcc cgacgaagac gtatggcccg gagtcagggg cttgcccgat tacaagccta cctttcccca atggcacccc gtcgaactgg gggatgtcat caagggcttt gaggccgatg ggattgactt gattgcccag actttagtct atgatcctgc tcaccgaatt tcagccaagc gcgcacttca acacccctac tttgacactg tcaacctcgc cgccgcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at