CrneC.01010.d

Mitogen-activated protein kinase (EC 2.7.11.24)

CENTER ID: CrneC.01010.d
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
CrneC.01010.d.B1.GE41260 full length 1 398
CrneC.01010.d.B2.GE41261 Truncation 1 36 398
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_02531
UniProt: J9VLD9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTSTCRHAP DDKLVNDPNA AANAGQVQER TTKGSSSSAP RKVRFNVGNN YHVVDVIGEG AYGVVASAVH RPSGSKVAIK KIAPFDHSMF ALRTLRELKL LKYFAEEGVS ENIISVLDIV KPPSYDTFKE VYLVQELLET DLHRVIRTQD LSDDHCQYFL YQTCRALKAL HSAEIIHRDL KPSNLLLNAN CDLKVCDFGL ARSTQTAFPA EGNNQGFMTE YVATRWYRAP EVMLSFRMYT KSIDVWSVGC ILAEMLSGKP LFPGKDYHNQ LALILDVLGT PTIDEFHAIT SKRSKDYIRS LPFRKKRTFE SIYPNANPLA IDFLRKTLTF DPRKRYTVEQ CLAHPYLDAY HDPEDEPSAK PLPPSFFEFD MVKDDISREE LKRLLHEEIM GFRPVLEG
NT Sequence
atgtcaacgt ccacttgccg ccatgcccca gacgacaagc tcgtcaatga ccccaacgca gctgcaaacg ctggccaggt gcaggaacgt acgaccaagg gatcttcttc ctctgctcct cgcaaagtca ggttcaacgt cggaaacaac taccacgtag tcgatgtcat cggcgaaggc gcctatgggg tggtcgcgtc cgccgtccac cgtccatcag gtagcaaagt agctatcaag aagattgcac cgtttgatca ttcaatgttt gcgttgagga cattgagaga gctgaagctg ttgaagtact ttgcggagga gggtgttagt gagaatatta tctcagtgct ggatatcgtc aaaccacctt cttacgacac tttcaaagaa gtttatcttg tccaagaact tctcgaaaca gaccttcatc gcgtgatccg aacccaagac ttgagtgacg accactgtca atatttctta taccaaacat gtagagcttt gaaagctttg cattctgctg aaatcatcca ccgagacttg aaaccatcaa acctcttgtt aaacgccaat tgtgatctca aagtatgcga ttttggactc gcacggtcga cacaaaccgc tttcccggcg gagggcaata accagggatt catgactgaa tatgttgcaa caaggtggta tagagcacca gaagttatgc tttcgtttag aatgtacacc aagtcaatcg acgtatggtc agtgggatgt atactcgcag agatgttgag tggaaaaccc cttttcccgg ggaaagatta ccacaaccaa ctcgccctca tccttgatgt gttgggtaca cctactatcg atgaatttca tgccatcact tcaaagaggt caaaagatta tattcgatct ttaccattcc gaaagaagag gacatttgaa agtatttacc caaacgcgaa tccgctcgct atcgatttct tgagaaaaac ccttactttc gatccccgca aacgctacac agtcgaacaa tgtctcgccc acccttacct cgacgcctac cacgatccgg aagacgaacc gtccgcgaaa ccgctcccgc cctcgttctt tgagtttgat atggtgaaag atgatatttc gagggaagag ttgaagaggc tgttgcatga ggagattatg gggtttcgtc cggttttgga ggggtag
Details for CrneC.01010.d.B1.GE41260
HARVESTED ON: 3/8/2017
SEQUENCED ON: 3/9/2017
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS TSTCRHAPDD KLVNDPNAAA NAGQVQERTT KGSSSSAPRK VRFNVGNNYH VVDVIGEGAY GVVASAVHRP SGSKVAIKKI APFDHSMFAL RTLRELKLLK YFAEEGVSEN IISVLDIVKP PSYDTFKEVY LVQELLETDL HRVIRTQDLS DDHCQYFLYQ TCRALKALHS AEIIHRDLKP SNLLLNANCD LKVCDFGLAR STQTAFPAEG NNQGFMTEYV ATRWYRAPEV MLSFRMYTKS IDVWSVGCIL AEMLSGKPLF PGKDYHNQLA LILDVLGTPT IDEFHAITSK RSKDYIRSLP FRKKRTFESI YPNANPLAID FLRKTLTFDP RKRYTVEQCL AHPYLDAYHD PEDEPSAKPL PPSFFEFDMV KDDISREELK RLLHEEIMGF RPVLEG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa cgtccacttg ccgccatgcc ccagacgaca agctcgtcaa tgaccccaac gcagctgcaa acgctggcca ggtgcaggaa cgtacgacca agggatcttc ttcctctgct cctcgcaaag tcaggttcaa cgtcggaaac aactaccacg tagtcgatgt catcggcgaa ggcgcctatg gggtggtcgc gtccgccgtc caccgtccat caggtagcaa agtagctatc aagaagattg caccgtttga tcattcaatg tttgcgttga ggacattgag agagctgaag ctgttgaagt actttgcgga ggagggtgtt agtgagaata ttatctcagt gctggatatc gtcaaaccac cttcttacga cactttcaaa gaagtttatc ttgtccaaga acttctcgaa acagaccttc atcgcgtgat ccgaacccaa gacttgagtg acgaccactg tcaatatttc ttataccaaa catgtagagc tttgaaagct ttgcattctg ctgaaatcat ccaccgagac ttgaaaccat caaacctctt gttaaacgcc aattgtgatc tcaaagtatg cgattttgga ctcgcacggt cgacacaaac cgctttcccg gcggagggca ataaccaggg attcatgact gaatatgttg caacaaggtg gtatagagca ccagaagtta tgctttcgtt tagaatgtac accaagtcaa tcgacgtatg gtcagtggga tgtatactcg cagagatgtt gagtggaaaa ccccttttcc cggggaaaga ttaccacaac caactcgccc tcatccttga tgtgttgggt acacctacta tcgatgaatt tcatgccatc acttcaaaga ggtcaaaaga ttatattcga tctttaccat tccgaaagaa gaggacattt gaaagtattt acccaaacgc gaatccgctc gctatcgatt tcttgagaaa aacccttact ttcgatcccc gcaaacgcta cacagtcgaa caatgtctcg cccaccctta cctcgacgcc taccacgatc cggaagacga accgtccgcg aaaccgctcc cgccctcgtt ctttgagttt gatatggtga aagatgatat ttcgagggaa gagttgaaga ggctgttgca tgaggagatt atggggtttc gtccggtttt ggaggggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for CrneC.01010.d.B2.GE41261
HARVESTED ON: 3/8/2017
SEQUENCED ON: 3/9/2017
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS SSAPRKVRFN VGNNYHVVDV IGEGAYGVVA SAVHRPSGSK VAIKKIAPFD HSMFALRTLR ELKLLKYFAE EGVSENIISV LDIVKPPSYD TFKEVYLVQE LLETDLHRVI RTQDLSDDHC QYFLYQTCRA LKALHFAEII HRDLKPSNLL LNANCDLKVC DFGLARSTQT AFPAEGNNQG FMTEYVATRW YRAPEVMLSF RMYTKSIDVW SVGCILAEML SGKPLFPGKD YHNQLALILD VLGTPTIDEF HAITSKRSKD YIRSLPFRKK RTFESIYPNA NPLAIDFLRK TLTFDPRKRY TVEQCLAHPY LDAYHDPEDE PSAKPLPPSF FEFDMVKDDI SREELKRLLH EEI
Validated NT Sequence
atggctcacc accaccacca ccatatgtct tcctctgctc ctcgcaaagt caggttcaac gtcggaaaca actaccacgt agtcgatgtc atcggcgaag gcgcctatgg ggtggtcgcg tccgccgtcc accgtccatc aggtagcaaa gtagctatca agaagattgc accgtttgat cattcaatgt ttgcgttgag gacattgaga gagctgaagc tgttgaagta ctttgcggag gagggtgtta gtgagaatat tatctcagtg ctggatatcg tcaaaccacc ttcttacgac actttcaaag aagtttatct tgtccaagaa cttctcgaaa cagaccttca tcgcgtgatc cgaacccaag acttgagtga cgaccactgt caatatttct tataccaaac atgtagagct ttgaaagctt tgcattttgc tgaaatcatc caccgagact tgaaaccatc aaacctcttg ttaaacgcca attgtgatct caaagtatgc gattttggac tcgcacggtc gacacaaacc gctttcccgg cggagggcaa taaccaggga ttcatgactg aatatgttgc aacaaggtgg tatagagcac cagaagttat gctttcgttt agaatgtaca ccaagtcaat cgacgtatgg tcagtgggat gtatactcgc agagatgttg agtggaaaac cccttttccc ggggaaagat taccacaacc aactcgccct cattcttgat gtgttgggta cacctactat cgatgaattt catgccatca cttcaaagag gtcaaaagat tatattcgat ctttaccatt ccgaaagaag aggacatttg aaagtattta cccaaacgcg aatccgctcg ctatcgattt cttgagaaaa acccttactt tcgatccccg caaacgctac acagtcgaac aatgtctcgc ccacccttac ctcgacgcct accacgatcc ggaagacgaa ccgtccgcga aaccgctccc gccctcgttc tttgagtttg atatggtgaa agatgatatt tcgagggaag agttgaagag gctgttgcat gaggagatta tg
Expected Protein Sequence
MAHHHHHHSS SAPRKVRFNV GNNYHVVDVI GEGAYGVVAS AVHRPSGSKV AIKKIAPFDH SMFALRTLRE LKLLKYFAEE GVSENIISVL DIVKPPSYDT FKEVYLVQEL LETDLHRVIR TQDLSDDHCQ YFLYQTCRAL KALHSAEIIH RDLKPSNLLL NANCDLKVCD FGLARSTQTA FPAEGNNQGF MTEYVATRWY RAPEVMLSFR MYTKSIDVWS VGCILAEMLS GKPLFPGKDY HNQLALILDV LGTPTIDEFH AITSKRSKDY IRSLPFRKKR TFESIYPNAN PLAIDFLRKT LTFDPRKRYT VEQCLAHPYL DAYHDPEDEP SAKPLPPSFF EFDMVKDDIS REELKRLLHE EIMGFRPVLE G
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcttcct ctgctcctcg caaagtcagg ttcaacgtcg gaaacaacta ccacgtagtc gatgtcatcg gcgaaggcgc ctatggggtg gtcgcgtccg ccgtccaccg tccatcaggt agcaaagtag ctatcaagaa gattgcaccg tttgatcatt caatgtttgc gttgaggaca ttgagagagc tgaagctgtt gaagtacttt gcggaggagg gtgttagtga gaatattatc tcagtgctgg atatcgtcaa accaccttct tacgacactt tcaaagaagt ttatcttgtc caagaacttc tcgaaacaga ccttcatcgc gtgatccgaa cccaagactt gagtgacgac cactgtcaat atttcttata ccaaacatgt agagctttga aagctttgca ttctgctgaa atcatccacc gagacttgaa accatcaaac ctcttgttaa acgccaattg tgatctcaaa gtatgcgatt ttggactcgc acggtcgaca caaaccgctt tcccggcgga gggcaataac cagggattca tgactgaata tgttgcaaca aggtggtata gagcaccaga agttatgctt tcgtttagaa tgtacaccaa gtcaatcgac gtatggtcag tgggatgtat actcgcagag atgttgagtg gaaaacccct tttcccgggg aaagattacc acaaccaact cgccctcatc cttgatgtgt tgggtacacc tactatcgat gaatttcatg ccatcacttc aaagaggtca aaagattata ttcgatcttt accattccga aagaagagga catttgaaag tatttaccca aacgcgaatc cgctcgctat cgatttcttg agaaaaaccc ttactttcga tccccgcaaa cgctacacag tcgaacaatg tctcgcccac ccttacctcg acgcctacca cgatccggaa gacgaaccgt ccgcgaaacc gctcccgccc tcgttctttg agtttgatat ggtgaaagat gatatttcga gggaagagtt gaagaggctg ttgcatgagg agattatggg gtttcgtccg gttttggagg ggtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat