CrneC.01010.c

CMGC/CDK protein kinase

CENTER ID: CrneC.01010.c
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.01010.c.B2.GE41259 Truncation 1 427 748
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_05549
UniProt: J9VZV6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRFSSRDEEP EDGEVVEDDA VWKNPLATTK YLKSAPSASS SQKASSYKKS RPSFIPSIDA ADDDGHRPRR PPPPSLSSPY QQSRRRSSSH SRKPTSYEDE ERGSGMQYFL PKNPHSTETR EPEQRREDKY AKEPRSPHRR EGYYSSRKGD GRDYPPQLDR ERYGSVRRLE PGSDGGYGDR NRDRDLDRGG YRDRDDRRSY GRSSDSSTWG RLPREEYRRL EEFPRHPTGG RYREDSRERY PVDRDTDQRR SCSPVRIRSP ERFSDRPLTP VIDNGRTLSP DHGTKNMASS RTHRSSPSHS SNHGDHNGEK GGAPIKLNRN RHRPSLPLKP ISTSPPRSPS APPPPPPPDP PTLPPSPTEP PPPMPGKLSL EQTVPHAPNP RAQMATRPPS PKREEGEIRQ ETESVIFKFK LWTAEEELKA LGKNFGGSST LIRYNMGTKL GEGTFGVVTK AVENDTKRSV ALKKITQHNF RDGAHITTLR EIKILKSLQH PNVVPLLNMV ISRHDNHSEN TFIKNEVFMV FPYMDHDLCG LLSNNDFKVN HSGAKCIMKQ LLDGMAYIHS NNIIHRDIKT ANILVDKHGQ IMIADFGLAR PWTDNKKMPP HLATEYTNMV VTRWYRAPEL LLGCCNYGPA VDIWSIGCVL GEMYLRRPIL PGGGDREQLS MIFAKCGPLN EETWSGWQDL PGFPEAHGFA WDKTPRDTSI LEESKSWHMD RGGADLLVKL LSLDPSKRPT ASEALDHPWF WVSPKPAETI NLDFAASHEM SAWHKQPAAA PVQPPPQKAY QTQQAYQSQQ AHHAQQIYQP QQPYVAYQSG YGNQQRGHGQ GYQQQSANAT RHNPAMNPYA SSQVANAQGY NPQPYMQAQG LSNMPPGADA YGGVNPYGAS VAYNNVPLPP SNMAYNGQSS FNGRGGLTGG GGQQYSLSHQ PYLPPGPPSM MHTNVNRPSR PPPPTNSGGR PLAAAPFPLA GGGGGGMKTF APPPPFSLAG GGGGRGGMPP TMKRPPSDSR RDLGGDHKRQ RTEAPLPYD
NT Sequence
atgcgcttct catcccggga tgaagagcct gaagacggcg aggttgtaga agatgacgct gtctggaaaa accctttggc gacaactaaa tatttgaaga gcgctccttc tgcctcgtcc tctcagaaag catccagtta taagaaatcc aggccatcgt tcattccttc gatcgatgcg gcagatgacg atggacatcg cccccgacgt cctccacctc catcactttc ttctccttac cagcaatcac gtcgccgttc ctcgtctcac agcagaaaac ccacttcgta cgaagatgaa gagcggggct cggggatgca gtacttcttg ccaaaaaatc ctcattcaac agagacgcga gaaccagagc agcgaagaga agataaatat gcaaaggaac cacggtctcc tcatcgccgt gaaggatact actcttcgag aaagggggac ggacgggatt atcctcccca gcttgaccgt gagcgctatg gatcagtaag gcggcttgaa ccaggaagtg atggtggata cggagaccga aatcgagata gagatctgga tagaggcgga tatagagaca gggacgatcg tcgttcatat ggtcgatctt cagattcttc aacttgggga aggcttccac gtgaagagta taggaggctg gaagagtttc ctcgccatcc tactggagga aggtaccgag aagattcgcg cgaaagatac ccagtggata gggacactga tcaacgccga tcatgctcgc ctgttcgcat acgttcacct gaacgattta gcgaccggcc cttgacgcct gtgatagata atggtcgcac cttgtcacca gaccatggca ctaagaacat ggcatcctca cgaacccatc gttcaagtcc cagccattca tctaatcatg gagatcacaa cggcgaaaaa ggaggcgctc ccataaaact caaccgtaac agacatcgcc cttccttacc tttaaaacct atttctacat caccacctcg atcaccttct gcccctccac cacctccacc tcctgatccg ccaacacttc caccttctcc caccgaaccc ccacctccaa tgccaggaaa actttccctg gaacaaacag ttccccatgc gccgaaccct cgtgctcaga tggcaactcg gcctccatca ccaaaacggg aagaaggaga gataaggcag gaaacagaat ccgtaatttt caaattcaag ttatggactg cagaggagga gttgaaggca ttgggcaaga attttggagg atcgtcgact ttgataagat acaatatggg aactaagctt ggtgaaggga cctttggtgt tgtaacgaag gctgttgaga atgatacaaa aagatcagtt gcgctgaaaa aaatcacaca acataatttc cgtgatggag ctcatattac tacactacgt gaaatcaaga ttttgaagag cctgcaacat ccaaacgttg ttccgctttt aaatatggtc atatcacgac atgacaatca ttctgaaaac accttcatta aaaacgaggt tttcatggtc ttcccgtaca tggaccacga tttgtgcggt ttgcttagca acaacgactt caaggtgaac cattcaggag caaagtgtat catgaaacag cttctcgacg ggatggcata tatccactcc aacaacatta ttcatcgaga tatcaaaacg gccaatatcc ttgttgataa acacggtcaa atcatgattg ccgattttgg tctcgcgcgt ccatggacgg acaacaagaa gatgccacct catctggcga ccgaatatac taacatggta gtcacgcgat ggtaccgtgc gcccgaactt cttttgggat gctgcaacta cggtccagcg gtggacattt ggtcaatcgg ctgtgtgttg ggagaaatgt acctgcgcag accgatttta ccaggtggcg gggataggga acagttgagc atgatctttg ccaaatgcgg ccctttgaac gaagagacct ggtcggggtg gcaggattta cctggtttcc cagaggcgca tggtttcgcg tgggataaga cgccgcgaga tacgagtatt ttggaggaga gcaaatcctg gcatatggac cgtggtggag cagatctgtt ggtcaagcta ttatccctcg atccgagcaa gcggccaacg gcgagcgagg ctcttgatca tccttggttc tgggtctctc ccaagccggc agaaaccatc aaccttgatt ttgcggcttc acacgaaatg tcagcttggc ataaacaacc cgccgctgct ccagttcaac caccaccgca gaaggcttat caaactcaac aagcgtacca atctcagcaa gcgcaccacg ctcaacaaat ctaccagcct caacagccat atgttgctta tcagtctggc tatggtaacc agcagcgcgg ccatggtcaa ggttatcaac aacaaagcgc aaacgccacc aggcacaatc ctgcaatgaa tccttacgcg tcttcgcaag tggcgaatgc gcagggatac aatccgcaac cgtacatgca agcacaggga ttatctaata tgccacccgg tgcagacgct tatggtgggg taaacccata cggtgcctcc gttgcataca ataatgtccc tctccctcct tccaatatgg catataacgg gcaatcatca ttcaacggaa gaggcggttt aacaggcggc gggggtcaac agtattctct ttcacaccaa ccatatcttc caccaggccc cccatcaatg atgcacacca atgtcaatcg cccttctcga ccaccgcctc ccaccaatag tggagggcgg cctttagcag cagcgccttt cccgctggca ggaggaggag gaggaggaat gaaaactttt gcgccgccgc cacctttcag cctggcaggc ggaggtggcg gtagaggagg tatgccaccc acgatgaaga gacctccttc cgacagtcga agggatcttg gaggggatca taaaaggcaa agaacagagg cgccattacc ttatgattga
Details for CrneC.01010.c.B2.GE41259
HARVESTED ON: 3/8/2017
SEQUENCED ON: 3/9/2017
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG SSTLIRYNMG TKLGEGTFGV VTKAVENDTK RSVALKKITQ HNFRDGAHIT TLREIKILKS LQHPNVVPLL NMVISRHDNH SENTFIKNEV FMVFPYMDHD LCGLLSNNDF KVNHSGAKCI MKQLLDGMAY IHSNNIIHRD IKTVNILVDK HGQIMIADFG LARPWTDNKK MPPHLATEYT NMVVTRWYRA PELLLGCCNY GPAVDIWSIG CVLGEMYLRR PILPGGGDRE QLSMIFAKCG PLNEETWSGW QDLPGFPEAH GFAWDKTPRD TSILEESKSW HMDRGGADLL VKLLSLDPSK RPTASEALDH PWXXXXPKP
Validated NT Sequence
atggctcacc accaccacca ccatatggga tcgtcgactt tgataagata caatatggga actaagcttg gtgaagggac ctttggtgtt gtaacgaagg ctgttgagaa tgatacaaaa agatcagttg cgctgaaaaa aatcacacaa cataatttcc gtgatggagc tcatattact acactacgtg aaatcaagat tttgaagagc ctgcaacatc caaacgttgt tccgctttta aatatggtca tatcacgaca tgacaatcat tctgaaaaca ccttcattaa aaacgaggtt ttcatggtct tcccgtacat ggaccacgat ttgtgcggtt tgcttagcaa caacgacttc aaggtgaacc attcaggagc aaagtgtatc atgaaacagc ttctcgacgg gatggcatat atccactcca acaacattat tcatcgagat atcaaaacgg tcaatatcct tgttgataaa cacggtcaaa tcatgattgc cgattttggt ctcgcgcgtc catggacgga caacaagaag atgccacctc atctggcgac cgaatatact aacatggtag tcacgcgatg gtaccgtgcg cccgaacttc ttttgggatg ctgcaactac ggtccagcgg tggacatttg gtcaatcggc tgtgtgttgg gagaaatgta cctgcgcaga ccgattttac caggtggcgg ggatagggaa cagttgagca tgatctttgc caaatgcggc cctttgaacg aagagacctg gtcggggtgg caggatttac ctggtttccc agaggcgcat ggtttcgcgt gggataagac gccgcgagat acgagtattt tggaggagag caaatcctgg catatggacc gtggtggagc agatctgttg gtcaagctat tatccctcga tccgagcaag cggccaacgg cgagcgaggc tcttgatcat ccttggttnn nnnnnnnncc caagccgg
Expected Protein Sequence
MAHHHHHHGS STLIRYNMGT KLGEGTFGVV TKAVENDTKR SVALKKITQH NFRDGAHITT LREIKILKSL QHPNVVPLLN MVISRHDNHS ENTFIKNEVF MVFPYMDHDL CGLLSNNDFK VNHSGAKCIM KQLLDGMAYI HSNNIIHRDI KTANILVDKH GQIMIADFGL ARPWTDNKKM PPHLATEYTN MVVTRWYRAP ELLLGCCNYG PAVDIWSIGC VLGEMYLRRP ILPGGGDREQ LSMIFAKCGP LNEETWSGWQ DLPGFPEAHG FAWDKTPRDT SILEESKSWH MDRGGADLLV KLLSLDPSKR PTASEALDHP WFWVSPKPAE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggatcgt cgactttgat aagatacaat atgggaacta agcttggtga agggaccttt ggtgttgtaa cgaaggctgt tgagaatgat acaaaaagat cagttgcgct gaaaaaaatc acacaacata atttccgtga tggagctcat attactacac tacgtgaaat caagattttg aagagcctgc aacatccaaa cgttgttccg cttttaaata tggtcatatc acgacatgac aatcattctg aaaacacctt cattaaaaac gaggttttca tggtcttccc gtacatggac cacgatttgt gcggtttgct tagcaacaac gacttcaagg tgaaccattc aggagcaaag tgtatcatga aacagcttct cgacgggatg gcatatatcc actccaacaa cattattcat cgagatatca aaacggccaa tatccttgtt gataaacacg gtcaaatcat gattgccgat tttggtctcg cgcgtccatg gacggacaac aagaagatgc cacctcatct ggcgaccgaa tatactaaca tggtagtcac gcgatggtac cgtgcgcccg aacttctttt gggatgctgc aactacggtc cagcggtgga catttggtca atcggctgtg tgttgggaga aatgtacctg cgcagaccga ttttaccagg tggcggggat agggaacagt tgagcatgat ctttgccaaa tgcggccctt tgaacgaaga gacctggtcg gggtggcagg atttacctgg tttcccagag gcgcatggtt tcgcgtggga taagacgccg cgagatacga gtattttgga ggagagcaaa tcctggcata tggaccgtgg tggagcagat ctgttggtca agctattatc cctcgatccg agcaagcggc caacggcgag cgaggctctt gatcatcctt ggttctgggt ctctcccaag ccggcagaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat