SSGCID: Target Page

CrneC.01010.b

Mitogen-activated protein kinase (EC 2.7.11.24)

CENTER ID:	CrneC.01010.b
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.01010.b.B1.GE41258	full length		1	366

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_02511
UniProt:	J9VPG9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTIDQSQINF NVPSTYKLEE IVGEGAYGLV VAGTHLPSGT RVAIKRITPF DHTMFCQRTL REIKLLRHFR HENIISILDL IQPESYEVFN EVYLVQELME TDLHRVIRSQ ELSDDHCQYF VYQTLRGLKA LHSADVLHRD LKPSNLLLNA NCDLKICDFG LARSSAKPPP GASDGGQGFM TEYVATRWYR APEVMLSFQE YTKAIDLWSV GCILAEMING KPLFPGRDYH HQLSLILQVL GTPTMDDFNE ITSQRSKDYL RALEFTRRQD FSAICPKAKP AAVDLLKRTL TFSPSKRITV EEALTHPYVE AYHDPHDEPT AESLKPGFFD FEFHQEKLSR DQWKRMIYDE VQDPVPTILS QWTTSH

NT Sequence

atgacaatcg accaaagcca aatcaacttc aatgttcctt ctacttataa gctcgaagag atcgtgggtg aaggtgcata tggtctcgta gtagctggta cgcatcttcc ctctgggact cgagtcgcta tcaagcgtat cacgcctttt gaccatacca tgttctgcca acgaacgttg agagagatca agcttcttag gcattttcgt cacgaaaata tcatctccat tcttgaccta attcaacccg aaagttatga ggtgttcaac gaagtctatc tcgtccagga acttatggag actgatttgc accgagtgat cagaagtcag gaactatcag atgatcactg ccaatacttt gtctatcaaa ctcttcgggg tctgaaagcc ctccattccg ccgacgtcct tcaccgtgac ctcaagccct caaaccttct gttaaacgcc aactgcgacc tcaaaatctg cgatttcggt cttgcccgat catctgccaa gcctccacct ggtgcatcag acggaggaca gggattcatg acggaatatg tcgctacaag gtggtatcga gcgcctgaag tcatgttgtc attccaagaa tataccaagg ctatcgattt gtggagtgta ggatgcatcc ttgctgaaat gatcaatggc aagccgttat tccctgggag agattaccac caccaattat ctttgatctt acaagtgctc ggtaccccta ctatggacga cttcaacgag atcacctccc aacgatcaaa ggattacctt cgagcactag agtttacccg tcgtcaagat ttttcggcca tttgccctaa ggcaaagcct gcggcggttg atcttttaaa gcgaaccctc actttttccc catccaagag gataacagtg gaagaagcgt tgacgcaccc ttacgtcgag gcttatcacg acccacacga cgaacctact gctgagtccc tcaaacccgg attctttgac tttgagttcc accaggagaa attgagtaga gatcaatgga aacgaatgat ttacgatgag gtgcaagatc cagtacccac catcctctct caatggacga caagccatta a

Details for CrneC.01010.b.B1.GE41258

HARVESTED ON:	3/8/2017
SEQUENCED ON:	3/9/2017
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	81

Validated AA Sequence

MFCQRTLREI KLLRHFRHEN IISILDLIQP ESYEVFNEVY LVQELMETDL HRVIRSQELS DDHCQYFVYQ TLRGLKALHS ADVLHRDLKP SNLLLNANCD LKICDFGLAR SSAKPPPGAS DGGQGFMTEY VATRWYRAPE VMLSFQEYTK AIDLWSVGCI LAEMINGKPL FPGRDYHHQL SLILQVLGTP TMDDFNEITS QRSKDYLRAL EFTRRQDFSA ICPKAKPAAV DLLKRTLTFS PSKRITVEEA LTHPYVEAYH DPHDEPTAES LKPGFFDFEF HQEKLSRDQW KRMIYDEVQD PVPT

Validated NT Sequence

tcacgccttt tgaccatacc atgttctgcc aacgaacgtt gagagagatc aagcttctta ggcattttcg tcacgaaaat atcatctcca ttcttgacct aattcaaccc gaaagttatg aggtgttcaa cgaagtctat ctcgtccagg aacttatgga gactgatttg caccgagtga tcagaagtca ggaactatca gatgatcact gccaatactt tgtctatcaa actcttcggg gtctgaaagc cctccattcc gccgacgtcc ttcaccgtga cctcaagccc tcaaaccttc tgttaaacgc caactgcgac ctcaaaatct gcgatttcgg tcttgcccga tcatctgcca agcctccacc tggtgcatca gacggaggac agggattcat gacggaatat gtcgctacaa ggtggtatcg agcgcctgaa gtcatgttgt cattccaaga atataccaag gctatcgatt tgtggagtgt aggatgcatc cttgctgaaa tgatcaatgg caagccgtta ttccctggga gagattacca ccaccaatta tctttgatct tacaagtgct cggtacccct actatggacg acttcaacga gatcacctcc caacgatcaa aggattacct tcgagcacta gagtttaccc gtcgtcaaga tttttcggcc atttgcccta aggcaaagcc tgcggcggtt gatcttttaa agcgaaccct cactttttcc ccatccaaga ggataacagt ggaagaagcg ttgacgcacc cttacgtcga ggcttatcac gacccacacg acgaacctac tgctgagtcc ctcaaacccg gattctttga ctttgagttc caccaggaga aattgagtag agatcaatgg aaacgaatga tttacgatga ggtgcaagat ccagtaccca ccat

Expected Protein Sequence

MAHHHHHHMT IDQSQINFNV PSTYKLEEIV GEGAYGLVVA GTHLPSGTRV AIKRITPFDH TMFCQRTLRE IKLLRHFRHE NIISILDLIQ PESYEVFNEV YLVQELMETD LHRVIRSQEL SDDHCQYFVY QTLRGLKALH SADVLHRDLK PSNLLLNANC DLKICDFGLA RSSAKPPPGA SDGGQGFMTE YVATRWYRAP EVMLSFQEYT KAIDLWSVGC ILAEMINGKP LFPGRDYHHQ LSLILQVLGT PTMDDFNEIT SQRSKDYLRA LEFTRRQDFS AICPKAKPAA VDLLKRTLTF SPSKRITVEE ALTHPYVEAY HDPHDEPTAE SLKPGFFDFE FHQEKLSRDQ WKRMIYDEVQ DPVPTILSQW TTSH

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa tcgaccaaag ccaaatcaac ttcaatgttc cttctactta taagctcgaa gagatcgtgg gtgaaggtgc atatggtctc gtagtagctg gtacgcatct tccctctggg actcgagtcg ctatcaagcg tatcacgcct tttgaccata ccatgttctg ccaacgaacg ttgagagaga tcaagcttct taggcatttt cgtcacgaaa atatcatctc cattcttgac ctaattcaac ccgaaagtta tgaggtgttc aacgaagtct atctcgtcca ggaacttatg gagactgatt tgcaccgagt gatcagaagt caggaactat cagatgatca ctgccaatac tttgtctatc aaactcttcg gggtctgaaa gccctccatt ccgccgacgt ccttcaccgt gacctcaagc cctcaaacct tctgttaaac gccaactgcg acctcaaaat ctgcgatttc ggtcttgccc gatcatctgc caagcctcca cctggtgcat cagacggagg acagggattc atgacggaat atgtcgctac aaggtggtat cgagcgcctg aagtcatgtt gtcattccaa gaatatacca aggctatcga tttgtggagt gtaggatgca tccttgctga aatgatcaat ggcaagccgt tattccctgg gagagattac caccaccaat tatctttgat cttacaagtg ctcggtaccc ctactatgga cgacttcaac gagatcacct cccaacgatc aaaggattac cttcgagcac tagagtttac ccgtcgtcaa gatttttcgg ccatttgccc taaggcaaag cctgcggcgg ttgatctttt aaagcgaacc ctcacttttt ccccatccaa gaggataaca gtggaagaag cgttgacgca cccttacgtc gaggcttatc acgacccaca cgacgaacct actgctgagt ccctcaaacc cggattcttt gactttgagt tccaccagga gaaattgagt agagatcaat ggaaacgaat gatttacgat gaggtgcaag atccagtacc caccatcctc tctcaatgga cgacaagcca ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat