SSGCID: Target Page

CrneC.01010.a

CAMK/CAMK1 protein kinase

CENTER ID:	CrneC.01010.a
ORGANISM:	Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE:	Pneumonia-like illness
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.01010.a.B1.GE41256	full length		1	362

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
CrneC.01010.a.B1.PS38288	full length		1	362

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	FungiDB:CNAG_04272
UniProt:	J9VRC6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAPQTVPCQY KTGKTLGSGT YAVVKECVHI TTGEYYACKV LNKKFLMGRE HMVRNEIAVL KRVSAGHKNI VQLHDFFETT HNLYLVFDLC TGGELFDRIC AKGSYFEKDA ANIVRTVTSA VKYLHDQGIV HRDLKPENIL FKSKAEDADL MLADFGLSKV LDDDKFAILT TTCGTPGYMA PEIFKKAGHG KPVDIWAIGV ITYFLLCGYT PFDRDSQYEE MQAICKGDYR FEPAEYWTGV SETAKQFVRD CLTIDPTNRP TANELLNHPW LRDVEATKDE PAEAKGVDLL PNVKAAFDAK KTFRKAVLSM MAVHRLQDHG ATIHGQTEAE QEKLKKEVEA FKEEAAQEDV KDVIQPGAPA SI

NT Sequence

atggcccctc agactgtccc ctgccagtac aagactggaa agacattagg aagtggaacg tacgccgtgg tcaaagaatg tgttcacatc acgactggtg aatactacgc atgtaaggta cttaataaga agttcttgat gggccgagag catatggtcc ggaacgaaat cgctgtcctt aaacgagttt cagctggcca caagaacatt gttcaattgc atgacttttt cgagactact cataaccttt atcttgtctt tgacctttgt accggtggag agcttttcga taggatctgt gccaagggaa gctactttga gaaggatgct gccaatattg tgcgaactgt aacatcagcc gtcaagtacc tccatgacca aggcatcgtt cacagagatt tgaagccgga gaacatcctt ttcaagtcca aggccgaaga tgcggatctt atgttggctg actttggatt gtccaaggtc ttagatgatg ataaatttgc tattctaact actacatgtg gtactcctgg ttacatggct cctgaaatat tcaaaaaggc cggccacggc aagcctgtgg atatttgggc catcggtgtc atcacctact tcctcctttg tggctatacc cccttcgacc gcgactctca atacgaagag atgcaagcca tctgcaaggg cgactatcgc ttcgaacctg ccgaatattg gacgggtgtc tctgagactg ccaaacaatt cgtgcgtgac tgcttgacca tcgaccccac aaatcgacct acagcgaatg agttgttgaa ccacccttgg ttgagagacg ttgaagccac caaggacgaa cccgctgagg ctaagggtgt tgacttgttg cctaacgtca aggccgcctt cgatgccaag aagacattcc gcaaggcagt tttgagtatg atggctgttc acaggctcca agatcatggc gccactatcc acggtcagac tgaggcagag caggaaaaat tgaagaagga agtggaagcg ttcaaggaag aggcggctca ggaggacgtg aaggatgtta tccaaccagg tgctcccgct tccatttaa

Details for CrneC.01010.a.B1.GE41256

HARVESTED ON:	3/8/2017
SEQUENCED ON:	3/9/2017
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMA PQTVPCQYKT GKTLGSGTYA VVKECVHITT GEYYACKVLN KKFLMGREHM VRNEIAVLKR VSAGHKNIVQ LHDFFETTHN LYLVFDLCTG GELFDRICAK GSYFEKDAAN IVRTVTSAVK YLHDQGIVHR DLKPENILFK SKAEDADLML ADFGLSKVLD DDKFAILTTT CGTPGYMAPE IFKKAGHGKP VDIWAIGVIT YFLLCGYTPF DRDSQYEEMQ AICKGDYRFE PAEYWTGVSE TAKQFVRDCL TIDPTNRPTA NELLNHPWLR DVEATKDEPA EAKGVDLLPN VKAAFDAKKT FRKAVLSMMA VHRLQDHGAT IHGQTEAEQE KLKKEVEAFK EEAAQEDVKD VI

Validated NT Sequence

atggctcacc accaccacca ccatatggcc cctcagactg tcccctgcca gtacaagact ggaaagacat taggaagtgg aacgtacgcc gtggtcaaag aatgtgttca catcacgact ggtgaatact acgcatgtaa ggtacttaat aagaagttct tgatgggccg agagcatatg gtccggaacg aaatcgctgt ccttaaacga gtttcagctg gccacaagaa cattgttcaa ttgcatgact ttttcgagac tactcataac ctttatcttg tctttgacct ttgtaccggt ggagagcttt tcgataggat ctgtgccaag ggaagctact ttgagaagga tgctgccaat attgtgcgaa ctgtaacatc agccgtcaag tacctccatg accaaggcat cgttcacaga gatttgaagc cggagaacat ccttttcaag tccaaggccg aagatgcgga tcttatgttg gctgactttg gattgtccaa ggtcttagat gatgataaat ttgctattct aactactaca tgtggtactc ctggttacat ggctcctgaa atattcaaaa aggccggcca cggcaagcct gtggatattt gggccatcgg tgtcatcacc tacttcctcc tttgtggcta tacccccttc gaccgcgact ctcaatacga agagatgcaa gccatctgca agggcgacta tcgcttcgaa cctgccgaat attggacggg tgtctctgag actgccaaac aattcgtgcg tgactgcttg accatcgacc ccacaaatcg acctacagcg aatgagttgt tgaaccaccc ttggttgaga gacgttgaag ccaccaagga cgaacccgct gaggctaagg gtgttgactt gttgcctaac gtcaaggccg ccttcgatgc caagaagaca ttccgcaagg cagttttgag tatgatggct gttcacaggc tccaagatca tggcgccact atccacggtc agactgaggc agagcaggaa aaattgaaga aggaagtgga agcgttcaag gaagaggcgg ctcaggagga cgtgaaggat gttatcca

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccc ctcagactgt cccctgccag tacaagactg gaaagacatt aggaagtgga acgtacgccg tggtcaaaga atgtgttcac atcacgactg gtgaatacta cgcatgtaag gtacttaata agaagttctt gatgggccga gagcatatgg tccggaacga aatcgctgtc cttaaacgag tttcagctgg ccacaagaac attgttcaat tgcatgactt tttcgagact actcataacc tttatcttgt ctttgacctt tgtaccggtg gagagctttt cgataggatc tgtgccaagg gaagctactt tgagaaggat gctgccaata ttgtgcgaac tgtaacatca gccgtcaagt acctccatga ccaaggcatc gttcacagag atttgaagcc ggagaacatc cttttcaagt ccaaggccga agatgcggat cttatgttgg ctgactttgg attgtccaag gtcttagatg atgataaatt tgctattcta actactacat gtggtactcc tggttacatg gctcctgaaa tattcaaaaa ggccggccac ggcaagcctg tggatatttg ggccatcggt gtcatcacct acttcctcct ttgtggctat acccccttcg accgcgactc tcaatacgaa gagatgcaag ccatctgcaa gggcgactat cgcttcgaac ctgccgaata ttggacgggt gtctctgaga ctgccaaaca attcgtgcgt gactgcttga ccatcgaccc cacaaatcga cctacagcga atgagttgtt gaaccaccct tggttgagag acgttgaagc caccaaggac gaacccgctg aggctaaggg tgttgacttg ttgcctaacg tcaaggccgc cttcgatgcc aagaagacat tccgcaaggc agttttgagt atgatggctg ttcacaggct ccaagatcat ggcgccacta tccacggtca gactgaggca gagcaggaaa aattgaagaa ggaagtggaa gcgttcaagg aagaggcggc tcaggaggac gtgaaggatg ttatccaacc aggtgctccc gcttccattt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for CrneC.01010.a.B1.PS38288

PURIFICATION DATe:	5/23/2017
CONCENTRATION:	45mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer: 20mM HEPES, 300mM NaCl, 5% Glycerol, 1m MTCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	15
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCC CTCAGACTGT CCCCTGCCAG TACAAGACTG GAAAGACATT AGGAAGTGGA ACGTACGCCG TGGTCAAAGA ATGTGTTCAC ATCACGACTG GTGAATACTA CGCATGTAAG GTACTTAATA AGAAGTTCTT GATGGGCCGA GAGCATATGG TCCGGAACGA AATCGCTGTC CTTAAACGAG TTTCAGCTGG CCACAAGAAC ATTGTTCAAT TGCATGACTT TTTCGAGACT ACTCATAACC TTTATCTTGT CTTTGACCTT TGTACCGGTG GAGAGCTTTT CGATAGGATC TGTGCCAAGG GAAGCTACTT TGAGAAGGAT GCTGCCAATA TTGTGCGAAC TGTAACATCA GCCGTCAAGT ACCTCCATGA CCAAGGCATC GTTCACAGAG ATTTGAAGCC GGAGAACATC CTTTTCAAGT CCAAGGCCGA AGATGCGGAT CTTATGTTGG CTGACTTTGG ATTGTCCAAG GTCTTAGATG ATGATAAATT TGCTATTCTA ACTACTACAT GTGGTACTCC TGGTTACATG GCTCCTGAAA TATTCAAAAA GGCCGGCCAC GGCAAGCCTG TGGATATTTG GGCCATCGGT GTCATCACCT ACTTCCTCCT TTGTGGCTAT ACCCCCTTCG ACCGCGACTC TCAATACGAA GAGATGCAAG CCATCTGCAA GGGCGACTAT CGCTTCGAAC CTGCCGAATA TTGGACGGGT GTCTCTGAGA CTGCCAAACA ATTCGTGCGT GACTGCTTGA CCATCGACCC CACAAATCGA CCTACAGCGA ATGAGTTGTT GAACCACCCT TGGTTGAGAG ACGTTGAAGC CACCAAGGAC GAACCCGCTG AGGCTAAGGG TGTTGACTTG TTGCCTAACG TCAAGGCCGC CTTCGATGCC AAGAAGACAT TCCGCAAGGC AGTTTTGAGT ATGATGGCTG TTCACAGGCT CCAAGATCAT GGCGCCACTA TCCACGGTCA GACTGAGGCA GAGCAGGAAA AATTGAAGAA GGAAGTGGAA GCGTTCAAGG AAGAGGCGGC TCAGGAGGAC GTGAAGGATG TTATCCAACC AGGTGCTCCC GCTTCCATTT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT