CrneC.00844.a

Serine/threonine-protein kinase RIO1 (EC 2.7.11.1)

CENTER ID: CrneC.00844.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.00844.a.B2.GE41254 Truncation 1 136 407
CrneC.00844.a.B3.GE41255 Truncation 136 465
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_06454
UniProt: J9VWQ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRRPDLGYI DQASEDPVNT VSTPVEDLGQ TPHEGVEAEA PEEDSGSSIE EESEGEPFEA VDGDDFDFSA LNAGGSGEEE EEEDDGDWDV DDEDWELANG DFTKQYNRVR QQHAATSGSA PLPARNLSQQ TKSKLSKSNP TAGSGVVTNP KAAQDKHDKD KSDRATQEQV LDGRTRLVLA GLVNRGVIGM IERCISTGKE ANVYYSSPNR AVKIYRTSIL VFRARQNYIV GEQRFRGEYT SSRNPRKMIR VWAEKELRNL RRLVQGGVRA PVVHECKENV LVMDFLGKGE VASPRLKDAE IPEDRLPDLY AEMIIATRRM YQHCHLVHAD LSEYNILLHD NHLYIIDVSQ SVEHDHPRAF DFLRSDISNI EEFFSRRGVA TLGIRKSWEF IVTENIGLSP TGSASSSLEM EKGDQGERRL VDVLEKWLKE PSDKTDDAVF MESYIPRTLA EVYDPERDVD VLKSGGGDEL IYAGVTGLKL AHESANAKEK EKEKEKEKQS ASAGANKGEG VDVDESVEKG KKGKGKKSKD EEGKNEKSPK SVRFEDEVDE EQDEDDDAQK AEEEDEEEGM DKKSRGFRHE DRESKKERKK AVKEEQREKR KTKMPKAEKQ KLIKKSASRH
NT Sequence
atgtctagac gaccagacct cggctatatc gaccaagctt cagaagaccc ggtcaatacc gtctcaacac cagtcgaaga ccttgggcaa accccccacg aaggggtgga ggctgaagcg cctgaagaag actctggatc gagtattgaa gaagagtctg agggggagcc gtttgaggcc gtggatggag acgatttcga tttcagcgcg ttaaatgctg gtgggtcggg tgaagaggag gaggaggagg atgatggaga ttgggatgtg gacgatgagg attgggagct tgccaatgga gattttacca agcaatataa ccgagttcga cagcaacacg ctgccacatc cggttctgcc cccttacctg cgcgaaacct ctcccaacaa acaaaatcaa aattgtccaa atccaaccct acagccggat ctggagtggt gacaaatccg aaagcagcac aggataaaca tgataaagat aaaagcgatc gggctactca ggagcaagtg cttgatgggc gtacacgact ggttttggcg ggattagtca atcggggggt tatcggtatg attgaacggt gtatcagtac aggtaaagag gcaaacgtct actattcatc ccctaaccgt gctgtcaaaa tctaccgaac ctccatcctc gtcttccgtg ctcgacaaaa ctacattgta ggcgaacaac gtttccgggg cgaatatact tcttctcgaa atcctcgaaa gatgatccgt gtctgggctg aaaaggagtt gcgaaacctg agacggttgg ttcaaggagg tgtgcgtgca ccagtggtac atgaatgtaa ggagaatgtg ctcgtcatgg acttcttggg caagggcgag gtagcgtcgc ctcgtttgaa agacgcagag attcccgaag acaggttacc cgatctgtac gcagaaatga ttattgcgac tcgaagaatg tatcagcact gccacctcgt ccacgccgat ttgagtgaat acaacattct gttacacgac aaccacttat acatcatcga cgtctcgcaa tctgtcgaac acgaccaccc gcgcgcattc gatttcctcc gttccgacat ctccaacatt gaagaattct tctcccgccg tggggtcgct actctcggaa tccggaaatc atgggaattc atcgtcaccg agaacattgg tctttccccc actggctccg cctcgtcatc attggagatg gagaagggtg atcaaggcga aaggcgtttg gtggatgtct tggaaaaatg gttaaaagaa ccgtcagaca agacggacga tgcggtcttt atggaatcgt atatcccgag aacgttggcg gaggtgtatg atcccgagag ggatgtggat gtgctcaaga gtgggggagg ggatgagttg atttatgcgg gtgtgacggg tttaaagttg gcgcatgaga gtgcgaatgc caaggagaag gagaaggaga aagagaaaga gaaacagagt gctagtgcag gtgcgaacaa gggcgagggg gtggatgtgg atgagagtgt agaaaagggt aagaagggta aaggtaaaaa aagtaaggat gaggagggca aaaacgaaaa atcaccgaaa agcgtacggt ttgaggatga agtagacgaa gaacaagacg aggacgatga tgcccaaaaa gcagaggagg aagatgagga agaagggatg gataaaaaat ctagagggtt ccggcacgaa gatcgtgaat ctaaaaagga acgaaaaaag gcagtcaagg aagaacagag ggagaaacga aagaccaaga tgccaaaagc tgagaagcaa aaattgataa aaaagtctgc ctctcgacat tag
Details for CrneC.00844.a.B2.GE41254
HARVESTED ON: 3/8/2017
SEQUENCED ON: 3/9/2017
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS KSNPTAGSGV VTNPKAAQDK HDKDKSDRAT QEQVLDGRTR LVLAGLVNRG VIGMIERCIS TGKEANVYYS SPNRAVKIYR TSILVFRARQ NYIVGEQRFR GEYTSSRNPR KMIRVWAEKE LRNLRRLVQG GVRAPVVHEC KENVLVMDFL GKGEVASPRL KDAEIPEDRL PDLYAEMIIA TRRMYQHCHL VHADLSEYNI LLHDNHLYII DVSQSVEHDH PRAFDFLRSD ISNIEEFFSR RGVATLGIRK SWEFIVTENI GLSXLAPPRH HK
Validated NT Sequence
atggctcacc accaccacca ccatatgtcc aaatccaacc ctacagccgg atctggagtg gtgacaaatc cgaaagcagc acaggataaa catgataaag ataaaagcga tcgggctact caggagcaag tgcttgatgg gcgtacacga ctggttttgg cgggattagt caatcggggg gttatcggta tgattgaacg gtgtatcagt acaggtaaag aggcaaacgt ctactattca tcccctaacc gtgctgtcaa aatctaccga acctccatcc tcgtcttccg tgctcgacaa aactacattg taggcgaaca acgtttccgg ggcgaatata cttcttctcg aaatcctcga aagatgatcc gtgtctgggc tgaaaaggag ttgcgaaacc tgagacggtt ggttcaagga ggtgtgcgtg caccagtggt acatgaatgt aaggagaatg tgctcgtcat ggacttcttg ggcaagggcg aggtagcgtc gcctcgtttg aaagacgcag agattcccga agacaggtta cccgatctgt acgcagaaat gattattgcg actcgaagaa tgtatcagca ctgccacctc gtccacgccg atttgagtga atacaacatt ctgttacacg acaaccactt atacatcatc gacgtctcgc aatctgtcga acacgaccac ccgcgcgcat tcgatttcct ccgttccgac atctccaaca ttgaagaatt cttctcccgc cgtggggtcg ctactctcgg aatccggaaa tcatgggaat tcatcgtcac cgagaacatt ggtctttccc nactggctcc gcctcgtcat cataagtgag taagat
Expected Protein Sequence
MAHHHHHHSK SNPTAGSGVV TNPKAAQDKH DKDKSDRATQ EQVLDGRTRL VLAGLVNRGV IGMIERCIST GKEANVYYSS PNRAVKIYRT SILVFRARQN YIVGEQRFRG EYTSSRNPRK MIRVWAEKEL RNLRRLVQGG VRAPVVHECK ENVLVMDFLG KGEVASPRLK DAEIPEDRLP DLYAEMIIAT RRMYQHCHLV HADLSEYNIL LHDNHLYIID VSQSVEHDHP RAFDFLRSDI SNIEEFFSRR GVATLGIRKS WEFIVTENIG LSPTGSASSS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccaaat ccaaccctac agccggatct ggagtggtga caaatccgaa agcagcacag gataaacatg ataaagataa aagcgatcgg gctactcagg agcaagtgct tgatgggcgt acacgactgg ttttggcggg attagtcaat cggggggtta tcggtatgat tgaacggtgt atcagtacag gtaaagaggc aaacgtctac tattcatccc ctaaccgtgc tgtcaaaatc taccgaacct ccatcctcgt cttccgtgct cgacaaaact acattgtagg cgaacaacgt ttccggggcg aatatacttc ttctcgaaat cctcgaaaga tgatccgtgt ctgggctgaa aaggagttgc gaaacctgag acggttggtt caaggaggtg tgcgtgcacc agtggtacat gaatgtaagg agaatgtgct cgtcatggac ttcttgggca agggcgaggt agcgtcgcct cgtttgaaag acgcagagat tcccgaagac aggttacccg atctgtacgc agaaatgatt attgcgactc gaagaatgta tcagcactgc cacctcgtcc acgccgattt gagtgaatac aacattctgt tacacgacaa ccacttatac atcatcgacg tctcgcaatc tgtcgaacac gaccacccgc gcgcattcga tttcctccgt tccgacatct ccaacattga agaattcttc tcccgccgtg gggtcgctac tctcggaatc cggaaatcat gggaattcat cgtcaccgag aacattggtc tttcccccac tggctccgcc tcgtcatcat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for CrneC.00844.a.B3.GE41255
HARVESTED ON: 3/8/2017
SEQUENCED ON: 3/9/2017
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS KSNPTAGSGV VTNPKAAQDK HDKDKSDRAT QEQVLDGRTR LVLAGLVNRG VIGMIERCIS TGKEANVYYS SPNRAVKIYR TSILVFRARQ NYIVGEQRFR GEYTSSRNPR KMIRVWAEKE LRNLRRLVQG GVRAPVVHEC KENVLVMDFL GKGEVASPRL KDAEIPEDRL PDLYAEMIIA TRRMYQHCHL VHADLSEYNI LLHDNHLYII DVSQSVEHDH PRAFDFLRSD ISNIEEFFSR RGVATLGIRK SWEFIVTENI GLSPTGSASS SLEMEKGDQG ERRLVDVLEK WLKEPSDKTD DAVFMESYIP RTLAEVYDPE
Validated NT Sequence
atggctcacc accaccacca ccatatgtcc aaatccaacc ctacagccgg atctggagtg gtgacaaatc cgaaagcagc acaggataaa catgataaag ataaaagcga tcgggctact caggagcaag tgcttgatgg gcgtacacga ctggttttgg cgggattagt caatcggggg gttatcggta tgattgaacg gtgtatcagt acaggtaaag aggcaaacgt ctactattca tcccctaacc gtgctgtcaa aatctaccga acctccatcc tcgtcttccg tgctcgacaa aactacattg taggcgaaca acgtttccgg ggcgaatata cttcttctcg aaatcctcga aagatgatcc gtgtctgggc tgaaaaggag ttgcgaaacc tgagacggtt ggttcaagga ggtgtgcgtg caccagtggt acatgaatgt aaggagaatg tgctcgtcat ggacttcttg ggcaagggcg aggtagcgtc gcctcgtttg aaagacgcag agattcccga agacaggtta cccgatctgt acgcagaaat gattattgcg actcgaagaa tgtatcagca ctgccacctc gtccacgccg atttgagtga atacaacatt ctgttacacg acaaccactt atacatcatc gacgtctcgc aatctgtcga acacgaccac ccgcgcgcat tcgatttcct ccgttccgac atctccaaca ttgaagaatt cttctcccgc cgtggggtcg ctactctcgg aatccggaaa tcatgggaat tcatcgtcac cgagaacatt ggtctttccc ccactggctc cgcctcgtca tcattggaga tggagaaggg tgatcaaggc gaaaggcgtt tggtggatgt cttggaaaaa tggttaaaag aaccgtcaga caagacggac gatgcggtct ttatggaatc gtatatcccg agaacgttgg cggaggtgta tgatcccgag ag
Expected Protein Sequence
MAHHHHHHSK SNPTAGSGVV TNPKAAQDKH DKDKSDRATQ EQVLDGRTRL VLAGLVNRGV IGMIERCIST GKEANVYYSS PNRAVKIYRT SILVFRARQN YIVGEQRFRG EYTSSRNPRK MIRVWAEKEL RNLRRLVQGG VRAPVVHECK ENVLVMDFLG KGEVASPRLK DAEIPEDRLP DLYAEMIIAT RRMYQHCHLV HADLSEYNIL LHDNHLYIID VSQSVEHDHP RAFDFLRSDI SNIEEFFSRR GVATLGIRKS WEFIVTENIG LSPTGSASSS LEMEKGDQGE RRLVDVLEKW LKEPSDKTDD AVFMESYIPR TLAEVYDPER DVDVLKSG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccaaat ccaaccctac agccggatct ggagtggtga caaatccgaa agcagcacag gataaacatg ataaagataa aagcgatcgg gctactcagg agcaagtgct tgatgggcgt acacgactgg ttttggcggg attagtcaat cggggggtta tcggtatgat tgaacggtgt atcagtacag gtaaagaggc aaacgtctac tattcatccc ctaaccgtgc tgtcaaaatc taccgaacct ccatcctcgt cttccgtgct cgacaaaact acattgtagg cgaacaacgt ttccggggcg aatatacttc ttctcgaaat cctcgaaaga tgatccgtgt ctgggctgaa aaggagttgc gaaacctgag acggttggtt caaggaggtg tgcgtgcacc agtggtacat gaatgtaagg agaatgtgct cgtcatggac ttcttgggca agggcgaggt agcgtcgcct cgtttgaaag acgcagagat tcccgaagac aggttacccg atctgtacgc agaaatgatt attgcgactc gaagaatgta tcagcactgc cacctcgtcc acgccgattt gagtgaatac aacattctgt tacacgacaa ccacttatac atcatcgacg tctcgcaatc tgtcgaacac gaccacccgc gcgcattcga tttcctccgt tccgacatct ccaacattga agaattcttc tcccgccgtg gggtcgctac tctcggaatc cggaaatcat gggaattcat cgtcaccgag aacattggtc tttcccccac tggctccgcc tcgtcatcat tggagatgga gaagggtgat caaggcgaaa ggcgtttggt ggatgtcttg gaaaaatggt taaaagaacc gtcagacaag acggacgatg cggtctttat ggaatcgtat atcccgagaa cgttggcgga ggtgtatgat cccgagaggg atgtggatgt gctcaagagt gggtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat