CrneC.00830.a
Adenylyl-sulfate kinase (EC 2.7.1.25)
| CENTER ID: | CrneC.00830.a |
| ORGANISM: | Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487) |
| ASSOCIATED DISEASE: | Pneumonia-like illness |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrneC.00830.a.B1.GE41253 | full length | 1 | 203 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrneC.00830.a.B1.PS38292 | full length | 1 | 203 |
Structures
6B8V
DEPOSITED: 10/9/2017
DETERMINATION: XRay
CLONE: CrneC.00830.a.B1.GE41253
PROTEIN: CrneC.00830.a.B1.PS38292
6C6B
DEPOSITED: 1/18/2018
DETERMINATION: XRay
CLONE: CrneC.00830.a.B1.GE41253
PROTEIN: CrneC.00830.a.B1.PS38292
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:CNAG_02202 |
| UniProt: | J9VMZ3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATNITFHPG AVTQDERDTL LGQKGCTVWL TGLSASGKST IATALEQHLL HKKLHAYRLD GDNIRFGLNK DLGFDQASRV ENIRRIGEVS LLFALSSTIS VTAFISPYIS DRQLARELHE KHSSAIPFIE VFIDAPLSVV EQRDPKGLYK KARAGEIKDF TGISAPYEAP ANPEIHIRTD EVDVAGAVEI ITKYLADNGL IPA
NT Sequence
atggccacaa acatcacctt ccaccccgga gccgtcaccc aggacgagcg agataccctt ctcggccaga agggctgtac cgtctggctc actggtttga gcgcttccgg caagtctacc atcgcgacag cacttgaaca gcacttgctc cataaaaagc tccatgcgta taggttggac ggtgataaca tccgattcgg tctcaacaag gacctcggct ttgaccaggc ttcccgagtg gaaaacattc gacgaatcgg cgaagtctca ctcctctttg cactctcctc caccatctcc gtcacggcct tcatctcccc atacatctct gaccgacaac tcgcccgaga gttgcacgaa aaacactctt ccgccattcc cttcatcgaa gtcttcatcg acgctcccct ctccgtcgtc gagcagaggg accccaaggg cctgtacaaa aaggcaaggg ccggtgagat caaggacttt acaggtatct cggcgccgta tgaggctcct gcgaatcccg agatccacat caggaccgat gaggtggacg tggccggtgc agtcgagatc attacaaagt acctcgccga caatggtctt atccctgctt aa
Details for CrneC.00830.a.B1.GE41253
| HARVESTED ON: | 3/8/2017 |
| SEQUENCED ON: | 3/9/2017 |
| EXPECTED MW: | 23kDa |
| OBSERVED MW: | 23kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA TNITFHPGAV TQDERDTLLG QKGCTVWLTG LSASGKSTIA TALEQHLLHK KLHAYRLDGD NIRFGLNKDL GFDQASRVEN IRRIGEVSLL FALSSTISVT AFISPYISDR QLARELHEKH SSAIPFIEVF IDAPLSVVEQ RDPKGLYKKA RAGEIKDFTG ISAPYEAPAN PEIHIRTDEV DVAGAVEIIT KYLADNGLIP A
Validated NT Sequence
atggctcacc accaccacca ccatatggcc acaaacatca ccttccaccc cggagccgtc acccaggacg agcgagatac ccttctcggc cagaagggct gtaccgtctg gctcactggt ttgagcgctt ccggcaagtc taccatcgcg acagcacttg aacagcactt gctccataaa aagctccatg cgtataggtt ggacggtgat aacatccgat tcggtctcaa caaggacctc ggctttgacc aggcttcccg agtggaaaac attcgacgaa tcggcgaagt ctcactcctc tttgcactct cctccaccat ctccgtcacg gccttcatct ccccatacat ctctgaccga caactcgccc gagagttgca cgaaaaacac tcttccgcca ttcccttcat cgaagtcttc atcgacgctc ccctctccgt cgtcgagcag agggacccca agggcctgta caaaaaggca agggccggtg agatcaagga ctttacaggt atctcggcgc cgtatgaggc tcctgcgaat cccgagatcc acatcaggac cgatgaggtg gacgtggccg gtgcagtcga gatcattaca aagtacctcg ccgacaatgg tcttatccct gcttaagtga gtaagat
Expected Protein Sequence
MAHHHHHHMA TNITFHPGAV TQDERDTLLG QKGCTVWLTG LSASGKSTIA TALEQHLLHK KLHAYRLDGD NIRFGLNKDL GFDQASRVEN IRRIGEVSLL FALSSTISVT AFISPYISDR QLARELHEKH SSAIPFIEVF IDAPLSVVEQ RDPKGLYKKA RAGEIKDFTG ISAPYEAPAN PEIHIRTDEV DVAGAVEIIT KYLADNGLIP A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcca caaacatcac cttccacccc ggagccgtca cccaggacga gcgagatacc cttctcggcc agaagggctg taccgtctgg ctcactggtt tgagcgcttc cggcaagtct accatcgcga cagcacttga acagcacttg ctccataaaa agctccatgc gtataggttg gacggtgata acatccgatt cggtctcaac aaggacctcg gctttgacca ggcttcccga gtggaaaaca ttcgacgaat cggcgaagtc tcactcctct ttgcactctc ctccaccatc tccgtcacgg ccttcatctc cccatacatc tctgaccgac aactcgcccg agagttgcac gaaaaacact cttccgccat tcccttcatc gaagtcttca tcgacgctcc cctctccgtc gtcgagcaga gggaccccaa gggcctgtac aaaaaggcaa gggccggtga gatcaaggac tttacaggta tctcggcgcc gtatgaggct cctgcgaatc ccgagatcca catcaggacc gatgaggtgg acgtggccgg tgcagtcgag atcattacaa agtacctcgc cgacaatggt cttatccctg cttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for CrneC.00830.a.B1.PS38292
| PURIFICATION DATe: | 5/31/2017 |
| CONCENTRATION: | 55.9mg/ml |
| OBSERVED MW: | 26kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA TNITFHPGAV TQDERDTLLG QKGCTVWLTG LSASGKSTIA TALEQHLLHK KLHAYRLDGD NIRFGLNKDL GFDQASRVEN IRRIGEVSLL FALSSTISVT AFISPYISDR QLARELHEKH SSAIPFIEVF IDAPLSVVEQ RDPKGLYKKA RAGEIKDFTG ISAPYEAPAN PEIHIRTDEV DVAGAVEIIT KYLADNGLIP A
Validated NT Sequence
atggctcacc accaccacca ccatatggcc acaaacatca ccttccaccc cggagccgtc acccaggacg agcgagatac ccttctcggc cagaagggct gtaccgtctg gctcactggt ttgagcgctt ccggcaagtc taccatcgcg acagcacttg aacagcactt gctccataaa aagctccatg cgtataggtt ggacggtgat aacatccgat tcggtctcaa caaggacctc ggctttgacc aggcttcccg agtggaaaac attcgacgaa tcggcgaagt ctcactcctc tttgcactct cctccaccat ctccgtcacg gccttcatct ccccatacat ctctgaccga caactcgccc gagagttgca cgaaaaacac tcttccgcca ttcccttcat cgaagtcttc atcgacgctc ccctctccgt cgtcgagcag agggacccca agggcctgta caaaaaggca agggccggtg agatcaagga ctttacaggt atctcggcgc cgtatgaggc tcctgcgaat cccgagatcc acatcaggac cgatgaggtg gacgtggccg gtgcagtcga gatcattaca aagtacctcg ccgacaatgg tcttatccct gcttaagtga gtaagat
Expressed Protein Sequence
MAHHHHHHMA TNITFHPGAV TQDERDTLLG QKGCTVWLTG LSASGKSTIA TALEQHLLHK KLHAYRLDGD NIRFGLNKDL GFDQASRVEN IRRIGEVSLL FALSSTISVT AFISPYISDR QLARELHEKH SSAIPFIEVF IDAPLSVVEQ RDPKGLYKKA RAGEIKDFTG ISAPYEAPAN PEIHIRTDEV DVAGAVEIIT KYLADNGLIP A
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCA CAAACATCAC CTTCCACCCC GGAGCCGTCA CCCAGGACGA GCGAGATACC CTTCTCGGCC AGAAGGGCTG TACCGTCTGG CTCACTGGTT TGAGCGCTTC CGGCAAGTCT ACCATCGCGA CAGCACTTGA ACAGCACTTG CTCCATAAAA AGCTCCATGC GTATAGGTTG GACGGTGATA ACATCCGATT CGGTCTCAAC AAGGACCTCG GCTTTGACCA GGCTTCCCGA GTGGAAAACA TTCGACGAAT CGGCGAAGTC TCACTCCTCT TTGCACTCTC CTCCACCATC TCCGTCACGG CCTTCATCTC CCCATACATC TCTGACCGAC AACTCGCCCG AGAGTTGCAC GAAAAACACT CTTCCGCCAT TCCCTTCATC GAAGTCTTCA TCGACGCTCC CCTCTCCGTC GTCGAGCAGA GGGACCCCAA GGGCCTGTAC AAAAAGGCAA GGGCCGGTGA GATCAAGGAC TTTACAGGTA TCTCGGCGCC GTATGAGGCT CCTGCGAATC CCGAGATCCA CATCAGGACC GATGAGGTGG ACGTGGCCGG TGCAGTCGAG ATCATTACAA AGTACCTCGC CGACAATGGT CTTATCCCTG CTTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT