CrneC.00703.a

CAMK/CAMK1/CAMK1-RCK protein kinase

CENTER ID: CrneC.00703.a
ORGANISM: Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
ASSOCIATED DISEASE: Pneumonia-like illness
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
CrneC.00703.a.B2.GE41252 Truncation 1 106 424
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:CNAG_00130
UniProt: J9VDA1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLSSFKQILR HGKHAQQASP SPQPDTKSGR SKTTQTQPQQ PMPAVQQQAQ QPSQPQQQQQ HRRNDEYSSR LTTSTAQQNK VPAASPNYRE EAEKIVADER AQSEKMPVYA GLEEFKLIEK MGDGAFSNVY KAVDRRSGQK VAVKVVRKYE LNQSQHGNKH LNANFKKRGP RVTERANILK EVQIMRGIDH PSIVKLLKFF ESDEHYFLVL ELMEGGELFH QIVKLTYFSE ALSRHVILQV AEGIRYLHEE RGVVHRDIKP ENLLFERIPI IPSRNPVHRP YDEEKEDEGE FQPGIGGGEI GRVKIADFGL SKIVWDEQTM TPCGTVGYTA PEIVKDERYS KSVDMWALGC VLYTLLCGFP PFYDESINVL TEKVARGYYT FLSPWWDDIS HSAKDLISHL LCVDPAQRYT IDEFLAHPWI RDAAPPPPPP TSRPMPSESP MYSPLLASIR AGNREARSPG VGALKEAFDV TYAVHRMEEE GARRRAYNGP GGAGQRGFLH GLNEEAEEED EQAQVEEARR KHGEAVARQI QEHRGRAAAN AAAGIKEPPV TNYVGRGGAN RREAEAVIYD GRAGQRDRGK GGKSAGGFDL DLNNATLLGR RGKKVAPSPL AQQVQAGQ
NT Sequence
atgctgtcaa gtttcaaaca gatccttcgt cacgggaagc acgctcaaca ggcgtctccg agcccccagc ccgatacgaa gagcggccga tcaaagacca ctcaaactca gcctcagcag ccgatgcctg ctgttcaaca acaagcgcaa cagccatccc agccacagca acaacaacaa catcgccgca atgatgaata cagctcaagg cttacaacga gcactgctca acaaaacaaa gtacccgccg cttcacccaa ctacagagaa gaagccgaaa aaatcgttgc cgatgagagg gcacaaagcg aaaagatgcc agtatatgct ggactcgaag agttcaagtt gatagaaaag atgggcgacg gtgccttctc taatgtgtac aaagccgttg accgcaggag tggtcagaaa gtggccgtga aggttgttcg caagtatgaa ctcaatcagt cacagcacgg taacaagcat ctgaacgcca acttcaagaa gaggggaccg agggtcacag agcgtgccaa tattcttaag gaagtccaga tcatgcgcgg cattgaccat cctagtatcg tcaagctttt gaaattcttc gaaagcgacg aacattactt cctggtcctc gaattgatgg agggcggtga attattccat caaatcgtca agctcaccta cttttctgag gctctttccc gtcacgttat cctgcaagtc gctgaaggta ttcgatattt gcacgaggag cgaggtgttg tccaccgtga tattaagcca gaaaatctct tgttcgaacg catcccaatt atcccctccc gtaatcccgt acaccgaccg tatgatgagg agaaggaaga tgaaggagaa ttccagcctg gaattggggg tggtgagatc ggtagggtga aaattgccga ctttggactg tcaaagattg tatgggatga gcagaccatg accccttgcg gtaccgtcgg atacactgct cccgagatcg tcaaagacga aagatatagt aagagcgtcg acatgtgggc ccttggttgt gtcttataca ctcttctgtg tggtttccct cccttctacg acgaaagcat caacgttctt acagaaaaag tcgctcgagg ttactacacc ttcttaagtc cctggtggga cgatatttct cattccgcca aagacctcat ttcccatctc ctttgcgttg accctgctca gcgatatacc attgatgagt tcctcgccca tccttggatc cgagatgctg ccccgccgcc tcctcctccc acttctcgcc ctatgcccag tgaatctccc atgtactctc ctctccttgc ttctattcgc gctggtaacc gtgaggcccg ctctcctgga gtgggtgccc tcaaggaagc ctttgacgtt acttacgctg ttcatcgcat ggaagaagag ggcgctcgac gccgtgcgta caatggtccc ggcggcgctg gtcagcgtgg gttcttgcat ggcctgaatg aggaggcaga agaagaagat gagcaagcgc aggttgaaga agctcgtagg aagcacgggg aagctgtggc ccgtcaaatc caagaacatc gtggtcgtgc agctgccaat gccgctgctg gcatcaaaga gccacctgtg accaactacg tcggtcgtgg aggtgccaac aggagagaag ccgaagcagt gatttacgat ggccgagcag ggcaaaggga taggggcaag ggtggcaaaa gtgctggagg gttcgacctg gacctcaata acgcaacatt acttggcagg agaggaaaga aggttgcccc cagtcctcta gcacagcagg tgcaagccgg ccaataa
Details for CrneC.00703.a.B2.GE41252
HARVESTED ON: 3/8/2017
SEQUENCED ON: 3/9/2017
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP VYAGLEEFKL IEKMGDGAFS NVYKAVDRRS GQKVAVKVVR KYELNQSQHG NKHLNANFKK RGPRVTERAN ILKEVQIMRG IDHPSIVKLL KFFESDEHYF LVLELMEGGE LFHQIVKLTY FSEALSRHVI LQVAEGIRYL HEERGVVHRD IKPENLLFER IPIIPSRNPV HRPYDEEKED EGEFQPGIGG GEIGRVKIAD FGLSKIVWDE QTMTPCGTVG YTAPEIVKDE RYSKSVDMWA LGCVLYTLLC VFPPFYDESI NVLTEKVARG YYTFLSPWWD DISHSAKDLI SHLLCVDPAQ RYTIDEFLAX XXIRDAA
Validated NT Sequence
agatatacca tggctcacca ccaccaccac catatgccag tatatgctgg actcgaagag ttcaagttga tagaaaagat gggcgacggt gccttctcta atgtgtacaa agccgttgac cgcaggagtg gtcagaaagt ggccgtgaag gttgttcgca agtatgaact caatcagtca cagcacggta acaagcatct gaacgccaac ttcaagaaga ggggaccgag ggtcacagag cgtgccaata ttcttaagga agtccagatc atgcgcggca ttgaccatcc tagtatcgtc aagcttttga aattcttcga aagcgacgaa cattacttcc tggtcctcga attgatggag ggcggtgaat tattccatca aatcgtcaag ctcacctact tttctgaggc tctttcccgt cacgttatcc tgcaagtcgc tgaaggtatt cgatatttgc acgaggagcg aggtgttgtc caccgtgata ttaagccaga aaatctcttg ttcgaacgca tcccaattat cccctcccgt aatcccgtac accgaccgta tgatgaggag aaggaagatg aaggagaatt ccagcctgga attgggggtg gtgagatcgg tagggtgaaa attgccgact ttggactgtc aaagattgta tgggatgagc agaccatgac cccttgcggt accgtcggat acactgctcc cgagatcgtc aaagacgaaa gatatagtaa gagcgtcgac atgtgggccc ttggttgtgt cttatacact cttctgtgtg ttttccctcc cttctacgac gaaagcatca acgttcttac agaaaaagtc gctcgaggtt actacacctt cttaagtccc tggtgggacg atatttctca ttccgccaaa gacctcattt cccatctcct ttgcgttgac cctgctcagc gatataccat tgatgagttc ctcgcnnnnn nnnnnatccg agatgctgcc taagtgag
Expected Protein Sequence
MAHHHHHHMP VYAGLEEFKL IEKMGDGAFS NVYKAVDRRS GQKVAVKVVR KYELNQSQHG NKHLNANFKK RGPRVTERAN ILKEVQIMRG IDHPSIVKLL KFFESDEHYF LVLELMEGGE LFHQIVKLTY FSEALSRHVI LQVAEGIRYL HEERGVVHRD IKPENLLFER IPIIPSRNPV HRPYDEEKED EGEFQPGIGG GEIGRVKIAD FGLSKIVWDE QTMTPCGTVG YTAPEIVKDE RYSKSVDMWA LGCVLYTLLC GFPPFYDESI NVLTEKVARG YYTFLSPWWD DISHSAKDLI SHLLCVDPAQ RYTIDEFLAH PWIRDAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccag tatatgctgg actcgaagag ttcaagttga tagaaaagat gggcgacggt gccttctcta atgtgtacaa agccgttgac cgcaggagtg gtcagaaagt ggccgtgaag gttgttcgca agtatgaact caatcagtca cagcacggta acaagcatct gaacgccaac ttcaagaaga ggggaccgag ggtcacagag cgtgccaata ttcttaagga agtccagatc atgcgcggca ttgaccatcc tagtatcgtc aagcttttga aattcttcga aagcgacgaa cattacttcc tggtcctcga attgatggag ggcggtgaat tattccatca aatcgtcaag ctcacctact tttctgaggc tctttcccgt cacgttatcc tgcaagtcgc tgaaggtatt cgatatttgc acgaggagcg aggtgttgtc caccgtgata ttaagccaga aaatctcttg ttcgaacgca tcccaattat cccctcccgt aatcccgtac accgaccgta tgatgaggag aaggaagatg aaggagaatt ccagcctgga attgggggtg gtgagatcgg tagggtgaaa attgccgact ttggactgtc aaagattgta tgggatgagc agaccatgac cccttgcggt accgtcggat acactgctcc cgagatcgtc aaagacgaaa gatatagtaa gagcgtcgac atgtgggccc ttggttgtgt cttatacact cttctgtgtg gtttccctcc cttctacgac gaaagcatca acgttcttac agaaaaagtc gctcgaggtt actacacctt cttaagtccc tggtgggacg atatttctca ttccgccaaa gacctcattt cccatctcct ttgcgttgac cctgctcagc gatataccat tgatgagttc ctcgcccatc cttggatccg agatgctgcc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat