CrneC.00629.a
Acetyl-coenzyme A synthetase (EC 6.2.1.1)
| CENTER ID: | CrneC.00629.a |
| ORGANISM: | Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487) |
| ASSOCIATED DISEASE: | Pneumonia-like illness |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrneC.00629.a.FS11.GE00458 | full length | 3 | 681 | ||
| CrneC.00629.a.HA11.GE00478 | full length | 46 | 679 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| CrneC.00629.a.FS11.PD00402 | full length | 3 | 681 | ||
| CrneC.00629.a.HA11.PD00407 | full length | 46 | 679 | ||
| CrneC.00629.a.FS11.PD00460 | full length | 3 | 681 |
Structures
5IFI
DEPOSITED: 2/26/2016
DETERMINATION: XRay
CLONE: CrneC.00629.a.FS11.GE00458
PROTEIN: CrneC.00629.a.FS11.PD00402
5K85
DEPOSITED: 5/27/2016
DETERMINATION: XRay
CLONE: CrneC.00629.a.FS11.GE00458
PROTEIN: CrneC.00629.a.FS11.PD00402
5K8F
DEPOSITED: 5/30/2016
DETERMINATION: XRay
CLONE: CrneC.00629.a.FS11.GE00458
PROTEIN: CrneC.00629.a.FS11.PD00402
5U29
DEPOSITED: 11/30/2016
DETERMINATION: XRay
CLONE: CrneC.00629.a.FS11.GE00458
PROTEIN: CrneC.00629.a.FS11.PD00402
5VPV
DEPOSITED: 5/5/2017
DETERMINATION: XRay
CLONE: CrneC.00629.a.FS11.GE00458
PROTEIN: CrneC.00629.a.FS11.PD00402
7KNO
DEPOSITED: 11/5/2020
DETERMINATION: XRay
CLONE: CrneC.00629.a.FS11.GE00458
PROTEIN: CrneC.00629.a.FS11.PD00402
7KNP
DEPOSITED: 11/5/2020
DETERMINATION: XRay
CLONE: CrneC.00629.a.FS11.GE00458
PROTEIN: CrneC.00629.a.FS11.PD00460
7L4G
DEPOSITED: 12/19/2020
DETERMINATION: XRay
CLONE: CrneC.00629.a.FS11.GE00458
PROTEIN: CrneC.00629.a.FS11.PD00460
8EPS
DEPOSITED: 10/6/2022
DETERMINATION: XRay
CLONE: CrneC.00629.a.FS11.GE00458
PROTEIN: CrneC.00629.a.FS11.PD00460
8G0R
DEPOSITED: 2/1/2023
DETERMINATION: XRay
CLONE: CrneC.00629.a.FS11.GE00458
PROTEIN: CrneC.00629.a.FS11.PD00460
8G0S
DEPOSITED: 2/1/2023
DETERMINATION: XRay
CLONE: CrneC.00629.a.FS11.GE00458
PROTEIN: CrneC.00629.a.FS11.PD00460
8G0T
DEPOSITED: 2/1/2023
DETERMINATION: XRay
CLONE: CrneC.00629.a.FS11.GE00458
PROTEIN: CrneC.00629.a.FS11.PD00460
8G0U
DEPOSITED: 2/1/2023
DETERMINATION: XRay
CLONE: CrneC.00629.a.FS11.GE00458
PROTEIN: CrneC.00629.a.FS11.PD00460
8G0V
DEPOSITED: 2/1/2023
DETERMINATION: XRay
CLONE: CrneC.00629.a.FS11.GE00458
PROTEIN: CrneC.00629.a.FS11.PD00460
8V5G
DEPOSITED: 11/30/2023
DETERMINATION: XRay
CLONE: CrneC.00629.a.FS11.GE00458
PROTEIN: CrneC.00629.a.FS11.PD00460
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:CNAG_00797 |
| RefSeq: | XP_012046967.1 |
| UniProt: | J9VFT1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKTEVAPGV HHVHPLPDSV PESEDLFAPP PRMQGKEGRP KPHIGPNYES YVKEWAKTVG PNSDEWWAAK ARETLDWYDD FKTVRAGGFE HGDVQWFPEG TLNAAYNCLD RHYYKNPKKT AIIYEADEPS ESREVSYEEL MQETCRVANV LKSYGVKKGD AVSIYLPMTW QAAAAFLACA RIGAIHSAVF AGFSAESLRD RVNDCECKVL ITTDEGRRGG KTIATKQIVD AALQQCPLVE NVLVLRRTGN KVPMTEGRDK WWDEECAKMP AYCPCERMAS EDPLFILYTS GSTGKPKGVV HSTAGYLLGT ALTLKYVFDA HPDDRFACMA DIGWITGHSY IIYGPLANGI TTAVFESTPV YPTPSRYWDF VDKWKATQLY TAPTAIRLLR RMGEDHVKNH DLSSLRVLGS VGEPINPEAW HWYNDFAGKN QCAIVDTYWM TETGSISIAP LPGAISTKPG SATFPFFGMD VDIIDPQTGQ VLEGNDVEGV LVARRPWPSI ARTVYRDHKR YLETYMKPYP GYFFFGDGAA RDYDGYMWIK GRVDDVINVS GHRLSTAEVE SALILHKGVA ETAVVGCADD LTGQAVYAFV TMKPEFDLKA TKEADLSKEL AIQVRKVIGP FAAPKKIYLV SDLPKTRSGK IMRRVLRKIV AGEGDQLGDL SSIADPQIVE EVKQKVTGSA
NT Sequence
atgggcaaga cagaagtcgc acccggcgtc caccacgtcc accctctccc cgactccgtc cccgagtctg aggacctctt cgctccccct ccccgaatgc aggggaagga aggccgcccg aagcctcaca tcggtcctaa ctacgagtcc tacgtcaagg aatgggccaa gactgttggt cccaatagtg atgagtggtg ggccgcaaag gccagggaaa ctcttgattg gtacgacgac tttaagactg tcagggccgg tggctttgag catggcgacg ttcagtggtt ccccgaaggt accttgaacg ccgcctacaa ctgtcttgac cgacactact acaagaaccc caaaaagacc gccatcatct acgaggctga cgagccttcc gagtcccgtg aggtctctta cgaggagctc atgcaggaga cttgccgagt tgccaacgtc ctcaagagct acggtgtgaa gaagggtgac gctgtgtcca tctatcttcc catgacttgg caagccgctg ctgcgttcct tgcttgtgcc agaatcggtg ccatccactc tgctgtcttt gccggtttta gtgccgagag tttgagggat agggtcaatg attgtgaatg caaggttttg atcaccactg acgagggtcg acgaggtggt aagactattg ctaccaagca gatcgtcgat gccgctctcc agcaatgccc ccttgttgag aacgttctcg ttcttcgacg aaccggcaac aaggtcccca tgactgaggg ccgtgacaaa tggtgggacg aagagtgtgc caagatgccc gcttactgtc cttgcgagag gatggcttcc gaggatcctc tcttcatcct ctacacctct ggttccaccg gtaagccgaa gggtgttgtc cactccactg ctggttacct cctcggcacc gcccttaccc tcaagtacgt ctttgacgct catcctgatg accgattcgc ctgtatggcc gatatcggat ggattacggg tcacagttac atcatctacg gccctctcgc caacggcatc accaccgccg tctttgaatc cacccccgtc tatcccacac catccagata ctgggacttt gttgacaagt ggaaagccac tcagctctac actgcgccca ctgcgatccg attgttaaga cgtatgggcg aggaccacgt gaagaaccac gatctcagct ctctccgagt tttgggctct gttggtgagc ccatcaaccc cgaggcttgg cattggtaca atgactttgc gggcaagaat caatgtgcca ttgttgacac atactggatg accgagactg gttctatttc tattgctcct ctccctggag ccatctccac caagcccggt tctgccactt tccccttctt cggtatggat gttgacatca ttgaccctca aactggtcag gtcttggagg gtaacgatgt agagggtgtt ctcgttgcca ggaggccatg gcccagtatt gccaggactg tctacaggga ccacaagagg tatcttgaga cttacatgaa gccttacccc ggctacttct tctttggtga tggtgctgcc cgagactatg atggttacat gtggatcaag ggacgagttg atgatgttat caacgtgtct ggtcaccgac tttccacggc cgaggtcgag tctgccctta tccttcacaa gggtgtcgcc gagactgccg ttgttggatg cgctgatgac cttactggtc aggctgttta cgctttcgtt accatgaagc ccgagtttga tctaaaggct accaaggagg ccgaccttag caaggagttg gctatccagg ttaggaaggt tattggtccc tttgccgctc ccaagaagat ttaccttgtt tccgatctcc ccaagacccg atcaggaaag atcatgcgtc gagttctccg aaagattgtc gctggcgagg gtgaccaatt gggagatctt tcttccatcg ctgaccccca aattgtcgaa gaagtcaagc aaaaggttac cggctctgct taa
Details for CrneC.00629.a.FS11.GE00458
| HARVESTED ON: | 8/7/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 77kDa |
| OBSERVED MW: | 77kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | Data not available |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MHHHHHHHHE NLYFQGKTEV APGVHHVHPL PDSVPESEDL FAPPPRMQGK EGRPKPHIGP NYESYVKEWA KTVGPNSDEW WAAKARETLD WYDDFKTVRA GGFEHGDVQW FPEGTLNAAY NCLDRHYYKN PKKTAIIYEA DEPSESREVS YEELMQETCR VANVLKSYGV KKGDAVSIYL PMTWQAAAAF LACARIGAIH SAVFAGFSAE SLRDRVNDCE CKVLITTDEG RRGGKTIATK QIVDAALQQC PLVENVLVLR RTGNKVPMTE GRDKWWDEEC AKMPAYCPCE RMASEDPLFI LYTSGSTGKP KGVVHSTAGY LLGTALTLKY VFDAHPDDRF ACMADIGWIT GHSYIIYGPL ANGITTAVFE STPVYPTPSR YWDFVDKWKA TQLYTAPTAI RLLRRMGEDH VKNHDLSSLR VLGSVGEPIN PEAWHWYNDF AGKNQCAIVD TYWMTETGSI SIAPLPGAIS TKPGSATFPF FGMDVDIIDP QTGQVLEGND VEGVLVARRP WPSIARTVYR DHKRYLETYM KPYPGYFFFG DGAARDYDGY MWIKGRVDDV INVSGHRLST AEVESALILH KGVAETAVVG CADDLTGQAV YAFVTMKPEF DLKATKEADL SKELAIQVRK VIGPFAAPKK IYLVSDLPKT RSGKIMRRVL RKIVAGEGDQ LGDLSSIADP QIVEEVKQKV TGSA
Full NT Sequence (Expression Vector + Insert)
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aacttttaga gaccaaggag gtaaaaaatg catcaccacc accatcacca ccacgaaaat ctgtacttcc aaggtaagac cgaagtcgca ccgggtgttc accacgttca cccactgccg gattcagttc cggaatccga ggacctgttt gcaccgcctc cgcgtatgca aggcaaagag ggccgtccga aaccgcacat tggcccgaac tacgagagct acgtcaagga atgggctaaa acggtcggcc cgaacagcga tgagtggtgg gcagcgaaag ctcgcgaaac cctggattgg tatgacgact ttaagaccgt gcgtgccggt ggcttcgagc acggtgacgt gcaatggttc cctgagggta ccctgaatgc cgcgtacaat tgtttggatc gtcactatta caagaatccg aagaaaaccg cgatcattta cgaagcggat gagccgagcg agagccgcga agtgtcctat gaagaactga tgcaagaaac ctgtcgtgtt gcgaacgttc tgaaaagcta tggcgtcaag aaaggtgacg cagtgtctat ctatctcccg atgacctggc aggcagccgc ggcgtttctg gcctgtgcgc gcattggtgc gatccattcg gcagtgttcg ctggcttcag cgcggagagc ctgcgcgacc gtgtcaatga ctgcgagtgc aaagtgctga tcaccaccga tgagggccgt cgcggtggca aaacgatcgc aacgaaacag atcgtcgatg cggcactgca gcagtgcccg ttggttgaaa atgtgttggt tctgcgtcgc acgggtaaca aagttccgat gaccgagggt cgtgacaagt ggtgggatga agagtgcgcg aaaatgccgg cgtattgccc gtgtgagcgt atggcttccg aggacccgct gtttattctg tacaccagcg gtagcactgg caagccgaaa ggtgtggtgc atagcaccgc tggttatctg ctgggtaccg cgctgaccct gaaatatgtt tttgatgcgc atccggacga ccgtttcgcc tgcatggcag atattggttg gattactggc cacagctaca ttatctacgg ccctctggcc aacggtatca cgacggcagt ctttgagagc accccggtgt atccgacgcc gagccgttat tgggacttcg tcgataaatg gaaagcgacg cagctgtaca ccgccccaac ggcgatccgt ttattgcgcc gcatgggtga ggaccatgtt aagaatcacg atctgagctc tctgcgtgtt ttgggtagcg tcggcgagcc gatcaaccca gaggcgtggc attggtacaa cgatttcgca ggcaaaaatc agtgtgcaat tgtggacacc tactggatga cggaaacggg ctctatcagc attgccccgc tgcctggcgc gattagcacc aaaccgggtt cggcgacttt tccgtttttc ggtatggacg tcgacattat tgacccgcag actggccaag ttcttgaggg taacgacgtc gaaggcgtgc tggttgctcg ccgtccgtgg ccgagcatcg cgcgcacggt ctaccgtgac cataagcgct acctggaaac ctatatgaag ccgtacccgg gttacttctt cttcggcgac ggtgcggcac gtgactatga tggttacatg tggattaagg gtcgtgtcga tgatgttatc aacgtgagcg gtcaccgtct gagcaccgca gaagtggagt ccgcgctgat tctgcataag ggtgtcgctg aaaccgctgt cgtcggttgc gcggacgatc tgacgggcca agccgtttac gcctttgtga ccatgaagcc agagttcgat ctgaaggcca ccaaggaagc tgatctgagc aaagaactgg caatccaggt gcgcaaggtt atcggtccgt ttgcagcgcc gaagaaaatc tatctggtta gcgatttgcc aaagactcgt tccggcaaga ttatgcgtcg tgtgctgaga aaaatcgtgg cgggtgaggg tgaccaactg ggtgatttga gcagcattgc ggacccgcag attgttgagg aagttaagca aaaagttacc ggcagcgcct aatgaggttg aggtctcacc ccctagcata accccttggg gcctctaaac gggtcttgag gggttttttg cccctgagac gcgtcaatcg agttcgtacc taagggcgac accccctaat tagcccgggc gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg gagtccccac actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca aacaaggggt gttatgagcc atattcaggt ataaatgggc tcgcgataat gttcagaatt ggttaattgg ttgtaacact gacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagaat atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gccacttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cgttccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcac tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgcct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa gcggcgcgcc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atatgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca tgaggacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagatagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc cagtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtga
Details for CrneC.00629.a.HA11.GE00478
| HARVESTED ON: | 8/5/2015 |
| SEQUENCED ON: | 5/4/2017 |
| EXPECTED MW: | 73kDa |
| OBSERVED MW: | 73kDa |
| ANTIBIOTIC MARKER: | kanamycin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MHHHHHHHHE NLYFQGPNYE SYVKEWAKTV GPNSDEWWAA KARETLDWYD DFKTVRAGGF EHGDVQWFPE GTLNAAYNCL DRHYYKNPKK TAIIYEADEP SESREVSYEE LMQETCRVAN VLKSYGVKKG DAVSIYLPMT WQAAAAFLAC ARIGAIHSAV FAGFSAESLR DRVNDCECKV LITTDEGRRG GKTIATKQIV DAALQQCPLV ENVLVLRRTG NKVPMTEGRD KWWDEECAKM PAYCPCERMA SEDPLFILYT SGSTGKPKGV VHSTAGYLLG TALTLKYVFD AHPDDRFACM ADIGWITGHS YIIYGPLANG ITTAVFESTP VYPTPSRYWD FVDKWKATQL YTAPTAIRLL RRMGEDHVKN HDLSSLRVLG SVGEPINPEA WHWYNDFAGK NQCAIVDTYW MTETGSISIA PLPGAISTKP GSATFPFFGM DVDIIDPQTG QVLEGNDVEG VLVARRPWPS IARTVYRDHK RYLETYMKPY PGYFFFGDGA ARDYDGYMWI KGRVDDVINV SGHRLSTAEV ESALILHKGV AETAVVGCAD DLTGQAVYAF VTMKPEFDLK ATKEADLSKE LAIQVRKVIG PFAAPKKIYL VSDLPKTRSG KIMRRVLRKI VAGEGDQLGD LSSIADPQIV EEVKQKVTG
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgcaccatc accaccacca tcatcacgag aatctgtact tccaaggccc gaactacgag agctacgtca aggaatgggc taaaacggtc ggcccgaaca gcgatgagtg gtgggcagcg aaagctcgcg aaaccctgga ttggtatgac gactttaaga ccgtgcgtgc cggtggcttc gagcacggtg acgtgcaatg gttccctgag ggtaccctga atgccgcgta caattgtttg gatcgtcact attacaagaa tccgaagaaa accgcgatca tttacgaagc ggatgagccg agcgagagcc gcgaagtgtc ctatgaagaa ctgatgcaag aaacctgtcg tgttgcgaac gttctgaaaa gctatggcgt caagaaaggt gacgcagtgt ctatctatct cccgatgacc tggcaggcag ccgcggcgtt tctggcctgt gcgcgcattg gtgcgatcca ttcggcagtg ttcgctggct tcagcgcgga gagcctgcgc gaccgtgtca atgactgcga gtgcaaagtg ctgatcacca ccgatgaggg ccgtcgcggt ggcaaaacga tcgcaacgaa acagatcgtc gatgcggcac tgcagcagtg cccgttggtt gaaaatgtgt tggttctgcg tcgcacgggt aacaaagttc cgatgaccga gggtcgtgac aagtggtggg atgaagagtg cgcgaaaatg ccggcgtatt gcccgtgtga gcgtatggct tccgaggacc cgctgtttat tctgtacacc agcggtagca ctggcaagcc gaaaggtgtg gtgcatagca ccgctggtta tctgctgggt accgcgctga ccctgaaata tgtttttgat gcgcatccgg acgaccgttt cgcctgcatg gcagatattg gttggattac tggccacagc tacattatct acggccctct ggccaacggt atcacgacgg cagtctttga gagcaccccg gtgtatccga cgccgagccg ttattgggac ttcgtcgata aatggaaagc gacgcagctg tacaccgccc caacggcgat ccgtttattg cgccgcatgg gtgaggacca tgttaagaat cacgatctga gctctctgcg tgttttgggt agcgtcggcg agccgatcaa cccagaggcg tggcattggt acaacgattt cgcaggcaaa aatcagtgtg caattgtgga cacctactgg atgacggaaa cgggctctat cagcattgcc ccgctgcctg gcgcgattag caccaaaccg ggttcggcga cttttccgtt tttcggtatg gacgtcgaca ttattgaccc gcagactggc caagttcttg agggtaacga cgtcgaaggc gtgctggttg ctcgccgtcc gtggccgagc atcgcgcgca cggtctaccg tgaccataag cgctacctgg aaacctatat gaagccgtac ccgggttact tcttcttcgg cgacggtgcg gcacgtgact atgatggtta catgtggatt aagggtcgtg tcgatgatgt tatcaacgtg agcggtcacc gtctgagcac cgcagaagtg gagtccgcgc tgattctgca taagggtgtc gctgaaaccg ctgtcgtcgg ttgcgcggac gatctgacgg gccaagccgt ttacgccttt gtgaccatga agccagagtt cgatctgaag gccaccaagg aagctgatct gagcaaagaa ctggcaatcc aggtgcgcaa ggttatcggt ccgtttgcag cgccgaagaa aatctatctg gttagcgatt tgccaaagac tcgttccggc aagattatgc gtcgtgtgct gagaaaaatc gtggcgggtg agggtgacca actgggtgat ttgagcagca ttgcggaccc gcagattgtt gaggaagtta agcaaaaagt taccggctga taagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat
Details for CrneC.00629.a.FS11.PD00402
| PURIFICATION DATe: | 10/2/2015 |
| CONCENTRATION: | 20.48mg/ml |
| OBSERVED MW: | 77kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM Tris, pH = 8.0, 200 mM NaCl, 1 mM TCEP, 1% glycerol |
| EXPRESSION HOST: | Data not available |
| VIAL COUNT (approx.): | 110 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MHHHHHHHHE NLYFQGKTEV APGVHHVHPL PDSVPESEDL FAPPPRMQGK EGRPKPHIGP NYESYVKEWA KTVGPNSDEW WAAKARETLD WYDDFKTVRA GGFEHGDVQW FPEGTLNAAY NCLDRHYYKN PKKTAIIYEA DEPSESREVS YEELMQETCR VANVLKSYGV KKGDAVSIYL PMTWQAAAAF LACARIGAIH SAVFAGFSAE SLRDRVNDCE CKVLITTDEG RRGGKTIATK QIVDAALQQC PLVENVLVLR RTGNKVPMTE GRDKWWDEEC AKMPAYCPCE RMASEDPLFI LYTSGSTGKP KGVVHSTAGY LLGTALTLKY VFDAHPDDRF ACMADIGWIT GHSYIIYGPL ANGITTAVFE STPVYPTPSR YWDFVDKWKA TQLYTAPTAI RLLRRMGEDH VKNHDLSSLR VLGSVGEPIN PEAWHWYNDF AGKNQCAIVD TYWMTETGSI SIAPLPGAIS TKPGSATFPF FGMDVDIIDP QTGQVLEGND VEGVLVARRP WPSIARTVYR DHKRYLETYM KPYPGYFFFG DGAARDYDGY MWIKGRVDDV INVSGHRLST AEVESALILH KGVAETAVVG CADDLTGQAV YAFVTMKPEF DLKATKEADL SKELAIQVRK VIGPFAAPKK IYLVSDLPKT RSGKIMRRVL RKIVAGEGDQ LGDLSSIADP QIVEEVKQKV TGSA
Full NT Sequence (Expression Vector + Insert)
TTAATAAGAT GATCTTCTTG AGATCGTTTT GGTCTGCGCG TAATCTCTTG CTCTGAAAAC GAAAAAACCG CCTTGCAGGG CGGTTTTTCG AAGGTTCTCT GAGCTACCAA CTCTTTGAAC CGAGGTAACT GGCTTGGAGG AGCGCAGTCA CCAAAACTTG TCCTTTCAGT TTAGCCTTAA CCGGCGCATG ACTTCAAGAC TAACTCCTCT AAATCAATTA CCAGTGGCTG CTGCCAGTGG TGCTTTTGCA TGTCTTTCCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGACTGA ACGGGGGGTT CGTGCATACA GTCCAGCTTG GAGCGAACTG CCTACCCGGA ACTGAGTGTC AGGCGTGGAA TGAGACAAAC GCGGCCATAA CAGCGGAATG ACACCGGTAA ACCGAAAGGC AGGAACAGGA GAGCGCACGA GGGAGCCGCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCACT GATTTGAGCG TCAGATTTCG TGATGCTTGT CAGGGGGGCG GAGCCTATGG AAAAACGGCT TTGCCGCGGC CCTCTCACTT CCCTGTTAAG TATCTTCCTG GCATCTTCCA GGAAATCTCC GCCCCGTTCG TAAGCCATTT CCGCTCGCCG CAGTCGAACG ACCGAGCGTA GCGAGTCAGT GAGCGAGGAA GCGGAATATA TCCTGTATCA CATATTCTGC TGACGCACCG GTGCAGCCTT TTTTCTCCTG CCACATGAAG CACTTCACTG ACACCCTCAT CAGTGCCAAC ATAGTAAGCC AGTATACACT CCGCTAGCGC TGAGGTCCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGGCG AGAGTAGGGA ACTGCCAGGC ATCAAACTAA GCAGAAGGCC CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTCT GTGTTGTAAA ACGACGGCCA GTCTTAAGCT CGGGCCCCCT GGGCGGTTCT GATAACGAGT AATCGTTAAT CCGCAAATAA CGTAAAAACC CGCTTCGGCG GGTTTTTTTA TGGGGGGAGT TTAGGGAAAG AGCATTTGTC AGAATATTTA AGGGCGCCTG TCACTTTGCT TGATATATGA GAATTATTTA ACCTTATAAA TGAGAAAAAA GCAACGCACT TTAAATAAGA TACGTTGCTT TTTCGATTGA TGAACACCTA TAATTAAACT ATTCATCTAT TATTTATGAT TTTTTGTATA TACAATATTT CTAGTTTGTT AAAGAGAATT AAGAAAATAA ATCTCGAAAA TAATAAAGGG AAAATCAGTT TTTGATATCA AAATTATACA TGTCAACGAT AATACAAAAT ATAATACAAA CTATAAGATG TTATCAGTAT TTATTATGCA TTTAGAATAA ATTTTGTGTC GCCCTTCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC CTCTAGAAAT AATTTTGTTT AACTTTTAGA GACCAAGGAG GTAAAAAATG CATCACCACC ACCATCACCA CCACGAAAAT CTGTACTTCC AAGGTAAGAC CGAAGTCGCA CCGGGTGTTC ACCACGTTCA CCCACTGCCG GATTCAGTTC CGGAATCCGA GGACCTGTTT GCACCGCCTC CGCGTATGCA AGGCAAAGAG GGCCGTCCGA AACCGCACAT TGGCCCGAAC TACGAGAGCT ACGTCAAGGA ATGGGCTAAA ACGGTCGGCC CGAACAGCGA TGAGTGGTGG GCAGCGAAAG CTCGCGAAAC CCTGGATTGG TATGACGACT TTAAGACCGT GCGTGCCGGT GGCTTCGAGC ACGGTGACGT GCAATGGTTC CCTGAGGGTA CCCTGAATGC CGCGTACAAT TGTTTGGATC GTCACTATTA CAAGAATCCG AAGAAAACCG CGATCATTTA CGAAGCGGAT GAGCCGAGCG AGAGCCGCGA AGTGTCCTAT GAAGAACTGA TGCAAGAAAC CTGTCGTGTT GCGAACGTTC TGAAAAGCTA TGGCGTCAAG AAAGGTGACG CAGTGTCTAT CTATCTCCCG ATGACCTGGC AGGCAGCCGC GGCGTTTCTG GCCTGTGCGC GCATTGGTGC GATCCATTCG GCAGTGTTCG CTGGCTTCAG CGCGGAGAGC CTGCGCGACC GTGTCAATGA CTGCGAGTGC AAAGTGCTGA TCACCACCGA TGAGGGCCGT CGCGGTGGCA AAACGATCGC AACGAAACAG ATCGTCGATG CGGCACTGCA GCAGTGCCCG TTGGTTGAAA ATGTGTTGGT TCTGCGTCGC ACGGGTAACA AAGTTCCGAT GACCGAGGGT CGTGACAAGT GGTGGGATGA AGAGTGCGCG AAAATGCCGG CGTATTGCCC GTGTGAGCGT ATGGCTTCCG AGGACCCGCT GTTTATTCTG TACACCAGCG GTAGCACTGG CAAGCCGAAA GGTGTGGTGC ATAGCACCGC TGGTTATCTG CTGGGTACCG CGCTGACCCT GAAATATGTT TTTGATGCGC ATCCGGACGA CCGTTTCGCC TGCATGGCAG ATATTGGTTG GATTACTGGC CACAGCTACA TTATCTACGG CCCTCTGGCC AACGGTATCA CGACGGCAGT CTTTGAGAGC ACCCCGGTGT ATCCGACGCC GAGCCGTTAT TGGGACTTCG TCGATAAATG GAAAGCGACG CAGCTGTACA CCGCCCCAAC GGCGATCCGT TTATTGCGCC GCATGGGTGA GGACCATGTT AAGAATCACG ATCTGAGCTC TCTGCGTGTT TTGGGTAGCG TCGGCGAGCC GATCAACCCA GAGGCGTGGC ATTGGTACAA CGATTTCGCA GGCAAAAATC AGTGTGCAAT TGTGGACACC TACTGGATGA CGGAAACGGG CTCTATCAGC ATTGCCCCGC TGCCTGGCGC GATTAGCACC AAACCGGGTT CGGCGACTTT TCCGTTTTTC GGTATGGACG TCGACATTAT TGACCCGCAG ACTGGCCAAG TTCTTGAGGG TAACGACGTC GAAGGCGTGC TGGTTGCTCG CCGTCCGTGG CCGAGCATCG CGCGCACGGT CTACCGTGAC CATAAGCGCT ACCTGGAAAC CTATATGAAG CCGTACCCGG GTTACTTCTT CTTCGGCGAC GGTGCGGCAC GTGACTATGA TGGTTACATG TGGATTAAGG GTCGTGTCGA TGATGTTATC AACGTGAGCG GTCACCGTCT GAGCACCGCA GAAGTGGAGT CCGCGCTGAT TCTGCATAAG GGTGTCGCTG AAACCGCTGT CGTCGGTTGC GCGGACGATC TGACGGGCCA AGCCGTTTAC GCCTTTGTGA CCATGAAGCC AGAGTTCGAT CTGAAGGCCA CCAAGGAAGC TGATCTGAGC AAAGAACTGG CAATCCAGGT GCGCAAGGTT ATCGGTCCGT TTGCAGCGCC GAAGAAAATC TATCTGGTTA GCGATTTGCC AAAGACTCGT TCCGGCAAGA TTATGCGTCG TGTGCTGAGA AAAATCGTGG CGGGTGAGGG TGACCAACTG GGTGATTTGA GCAGCATTGC GGACCCGCAG ATTGTTGAGG AAGTTAAGCA AAAAGTTACC GGCAGCGCCT AATGAGGTTG AGGTCTCACC CCCTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CCCCTGAGAC GCGTCAATCG AGTTCGTACC TAAGGGCGAC ACCCCCTAAT TAGCCCGGGC GAAAGGCCCA GTCTTTCGAC TGAGCCTTTC GTTTTATTTG ATGCCTGGCA GTTCCCTACT CTCGCATGGG GAGTCCCCAC ACTACCATCG GCGCTACGGC GTTTCACTTC TGAGTTCGGC ATGGGGTCAG GTGGGACCAC CGCGCTACTG CCGCCAGGCA AACAAGGGGT GTTATGAGCC ATATTCAGGT ATAAATGGGC TCGCGATAAT GTTCAGAATT GGTTAATTGG TTGTAACACT GACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAAT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCACTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC GGAAAAACAG CGTTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAC TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGCCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA GCGGCGCGCC ATCGAATGGC GCAAAACCTT TCGCGGTATG GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATATGAAACC AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA TGAGGACGGT ACGCGACTGG GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG CGCTGGGCGC AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GATATCTCGG TAGTGGGATA CGACGATACC GAAGATAGCT CATGTTATAT CCCGCCGTTA ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC CAGTCTCACT GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGA
Details for CrneC.00629.a.HA11.PD00407
| PURIFICATION DATe: | 10/15/2015 |
| CONCENTRATION: | 20.2mg/ml |
| OBSERVED MW: | 76kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 10mM TRIS pH 8.5, 20mM NaCl |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 92 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MHHHHHHHHE NLYFQGPNYE SYVKEWAKTV GPNSDEWWAA KARETLDWYD DFKTVRAGGF EHGDVQWFPE GTLNAAYNCL DRHYYKNPKK TAIIYEADEP SESREVSYEE LMQETCRVAN VLKSYGVKKG DAVSIYLPMT WQAAAAFLAC ARIGAIHSAV FAGFSAESLR DRVNDCECKV LITTDEGRRG GKTIATKQIV DAALQQCPLV ENVLVLRRTG NKVPMTEGRD KWWDEECAKM PAYCPCERMA SEDPLFILYT SGSTGKPKGV VHSTAGYLLG TALTLKYVFD AHPDDRFACM ADIGWITGHS YIIYGPLANG ITTAVFESTP VYPTPSRYWD FVDKWKATQL YTAPTAIRLL RRMGEDHVKN HDLSSLRVLG SVGEPINPEA WHWYNDFAGK NQCAIVDTYW MTETGSISIA PLPGAISTKP GSATFPFFGM DVDIIDPQTG QVLEGNDVEG VLVARRPWPS IARTVYRDHK RYLETYMKPY PGYFFFGDGA ARDYDGYMWI KGRVDDVINV SGHRLSTAEV ESALILHKGV AETAVVGCAD DLTGQAVYAF VTMKPEFDLK ATKEADLSKE LAIQVRKVIG PFAAPKKIYL VSDLPKTRSG KIMRRVLRKI VAGEGDQLGD LSSIADPQIV EEVKQKVTG
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGCACCATC ACCACCACCA TCATCACGAG AATCTGTACT TCCAAGGCCC GAACTACGAG AGCTACGTCA AGGAATGGGC TAAAACGGTC GGCCCGAACA GCGATGAGTG GTGGGCAGCG AAAGCTCGCG AAACCCTGGA TTGGTATGAC GACTTTAAGA CCGTGCGTGC CGGTGGCTTC GAGCACGGTG ACGTGCAATG GTTCCCTGAG GGTACCCTGA ATGCCGCGTA CAATTGTTTG GATCGTCACT ATTACAAGAA TCCGAAGAAA ACCGCGATCA TTTACGAAGC GGATGAGCCG AGCGAGAGCC GCGAAGTGTC CTATGAAGAA CTGATGCAAG AAACCTGTCG TGTTGCGAAC GTTCTGAAAA GCTATGGCGT CAAGAAAGGT GACGCAGTGT CTATCTATCT CCCGATGACC TGGCAGGCAG CCGCGGCGTT TCTGGCCTGT GCGCGCATTG GTGCGATCCA TTCGGCAGTG TTCGCTGGCT TCAGCGCGGA GAGCCTGCGC GACCGTGTCA ATGACTGCGA GTGCAAAGTG CTGATCACCA CCGATGAGGG CCGTCGCGGT GGCAAAACGA TCGCAACGAA ACAGATCGTC GATGCGGCAC TGCAGCAGTG CCCGTTGGTT GAAAATGTGT TGGTTCTGCG TCGCACGGGT AACAAAGTTC CGATGACCGA GGGTCGTGAC AAGTGGTGGG ATGAAGAGTG CGCGAAAATG CCGGCGTATT GCCCGTGTGA GCGTATGGCT TCCGAGGACC CGCTGTTTAT TCTGTACACC AGCGGTAGCA CTGGCAAGCC GAAAGGTGTG GTGCATAGCA CCGCTGGTTA TCTGCTGGGT ACCGCGCTGA CCCTGAAATA TGTTTTTGAT GCGCATCCGG ACGACCGTTT CGCCTGCATG GCAGATATTG GTTGGATTAC TGGCCACAGC TACATTATCT ACGGCCCTCT GGCCAACGGT ATCACGACGG CAGTCTTTGA GAGCACCCCG GTGTATCCGA CGCCGAGCCG TTATTGGGAC TTCGTCGATA AATGGAAAGC GACGCAGCTG TACACCGCCC CAACGGCGAT CCGTTTATTG CGCCGCATGG GTGAGGACCA TGTTAAGAAT CACGATCTGA GCTCTCTGCG TGTTTTGGGT AGCGTCGGCG AGCCGATCAA CCCAGAGGCG TGGCATTGGT ACAACGATTT CGCAGGCAAA AATCAGTGTG CAATTGTGGA CACCTACTGG ATGACGGAAA CGGGCTCTAT CAGCATTGCC CCGCTGCCTG GCGCGATTAG CACCAAACCG GGTTCGGCGA CTTTTCCGTT TTTCGGTATG GACGTCGACA TTATTGACCC GCAGACTGGC CAAGTTCTTG AGGGTAACGA CGTCGAAGGC GTGCTGGTTG CTCGCCGTCC GTGGCCGAGC ATCGCGCGCA CGGTCTACCG TGACCATAAG CGCTACCTGG AAACCTATAT GAAGCCGTAC CCGGGTTACT TCTTCTTCGG CGACGGTGCG GCACGTGACT ATGATGGTTA CATGTGGATT AAGGGTCGTG TCGATGATGT TATCAACGTG AGCGGTCACC GTCTGAGCAC CGCAGAAGTG GAGTCCGCGC TGATTCTGCA TAAGGGTGTC GCTGAAACCG CTGTCGTCGG TTGCGCGGAC GATCTGACGG GCCAAGCCGT TTACGCCTTT GTGACCATGA AGCCAGAGTT CGATCTGAAG GCCACCAAGG AAGCTGATCT GAGCAAAGAA CTGGCAATCC AGGTGCGCAA GGTTATCGGT CCGTTTGCAG CGCCGAAGAA AATCTATCTG GTTAGCGATT TGCCAAAGAC TCGTTCCGGC AAGATTATGC GTCGTGTGCT GAGAAAAATC GTGGCGGGTG AGGGTGACCA ACTGGGTGAT TTGAGCAGCA TTGCGGACCC GCAGATTGTT GAGGAAGTTA AGCAAAAAGT TACCGGCTGA TAAGCTTGCG GCCGCACTCG AGCACCACCA CCACCACCAC TGAGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGAT
Details for CrneC.00629.a.FS11.PD00460
| PURIFICATION DATe: | 9/21/2016 |
| CONCENTRATION: | 10.07mg/ml |
| OBSERVED MW: | 77kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 10mM tris pH 8.5, 20mM NaCl |
| EXPRESSION HOST: | Data not available |
| VIAL COUNT (approx.): | 220 |
| VIAL VOLUME: | 220µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MHHHHHHHHE NLYFQGKTEV APGVHHVHPL PDSVPESEDL FAPPPRMQGK EGRPKPHIGP NYESYVKEWA KTVGPNSDEW WAAKARETLD WYDDFKTVRA GGFEHGDVQW FPEGTLNAAY NCLDRHYYKN PKKTAIIYEA DEPSESREVS YEELMQETCR VANVLKSYGV KKGDAVSIYL PMTWQAAAAF LACARIGAIH SAVFAGFSAE SLRDRVNDCE CKVLITTDEG RRGGKTIATK QIVDAALQQC PLVENVLVLR RTGNKVPMTE GRDKWWDEEC AKMPAYCPCE RMASEDPLFI LYTSGSTGKP KGVVHSTAGY LLGTALTLKY VFDAHPDDRF ACMADIGWIT GHSYIIYGPL ANGITTAVFE STPVYPTPSR YWDFVDKWKA TQLYTAPTAI RLLRRMGEDH VKNHDLSSLR VLGSVGEPIN PEAWHWYNDF AGKNQCAIVD TYWMTETGSI SIAPLPGAIS TKPGSATFPF FGMDVDIIDP QTGQVLEGND VEGVLVARRP WPSIARTVYR DHKRYLETYM KPYPGYFFFG DGAARDYDGY MWIKGRVDDV INVSGHRLST AEVESALILH KGVAETAVVG CADDLTGQAV YAFVTMKPEF DLKATKEADL SKELAIQVRK VIGPFAAPKK IYLVSDLPKT RSGKIMRRVL RKIVAGEGDQ LGDLSSIADP QIVEEVKQKV TGSA
Full NT Sequence (Expression Vector + Insert)
TTAATAAGAT GATCTTCTTG AGATCGTTTT GGTCTGCGCG TAATCTCTTG CTCTGAAAAC GAAAAAACCG CCTTGCAGGG CGGTTTTTCG AAGGTTCTCT GAGCTACCAA CTCTTTGAAC CGAGGTAACT GGCTTGGAGG AGCGCAGTCA CCAAAACTTG TCCTTTCAGT TTAGCCTTAA CCGGCGCATG ACTTCAAGAC TAACTCCTCT AAATCAATTA CCAGTGGCTG CTGCCAGTGG TGCTTTTGCA TGTCTTTCCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGACTGA ACGGGGGGTT CGTGCATACA GTCCAGCTTG GAGCGAACTG CCTACCCGGA ACTGAGTGTC AGGCGTGGAA TGAGACAAAC GCGGCCATAA CAGCGGAATG ACACCGGTAA ACCGAAAGGC AGGAACAGGA GAGCGCACGA GGGAGCCGCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCACT GATTTGAGCG TCAGATTTCG TGATGCTTGT CAGGGGGGCG GAGCCTATGG AAAAACGGCT TTGCCGCGGC CCTCTCACTT CCCTGTTAAG TATCTTCCTG GCATCTTCCA GGAAATCTCC GCCCCGTTCG TAAGCCATTT CCGCTCGCCG CAGTCGAACG ACCGAGCGTA GCGAGTCAGT GAGCGAGGAA GCGGAATATA TCCTGTATCA CATATTCTGC TGACGCACCG GTGCAGCCTT TTTTCTCCTG CCACATGAAG CACTTCACTG ACACCCTCAT CAGTGCCAAC ATAGTAAGCC AGTATACACT CCGCTAGCGC TGAGGTCCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGGCG AGAGTAGGGA ACTGCCAGGC ATCAAACTAA GCAGAAGGCC CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTCT GTGTTGTAAA ACGACGGCCA GTCTTAAGCT CGGGCCCCCT GGGCGGTTCT GATAACGAGT AATCGTTAAT CCGCAAATAA CGTAAAAACC CGCTTCGGCG GGTTTTTTTA TGGGGGGAGT TTAGGGAAAG AGCATTTGTC AGAATATTTA AGGGCGCCTG TCACTTTGCT TGATATATGA GAATTATTTA ACCTTATAAA TGAGAAAAAA GCAACGCACT TTAAATAAGA TACGTTGCTT TTTCGATTGA TGAACACCTA TAATTAAACT ATTCATCTAT TATTTATGAT TTTTTGTATA TACAATATTT CTAGTTTGTT AAAGAGAATT AAGAAAATAA ATCTCGAAAA TAATAAAGGG AAAATCAGTT TTTGATATCA AAATTATACA TGTCAACGAT AATACAAAAT ATAATACAAA CTATAAGATG TTATCAGTAT TTATTATGCA TTTAGAATAA ATTTTGTGTC GCCCTTCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC CTCTAGAAAT AATTTTGTTT AACTTTTAGA GACCAAGGAG GTAAAAAATG CATCACCACC ACCATCACCA CCACGAAAAT CTGTACTTCC AAGGTAAGAC CGAAGTCGCA CCGGGTGTTC ACCACGTTCA CCCACTGCCG GATTCAGTTC CGGAATCCGA GGACCTGTTT GCACCGCCTC CGCGTATGCA AGGCAAAGAG GGCCGTCCGA AACCGCACAT TGGCCCGAAC TACGAGAGCT ACGTCAAGGA ATGGGCTAAA ACGGTCGGCC CGAACAGCGA TGAGTGGTGG GCAGCGAAAG CTCGCGAAAC CCTGGATTGG TATGACGACT TTAAGACCGT GCGTGCCGGT GGCTTCGAGC ACGGTGACGT GCAATGGTTC CCTGAGGGTA CCCTGAATGC CGCGTACAAT TGTTTGGATC GTCACTATTA CAAGAATCCG AAGAAAACCG CGATCATTTA CGAAGCGGAT GAGCCGAGCG AGAGCCGCGA AGTGTCCTAT GAAGAACTGA TGCAAGAAAC CTGTCGTGTT GCGAACGTTC TGAAAAGCTA TGGCGTCAAG AAAGGTGACG CAGTGTCTAT CTATCTCCCG ATGACCTGGC AGGCAGCCGC GGCGTTTCTG GCCTGTGCGC GCATTGGTGC GATCCATTCG GCAGTGTTCG CTGGCTTCAG CGCGGAGAGC CTGCGCGACC GTGTCAATGA CTGCGAGTGC AAAGTGCTGA TCACCACCGA TGAGGGCCGT CGCGGTGGCA AAACGATCGC AACGAAACAG ATCGTCGATG CGGCACTGCA GCAGTGCCCG TTGGTTGAAA ATGTGTTGGT TCTGCGTCGC ACGGGTAACA AAGTTCCGAT GACCGAGGGT CGTGACAAGT GGTGGGATGA AGAGTGCGCG AAAATGCCGG CGTATTGCCC GTGTGAGCGT ATGGCTTCCG AGGACCCGCT GTTTATTCTG TACACCAGCG GTAGCACTGG CAAGCCGAAA GGTGTGGTGC ATAGCACCGC TGGTTATCTG CTGGGTACCG CGCTGACCCT GAAATATGTT TTTGATGCGC ATCCGGACGA CCGTTTCGCC TGCATGGCAG ATATTGGTTG GATTACTGGC CACAGCTACA TTATCTACGG CCCTCTGGCC AACGGTATCA CGACGGCAGT CTTTGAGAGC ACCCCGGTGT ATCCGACGCC GAGCCGTTAT TGGGACTTCG TCGATAAATG GAAAGCGACG CAGCTGTACA CCGCCCCAAC GGCGATCCGT TTATTGCGCC GCATGGGTGA GGACCATGTT AAGAATCACG ATCTGAGCTC TCTGCGTGTT TTGGGTAGCG TCGGCGAGCC GATCAACCCA GAGGCGTGGC ATTGGTACAA CGATTTCGCA GGCAAAAATC AGTGTGCAAT TGTGGACACC TACTGGATGA CGGAAACGGG CTCTATCAGC ATTGCCCCGC TGCCTGGCGC GATTAGCACC AAACCGGGTT CGGCGACTTT TCCGTTTTTC GGTATGGACG TCGACATTAT TGACCCGCAG ACTGGCCAAG TTCTTGAGGG TAACGACGTC GAAGGCGTGC TGGTTGCTCG CCGTCCGTGG CCGAGCATCG CGCGCACGGT CTACCGTGAC CATAAGCGCT ACCTGGAAAC CTATATGAAG CCGTACCCGG GTTACTTCTT CTTCGGCGAC GGTGCGGCAC GTGACTATGA TGGTTACATG TGGATTAAGG GTCGTGTCGA TGATGTTATC AACGTGAGCG GTCACCGTCT GAGCACCGCA GAAGTGGAGT CCGCGCTGAT TCTGCATAAG GGTGTCGCTG AAACCGCTGT CGTCGGTTGC GCGGACGATC TGACGGGCCA AGCCGTTTAC GCCTTTGTGA CCATGAAGCC AGAGTTCGAT CTGAAGGCCA CCAAGGAAGC TGATCTGAGC AAAGAACTGG CAATCCAGGT GCGCAAGGTT ATCGGTCCGT TTGCAGCGCC GAAGAAAATC TATCTGGTTA GCGATTTGCC AAAGACTCGT TCCGGCAAGA TTATGCGTCG TGTGCTGAGA AAAATCGTGG CGGGTGAGGG TGACCAACTG GGTGATTTGA GCAGCATTGC GGACCCGCAG ATTGTTGAGG AAGTTAAGCA AAAAGTTACC GGCAGCGCCT AATGAGGTTG AGGTCTCACC CCCTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CCCCTGAGAC GCGTCAATCG AGTTCGTACC TAAGGGCGAC ACCCCCTAAT TAGCCCGGGC GAAAGGCCCA GTCTTTCGAC TGAGCCTTTC GTTTTATTTG ATGCCTGGCA GTTCCCTACT CTCGCATGGG GAGTCCCCAC ACTACCATCG GCGCTACGGC GTTTCACTTC TGAGTTCGGC ATGGGGTCAG GTGGGACCAC CGCGCTACTG CCGCCAGGCA AACAAGGGGT GTTATGAGCC ATATTCAGGT ATAAATGGGC TCGCGATAAT GTTCAGAATT GGTTAATTGG TTGTAACACT GACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAAT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCACTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC GGAAAAACAG CGTTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAC TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGCCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA GCGGCGCGCC ATCGAATGGC GCAAAACCTT TCGCGGTATG GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATATGAAACC AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA TGAGGACGGT ACGCGACTGG GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG CGCTGGGCGC AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GATATCTCGG TAGTGGGATA CGACGATACC GAAGATAGCT CATGTTATAT CCCGCCGTTA ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC CAGTCTCACT GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGA